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Androgen-deprivation therapy-induced atherosclerosis in prostate cancer: Differences between GnRH-agonist and antagonist
Abstracts / Atherosclerosis 252 (2016) e1ee196
Child Pugh and Meld scores. Hyponatremia prevalence and mortality were calculated. Statistical analysis was performed using SPSS 20.0. Results: We identified 78 patients with liver cirrhosis; sex-ratio¼1.6. Hepatitis C was found in 45% of patients. Child Pugh class was A in 14%, B in 50% and C in 36% of the subjects. Among all patients, 35% had serum sodium value between 130-135mmol/L and 17% less than 130mmol/L. The mortality among those cirrhotic patients was 19%. This mortality was higher in patients with hyponatremia<130 mmol/L than in those with hyponatremia between 130-135mmol/L. Conclusions: Hyponatremia is commun in hospitalized patients and is a predictor of poor prognosis. A more careful clinical concern of hyponatremia during hospitalization is necessary to attenuate the increased mortality risk.
EAS16-1035, GENETICS, NUTRITION, BIOMARKERS: GUIDELINES AND MISCELLANEOUS. ANDROGEN-DEPRIVATION THERAPY-INDUCED ATHEROSCLEROSIS IN PROSTATE CANCER: DIFFERENCES BETWEEN GNRH-AGONIST AND ANTAGONIST W. Duivenvoorden 1, S. Hopmans 1, G. Werstuck 2, J. Pinthus 1. 1 McMaster University, Surgery, Hamilton, Canada; 2 McMaster University, Medicine, Hamilton, Canada Objectives: Cardiovascular disease (CVD) is one of the most common causes of mortality in prostate cancer patients. In prostate cancer treatment androgen deprivation therapy (ADT) is associated with weight gain and development of the metabolic syndrome. However, different modes of ADT can achieve castration. A post-hoc analysis of phase III trials suggests that GnRH-antagonists are associated with less cardiac events in men with pre-existing CVD during the first year of ADT. We investigated the effects of GnRH-agonist and GnRH-antagonist on the development of CVD in two mouse models. Methods: Ins2+/Akita:apoE-/- who succumb to CVD and LDLR-/- mice (n¼10/ group) were used to compare the effects of GnRH-antagonist (degarelix) and GnRH-agonist (leuprolide). Longitudinal weight gain (4 months), visceral fat accumulation (CT measurements), fasting glucose tolerance, blood pressure, and testosterone levels were studied along with characteristics of aortic atherosclerotic plaques, and survival. Results: Leuprolide-treated LDLR-/- mice gained significantly more weight and visceral fat compared to mice treated with degarelix. Significantly better glucose tolerance was recorded in mice treated with degarelix. The aortic atherosclerotic plaque area in leuprolide-treated mice was significantly larger than in control mice, but not significantly different from control after degarelix-treatment. The necrotic plaque area in degarelixtreated mice was significantly smaller than in leuprolide-treated mice. Kaplan-Meier plots show that leuprolide-treatment of Ins2+/Akita:apoE-/mice led to significantly shorter median survival compared with degarelixtreatment (31.1 vs 39.7 weeks, p¼0.022). Conclusions: In preclinical mouse models, the use of GnRH-antagonist attenuates development of atherosclerosis and is associated with increased survival.
