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An investigation on distribution of borrelia transmitting of borrelia trasmitting ticks in daset i Gorgan and Turkemeashra, Iran
184
OraI Sessions I Parasitology
International
47 (Suppl.) (1998)
O-0265
CM263
PROPHYLAXIS AND CCNTROL KEASURES OF HOUSE DUST MITES IN UZBEKISTAN
Nazrullaeva Institute
Pwili, P.* Assnr ,H. * PiatakJ. * wekooii,Il. * Amirkbani,A.* * PasteurInstituteof Iran Maddaf,s. R. * A studyailing at deteniaiq distributionof medicallyimportant ticks andtheir potentialroles in translittiq Imrreliaawascarried out by the Parasitolqydivision0f PasteurInstituteof Iran, duriq 1996-1997. TheCKDS~ sectionalmethod wasadopted for aa&%iq and the ticks uerecollected frw stables, b\oaadwlliq buildin@, bird nests, tattles androdents’buxrws. Rle collectedsamples uexekept in glass tube9ard transferred to the laboratoryfor furtherstudies. The qewra Aqas and 0rnith0d0ravbeloqiq to the fUily Aqasidaeatd generaB00@lps,Mn0phyallis, Byalolla,DerWxntOr and Rbipicqtilalus belonging to the familyIx0dida0 wereidentified. tickdborneorreliosis are CMLsed $ differentspeciesof IkxreIias andtransnittedaa0q r0dantsby a varietyof ticks. lhman is jUst anoccassioaalintndu in the diseasecycle. hll 210 mdbod~rou~ tartdkovskiwe c4llectedfros miosbosis _n&i burrowsti&~vidually fed on infantI&oratory Iice sfti?r five &ys, a blood saqle wastaken frol SIIiMh and era&d by darkfield ~icr0sc0py. 88 of tbet yete positive. TO deterninethe Borrelliaspecies imrolvad,MnCa pigs, adult aad infantlice andinfat rabbitsuexeiwcrrlatedvitb irkted blood. ms results ~ttmgly confined tbst ths species involvedws B. latysiwii.
0-0264
zOONOTIC SURVEYFORLYMEDISEASEINFECTION IN TAIWANAREA
Shih CMand Chao LL Departmentof Parasitology and Tropical Medicine, National Defense Medical Center, Taipei. Taiwan, R.0.C Lyme disease is a tick-transmitted zoonotic infection and the etiological agent, Borrelia burgdorferi sensu late, for Lyme disease was perpetuated in the natural hosts of wild and peridomestic rodents. Thus, we conducted a zconotic survey to investigate the spirochetal infection in small mammals. Ear tissues of trapped ro. dents collected from various localities of Taiwan were incubated into BSK~H medium and examined for the evidence of spirochetal infection by dark-field microscopy. Spirochetes can be isolated from six species of rodents and seven isolates, designated TNKMI -7, were purified. The overall infection rate was 16.6% (53/320) of captured rodents and the highest infection rate (36.4%) was detected in the brown country rat (Rattus losea, Swinhoe) -captured from the eastern localities and offshore island. Reactivity with B. burgdorferi--specific qonoclonal anti& bodies indicates that the identity of Taiwan isolates was This study closely related to the agent of Lyme disease. provides the first evidence of existence of Lymedisease spirochetes in Taiwan area.
133-281
of
Id. Zoology,
Tashkent,
Uzbekistan
We studied the effect of sunlight on vital activity of house dust mites. So, a culture
of live mites D.pteronyssinus was exposed to direct sunlight at the air to 30-32%.Various substrata were placed in Petri dishes, wooden boxes and filter paper. In all cases, the mites died and got stuck to the substrata after five-minute-long exposition. Further, such bedding as pillows, blankets and matrasses was exposed to direct sunlight. However, it took longer time for the mites to die, which was because of the thickness of padding. We also studied on the effect of boracic acid and NaHCO substances easily available, on the vital a a’tivitg of the mites D.pteronyssinus. All the mites were affected by the fifteen-minute-long exposition to 5% boracic acid. Seventy-five per cent of the mites died and the rest were paralysed. When exposed to % NaHCO the mites died in 2 hours post treatmen5: No egg hatching was recorded in Petri dishes for a month and more after the trials involving both the boracic acid and NaHCO He%e, under conditions of Uzbekistan, where the solar radiation is high for six months a year, sunlight may be recommended as the major tool of controlling house dust mites, and the boracic acid and household as the additional ones.
o-0266
SERO-EPIDEI’IIOLOGY OF BANCROFTIAN FILARIOSIS IN A COLLIERY AREA OF WEST BENGAL, INDIA
Haldar J.P. Bangabasive
and Adhikari P. College, Calcutta-700
009,India.
A total number of 47,122 people living in a colliery area of Burdwan district, iJest Bengal, an endemic zone of filariosis, were examined for clinico-positivity, endemicity, microfilaria(mf)-positivity, mf-clinicopositivity and mf-density. The same parameters were also examined in 47,672 persons living in the non-colliery area of the same district. The density of the vectors. along with infection and infectivity rates in two areas were determined. All these epidemiological and parasitological parameters were found to be significantly higher in the colliery area when compared to non-colliery one. The high vector density along with other environmental factors like exposure of the host to the coal might influence the higher rate of filariosis in colliery areas. An age-sex analysis of the patients revealed that the number of filariosis cases were significantly lower among the females belonging to 16-35 years of age group, as compared to the males of the same age group. Filarial antibodies could be detected in the sera of microfilaremic asymptomatic and amikrofilaremic symptomatic (elephantiasis) patients. IgG4 and 18Gi isotypes of antibodies were found to be dominant in the former and latter cases respectively. This indicates that the active infection promotes the production of IgG4 isotype, while the development of pathology and the elimination of circulating Parasites is associated with higher IgGl isotype.
