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Anti interleukin-1 alpha autoantibodies in sera of scleroderma patients, but not MCTD nor dermatomyositis patients
E8
E7 Biochemical and functional characteristics of class II MHC antigen (la) on freshlyprepared Langerhans
cells (fLC) and cultured Langerhans
cells (cLC)
Setsuya Alba and Stephen I. Katz Department of Dermatology, Tohoku University School of Medicine. Dermatology
Branch
Sendai
NCI. USA
MSINOPIUIS CONTAIN IN HUMAN -TIN-
THE FACIORS WHICH (KC) CELL LINE
VPREGVIATE
IL-6 ,nRNA
.-IIDEKAZUYAMADA, ToMoAK ORITA, YOSHINORI ARAGANE, *JVNICXI CHIHAR&,TADASHI TEZVKA Departmeiit of Cermatolcqy, *Internal Medxlne, KInki
Univ.
Osak;
on cLC is poor, but that the complex of peptide and la antigen, once formed, are very stable on the suriace of cLC
Eoslnophlls are well known cells to partlclpate with the allerqlc reaction of the skin, especially of atopx dermatitis. Its bIologica effects are thought to br due to Its spzclfuz protons, major basic protein(MBP), eoslnophll pt:roxldase(&Q) eoslnophllic cationIc protein(ECP). In order to investigate how do eosinophils patlcipate I" the allerqlc reaction of the may skin, we focused, this time, to examxne If mslnophlls enhance the IL-6 production of KC. Fmslnophlls were collected from tha peripheral blood of h~reosinouhil~c sandrome mtient and were purified up to 90% bye&&r~zam& solution. Crude extracts were added to the normal human KC, KC cell 1~~s and the amount of IL-6 mP.NA was detected by Northern hybrldlzation techniwe. The results showed that crude eoslnophrl extract induced the IL-6 mRNA ezression I” KC cell llnez,. The factor which enhanced the IL-6 mRNA expression is now under xwestlqatio".
E9
El0
CELL SURFACE PATTERN OF ICAMAND HLA-DR ON EPIDERMAL BASAL CELLS OF MYCOSIS FUNGOIDES
SERLihf
CYTOKINE
PUSTUI.
4R
We examined the synthesis and new evpresslon of la by fLC and cLC in ihe folIowIng ways. (11 the blocklng study using cycloheximlde and Brefeldln A, (2) the mvnunoprec~pltation of la antigen. (3) the study using the antlbody iM5,114 5 2) which can block the bindmg of anti-i-A antibody (MKDG) to I-A In either way. fLC are far more actwe in blosynthesls and surface expression of la anilgen than cLC Interestingly, however. these cLC do not lose surface la antigen slgnlflcantly even after 10 days culture. Functionally, LC which are pulsed with antigen Immediately after obtained from the skin can keep antngen presenting function after 10 days culture In the presence of different concentrations of antigenlc peptlde. cLC are less efficient than fLC in the stlmulatlon of T cell clone These data suggest that surface la on cLC are metabolically stable. and that functionally. the association of peptide lo la antigen
SHUHEI IMAYAMA, YUTAKA YASHIMA, MINAO FURUMURA , YOSHIAKI HORI Department of Dermatology, Kyushu University, Fukuoka A combination of epidenoal separation from dermal connective tissue and immune-gold labeling was applied to demonstrate the surface distribution of ICAM- 1, together with LFA- 1,and HLA-DR expressed by the epidermal basal cells utilizing scanning electron microscopy(SEM), in an attempt to demonstrate a possible correlation behveen the distribution pattern of those molecules on the epidrmal basal surface and cell migration pattern to the epidermis in mycosis fimgoides. ICAM-I, expressed on the intercellular processes of keratinocytes, may allow LFA-I bearing lymphocytes migrate into the epidermal interspaces, thus producing “epidetmotropism”. Surface HLA-DR expressed on the border of individual basal cells, similar to that of normal eccrine duct, may be of significance in avoiding the lymphocyte attack against the epidermis. which also characterizes the histology of mycosis fungoides.
LEVELS
ALPHA AVTOANTIBODIES IN SERA 0 I‘ SCLERODERMA PATIENTS, BUT NOR NOT MCTD DERMATOMYOSITIS PATIENTS HTTOSHI MIZUTANI, TOMOKO MIZVTANI, MINORU MURATA, YASUKAZL‘ OHMOTO* AND MASAYUKI SHIMIZU Department of Dermatology, Mie Vnlv. School of Medlclile, TS", Otsuka Cellular Technology Instltaltr, Tok"shlma* Recent ~"vestlgatlons declared the existence of Ii.-1 antagonlsts 1" body fluIds. The other hand, s,~me ailtolmmune diseases generate autoantlbodles aqainst the essent1a1 CL?11 components. We measured autoantIbodIes against rh-IL-1 alpha and brta 1" serum from progress1"fZ systemic scleros1s(PSS) and related diseases using radio-lmmuno assay. we found anti IL-1 alpha ar,tlboay I" 6/29(21$) of PSS, l/4(25%) of morphea ar.cl ?/2(100%) of eosinophllia myalgia syndrome. HoWeVer none of 5 MCTD, 7 dermatomyosistls and 20 norma, control serum had ant1 IL-1 alpha antlbrid1es. Ant1 IL-1 beta antibody was negative 1r i(i 1 samples. IL-1 alpha can be one of the pLtatzve pathognomotlc factors in PSS.