EAS16-0750, GENETICS, NUTRITION, BIOMARKERS: GUIDELINES AND MISCELLANEOUS. RELIABILITY ASSESSMENT OF DETERMINATION OF PROCALCITONIN IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS : ABOUT TWO CASES A. Bartkiz 1, K. Bouzid 1, 2, S. Ghezaiel 1, S. Lahiani 1, S. Hammami 1, E. A. Zannouri 3, M. Omrane 3, S. Barbouche 3, J. Talbi 1, ^pital Charles Nicolle, Biochemistry, Tunis, Tunisia; Abdelmoula 1. 1 Ho 2 ^ Hopital Charles Nicolle, Laboratory of Engineering of proteins and ^pital bioactive molecules INSAT: LR 11EES24:LIP-MB, Tunis, Tunisia; 3 Ho Charles Nicolle, Nephrology, Tunis, Tunisia
e87
Objectives: The purpose of the study was to evaluate the reliability of the procalcitonin (PCT) as a marker of bacterial infection in patients with systemic lupus erythematosus (SLE). Methods: We report the cases of two patients hospitalized in nephrology. The first one was 24 years old; suffering from SLE stage chronic renal failure admitted for suspected lupus flare in presence of fever and pancytopenia. The patient was put under antibiotic therapy at admission. The second patient was 31 years old, was known suffering from SLE and was admitted for right femoral thrombosis complicated by severe sepsis treated with triple antibiotic. For the two patients, over the lack of infectious focus and the lack of decline in CRP, a PCT assay was requested. Results: The kinetic of PCT in the first patient fell from 47.4 ng/ml for admission to 38.39 ng/ml on day 7 and then 68.38 ng/ml on day 14. In the absence of decline in the PCT and no obvious focus of infection antibiotic therapy was discontinued and a therapeutic window has been started. The assay was done again on day 21 (PCT¼24ng/ml). For the second patient, the PCT was>200 ng/ml and this despite the absence of possible infection and triple antibiotic therapy. Conclusions: The case of the two lupus patients let us to ask about the reliability of PCT as a marker of bacterial infection, and prove no correlation between PCT levels and the severity of the infection. It would be more sensible to take greater account the kinetic of the PCT.
EAS16-0033, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. ABNORMAL BALGHAM MUNZIQ, A UIGHUR HERBAL MEDICINE FORMULA, HAS BEEN RECENTLY SHOWN TO POSSESS EXTENSIVE CARDIOVASCULAR PHARMACOLOGICAL ACTIVITIES D. Xilifu 1, A. Nurmuhammat 2, U. Halmurat 2. 1 the first affliated hospital of xinjiang medical university, Care unit of Cardiology center, Urumqi, China; 2 Xinjiang Medical University, Traditional Uighur Medicine Institute, Urumqi, China Objectives: Atherosclerosis (AS) is a state of heightened oxidative stress characterized by lipid and protein oxidation in vascular walls. Inflammation of vascular smooth muscle cells (VSMC) is intimately linked to atherosclerosis and other vascular inflammatory disease. Abnormal Balgham Munziq, a Uighur herbal medicine formula, has been widely used in traditional Uighur medicine for a long time, has been recently shown to possess extensive cardiovascular pharmacological activities. Methods: The in vivo study using an ApoE/mouse model of AS demonstrated that Abnormal Balgham Munziq treatment protects against VEC damage and inhibits early AS plaque formation. Results: Meanwhile,though the serum lipid levels in ApoE/ mice were markedly higher than those in C57BL/6N mice, significant differences were observed between the Abnormal Balgham Munziq and model groups in ApoE/ mice, suggesting that the Abnormal Balgham Munziq effect on inhibiting AS was associated with reducing blood lipids. Abnormal Balgham Munziq for 2 months can remarkably reduce the serum levels of MCP1, CRP and sICAM-1 compared to vehicle treated ApoE/ model group. Conclusions: Abnormal Balgham Munziq markedly decreased the MDA, and increased the activity of antioxidant enzymes of SOD, CAT and GSHPx.This effect is associated with the antioxidative and anti-iflammatory effect of Abnormal Balgham Munziq.
