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Analysis of the biofilm formation of Candida albicans after Photodithazine®-mediated photodynamic therapy in vivo
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Abstracts / Photodiagnosis and Photodynamic Therapy 12 (2015) 325–375
Prevalence, virulence and sensitivity to antimicrobial therapy of strains of Enterococcus faecalis and Enterococcus faecium isolated from endodontic infections
of C. albicans recovered from the oral lesions of mice with oral candidiasis. http://dx.doi.org/10.1016/j.pdpdt.2015.07.055
Ana Carolina Chipoletti Santos, Jessica Diane dos Santos, Patrícia Pimentel de Barros, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira
Clinical and histopathological outcomes of one session of photodynamic therapy with previous CO2 laser application for actinic cheilitis
Biopatologia Bucal/Instituto de Ciência e Tecnologia, UNESP – Univ Estadual Paulista, São José dos Campos, SP, Brazil
Karla Bianca F. da C. Fontes 1 , Taiana C. Leite 1 , Ana Maria O. Miranda 1 , Maria Claudia A. Issa 1 , Eliane P. Dias 1 , Cristina Kurachi 2 , Licínio Esmeraldo da Silva 1 , Karin G.S. Cunha 1
Enterococcus faecium became one of the most feared microorganisms in hospital infections, with this study to isolate and identify E. faecalis and E. faecium in root canals with endodontic infections and compare these strains in sensitivity to conventional antibiotics and photodynamic therapy antimicrobial (PDT). In addition, these species were compared for virulence in vivo, using the model of experimental infection of Galleria mellonella. The strains of E. faecalis and E. faecium isolated from root canals were identified by the system Rapid ID 32 Strep and multiplex PCR. All isolates identified as E. faecalis and E. faecium were tested for antibiotic sensitivity by the E-test method for determination of minimal inhibitory concentration (MIC). Of these patients, all showed E. faecalis and only 2 had mixed infections with E. faecalis and E. faecium. Found 6 isolates (27%) E. faecalis resistant to antibiotics, and tetracycline resistant 3, 2 and 1 ciprofloxacin azithromycin. Isolates of E. faecium showed no resistance to the antibiotics tested. All isolates of E. faecalis and E. faecium tested were sensitive to photodynamic therapy microbial reduction of 95 99%. E. faecalis both as E. faecium to the virulence in G. mellonella was dependent from these strains. http://dx.doi.org/10.1016/j.pdpdt.2015.07.054 Analysis of the biofilm formation of Candida albicans after Photodithazine® -mediated photodynamic therapy in vivo Juliana Cabrini Carmello, Gabriela Maria Balista Ferreira, Fernanda Alves, Ewerton Garcia de Oliveira Mima, Janaina Habib Jorge, Ana Cláudia Pavarina Department of Dental Materials and Prosthodontics, Araraquara Dental School, Univ Estadual Paulista – UNESP, Brazil This study evaluated the capacity of biofilm formation of C. albicans recovered from the tongues of murine model of oral candidosis1, after consecutive applications of Photodithazine® (PDZ) (100 mg/L) associated with LED (660 nm) light (37.5 J/cm2 ). Mice with oral candidosis were treated with photodynamic therapy (PDT) and Nystatin (NYS). Next, fungal cells were recovered from the oral lesions and submitted to biofilm formation. The capacity of biofilm formation was analyzed by the cellular viability (CFU/mL), XTT assay and Violet Crystal staining assay (CV). Data were analyzed by ANOVA and Tukey post hoc (p < 0.05). PDT and NYS promoted a significant reduction in the biofilm formation of C. albicans in all tests performed, when compared to the control groups (p < 0.05). The cell viability reduced 1.6 and 2 log 10, for the groups treated with PDT and NYS, respectively. The XTT assay revealed 58% and 62% of reduction in the metabolic activity and the CV staining assay showed 82,4% and 77,5% of reduction in the total biomass, for the groups treated with PDT and NYS respectively. PDZmediated PDT treatment reduced the capacity of biofilm formation
1 Fluminense Federal University – UFF – Niterói – Rio de Janeiro, Brazil 2 São Carlos Institute of Physics, University of São Paulo, Brazil
