- Email: [email protected]
Analytical performance of DxN Veris system in the viral load quantification of Hepatitis C virus
S88
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
The ethnobotanical survey in Northern Cyprus illustrated that young leaves of T. capitatus is used to treat mouth ulcers and oral cancers. The aim of this study is to analyse the chemical composition of the essential oils (EOs) of T. capitatus and the antimicrobial activity against Helicobacter pylori (H. pylori). Aerial parts of wild growing plant materials were collected during flowering stage from three different locations; Western (Tc1), Eastern (Tc2), and Northern (Tc3) regions in Northern Cyprus. EO composition analyses were performed simultaneously by GC-FID and GC-MS systems. The antimicrobial activity of the EOs were tested by broth dilution method. Tc3 showed the lowest MIC value and the strongest bactericidal activity against H. pylori. In conclusion, bactericidal efficacy of EOs of T. capitatus on H. pylori could have been affected due to the varied rates of major compounds. Thymus capitatus EO could be an alternative way for the treatment of H. pylori infections. Further investigations are needed to determine the in vivo antimicrobial activity against H. pylori.
Bcl-2, caspase3, etc. Therefore, we examined the targets, which is related proliferation and apoptosis, of MYC family genes in NSCLC by suppression of their activities with siRNAs. To check the effect of Myc expression on cellular proliferation and apoptosis, we designed knockdown MYC expression on cellular proliferation and gene expression profiling were evaluated in MYC overexpressed and knockdown NSLC cells. TBFR I–II expressions were evaluated by western blot, mRNA levels of TBFR I–II, cyclinD1, p21, Bcl-2 by qRT-PCR analysis. MYC inhibition in H1975 cells were found to be increased TBFR I-levels. Besides to, Cyclin-D1 levels and cell proliferation were decreased, we also observed high p21 level and low Bcl-2 level due to MYC inhibition. The results indicate that NSCLC cells are addicted to Myc proteins for their growth and MYC regulates proliferation and apoptosis by targeting TGFRs family. Firstly, the interaction with TGFRs and MYC in NSCLC is indicated in other studies.
http://dx.doi.org/10.1016/j.jbiotec.2017.06.1099
http://dx.doi.org/10.1016/j.jbiotec.2017.06.1101
Investigation of the effect on Rose Bengal dye to “Candida albicans” by XTT colorimetric assay
Analytical performance of DxN Veris system in the viral load quantification of Hepatitis C virus
Meltem Ulusoy ∗ , Irem C¸elebier, Oznur Irem Yayalar, Nevin Keskin
Murat Sayan 1 , Murat Sayan 2 , Tamer Sanlidag 2,∗ , Tamer Sanlidag 3,∗ , Ayse Arikan 4 , Ayse Arikan 5
Department of Biology Hacettepe University, Ankara, Turkey
1
E-mail address: [email protected] (M. Ulusoy). “Candida albicans” can cause infection by forming biofilms. These biofilms are resistant to anti-fungal agents. Rose Bengal is a chemical dye which used for various purposes in the biological fields. This dye is also used invitro for clinical trials such as inhibition of biofilm formation on teeth. The XTT colorimetric assay was used to determine the percentage of viable cells. For yeast culture preparation, 200 l of overnight cultures adjusted to 5 × 105 cells/ml was added to 96-well plate. Following 17 h treatment with different concentrations of Rose Bengal, 100 l from each well was transferred to new plate and 25 l XTT/Menadione was added on them. After an incubation period of 1 h at 37 ◦ C, the absorbance at 490 nm was measured by using a microplate reader. The highest two Rose Bengal concentrations (2 mg/ml and 4 mg/ml) were found to be effective on “Candida albicans”. Furthermore, it was identified that there is a correlation between the concentration of Rose Bengal and inhibition of C. albicans. Studies showed that Rose Bengal is a clinically significant substance on “C. albicans”. Consequently, this study is promising for further clinical studies and Rose Bengal may be used as a potential drug against “C. albicans” biofilms future. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1100 The expression of MYC regulates cellular proliferation and apoptosis through TGF-beta receptor dependent manner Ege Riza Karagur ∗ , Onur Tokgun, Hakan Akca Department of Medical Biology Pamukkale University, Denizli, Turkey