EAS16-1003, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. EFFECTS OF RAMADAN FASTING ON BIOLOGICAL PARAMETERS IN PATIENTS WITH CARDIOVASCULAR RISK FACTORS K. Bouzid 1, W. Hmaidi 2, Y. Molahedh 2, H. Ben Ahmed 3, A. Bartkiz 2, H. Baccar 3, J. Abdelmoula 2. 1 hopital charles nicolle, biochemistry-Laboratory of Engineering of proteins and bioactive molecules INSAT: LR 11EES24:LIPMB, Tunis, Tunisia; 2 hopital charles nicolle, biochemistry, Tunis, Tunisia; 3 hopital charles nicolle, Cardiology, Tunis, Tunisia Objectives: Fasting during Ramadan is a religious ritual of all healthy adult Muslims. However, there is no clear agreement on the effects of Ramadan
Child Pugh and Meld scores. Hyponatremia prevalence and mortality were calculated. Statistical analysis was performed using SPSS 20.0. Results: We identified 78 patients with liver cirrhosis; sex-ratio¼1.6. Hepatitis C was found in 45% of patients. Child Pugh class was A in 14%, B in 50% and C in 36% of the subjects. Among all patients, 35% had serum sodium value between 130-135mmol/L and 17% less than 130mmol/L. The mortality among those cirrhotic patients was 19%. This mortality was higher in patients with hyponatremia<130 mmol/L than in those with hyponatremia between 130-135mmol/L. Conclusions: Hyponatremia is commun in hospitalized patients and is a predictor of poor prognosis. A more careful clinical concern of hyponatremia during hospitalization is necessary to attenuate the increased mortality risk.
EAS16-1035, GENETICS, NUTRITION, BIOMARKERS: GUIDELINES AND MISCELLANEOUS. ANDROGEN-DEPRIVATION THERAPY-INDUCED ATHEROSCLEROSIS IN PROSTATE CANCER: DIFFERENCES BETWEEN GNRH-AGONIST AND ANTAGONIST W. Duivenvoorden 1, S. Hopmans 1, G. Werstuck 2, J. Pinthus 1. 1 McMaster University, Surgery, Hamilton, Canada; 2 McMaster University, Medicine, Hamilton, Canada Objectives: Cardiovascular disease (CVD) is one of the most common causes of mortality in prostate cancer patients. In prostate cancer treatment androgen deprivation therapy (ADT) is associated with weight gain and development of the metabolic syndrome. However, different modes of ADT can achieve castration. A post-hoc analysis of phase III trials suggests that GnRH-antagonists are associated with less cardiac events in men with pre-existing CVD during the first year of ADT. We investigated the effects of GnRH-agonist and GnRH-antagonist on the development of CVD in two mouse models. Methods: Ins2+/Akita:apoE-/- who succumb to CVD and LDLR-/- mice (n¼10/ group) were used to compare the effects of GnRH-antagonist (degarelix) and GnRH-agonist (leuprolide). Longitudinal weight gain (4 months), visceral fat accumulation (CT measurements), fasting glucose tolerance, blood pressure, and testosterone levels were studied along with characteristics of aortic atherosclerotic plaques, and survival. Results: Leuprolide-treated LDLR-/- mice gained significantly more weight and visceral fat compared to mice treated with degarelix. Significantly better glucose tolerance was recorded in mice treated with degarelix. The aortic atherosclerotic plaque area in leuprolide-treated mice was significantly larger than in control mice, but not significantly different from control after degarelix-treatment. The necrotic plaque area in degarelixtreated mice was significantly smaller than in leuprolide-treated mice. Kaplan-Meier plots show that leuprolide-treatment of Ins2+/Akita:apoE-/mice led to significantly shorter median survival compared with degarelixtreatment (31.1 vs 39.7 weeks, p¼0.022). Conclusions: In preclinical mouse models, the use of GnRH-antagonist attenuates development of atherosclerosis and is associated with increased survival.
