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Analysis of medical records of patients hospitalized in the Clinical Hospital of Przemienienia Pańskiego in Poznan
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Abstracts – XIX Congress PTF / Pharmacological Reports 67S (2015) 2–45
mouse MES model. The utmost caution is required when combining xanthotoxin with pregabalin due to the acute adverse effects (impairment of motor coordination) that might appear. Xanthotoxin as a component of supplementary diet would contribute to seizure suppression in epileptic patients inadequately medicated with second-generation antiepileptic drugs, if the results from this study could be translated into clinical settings. http://dx.doi.org/10.1016/j.pharep.2015.06.078 Effect of 2-methyl-6-(phenylethynyl)pyridine hydrochloride (MPEP) on the anticonvulsant action of lamotrigine, oxcarbazepine, pregabalin and topiramate in the maximal electroshockinduced seizure test in mice Maria W. Kondrat-Wro´bel 1, Dorota Z˙o´łkowska 2, Jarogniew J. Łuszczki 1,3 1
Department of Pathophysiology, Medical University, Lublin, Poland Department of Neurology, School of Medicine, University of California, Davis, Sacramento, CA, USA 3 Isobolographic Analysis Laboratory, Institute of Rural Health, Lublin, Poland 2
The aim of this study was to evaluate the effects of 2-methyl-6(phenylethynyl)pyridine hydrochloride (MPEP – a potent and highly selective non-competitive antagonist at the mGlu5 receptor subtype and a positive allosteric modulator at mGlu4 receptors) on the anticonvulsant potency of four second-generation antiepileptic drug (lamotrigine [LTG], oxcarbazepine [OXC], pregabalin [PGB] and topiramate [TPM]) against maximal electroshock-induced tonic seizures (MES) in mice. Tonic hindlimb extension (seizure activity) was evoked in albino Swiss mice by a current (25 mA, 500 V, 50 Hz, 0.2 s stimulus duration) delivered via ear-clip electrodes. MPEP was administered intraperitoneally (ip) in increasing doses of 0.5–2.0 mg/kg, so as to determine its influence on the threshold for electroconvulsions in mice. The antiepileptic drugs (LTG, OXC, PGB and TPM) were administered ip either alone or in combination with MPEP in increasing doses so as to determine their median effective doses (ED50 values) in the MES test. Results indicate that MPEP in doses of 1.5 and 2 mg/kg significantly elevated (p < 0.05 and p < 0.001) the threshold for electroconvulsions in mice. MPEP administered ip at a dose of 1 mg/ kg significantly enhanced the anticonvulsant potency of pregabalin and topiramate (p < 0.05), but not that of lamotrigine or oxcarbazepine in the mouse MES model. In contrast, MPEP at a dose of 0.5 mg/kg had no impact on the anticonvulsant potency of all the studied antiepileptic drugs in the mouse MES model. In conclusions, MPEP as the potent and highly selective non-competitive antagonist of mGlu5 and the positive allosteric modulator of mGlu4 receptors potentiated the anticonvulsant action of PGB and TPM in the mouse MES model. The inhibition of mGlu5 receptors by MPEP in the brain might contribute to the reduction of seizures in experimental animals receiving PGB and TPM. http://dx.doi.org/10.1016/j.pharep.2015.06.079 Analysis of medical records of patients hospitalized in the Clinical Hospital of ´ skiego in Poznan Przemienienia Pan Katarzyna Korzeniowska 1, Ewa Kaz´mierczk 1, Iwona Andrys-Wawrzyniak 1, Iwona Smolarek 1, Artur Cies´lewicz 1, Anna Jabłecka 1, Hanna Gracz 2, Anna Głowacka 2, Jolanta Nagadowska 2
1
Department of Clinical Pharmacology, Poznan University of Medical Sciences, Poznan´, Poland 2 Clinical Hospital of Przemienienia Pan´skiego in Poznan´, Poznan´, Poland A major problem in both health and economic terms is the issue of polypharmacy and polypragmasia. There is no standard definition concerning polypragmasia. Polypharmacy is most commonly defined as receiving at least 5 drugs. Polypragmasia is sometimes referred as uptake by the patient at least one drug for which there is no indication or more drugs than is clinically indicated. Such an approach in the case of older people is sometimes limited due to occurring multidisease. The study presents the analysis of 120 case records of patients hospitalized in 2014 in 15 wards of the Hospital of Przemienienia Pan´skiego in Poznan´ (anesthesia, chemotherapy, surgery, gynecology, oncology, hematology, cardiology, palliative medicine, ophthalmology, pulmonological). 