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Antibacterial and antifungal activity of charcoal materials and microwave radiation
SYMPOSIUM 8: INDUSTRIAL BIOTECHNOLOGY OF NATURAL AND SYNTHETIC POLYMERS
O8-4 Influence of nutritional and physicochemical variables in PHB production from raw glycerol by a wild Bacillus megaterium strain Carolina Guzmán Luna 1,∗ , Paalo Andrea Moreno Ya˜nez 1 , Camilo Jose Ya˜nez Diaz 1 , Nilo Sérgio Medeiros Cardozo 2 , Humberto Escalante 1 , Marianny Y. Combariza 1 1
2
Universidad Industrial de Santander, Brazil Federal University of Rio Grande do Sul, Brazil
Reducing production costs and increasing strain productivity in biosynthetic processes are decisive factors linked to biodegradable polymers worldwide production and usage. Agro-industrial byproducts as alternative carbon sources and optimization of culture media composition are some of the strategies used to address these issues. Amongst many available biopolymers polyhydroxybutyrate (PHB), intracellularly produced and accumulated by abundant bacteria species, could become a substitute for synthetic plastics due to its thermoplastic and mechanical properties. In this contribution we report on the influence of five nutritional and two physicochemical variables in PHB production by Bacillus megaterium B2, a recently isolated and characterized bacterium with PHB accumulating ability using raw glycerol, from the biodiesel industry, as carbon source [1]. According to Plackett Burman and central composite designs temperature, glycerol and Na2 HPO4 concentrations are the most significant variables on PHB production by B2, with optimal values of 34 ◦ C, 7.6 g/L and 3 g/L, respectively. After 14 hours of fermentation, in shake flasks with optimized medium, B2 produced 0.37 g/L of PHB with an 18% w/w accumulation. This corresponds to an 85% increase in PHB production when compared to initial culture conditions. These results suggest the potential of B. megaterium B2 as PHB producer using raw glycerol an inexpensive, abundant and readily available carbon source. Reference [1].Tarazona N, Moreno P, Ya˜ nez C, Combariza MY, Guzmán C. Microbial production of polyhydroxybutyrate by native Bacillus strains using a biodiesel by-product as carbon source. Lisbon, Portugal: Presented at the European Symposium on Biopolymers; October 7–9, 2013. PS 2.1.
http://dx.doi.org/10.1016/j.nbt.2014.05.1685
O8-5 Value-added carotenoid production in the pennate diatom Phaeodactylum tricornutum with light emitting diode based photobioreactors Weiqi Fu 1,∗ , Sigurður Brynjólfsson 1 , Bernhard Palsson 2 1
2
University of Iceland, Iceland University of California, San Diego, United States
Diatoms are one of the most promising feedstocks for producing feed supplements, bioactive pharmaceuticals and biofuels in a bio-based economy. The marine pennate diatom PhaeodactyS32
www.elsevier.com/locate/nbt
New Biotechnology · Volume 31S · July 2014
lum tricornutum is able to accumulate large amounts of specialty carotenoids. Among the valuable carotenoids present in diatoms, fucoxanthin has health promoting effects in humans, including anti-cancer, anti-obesity, and anti-diabetic effects as well as antimalarial activity. Fucoxanthin has also been observed to be more potent than -carotene and astaxanthin in terms of anti-obesity and anti-proliferative effects on adult T-cell leukemia cells. The demand for fucoxanthin in the global market has been increasing dramatically. In this study, we characterized the biomass productivity and biomass yield of P. tricornutum with light emitting diode (LED)-based photobioreactors with different light intensities and wavelengths. Under red LED illumination, increasing the photon flux from 85 to 255 E/m2 /s caused photoinhibition of Phaeodactylum cells when there was either 3.0 mM or 0.3 mM of metasilicate in the medium. In contrast, combined red and blue (50:50) LED illumination produced a higher biomass yield and growth rate. Further, the presence of blue light enhanced the accumulation of fucoxanthin in comparison to illumination with red light only. These results demonstrated the feasibility of fucoxanthin production in P. tricornutum, and a method combining LED technology and synthetic biology approaches was proposed to facilitate its development. http://dx.doi.org/10.1016/j.nbt.2014.05.1686
O8-6 Antibacterial and antifungal activity of charcoal materials and microwave radiation Hee Jin Yang 1,∗ , Yun Jeong Cha 2 , Hern Kim 2 , Shin Sik Choi 2,3 1
Myoungji University, Republic of Korea Department of Energy and Biotechnology, Myongji University, Republic of Korea 3 Department of Food and Nutrition, Myongji University, Republic of Korea 2
Suppression of microbial contamination or growth using antibacterial and antifungal materials has been required in various commercial products including foods and cosmetics. In this study, we have investigated the inhibitory effect of charcoal polymers and microwave radiation on bacterial and fungal cell growth or survival rate. When bacterial and fungal cells were cultured with charcoal polymers, the growth of some microbes was significantly inhibited by nano and micro sized charcoal particles protruded from the surface of polymers. Four species of fungi were also dramatically killed by microwave radiation for less than 5 min. These results suggest that the use of charcoal plastics and microwave has a great potential for reducing microbial contamination in multiple areas. Acknowledgements: This work was supported by NGV of Hyundai Motors Company and BK21 Plus of Ministry of Education (22A20130012051). http://dx.doi.org/10.1016/j.nbt.2014.05.1687