Kocaeli University, Faculty of Medicine, Clinical Laboratory, PCR Unit, Kocaeli, Turkey 2 Near East University, Research Center of Experimental Health Sciences, Nicosia, Cyprus 3 Celal Bayar University, Faculty of Medicine, Department of Medical Microbiology, Manisa, Turkey 4 Near East University, Faculty of Medicine, Department of Medical Microbiology, Nicosia, Cyprus 5 Kyrenia University, Faculty of Medicine, Department of Medical Microbiology, Kyrenia, Cyprus E-mail address: [email protected] (T. Sanlidag). DxN VERIS System is a new automated, random access molecular system formed by combining reverse transcription and real-time polymerase chain reaction (RT-PCR) for quantifying hepatitis C virus (HCV) RNA. This study aimed to evaluate DxN Veris HCV (Beckman Coulter DxN Veris HCV kit, U.S.A) and Qiagen HCV assays (Artus HCV Qiagen kit, Germany) performance in clinical samples. Qiagen HCV RNA results of 44 samples with the HCV RNA levels ranging from negative to >106 IU/ml from Kocaeli provience were compared with Veris DxN HCV results. Bland Altman Data Plotting and Passing-Bablok regression analysis methods were used to analyze assays. According to the Bland Altman Plot (Veris combined Qiagen Log IU/ml), the specificity resulted 95% LoA (Cl 95% = −1.59–0.72), correlation 0.90 with standard deviation (SD) 0.59 and a mean SD = −0.43. HCV Passing-Bablok regression analysis shows that Veris combined = −0.3394 + 0.8802 Qiagen Log IU/ml with correlation 0.90. Based on these data, the VERIS HCV assay can be accepted as a rapid, automated molecular test for the sensitive, repeatable, and accurate viral load monitoring required for the detection of virus reactivation and disease as well as for the management of patient therapy.
E-mail address: [email protected] (E.R. Karagur). Non-small cell lung cancer (NSCLC) cell line H1975 has a highly MYC amplification. Myc proto-oncogenes plays a key role in the regulation of cell proliferation, growth, differentiation and apoptosis. The role of c-Myc in proliferation and apoptosis control varies depending on the cellular context such as; TGFR, cyclin-D1, p21,
http://dx.doi.org/10.1016/j.jbiotec.2017.06.1102
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
The ethnobotanical survey in Northern Cyprus illustrated that young leaves of T. capitatus is used to treat mouth ulcers and oral cancers. The aim of this study is to analyse the chemical composition of the essential oils (EOs) of T. capitatus and the antimicrobial activity against Helicobacter pylori (H. pylori). Aerial parts of wild growing plant materials were collected during flowering stage from three different locations; Western (Tc1), Eastern (Tc2), and Northern (Tc3) regions in Northern Cyprus. EO composition analyses were performed simultaneously by GC-FID and GC-MS systems. The antimicrobial activity of the EOs were tested by broth dilution method. Tc3 showed the lowest MIC value and the strongest bactericidal activity against H. pylori. In conclusion, bactericidal efficacy of EOs of T. capitatus on H. pylori could have been affected due to the varied rates of major compounds. Thymus capitatus EO could be an alternative way for the treatment of H. pylori infections. Further investigations are needed to determine the in vivo antimicrobial activity against H. pylori.
Bcl-2, caspase3, etc. Therefore, we examined the targets, which is related proliferation and apoptosis, of MYC family genes in NSCLC by suppression of their activities with siRNAs. To check the effect of Myc expression on cellular proliferation and apoptosis, we designed knockdown MYC expression on cellular proliferation and gene expression profiling were evaluated in MYC overexpressed and knockdown NSLC cells. TBFR I–II expressions were evaluated by western blot, mRNA levels of TBFR I–II, cyclinD1, p21, Bcl-2 by qRT-PCR analysis. MYC inhibition in H1975 cells were found to be increased TBFR I-levels. Besides to, Cyclin-D1 levels and cell proliferation were decreased, we also observed high p21 level and low Bcl-2 level due to MYC inhibition. The results indicate that NSCLC cells are addicted to Myc proteins for their growth and MYC regulates proliferation and apoptosis by targeting TGFRs family. Firstly, the interaction with TGFRs and MYC in NSCLC is indicated in other studies.
