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Anti-neutrophil cytoplash antibodies in systemic vasculitis
ANTI-NEUTRCPHIL CYTOPLAS;; ANTIBOCIES I N S Y S T W I C VASCULITf S J A S a v i q e , L Chang a n d .IT!P a r k i n , Department of Haerna t c . l o g y , R e p a t r i a t i o n G e n e r a l H o s p i t a l , H e i d e l b e r g W e s t , V i c , 3081.
A n t i - n e i i t r o p h i l c y t o p l a s m a n t i b o d i e s (ANCA) a r e commonly f o u n d i n Wegener's g r a n u l o m a t o s i s a n d m i c r o s c o p i c p o l y a r t e r i t i s . We h a v e t e s t e d 4 0 5 sera from p a t i e n t s w i t h a v a s c u l i t i s f o r t h e s e a n t i b o d i e s . One h u n d r e d and t w e n t y - s i x sera (25%) w e r e p o s i t i v e i n t h e a c i d e x t r a c t e x t r a c t ELISA ( c o n t a i n i n g n e u t r o p h i l p r o t e i n a s e 3 ) , 68 (14%) i n t h e a s s a y f o r a n t i -myeloperoxidase a n t i b o d i e s and 35 (16%) i n t h e a s s a y f o r a n t i - e l a s t a s e a n t i b o d i e s . A t o t a l of 156 s e r a (34%) w e r e p o s i t i v e for antibodies againzt neutrophil c y t o p l a s m c o n s t i t u e n t s . No a n t i b o d i e s w e r e p r e s e n t i n 26 c o n v a l e s c e n t s e r a and t h e mean t i m e f o r d i z a p p e a r a n c e was 8 weeks. Of t h e 228 s e r a also t e s t e d for anti-GRM a n t i b o d i e s , 1 3 (5.7%) were p o s i t i v e , F o r t y - n i n e of t h e 74 s e r a (66%) t e s t e d f o r ANA w e r e p o s i t i v e . Thus anti-neutx-ophil p r o t e i n a s e 3 , anti-myelop e r o x i d a s e and a n t i - e l a s t i s e a n t i b o d i e s a r e common i n p a t i e n t s i n whom a v a s c u l i t i s i s s u s p e c t e d on c l i n i c a l a n d / o r h i s t o l o g i c a l g r o u n d s a n d t h e p r e s e n c e of t h e s e a n t i b o d i e s c o r r e l a t e s w i t h d i s e a s e a c t i v i t y . A s i n g l e ELISA f o r A W A i s i n s u f f i c i e n t t o d e t e c t all a n t i b o d i e s d i r e c t e d against neutrophil Cytoplasm constituents.
F r e s e n t e r : D r J u d y S a v i g e , Haematology Pepartmerit, RGH.
OESTROGEN AND PROGESTERONE RECEPTORS AND NUCLEAR PROLIFERATION ANTIGENS IN PULMONARY LYMPHANGIOLEIOMYOMATOSIS.
St.Vincent's Hospital and the University of Melbourne Pulmonary lymphangleiomyomatosis is a rare disorder, seen exclusively in females during the reproductive years and is characterised by proliferation of smooth muscle in the lung. It progresses to pulmonary failure and death, unless abated by menopause. Hormonal manipulation has been variably successful in altering the progress of the disease. Hormone receptors have been shown in cytosolic preparations of pulmonary tissue and immunocytochernicaily in frozen sections of individual case reports of the disease. Using routinely fixed paraffin embedded archival tissue, we have been able to demonstrate marked variation in oestrogen and progesterone receptor status in a series of eight cases. These findings may partly explain the inconsistent resubs of hormonal manipulation in the treatment of the disease. Presenter: 0.Machet. Anatomical Pathology, St. Vincent's Hospital, FITZROY.
IMMUNOHISTOCHEMLCAL DETECTION O F RAS P21 AND C-MYC P G ONCOGENE PKODIJCTS IN NORMAL A N D MALIGNANT HUMAN bCSOPHAGEAL TISSUES
Z.C. S h a n , *L. Ma, S . L . L a k e , Department of P a t h o l o g y , U n i v e r s i t y o f Hong Kong, Queen Mary H o s p i t a l , Hong Kong.