O8-4 Influence of nutritional and physicochemical variables in PHB production from raw glycerol by a wild Bacillus megaterium strain Carolina Guzmán Luna 1,∗ , Paalo Andrea Moreno Ya˜nez 1 , Camilo Jose Ya˜nez Diaz 1 , Nilo Sérgio Medeiros Cardozo 2 , Humberto Escalante 1 , Marianny Y. Combariza 1 1
2
Universidad Industrial de Santander, Brazil Federal University of Rio Grande do Sul, Brazil
Reducing production costs and increasing strain productivity in biosynthetic processes are decisive factors linked to biodegradable polymers worldwide production and usage. Agro-industrial byproducts as alternative carbon sources and optimization of culture media composition are some of the strategies used to address these issues. Amongst many available biopolymers polyhydroxybutyrate (PHB), intracellularly produced and accumulated by abundant bacteria species, could become a substitute for synthetic plastics due to its thermoplastic and mechanical properties. In this contribution we report on the influence of five nutritional and two physicochemical variables in PHB production by Bacillus megaterium B2, a recently isolated and characterized bacterium with PHB accumulating ability using raw glycerol, from the biodiesel industry, as carbon source [1]. According to Plackett Burman and central composite designs temperature, glycerol and Na2 HPO4 concentrations are the most significant variables on PHB production by B2, with optimal values of 34 ◦ C, 7.6 g/L and 3 g/L, respectively. After 14 hours of fermentation, in shake flasks with optimized medium, B2 produced 0.37 g/L of PHB with an 18% w/w accumulation. This corresponds to an 85% increase in PHB production when compared to initial culture conditions. These results suggest the potential of B. megaterium B2 as PHB producer using raw glycerol an inexpensive, abundant and readily available carbon source. Reference [1].Tarazona N, Moreno P, Ya˜ nez C, Combariza MY, Guzmán C. Microbial production of polyhydroxybutyrate by native Bacillus strains using a biodiesel by-product as carbon source. Lisbon, Portugal: Presented at the European Symposium on Biopolymers; October 7–9, 2013. PS 2.1.
http://dx.doi.org/10.1016/j.nbt.2014.05.1685
O8-5 Value-added carotenoid production in the pennate diatom Phaeodactylum tricornutum with light emitting diode based photobioreactors Weiqi Fu 1,∗ , Sigurður Brynjólfsson 1 , Bernhard Palsson 2 1
2
University of Iceland, Iceland University of California, San Diego, United States
Diatoms are one of the most promising feedstocks for producing feed supplements, bioactive pharmaceuticals and biofuels in a bio-based economy. The marine pennate diatom PhaeodactyS32
www.elsevier.com/locate/nbt
New Biotechnology · Volume 31S · July 2014
lum tricornutum is able to accumulate large amounts of specialty carotenoids. Among the valuable carotenoids present in diatoms, fucoxanthin has health promoting effects in humans, including anti-cancer, anti-obesity, and anti-diabetic effects as well as antimalarial activity. Fucoxanthin has also been observed to be more potent than -carotene and astaxanthin in terms of anti-obesity and anti-proliferative effects on adult T-cell leukemia cells. The demand for fucoxanthin in the global market has been increasing dramatically. In this study, we characterized the biomass productivity and biomass yield of P. tricornutum with light emitting diode (LED)-based photobioreactors with different light intensities and wavelengths. Under red LED illumination, increasing the photon flux from 85 to 255 E/m2 /s caused photoinhibition of Phaeodactylum cells when there was either 3.0 mM or 0.3 mM of metasilicate in the medium. In contrast, combined red and blue (50:50) LED illumination produced a higher biomass yield and growth rate. Further, the presence of blue light enhanced the accumulation of fucoxanthin in comparison to illumination with red light only. These results demonstrated the feasibility of fucoxanthin production in P. tricornutum, and a method combining LED technology and synthetic biology approaches was proposed to facilitate its development. http://dx.doi.org/10.1016/j.nbt.2014.05.1686
O8-6 Antibacterial and antifungal activity of charcoal materials and microwave radiation Hee Jin Yang 1,∗ , Yun Jeong Cha 2 , Hern Kim 2 , Shin Sik Choi 2,3 1
Myoungji University, Republic of Korea Department of Energy and Biotechnology, Myongji University, Republic of Korea 3 Department of Food and Nutrition, Myongji University, Republic of Korea 2
Suppression of microbial contamination or growth using antibacterial and antifungal materials has been required in various commercial products including foods and cosmetics. In this study, we have investigated the inhibitory effect of charcoal polymers and microwave radiation on bacterial and fungal cell growth or survival rate. When bacterial and fungal cells were cultured with charcoal polymers, the growth of some microbes was significantly inhibited by nano and micro sized charcoal particles protruded from the surface of polymers. Four species of fungi were also dramatically killed by microwave radiation for less than 5 min. These results suggest that the use of charcoal plastics and microwave has a great potential for reducing microbial contamination in multiple areas. Acknowledgements: This work was supported by NGV of Hyundai Motors Company and BK21 Plus of Ministry of Education (22A20130012051). http://dx.doi.org/10.1016/j.nbt.2014.05.1687