36 were randomly selected stories, 52 stories of patients whose pharmacotherapy consisted of 5 and more drugs and 32 stories of patients over 80 years of age. That assessment concerned the validity of used drugs and the prevalence of: polypharmacy, adverse effects and drug interactions. Analysis confirmed the validity of therapy. The use of appropriate treatment fit to patients’ health status and concurrent disease, helped to avoid clinically significant drug interactions and serious adverse effects. Registered adverse reactions were quickly diagnosed allowing for their effective treatment. http://dx.doi.org/10.1016/j.pharep.2015.06.080 Ketogenic diet suppresses spontaneous ethyl alcohol intake and reduces withdrawal syndrome in rats Paweł Krza˛s´cik 1, Wanda Dyr 2, Edyta Wyszogrodzka 2, Danuta Turzyn´ska 2, Alicja Sobolewska 2, Anna Sko´rzewska 2, Jerzy Walkowiak 2, Małgorzata Zajda 1, Adam Płaz´nik 1,2 1 Department of Experimental and Clinical Pharmacology, Medical University, Warszawa, Poland 2 Department of Pharmacology and Physiology of the Nervous System, Institute Psychiatry and Neurology, Warszawa, Poland
Objective: In the present study we analyzed the influence of orally administered basic ingredients of the ketogenic diet – saturated fatty acids: octanoic acid and decanonic acid (a mixture proportioned 89:11) on the influence of ethyl alcohol (EtOH) in rats. Methods: 1. Influence of saturated fatty acids on spontaneous intake of alcohol and sacharosis in rats with high alcohol preference (WHP). 2. Influence of saturated fatty acids on audiogenic seizure response (ASR) in EtOH withdrawal in rats. 3. Influence of saturated fatty acids on duration of sleep induced by intraperitoneal alcohol administration. 4. Influence of saturated fatty acids on the open field test. 5. Analysis of influence of saturated fatty acids on corticosterone plasma concentration in rats. 6. Analysis of influence of saturated fatty acids on NA, DA, 5-HT and their metabolites’ concentrations as well as concentrations of taurin, glutamine, glycine, alanine, aspargic acetate, glutaminergic acetate and GABA in frontal cortex, striatum, hippocampus and PVN tissue homogenates. Results: Saturated fatty acids dose-dependently suppressed spontaneous alcohol intake by WHP rats. They also suppressed
Abstracts – XIX Congress PTF / Pharmacological Reports 67S (2015) 2–45
mouse MES model. The utmost caution is required when combining xanthotoxin with pregabalin due to the acute adverse effects (impairment of motor coordination) that might appear. Xanthotoxin as a component of supplementary diet would contribute to seizure suppression in epileptic patients inadequately medicated with second-generation antiepileptic drugs, if the results from this study could be translated into clinical settings. http://dx.doi.org/10.1016/j.pharep.2015.06.078 Effect of 2-methyl-6-(phenylethynyl)pyridine hydrochloride (MPEP) on the anticonvulsant action of lamotrigine, oxcarbazepine, pregabalin and topiramate in the maximal electroshockinduced seizure test in mice Maria W. Kondrat-Wro´bel 1, Dorota Z˙o´łkowska 2, Jarogniew J. Łuszczki 1,3 1
Department of Pathophysiology, Medical University, Lublin, Poland Department of Neurology, School of Medicine, University of California, Davis, Sacramento, CA, USA 3 Isobolographic Analysis Laboratory, Institute of Rural Health, Lublin, Poland 2
The aim of this study was to evaluate the effects of 2-methyl-6(phenylethynyl)pyridine hydrochloride (MPEP – a potent and highly selective non-competitive antagonist at the mGlu5 receptor subtype and a positive allosteric modulator at mGlu4 receptors) on the anticonvulsant potency of four second-generation antiepileptic drug (lamotrigine [LTG], oxcarbazepine [OXC], pregabalin [PGB] and topiramate [TPM]) against maximal electroshock-induced tonic seizures (MES) in mice. Tonic hindlimb extension (seizure activity) was evoked in albino Swiss mice by a current (25 mA, 500 V, 50 Hz, 0.2 s stimulus duration) delivered via ear-clip electrodes. MPEP was administered intraperitoneally (ip) in increasing doses of 0.5–2.0 mg/kg, so as to determine its influence on the threshold for electroconvulsions in mice. The antiepileptic drugs (LTG, OXC, PGB and TPM) were administered ip either alone or in combination with MPEP in increasing doses so as to determine their median effective doses (ED50 values) in the MES test. Results indicate