http://dx.doi.org/10.1016/j.jbiotec.2017.06.1099
http://dx.doi.org/10.1016/j.jbiotec.2017.06.1101
Investigation of the effect on Rose Bengal dye to “Candida albicans” by XTT colorimetric assay
Analytical performance of DxN Veris system in the viral load quantification of Hepatitis C virus
Meltem Ulusoy ∗ , Irem C¸elebier, Oznur Irem Yayalar, Nevin Keskin
Murat Sayan 1 , Murat Sayan 2 , Tamer Sanlidag 2,∗ , Tamer Sanlidag 3,∗ , Ayse Arikan 4 , Ayse Arikan 5
Department of Biology Hacettepe University, Ankara, Turkey
1
E-mail address: [email protected] (M. Ulusoy). “Candida albicans” can cause infection by forming biofilms. These biofilms are resistant to anti-fungal agents. Rose Bengal is a chemical dye which used for various purposes in the biological fields. This dye is also used invitro for clinical trials such as inhibition of biofilm formation on teeth. The XTT colorimetric assay was used to determine the percentage of viable cells. For yeast culture preparation, 200 l of overnight cultures adjusted to 5 × 105 cells/ml was added to 96-well plate. Following 17 h treatment with different concentrations of Rose Bengal, 100 l from each well was transferred to new plate and 25 l XTT/Menadione was added on them. After an incubation period of 1 h at 37 ◦ C, the absorbance at 490 nm was measured by using a microplate reader. The highest two Rose Bengal concentrations (2 mg/ml and 4 mg/ml) were found to be effective on “Candida albicans”. Furthermore, it was identified that there is a correlation between the concentration of Rose Bengal and inhibition of C. albicans. Studies showed that Rose Bengal is a clinically significant substance on “C. albicans”. Consequently, this study is promising for further clinical studies and Rose Bengal may be used as a potential drug against “C. albicans” biofilms future. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1100 The expression of MYC regulates cellular proliferation and apoptosis through TGF-beta receptor dependent manner Ege Riza Karagur ∗ , Onur Tokgun, Hakan Akca Department of Medical Biology Pamukkale University, Denizli, Turkey
Kocaeli University, Faculty of Medicine, Clinical Laboratory, PCR Unit, Kocaeli, Turkey 2 Near East University, Research Center of Experimental Health Sciences, Nicosia, Cyprus 3 Celal Bayar University, Faculty of Medicine, Department of Medical Microbiology, Manisa, Turkey 4 Near East University, Faculty of Medicine, Department of Medical Microbiology, Nicosia, Cyprus 5 Kyrenia University, Faculty of Medicine, Department of Medical Microbiology, Kyrenia, Cyprus E-mail address: [email protected] (T. Sanlidag). DxN VERIS System is a new automated, random access molecular system formed by combining reverse transcription and real-time polymerase chain reaction (RT-PCR) for quantifying hepatitis C virus (HCV) RNA. This study aimed to evaluate DxN Veris HCV (Beckman Coulter DxN Veris HCV kit, U.S.A) and Qiagen HCV assays (Artus HCV Qiagen kit, Germany) performance in clinical samples. Qiagen HCV RNA results of 44 samples with the HCV RNA levels ranging from negative to >106 IU/ml from Kocaeli provience were compared with Veris DxN HCV results. Bland Altman Data Plotting and Passing-Bablok regression analysis methods were used to analyze assays. According to the Bland Altman Plot (Veris combined Qiagen Log IU/ml), the specificity resulted 95% LoA (Cl 95% = −1.59–0.72), correlation 0.90 with standard deviation (SD) 0.59 and a mean SD = −0.43. HCV Passing-Bablok regression analysis shows that Veris combined = −0.3394 + 0.8802 Qiagen Log IU/ml with correlation 0.90. Based on these data, the VERIS HCV assay can be accepted as a rapid, automated molecular test for the sensitive, repeatable, and accurate viral load monitoring required for the detection of virus reactivation and disease as well as for the management of patient therapy.
E-mail address: [email protected] (E.R. Karagur). Non-small cell lung cancer (NSCLC) cell line H1975 has a highly MYC amplification. Myc proto-oncogenes plays a key role in the regulation of cell proliferation, growth, differentiation and apoptosis. The role of c-Myc in proliferation and apoptosis control varies depending on the cellular context such as; TGFR, cyclin-D1, p21,
http://dx.doi.org/10.1016/j.jbiotec.2017.06.1102