GENERAI.I%ED
AKIHIKO SHIBAKI, HITOSHI KORAYASHI, AKIRA OHKAWARA. TOMONORI MINAGAWA Department of Dermatology and Microbiology. Hokkaido School of Medicine. Sapporo Generalized
pustular
of psoriasis malaise,
with
arthralgia
that cytokines the serum In all
five
Though
was elevated cytokine results
GPP
lrvels swxest
TNF
cases
of i
TNFmn
the- sever
forms
Since it has been suggested I
by winy
GPP
was observed that
IFN-
TNF-
was elevated
cases.
of
lfniv.
such as kiuh fever,
to the pathwenesis
a.
patients
IFNin four
) is me
symptoms
and leukocytosis.
levels of
of
(GPP
systemic
could contribute
five cases of stage.
psoriasis
serious
involved in the pathoRenesis
El1 ANTI INTERLEUKIN-1
IN
PSORIASIS
of psoriasis,
and IL
6 wemstudied
ELISA
and
in
RIA.
a was el?vat?d :at pustular in rhrre cases and
no simificant
elevation
IL-
vulgaris.
These
and other cytokines
may br
in psoriasis and systemic
symptoms
6
of serum
cof GPP.
El2 ANTI-CARBONIC ANHYDRASE ANTIBODY -ITS RIOLOGICAL AND PATHOPHYSIOL@GICAI.
NATL%E
YASUNORI LNAGAKI, YOKO YOSHIDA, ?IARIKO KAN%AhI, YOUICHIROU HAMASAEI AND HIROAKI UEKI Department of Dermatology, Kawasaki Medical School,
Okayama
A novel autoantibody reactive with carbonic anhvdraseICA) has been found in sera from patients several with systemic autoimmune diseases, including SLE, SiGrren’s svndrome. Progressive Sysremic Sclerosis,-Dermatomvosltis, . ElCTA and so on. The autoantibody varied in the enrent of its crossreactivity among human CA I, human CA II, bovine CA II. rabbit CA, and dog CA. The titres continued to float and tended to parallel disease activity. Furthermore, the autoantibody may reduced the enzymic activitv.
E7 Biochemical and functional characteristics of class II MHC antigen (la) on freshlyprepared Langerhans
cells (fLC) and cultured Langerhans
cells (cLC)
Setsuya Alba and Stephen I. Katz Department of Dermatology, Tohoku University School of Medicine. Dermatology
Branch
Sendai
NCI. USA
MSINOPIUIS CONTAIN IN HUMAN -TIN-
THE FACIORS WHICH (KC) CELL LINE
VPREGVIATE
IL-6 ,nRNA
.-IIDEKAZUYAMADA, ToMoAK ORITA, YOSHINORI ARAGANE, *JVNICXI CHIHAR&,TADASHI TEZVKA Departmeiit of Cermatolcqy, *Internal Medxlne, KInki
Univ.
Osak;
on cLC is poor, but that the complex of peptide and la antigen, once formed, are very stable on the suriace of cLC
Eoslnophlls are well known cells to partlclpate with the allerqlc reaction of the skin, especially of atopx dermatitis. Its bIologica effects are thought to br due to Its spzclfuz protons, major basic protein(MBP), eoslnophll pt:roxldase(&Q) eoslnophllic cationIc protein(ECP). In order to investigate how do eosinophils patlcipate I" the allerqlc reaction of the may skin, we focused, this time, to examxne If mslnophlls enhance the IL-6 production of KC. Fmslnophlls were collected from tha peripheral blood of h~reosinouhil~c sandrome mtient and were purified up to 90% bye&&r~zam& solution. Crude extracts were added to the normal human KC, KC cell 1~~s and the amount of IL-6 mP.NA was detected by Northern hybrldlzation techniwe. The results showed that crude eoslnophrl extract induced the IL-6 mRNA ezression I” KC cell llnez,. The factor which enhanced the IL-6 mRNA expression is now under xwestlqatio".
E9
El0
CELL SURFACE PATTERN OF ICAMAND HLA-DR ON EPIDERMAL BASAL CELLS OF MYCOSIS FUNGOIDES
SERLihf
CYTOKINE
PUSTUI.