The e x p r e s s i o n o f oncogene p r o d u c t s i n 56 s u r g i i \ a l cesophagectomy s p e c i m e n s o f human o e s o p h a g e a l c a r c i n o m a s and a d j a c e n t e p i t h e l i a was s t u d i e d by immunohistochemical method. The e x p r e s s i o n of raj. p21 i n i n f i l t r a t i n g squmous c e l l c a r c i n o m a was s i g n i f i c a n t l y e l e v e t e d compared w i t h t h a t i n t h e i r norma: e j i t h c l i a l c o u n t e r p a r t s ( P > 0.005). The s ta i n i n g i I: t e n s i ty nnd d i s t ri bu t i o n were c orre 1a t ed t c t h e deg.i.ces of tumouL d i f f e r e n t i a t i o n . For camparisor,, a d e n o c a r c i n o m a s o f t h e g a s t r i c c a r d i h were a l s o s r i i d i e d and t h e s t a i n i n g p a t t e r n showed no a s s o c i a t i o n w l t h tumour d i f f e r e n t i a t i o n . The e x p r e s s i o n ,:f o-nyc p62 i n b o t h normal and c a r c i n o m a o f t.he oesophagus was s i m i l a r t o t h a t of r a s p 2 1 , b u t i n lower ex!,ression l e v e l . Presenter:
Dr. Lily Ma, Denartment o f P a t h o l o g y , U n i L e r s i t y o f Hong Kong
A n t i - n e i i t r o p h i l c y t o p l a s m a n t i b o d i e s (ANCA) a r e commonly f o u n d i n Wegener's g r a n u l o m a t o s i s a n d m i c r o s c o p i c p o l y a r t e r i t i s . We h a v e t e s t e d 4 0 5 sera from p a t i e n t s w i t h a v a s c u l i t i s f o r t h e s e a n t i b o d i e s . One h u n d r e d and t w e n t y - s i x sera (25%) w e r e p o s i t i v e i n t h e a c i d e x t r a c t e x t r a c t ELISA ( c o n t a i n i n g n e u t r o p h i l p r o t e i n a s e 3 ) , 68 (14%) i n t h e a s s a y f o r a n t i -myeloperoxidase a n t i b o d i e s and 35 (16%) i n t h e a s s a y f o r a n t i - e l a s t a s e a n t i b o d i e s . A t o t a l of 156 s e r a (34%) w e r e p o s i t i v e for antibodies againzt neutrophil c y t o p l a s m c o n s t i t u e n t s . No a n t i b o d i e s w e r e p r e s e n t i n 26 c o n v a l e s c e n t s e r a and t h e mean t i m e f o r d i z a p p e a r a n c e was 8 weeks. Of t h e 228 s e r a also t e s t e d for anti-GRM a n t i b o d i e s , 1 3 (5.7%) were p o s i t i v e , F o r t y - n i n e of t h e 74 s e r a (66%) t e s t e d f o r ANA w e r e p o s i t i v e . Thus anti-neutx-ophil p r o t e i n a s e 3 , anti-myelop e r o x i d a s e and a n t i - e l a s t i s e a n t i b o d i e s a r e common i n p a t i e n t s i n whom a v a s c u l i t i s i s s u s p e c t e d on c l i n i c a l a n d / o r h i s t o l o g i c a l g r o u n d s a n d t h e p r e s e n c e of t h e s e a n t i b o d i e s c o r r e l a t e s w i t h d i s e a s e a c t i v i t y . A s i n g l e ELISA f o r A W A i s i n s u f f i c i e n t t o d e t e c t all a n t i b o d i e s d i r e c t e d against neutrophil Cytoplasm constituents.
F r e s e n t e r : D r J u d y S a v i g e , Haematology Pepartmerit, RGH.
OESTROGEN AND PROGESTERONE RECEPTORS AND NUCLEAR PROLIFERATION ANTIGENS IN PULMONARY LYMPHANGIOLEIOMYOMATOSIS.
St.Vincent's Hospital and the University of Melbourne Pulmonary lymphangleiomyomatosis is a rare disorder, seen exclusively in females during the reproductive years and is characterised by proliferation of smooth muscle in the lung. It progresses to pulmonary failure and death, unless abated by menopause. Hormonal manipulation has been variably successful in altering the progress of the disease. Hormone receptors have been shown in cytosolic preparations of pulmonary tissue and immunocytochernicaily in frozen sections of individual case reports of the disease. Using routinely fixed paraffin embedded archival tissue, we have been able to demonstrate marked variation in oestrogen and progesterone receptor status in a series of eight cases. These findings may partly explain the inconsistent resubs of hormonal manipulation in the treatment of the disease. Presenter: 0.Machet. Anatomical Pathology, St. Vincent's Hospital, FITZROY.
IMMUNOHISTOCHEMLCAL DETECTION O F RAS P21 AND C-MYC P G ONCOGENE PKODIJCTS IN NORMAL A N D MALIGNANT HUMAN bCSOPHAGEAL TISSUES
Z.C. S h a n , *L. Ma, S . L . L a k e , Department of P a t h o l o g y , U n i v e r s i t y o f Hong Kong, Queen Mary H o s p i t a l , Hong Kong.
The e x p r e s s i o n o f oncogene p r o d u c t s i n 56 s u r g i i \ a l cesophagectomy s p e c i m e n s o f human o e s o p h a g e a l c a r c i n o m a s and a d j a c e n t e p i t h e l i a was s t u d i e d by immunohistochemical method. The e x p r e s s i o n of raj. p21 i n i n f i l t r a t i n g squmous c e l l c a r c i n o m a was s i g n i f i c a n t l y e l e v e t e d compared w i t h t h a t i n t h e i r norma: e j i t h c l i a l c o u n t e r p a r t s ( P > 0.005). The s ta i n i n g i I: t e n s i ty nnd d i s t ri bu t i o n were c orre 1a t ed t c t h e deg.i.ces of tumouL d i f f e r e n t i a t i o n . For camparisor,, a d e n o c a r c i n o m a s o f t h e g a s t r i c c a r d i h were a l s o s r i i d i e d and t h e s t a i n i n g p a t t e r n showed no a s s o c i a t i o n w l t h tumour d i f f e r e n t i a t i o n . The e x p r e s s i o n ,:f o-nyc p62 i n b o t h normal and c a r c i n o m a o f t.he oesophagus was s i m i l a r t o t h a t of r a s p 2 1 , b u t i n lower ex!,ression l e v e l . Presenter:
Dr. Lily Ma, Denartment o f P a t h o l o g y , U n i L e r s i t y o f Hong Kong