Actinic Cheilitis (AC) is a common pre-cancerous lesion of the lip vermilion in response to chronic solar exposure. Microscopically, various degrees of epithelial dysplasia (ED) may be encountered. Photodynamic therapy (PDT) has been widely used in Dermatology, but its efficacy for AC is still controversial. The use of CO2 laser before PDT for cutaneous lesions has been shown to allow more uniform distribution of the photosensitizer and to improve its absorption. Our aim was to evaluate the efficacy of one session of PDT for AC with previous application of CO2 laser. Methods: Eight patients with AC of the lower lip were treated with one session of 20% methyl-aminolevulinate cream and red LED light source with previous fractioned ablative CO2 laser application. The outcomes were evaluated after a six-month period. Clinical alterations were mapped and registered before and after treatment. Histopathological outcomes were evaluated based on the presence and grading of ED. Results: All patients showed clinical improvement after PDT. Histopathological analysis showed that 66.6% of patients presented improvement of ED after PDT. Conclusions: One session of PDT with previous CO2 laser application, however further studies are needed in order to confirm this protocol’s efficacy. http://dx.doi.org/10.1016/j.pdpdt.2015.07.056 Photodynamic killing of cancer cells by a platinum-based compound, a derivative of Pt(II) dipyridobenzene Rachel E. Doherty, Igor V. Sazanovich, Luke K. McKenzie, Alexander S. Stasheuski, Rachel Coyle, Elizabeth Baggaley, Sarah Bottomley, Julia A. Weinstein, Helen E. Bryant Academic Unit of Molecular Oncology, Department of Oncology, University of Sheffield, United Kingdom The use of light-activated pro-drugs in cancer treatment is becoming increasingly popular due to their ability to selectively target tumour material whilst remaining non-toxic to healthy tissue. The study describes a novel platinum-based photosensitizer Pt(II) dipyridotolylchloride PtNCNMe. We demonstrate that activation of PtNCNMe (0.1–1 M) by light (405 nm) results in significant cell death of various cancer cell lines (including a cisplatin-resistant cell line). Treatment with neither light nor compound alone led to cell death. Furthermore, it is shown that PtNCNMe induces irreversible DNA single strand breaks following irradiation. In this way, we describe a promising new agent
Abstracts / Photodiagnosis and Photodynamic Therapy 12 (2015) 325–375
Prevalence, virulence and sensitivity to antimicrobial therapy of strains of Enterococcus faecalis and Enterococcus faecium isolated from endodontic infections
of C. albicans recovered from the oral lesions of mice with oral candidiasis. http://dx.doi.org/10.1016/j.pdpdt.2015.07.055
Ana Carolina Chipoletti Santos, Jessica Diane dos Santos, Patrícia Pimentel de Barros, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira
Clinical and histopathological outcomes of one session of photodynamic therapy with previous CO2 laser application for actinic cheilitis
Biopatologia Bucal/Instituto de Ciência e Tecnologia, UNESP – Univ Estadual Paulista, São José dos Campos, SP, Brazil
Karla Bianca F. da C. Fontes 1 , Taiana C. Leite 1 , Ana Maria O. Miranda 1 , Maria Claudia A. Issa 1 , Eliane P. Dias 1 , Cristina Kurachi 2 , Licínio Esmeraldo da Silva 1 , Karin G.S. Cunha 1
Enterococcus faecium became one of the most feared microorganisms in hospital infections, with this study to isolate and identify E. faecalis and E. faecium in root canals with endodontic infections and compare these strains in sensitivity to conventional antibiotics and photodynamic therapy antimicrobial (PDT). In addition, these species were compared for virulence in vivo, using the model of experimental infection of Galleria mellonella. The strains of E. faecalis and E. faecium isolated from root canals were identified by the system Rapid ID 32 Strep and multiplex PCR. All isolates identified as E. faecalis and E. faecium were tested for antibiotic sensitivity by the E-test method for determination of minimal inhibitory concentration (MIC). Of these patients, all showed E. faecalis and only 2 had mixed infections with E. faecalis and E. faecium. Found 6 isolates (27%) E. faecalis resistant to antibiotics, and tetracycline resistant 3, 2 and 1 ciprofloxacin azithromycin. Isolates of E. faecium showed no resistance to the antibiotics tested. All isolates of E. faecalis and E. faecium