EAS16-0750, GENETICS, NUTRITION, BIOMARKERS: GUIDELINES AND MISCELLANEOUS. RELIABILITY ASSESSMENT OF DETERMINATION OF PROCALCITONIN IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS : ABOUT TWO CASES A. Bartkiz 1, K. Bouzid 1, 2, S. Ghezaiel 1, S. Lahiani 1, S. Hammami 1, E. A. Zannouri 3, M. Omrane 3, S. Barbouche 3, J. Talbi 1, ^pital Charles Nicolle, Biochemistry, Tunis, Tunisia; Abdelmoula 1. 1 Ho 2 ^ Hopital Charles Nicolle, Laboratory of Engineering of proteins and ^pital bioactive molecules INSAT: LR 11EES24:LIP-MB, Tunis, Tunisia; 3 Ho Charles Nicolle, Nephrology, Tunis, Tunisia
e87
Objectives: The purpose of the study was to evaluate the reliability of the procalcitonin (PCT) as a marker of bacterial infection in patients with systemic lupus erythematosus (SLE). Methods: We report the cases of two patients hospitalized in nephrology. The first one was 24 years old; suffering from SLE stage chronic renal failure admitted for suspected lupus flare in presence of fever and pancytopenia. The patient was put under antibiotic therapy at admission. The second patient was 31 years old, was known suffering from SLE and was admitted for right femoral thrombosis complicated by severe sepsis treated with triple antibiotic. For the two patients, over the lack of infectious focus and the lack of decline in CRP, a PCT assay was requested. Results: The kinetic of PCT in the first patient fell from 47.4 ng/ml for admission to 38.39 ng/ml on day 7 and then 68.38 ng/ml on day 14. In the absence of decline in the PCT and no obvious focus of infection antibiotic therapy was discontinued and a therapeutic window has been started. The assay was done again on day 21 (PCT¼24ng/ml). For the second patient, the PCT was>200 ng/ml and this despite the absence of possible infection and triple antibiotic therapy. Conclusions: The case of the two lupus patients let us to ask about the reliability of PCT as a marker of bacterial infection, and prove no correlation between PCT levels and the severity of the infection. It would be more sensible to take greater account the kinetic of the PCT.
EAS16-0033, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. ABNORMAL BALGHAM MUNZIQ, A UIGHUR HERBAL MEDICINE FORMULA, HAS BEEN RECENTLY SHOWN TO POSSESS EXTENSIVE CARDIOVASCULAR PHARMACOLOGICAL ACTIVITIES D. Xilifu 1, A. Nurmuhammat 2, U. Halmurat 2. 1 the first affliated hospital of xinjiang medical university, Care unit of Cardiology center, Urumqi, China; 2 Xinjiang Medical University, Traditional Uighur Medicine Institute, Urumqi, China Objectives: Atherosclerosis (AS) is a state of heightened oxidative stress characterized by lipid and protein oxidation in vascular walls. Inflammation of vascular smooth muscle cells (VSMC) is intimately linked to atherosclerosis and other vascular inflammatory disease. Abnormal Balgham Munziq, a Uighur herbal medicine formula, has been widely used in traditional Uighur medicine for a long time, has been recently shown to possess extensive cardiovascular pharmacological activities. Methods: The in vivo study using an ApoE/mouse model of AS demonstrated that Abnormal Balgham Munziq treatment protects against VEC damage and inhibits early AS plaque formation. Results: Meanwhile,though the serum lipid levels in ApoE/ mice were markedly higher than those in C57BL/6N mice, significant differences were observed between the Abnormal Balgham Munziq and model groups in ApoE/ mice, suggesting that the Abnormal Balgham Munziq effect on inhibiting AS was associated with reducing blood lipids. Abnormal Balgham Munziq for 2 months can remarkably reduce the serum levels of MCP1, CRP and sICAM-1 compared to vehicle treated ApoE/ model group. Conclusions: Abnormal Balgham Munziq markedly decreased the MDA, and increased the activity of antioxidant enzymes of SOD, CAT and GSHPx.This effect is associated with the antioxidative and anti-iflammatory effect of Abnormal Balgham Munziq.
EAS16-1003, GENETICS, NUTRITION, BIOMARKERS: NUTRITION. EFFECTS OF RAMADAN FASTING ON BIOLOGICAL PARAMETERS IN PATIENTS WITH CARDIOVASCULAR RISK FACTORS K. Bouzid 1, W. Hmaidi 2, Y. Molahedh 2, H. Ben Ahmed 3, A. Bartkiz 2, H. Baccar 3, J. Abdelmoula 2. 1 hopital charles nicolle, biochemistry-Laboratory of Engineering of proteins and bioactive molecules INSAT: LR 11EES24:LIPMB, Tunis, Tunisia; 2 hopital charles nicolle, biochemistry, Tunis, Tunisia; 3 hopital charles nicolle, Cardiology, Tunis, Tunisia Objectives: Fasting during Ramadan is a religious ritual of all healthy adult Muslims. However, there is no clear agreement on the effects of Ramadan