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Antibacterial activity of Nicotiana tabacum leaves
Fitoterapia 71 Ž2000. 199]200
Short report
Antibacterial activity of Nicotiana tabacum leaves David A. Akinpelua,U , Efere M. Obuotor b a b
Department of Microbiology, Obafemi Awolowo Uni¨ ersity, Ile-Ife, Osun State, Nigeria Department of Biochemistry, Obafemi Awolowo Uni¨ ersity, Ile-Ife, Osun State, Nigeria Received 28 June 1999; accepted in revised form 30 September 1999
Abstract Nicotiana tabacum leaf 60% methanolic extract exhibited antibacterial activity against six out of nine bacterial isolates at a concentration of 25 mgrml. Q 2000 Elsevier Science B.V. All rights reserved. Keywords: Nicotiana tabacum; Antibacterial activity
Plant. Nicotiana tabacum L. ŽSolanacee. leaves were collected at the Research Farm of Obafemi Awolowo University, Ile-Ife, Nigeria in September 1998. The plant was identified by Dr H.C. Illoh of the Department of Botany, Obafemi Awolowo University. Uses in traditional medicine. The leaves of the plant are used for the treatment of toothache, respiratory complications and insect bites w1x. Tested material. 60% MeOH extract Žyield: 13.7 g from 87.5 g of air-dried leaves.. Phytochemical analysis w2x gave positive tests for alkaloids and flavonoids. Studied activity. Antibacterial activity by agar-well diffusion method w3x and minimum inhibitory concentration w4x. U
Corresponding author. E-mail address: [email protected] ŽD.A. Akinpelu.
0367-326Xr00r$ - see front matter Q 2000 Elsevier Science B.V. All rights reserved. PII: S 0 3 6 7 - 3 2 6 X Ž 9 9 . 0 0 1 4 8 - 3
200
D.A. Akinpelu, E.M. Obuotor r Fitoterapia 71 (2000) 199]200
Table 1 Antibacterial activity of Nicotiana tabacum leaf extract a Microorganisms
Bacillus subtilis ŽNCIB 3610. Corynebacterium pyogenes ŽLIO. Escherichia coli ŽNCIB 86. Klebsiella pneumoniae ŽNCIB 418. Proteus ¨ ulgaris ŽNCIB 67. Pseudomonas aeruginosa ŽNCIB 950. Serratia marcescens ŽNCIB 1377. Shigella dysenteriae ŽLIO. Staphylococcus aureus ŽNCIB 8588.
Zone of inhibition Žmm. N. tabacum Ž25 mgrml.
Streptomycin Ž1 mgrml.
MIC Žmgrml.
13 15 0 0 0 11 11 15 14
23 20 0 0 28 21 22 23 21
6.25 6.25 ] ] ] 12.5 12.5 1.56 3.13
a
Values are the mean of three replicates; MIC, minimum inhibitory concentration; LIO, locally isolated organism.
Used microorganisms. Listed in Table 1. Results. Reported in Table 1. Conclusions. N. tabacum leaf extract exhibited antibacterial activity against six out of nine bacteria used at a concentration of 25 mgrml. E. coli, K. pneumoniae and P. ¨ ulgaris were not inhibited . However, E. coli and K. pneumoniae were also not sensitive to streptomycin.
References w1x Dalziel JM. The useful plants of west tropical Africa. London: Crown Agents for the Colonies, 1937:430. w2x Harborne JB. Phytochemical methods. London: Chapman and Hall, 1973:278. w3x Kavanagh F. Analytical microbiology. New York: Academic Press, 1963:290. w4x Russell AD, Furr JR. J Appl Bacteriol 1977;43:253.
Short report
Antibacterial activity of Nicotiana tabacum leaves David A. Akinpelua,U , Efere M. Obuotor b a b
Department of Microbiology, Obafemi Awolowo Uni¨ ersity, Ile-Ife, Osun State, Nigeria Department of Biochemistry, Obafemi Awolowo Uni¨ ersity, Ile-Ife, Osun State, Nigeria Received 28 June 1999; accepted in revised form 30 September 1999
Abstract Nicotiana tabacum leaf 60% methanolic extract exhibited antibacterial activity against six out of nine bacterial isolates at a concentration of 25 mgrml. Q 2000 Elsevier Science B.V. All rights reserved. Keywords: Nicotiana tabacum; Antibacterial activity
Plant. Nicotiana tabacum L. ŽSolanacee. leaves were collected at the Research Farm of Obafemi Awolowo University, Ile-Ife, Nigeria in September 1998. The plant was identified by Dr H.C. Illoh of the Department of Botany, Obafemi Awolowo University. Uses in traditional medicine. The leaves of the plant are used for the treatment of toothache, respiratory complications and insect bites w1x. Tested material. 60% MeOH extract Žyield: 13.7 g from 87.5 g of air-dried leaves.. Phytochemical analysis w2x gave positive tests for alkaloids and flavonoids. Studied activity. Antibacterial activity by agar-well diffusion method w3x and minimum inhibitory concentration w4x. U
Corresponding author. E-mail address: [email protected] ŽD.A. Akinpelu.
0367-326Xr00r$ - see front matter Q 2000 Elsevier Science B.V. All rights reserved. PII: S 0 3 6 7 - 3 2 6 X Ž 9 9 . 0 0 1 4 8 - 3
200
D.A. Akinpelu, E.M. Obuotor r Fitoterapia 71 (2000) 199]200
Table 1 Antibacterial activity of Nicotiana tabacum leaf extract a Microorganisms
Bacillus subtilis ŽNCIB 3610. Corynebacterium pyogenes ŽLIO. Escherichia coli ŽNCIB 86. Klebsiella pneumoniae ŽNCIB 418. Proteus ¨ ulgaris ŽNCIB 67. Pseudomonas aeruginosa ŽNCIB 950. Serratia marcescens ŽNCIB 1377. Shigella dysenteriae ŽLIO. Staphylococcus aureus ŽNCIB 8588.
Zone of inhibition Žmm. N. tabacum Ž25 mgrml.
Streptomycin Ž1 mgrml.
MIC Žmgrml.
13 15 0 0 0 11 11 15 14
23 20 0 0 28 21 22 23 21
6.25 6.25 ] ] ] 12.5 12.5 1.56 3.13
a
Values are the mean of three replicates; MIC, minimum inhibitory concentration; LIO, locally isolated organism.
Used microorganisms. Listed in Table 1. Results. Reported in Table 1. Conclusions. N. tabacum leaf extract exhibited antibacterial activity against six out of nine bacteria used at a concentration of 25 mgrml. E. coli, K. pneumoniae and P. ¨ ulgaris were not inhibited . However, E. coli and K. pneumoniae were also not sensitive to streptomycin.
References w1x Dalziel JM. The useful plants of west tropical Africa. London: Crown Agents for the Colonies, 1937:430. w2x Harborne JB. Phytochemical methods. London: Chapman and Hall, 1973:278. w3x Kavanagh F. Analytical microbiology. New York: Academic Press, 1963:290. w4x Russell AD, Furr JR. J Appl Bacteriol 1977;43:253.