that MPEP in doses of 1.5 and 2 mg/kg significantly elevated (p < 0.05 and p < 0.001) the threshold for electroconvulsions in mice. MPEP administered ip at a dose of 1 mg/ kg significantly enhanced the anticonvulsant potency of pregabalin and topiramate (p < 0.05), but not that of lamotrigine or oxcarbazepine in the mouse MES model. In contrast, MPEP at a dose of 0.5 mg/kg had no impact on the anticonvulsant potency of all the studied antiepileptic drugs in the mouse MES model. In conclusions, MPEP as the potent and highly selective non-competitive antagonist of mGlu5 and the positive allosteric modulator of mGlu4 receptors potentiated the anticonvulsant action of PGB and TPM in the mouse MES model. The inhibition of mGlu5 receptors by MPEP in the brain might contribute to the reduction of seizures in experimental animals receiving PGB and TPM. http://dx.doi.org/10.1016/j.pharep.2015.06.079 Analysis of medical records of patients hospitalized in the Clinical Hospital of ´ skiego in Poznan Przemienienia Pan Katarzyna Korzeniowska 1, Ewa Kaz´mierczk 1, Iwona Andrys-Wawrzyniak 1, Iwona Smolarek 1, Artur Cies´lewicz 1, Anna Jabłecka 1, Hanna Gracz 2, Anna Głowacka 2, Jolanta Nagadowska 2
1
Department of Clinical Pharmacology, Poznan University of Medical Sciences, Poznan´, Poland 2 Clinical Hospital of Przemienienia Pan´skiego in Poznan´, Poznan´, Poland A major problem in both health and economic terms is the issue of polypharmacy and polypragmasia. There is no standard definition concerning polypragmasia. Polypharmacy is most commonly defined as receiving at least 5 drugs. Polypragmasia is sometimes referred as uptake by the patient at least one drug for which there is no indication or more drugs than is clinically indicated. Such an approach in the case of older people is sometimes limited due to occurring multidisease. The study presents the analysis of 120 case records of patients hospitalized in 2014 in 15 wards of the Hospital of Przemienienia Pan´skiego in Poznan´ (anesthesia, chemotherapy, surgery, gynecology, oncology, hematology, cardiology, palliative medicine, ophthalmology, pulmonological). 36 were randomly selected stories, 52 stories of patients whose pharmacotherapy consisted of 5 and more drugs and 32 stories of patients over 80 years of age. That assessment concerned the validity of used drugs and the prevalence of: polypharmacy, adverse effects and drug interactions. Analysis confirmed the validity of therapy. The use of appropriate treatment fit to patients’ health status and concurrent disease, helped to avoid clinically significant drug interactions and serious adverse effects. Registered adverse reactions were quickly diagnosed allowing for their effective treatment. http://dx.doi.org/10.1016/j.pharep.2015.06.080 Ketogenic diet suppresses spontaneous ethyl alcohol intake and reduces withdrawal syndrome in rats Paweł Krza˛s´cik 1, Wanda Dyr 2, Edyta Wyszogrodzka 2, Danuta Turzyn´ska 2, Alicja Sobolewska 2, Anna Sko´rzewska 2, Jerzy Walkowiak 2, Małgorzata Zajda 1, Adam Płaz´nik 1,2 1 Department of Experimental and Clinical Pharmacology, Medical University, Warszawa, Poland 2 Department of Pharmacology and Physiology of the Nervous System, Institute Psychiatry and Neurology, Warszawa, Poland
Objective: In the present study we analyzed the influence of orally administered basic ingredients of the ketogenic diet – saturated fatty acids: octanoic acid and decanonic acid (a mixture proportioned 89:11) on the influence of ethyl alcohol (EtOH) in rats. Methods: 1. Influence of saturated fatty acids on spontaneous intake of alcohol and sacharosis in rats with high alcohol preference (WHP). 2. Influence of saturated fatty acids on audiogenic seizure response (ASR) in EtOH withdrawal in rats. 3. Influence of saturated fatty acids on duration of sleep induced by intraperitoneal alcohol administration. 4. Influence of saturated fatty acids on the open field test. 5. Analysis of influence of saturated fatty acids on corticosterone plasma concentration in rats. 6. Analysis of influence of saturated fatty acids on NA, DA, 5-HT and their metabolites’ concentrations as well as concentrations of taurin, glutamine, glycine, alanine, aspargic acetate, glutaminergic acetate and GABA in frontal cortex, striatum, hippocampus and PVN tissue homogenates. Results: Saturated fatty acids dose-dependently suppressed spontaneous alcohol intake by WHP rats. They also suppressed