tested were sensitive to photodynamic therapy microbial reduction of 95 99%. E. faecalis both as E. faecium to the virulence in G. mellonella was dependent from these strains. http://dx.doi.org/10.1016/j.pdpdt.2015.07.054 Analysis of the biofilm formation of Candida albicans after Photodithazine® -mediated photodynamic therapy in vivo Juliana Cabrini Carmello, Gabriela Maria Balista Ferreira, Fernanda Alves, Ewerton Garcia de Oliveira Mima, Janaina Habib Jorge, Ana Cláudia Pavarina Department of Dental Materials and Prosthodontics, Araraquara Dental School, Univ Estadual Paulista – UNESP, Brazil This study evaluated the capacity of biofilm formation of C. albicans recovered from the tongues of murine model of oral candidosis1, after consecutive applications of Photodithazine® (PDZ) (100 mg/L) associated with LED (660 nm) light (37.5 J/cm2 ). Mice with oral candidosis were treated with photodynamic therapy (PDT) and Nystatin (NYS). Next, fungal cells were recovered from the oral lesions and submitted to biofilm formation. The capacity of biofilm formation was analyzed by the cellular viability (CFU/mL), XTT assay and Violet Crystal staining assay (CV). Data were analyzed by ANOVA and Tukey post hoc (p < 0.05). PDT and NYS promoted a significant reduction in the biofilm formation of C. albicans in all tests performed, when compared to the control groups (p < 0.05). The cell viability reduced 1.6 and 2 log 10, for the groups treated with PDT and NYS, respectively. The XTT assay revealed 58% and 62% of reduction in the metabolic activity and the CV staining assay showed 82,4% and 77,5% of reduction in the total biomass, for the groups treated with PDT and NYS respectively. PDZmediated PDT treatment reduced the capacity of biofilm formation
1 Fluminense Federal University – UFF – Niterói – Rio de Janeiro, Brazil 2 São Carlos Institute of Physics, University of São Paulo, Brazil
Actinic Cheilitis (AC) is a common pre-cancerous lesion of the lip vermilion in response to chronic solar exposure. Microscopically, various degrees of epithelial dysplasia (ED) may be encountered. Photodynamic therapy (PDT) has been widely used in Dermatology, but its efficacy for AC is still controversial. The use of CO2 laser before PDT for cutaneous lesions has been shown to allow more uniform distribution of the photosensitizer and to improve its absorption. Our aim was to evaluate the efficacy of one session of PDT for AC with previous application of CO2 laser. Methods: Eight patients with AC of the lower lip were treated with one session of 20% methyl-aminolevulinate cream and red LED light source with previous fractioned ablative CO2 laser application. The outcomes were evaluated after a six-month period. Clinical alterations were mapped and registered before and after treatment. Histopathological outcomes were evaluated based on the presence and grading of ED. Results: All patients showed clinical improvement after PDT. Histopathological analysis showed that 66.6% of patients presented improvement of ED after PDT. Conclusions: One session of PDT with previous CO2 laser application, however further studies are needed in order to confirm this protocol’s efficacy. http://dx.doi.org/10.1016/j.pdpdt.2015.07.056 Photodynamic killing of cancer cells by a platinum-based compound, a derivative of Pt(II) dipyridobenzene Rachel E. Doherty, Igor V. Sazanovich, Luke K. McKenzie, Alexander S. Stasheuski, Rachel Coyle, Elizabeth Baggaley, Sarah Bottomley, Julia A. Weinstein, Helen E. Bryant Academic Unit of Molecular Oncology, Department of Oncology, University of Sheffield, United Kingdom The use of light-activated pro-drugs in cancer treatment is becoming increasingly popular due to their ability to selectively target tumour material whilst remaining non-toxic to healthy tissue. The study describes a novel platinum-based photosensitizer Pt(II) dipyridotolylchloride PtNCNMe. We demonstrate that activation of PtNCNMe (0.1–1 M) by light (405 nm) results in significant cell death of various cancer cell lines (including a cisplatin-resistant cell line). Treatment with neither light nor compound alone led to cell death. Furthermore, it is shown that PtNCNMe induces irreversible DNA single strand breaks following irradiation. In this way, we describe a promising new agent