4R
We examined the synthesis and new evpresslon of la by fLC and cLC in ihe folIowIng ways. (11 the blocklng study using cycloheximlde and Brefeldln A, (2) the mvnunoprec~pltation of la antigen. (3) the study using the antlbody iM5,114 5 2) which can block the bindmg of anti-i-A antibody (MKDG) to I-A In either way. fLC are far more actwe in blosynthesls and surface expression of la anilgen than cLC Interestingly, however. these cLC do not lose surface la antigen slgnlflcantly even after 10 days culture. Functionally, LC which are pulsed with antigen Immediately after obtained from the skin can keep antngen presenting function after 10 days culture In the presence of different concentrations of antigenlc peptlde. cLC are less efficient than fLC in the stlmulatlon of T cell clone These data suggest that surface la on cLC are metabolically stable. and that functionally. the association of peptide lo la antigen
SHUHEI IMAYAMA, YUTAKA YASHIMA, MINAO FURUMURA , YOSHIAKI HORI Department of Dermatology, Kyushu University, Fukuoka A combination of epidenoal separation from dermal connective tissue and immune-gold labeling was applied to demonstrate the surface distribution of ICAM- 1, together with LFA- 1,and HLA-DR expressed by the epidermal basal cells utilizing scanning electron microscopy(SEM), in an attempt to demonstrate a possible correlation behveen the distribution pattern of those molecules on the epidrmal basal surface and cell migration pattern to the epidermis in mycosis fimgoides. ICAM-I, expressed on the intercellular processes of keratinocytes, may allow LFA-I bearing lymphocytes migrate into the epidermal interspaces, thus producing “epidetmotropism”. Surface HLA-DR expressed on the border of individual basal cells, similar to that of normal eccrine duct, may be of significance in avoiding the lymphocyte attack against the epidermis. which also characterizes the histology of mycosis fungoides.
LEVELS
ALPHA AVTOANTIBODIES IN SERA 0 I‘ SCLERODERMA PATIENTS, BUT NOR NOT MCTD DERMATOMYOSITIS PATIENTS HTTOSHI MIZUTANI, TOMOKO MIZVTANI, MINORU MURATA, YASUKAZL‘ OHMOTO* AND MASAYUKI SHIMIZU Department of Dermatology, Mie Vnlv. School of Medlclile, TS", Otsuka Cellular Technology Instltaltr, Tok"shlma* Recent ~"vestlgatlons declared the existence of Ii.-1 antagonlsts 1" body fluIds. The other hand, s,~me ailtolmmune diseases generate autoantlbodles aqainst the essent1a1 CL?11 components. We measured autoantIbodIes against rh-IL-1 alpha and brta 1" serum from progress1"fZ systemic scleros1s(PSS) and related diseases using radio-lmmuno assay. we found anti IL-1 alpha ar,tlboay I" 6/29(21$) of PSS, l/4(25%) of morphea ar.cl ?/2(100%) of eosinophllia myalgia syndrome. HoWeVer none of 5 MCTD, 7 dermatomyosistls and 20 norma, control serum had ant1 IL-1 alpha antlbrid1es. Ant1 IL-1 beta antibody was negative 1r i(i 1 samples. IL-1 alpha can be one of the pLtatzve pathognomotlc factors in PSS.
GENERAI.I%ED
AKIHIKO SHIBAKI, HITOSHI KORAYASHI, AKIRA OHKAWARA. TOMONORI MINAGAWA Department of Dermatology and Microbiology. Hokkaido School of Medicine. Sapporo Generalized
pustular
of psoriasis malaise,
with
arthralgia
that cytokines the serum In all
five
Though
was elevated cytokine results
GPP
lrvels swxest
TNF
cases
of i
TNFmn
the- sever
forms
Since it has been suggested I
by winy
GPP
was observed that
IFN-
TNF-
was elevated
cases.
of
lfniv.
such as kiuh fever,
to the pathwenesis
a.
patients
IFNin four
) is me
symptoms
and leukocytosis.
levels of
of
(GPP
systemic
could contribute
five cases of stage.
psoriasis
serious
involved in the pathoRenesis
El1 ANTI INTERLEUKIN-1
IN
PSORIASIS
of psoriasis,
and IL
6 wemstudied
ELISA
and
in
RIA.
a was el?vat?d :at pustular in rhrre cases and
no simificant
elevation
IL-
vulgaris.
These
and other cytokines
may br
in psoriasis and systemic
symptoms
6
of serum
cof GPP.
El2 ANTI-CARBONIC ANHYDRASE ANTIBODY -ITS RIOLOGICAL AND PATHOPHYSIOL@GICAI.
NATL%E
YASUNORI LNAGAKI, YOKO YOSHIDA, ?IARIKO KAN%AhI, YOUICHIROU HAMASAEI AND HIROAKI UEKI Department of Dermatology, Kawasaki Medical School,
Okayama
A novel autoantibody reactive with carbonic anhvdraseICA) has been found in sera from patients several with systemic autoimmune diseases, including SLE, SiGrren’s svndrome. Progressive Sysremic Sclerosis,-Dermatomvosltis, . ElCTA and so on. The autoantibody varied in the enrent of its crossreactivity among human CA I, human CA II, bovine CA II. rabbit CA, and dog CA. The titres continued to float and tended to parallel disease activity. Furthermore, the autoantibody may reduced the enzymic activitv.