OraI Sessions I Parasitology
International
47 (Suppl.) (1998)
O-0265
CM263
PROPHYLAXIS AND CCNTROL KEASURES OF HOUSE DUST MITES IN UZBEKISTAN
Nazrullaeva Institute
Pwili, P.* Assnr ,H. * PiatakJ. * wekooii,Il. * Amirkbani,A.* * PasteurInstituteof Iran Maddaf,s. R. * A studyailing at deteniaiq distributionof medicallyimportant ticks andtheir potentialroles in translittiq Imrreliaawascarried out by the Parasitolqydivision0f PasteurInstituteof Iran, duriq 1996-1997. TheCKDS~ sectionalmethod wasadopted for aa&%iq and the ticks uerecollected frw stables, b\oaadwlliq buildin@, bird nests, tattles androdents’buxrws. Rle collectedsamples uexekept in glass tube9ard transferred to the laboratoryfor furtherstudies. The qewra Aqas and 0rnith0d0ravbeloqiq to the fUily Aqasidaeatd generaB00@lps,Mn0phyallis, Byalolla,DerWxntOr and Rbipicqtilalus belonging to the familyIx0dida0 wereidentified. tickdborneorreliosis are CMLsed $ differentspeciesof IkxreIias andtransnittedaa0q r0dantsby a varietyof ticks. lhman is jUst anoccassioaalintndu in the diseasecycle. hll 210 mdbod~rou~ tartdkovskiwe c4llectedfros miosbosis _n&i burrowsti&~vidually fed on infantI&oratory Iice sfti?r five &ys, a blood saqle wastaken frol SIIiMh and era&d by darkfield ~icr0sc0py. 88 of tbet yete positive. TO deterninethe Borrelliaspecies imrolvad,MnCa pigs, adult aad infantlice andinfat rabbitsuexeiwcrrlatedvitb irkted blood. ms results ~ttmgly confined tbst ths species involvedws B. latysiwii.
0-0264
zOONOTIC SURVEYFORLYMEDISEASEINFECTION IN TAIWANAREA
Shih CMand Chao LL Departmentof Parasitology and Tropical Medicine, National Defense Medical Center, Taipei. Taiwan, R.0.C Lyme disease is a tick-transmitted zoonotic infection and the etiological agent, Borrelia burgdorferi sensu late, for Lyme disease was perpetuated in the natural hosts of wild and peridomestic rodents. Thus, we conducted a zconotic survey to investigate the spirochetal infection in small mammals. Ear tissues of trapped ro. dents collected from various localities of Taiwan were incubated into BSK~H medium and examined for the evidence of spirochetal infection by dark-field microscopy. Spirochetes can be isolated from six species of rodents and seven isolates, designated TNKMI -7, were purified. The overall infection rate was 16.6% (53/320) of captured rodents and the highest infection rate (36.4%) was detected in the brown country rat (Rattus losea, Swinhoe) -captured from the eastern localities and offshore island. Reactivity with B. burgdorferi--specific qonoclonal anti& bodies indicates that the identity of Taiwan isolates was This study closely related to the agent of Lyme disease. provides the first evidence of existence of Lymedisease spirochetes in Taiwan area.
133-281
of
Id. Zoology,
Tashkent,
Uzbekistan
We studied the effect of sunlight on vital activity of house dust mites. So, a culture
of live mites D.pteronyssinus was exposed to direct sunlight at the air to 30-32%.Various substrata were placed in Petri dishes, wooden boxes and filter paper. In all cases, the mites died and got stuck to the substrata after five-minute-long exposition. Further, such bedding as pillows, blankets and matrasses was exposed to direct sunlight. However, it took longer time for the mites to die, which was because of the thickness of padding. We also studied on the effect of boracic acid and NaHCO substances easily available, on the vital a a’tivitg of the mites D.pteronyssinus. All the mites were affected by the fifteen-minute-long exposition to 5% boracic acid. Seventy-five per cent of the mites died and the rest were paralysed. When exposed to % NaHCO the mites died in 2 hours post treatmen5: No egg hatching was recorded in Petri dishes for a month and more after the trials involving both the boracic acid and NaHCO He%e, under conditions of Uzbekistan, where the solar radiation is high for six months a year, sunlight may be recommended as the major tool of controlling house dust mites, and the boracic acid and household as the additional ones.
o-0266
SERO-EPIDEI’IIOLOGY OF BANCROFTIAN FILARIOSIS IN A COLLIERY AREA OF WEST BENGAL, INDIA
Haldar J.P. Bangabasive
and Adhikari P. College, Calcutta-700
009,India.
A total number of 47,122 people living in a colliery area of Burdwan district, iJest Bengal, an endemic zone of filariosis, were examined for clinico-positivity, endemicity, microfilaria(mf)-positivity, mf-clinicopositivity and mf-density. The same parameters were also examined in 47,672 persons living in the non-colliery area of the same district. The density of the vectors. along with infection and infectivity rates in two areas were determined. All these epidemiological and parasitological parameters were found to be significantly higher in the colliery area when compared to non-colliery one. The high vector density along with other environmental factors like exposure of the host to the coal might influence the higher rate of filariosis in colliery areas. An age-sex analysis of the patients revealed that the number of filariosis cases were significantly lower among the females belonging to 16-35 years of age group, as compared to the males of the same age group. Filarial antibodies could be detected in the sera of microfilaremic asymptomatic and amikrofilaremic symptomatic (elephantiasis) patients. IgG4 and 18Gi isotypes of antibodies were found to be dominant in the former and latter cases respectively. This indicates that the active infection promotes the production of IgG4 isotype, while the development of pathology and the elimination of circulating Parasites is associated with higher IgGl isotype.