- Email: [email protected]
Antibacterial effect of methanolic extract of Aronia melanocarpa against Salmonella typhimurium strains isolated from wild birds
S106
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
evaluated by using NTSYSpc 2.1. computer software. Evaluations were conducted according to the comparison of naturally occurring polymorphism levels and salinity-induced polymorphism levels. The findings of the recent study will contribute to abiotic stress response differences related to Tos-17 transposon mobility of rice plants against stress conditions. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1161 Two-dimensional protein profiles of drought tolerant tomato (Solanum lycopersicum) mutants improved by gamma radiation Alp Ayan, Ozge C¸elik, Sinan Meric¸, Burak Topc¸uoglu ∗ , C¸imen Atak Department of Molecular Biology and Genetics, Istanbul Kultur University, Istanbul, Turkey E-mail address: [email protected] (B. Topc¸uoglu). This study is aimed to identify two-dimensional protein profiles of mutant plants derived from industrial 5MX12956 tomato (Solanum lycopersicum) variety by using Cs-137 gamma source. Drought tolerant tomato mutants were selected through in vivo withholding-watering for 7 days in M2 generation. In vitro drought tolerance tests were performed under 5% PEG treatments for 14 days according to our preliminary studies. 2 of 72 drought tolerant mutants (5-34-1 and 5-34-11) presented 150% and 192% better callus growth under in vitro drought treatments, respectively. Protein extractions of the mutant plants were performed by TCA/Acetone protein precipitation protocol. Proteins were analyzed in Bio-rad Protean Xi Cell 2-D system. Protein spots were visualized by coomassie staining. Detected spots were purified by in-gel digestion method and analyzed via MS/MS mass spectrometry to identify proteomic differences caused by random mutations of drought tolerant mutants. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1162 Magnetic field induced production of anti-cancer agent vincristine from Madagascar Periwinkle (Catharanthus roseus) plant
production were evaluated by Fourier Transform Infrared Spectroscopy (FT-IR) analysis. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1163 Antibacterial effect of methanolic extract of Aronia melanocarpa against Salmonella typhimurium strains isolated from wild birds Diana Olah 1,∗ , Mihaela Niculae 1 , Carmen Dana Sandru 1 , Zorita Diaconeasa 2 , Marina Spinu 1 , Constantin Cerbu 1 , Emoke Pall 1 1
Department of Infectious Diseases, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania 2 Faculty of Food Science and Technology, University of Agricultural Science and Veterinary Medicine, Cluj-Napoca, Romania E-mail address: [email protected] (D. Olah). The present study investigates the effects of methanolic extract of Aronia melanocarpa on Salmonella typhimurium strains isolated from wild birds. Crude methanolic extracts of A. melanocarpa were obtained from fresh fruits, anthocyanin determination was performed using HPLC analysis and the total phenolic content was evaluated by Folin–Ciocalteau reagent based assay. Antimicrobial activity was determined by the agar well diffusion method. Strains were isolated from migratory birds and sedentary birds captured in Danube Delta Biosphere Reserve, such as the Eurasian sparrow hawk (Accipiter nisus), common chaffinch (Fringilla coelebs), great tit (Parus major), reed bunting (Emberiza schoeniclus), Eurasian sparrowhawk (Accipiter nisus), common snipe (Gallinago gallinago), and Eurasian tree sparrow (Passer montanus). All experiments were performed in triplicates. All values are expressed as mean ± standard deviation (SD). Our results indicated that methanolic extract of Aronia melanocarpa has antibacterial properties against Salmonella typhimurium strains isolated from wild birds, and could be considered as alternatives in the treatment of bacterial infections. Acknowledgement: This study was supported by the PNII/PCCA 61/2012 grant of the National Council of Scientific University Research of Romania.
Ozge C¸elik, Sinan Meric¸, Alp Ayan, Meric¸ Tas ∗ , Ceren Avcılar, C¸imen Atak
http://dx.doi.org/10.1016/j.jbiotec.2017.06.1164
Department of Molecular Biology and Genetics, Istanbul Kultur University, Istanbul, Turkey
Reduction of in vitro plant growth as an effect of pathogenic bacteria interaction
E-mail address: [email protected] (M. Tas).
Włodzimierz Przewodowski, Agnieszka Przewodowska ∗ , Dorota Michałowska, Katarzyna ´ Salamonska, Dorota Szarek, Wioleta Stochła, Danuta Sekrecka
Catharanthus roseus known as Madagaskar periwinkle, is an endemic flowering plant which has important medical products as vincristine and vinblastine. Vincristine and vinblastine, which are widely used as anti-cancer drugs, inhibit cell proliferation during mitotic processes. Magnetic field is known to enhance plant growth and metabolite production. Aim of this study is inducing vincristine production by using magnetic field. In this context, we applied 4.6 mT magnetic field in 2.2, 6.6, 19.8 and 33 s to induce metabolite production in Catharanthus roseus seeds. Seeds were planted to Murashige & Skoog (MS) medium supplemented with 5 mg/l NAA and 3 mg/l BAP plant growth regulators after magnetic field treatment. Vincristine was extracted from 4 weeks-old plant leaves by using methanol as solvent and ethyl acetate to form organic phase separation. Effects of magnetic field treatments on vincristine
Plant Breeding and Acclimatization Institute – National Research Institute, Bonin Research Center, Poland E-mail address: [email protected] (A. Przewodowska). Micropropagation is one of the in vitro reproduction technique allowing to obtain a large amount of plant material in a relatively short time. Potato in vitro plantlets are particularly susceptible to bacterial infection because of their specific morphology, size and growth in artificial conditions. Therefore, it is important to provide sterile conditions during the process and to prevent infection during the mass production process. The most undesirable microorganisms in this case are quarantine bacterium, like [Clavibacter michiganensis] ssp. [sepedonicus] (Cms) which excludes the
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
evaluated by using NTSYSpc 2.1. computer software. Evaluations were conducted according to the comparison of naturally occurring polymorphism levels and salinity-induced polymorphism levels. The findings of the recent study will contribute to abiotic stress response differences related to Tos-17 transposon mobility of rice plants against stress conditions. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1161 Two-dimensional protein profiles of drought tolerant tomato (Solanum lycopersicum) mutants improved by gamma radiation Alp Ayan, Ozge C¸elik, Sinan Meric¸, Burak Topc¸uoglu ∗ , C¸imen Atak Department of Molecular Biology and Genetics, Istanbul Kultur University, Istanbul, Turkey E-mail address: [email protected] (B. Topc¸uoglu). This study is aimed to identify two-dimensional protein profiles of mutant plants derived from industrial 5MX12956 tomato (Solanum lycopersicum) variety by using Cs-137 gamma source. Drought tolerant tomato mutants were selected through in vivo withholding-watering for 7 days in M2 generation. In vitro drought tolerance tests were performed under 5% PEG treatments for 14 days according to our preliminary studies. 2 of 72 drought tolerant mutants (5-34-1 and 5-34-11) presented 150% and 192% better callus growth under in vitro drought treatments, respectively. Protein extractions of the mutant plants were performed by TCA/Acetone protein precipitation protocol. Proteins were analyzed in Bio-rad Protean Xi Cell 2-D system. Protein spots were visualized by coomassie staining. Detected spots were purified by in-gel digestion method and analyzed via MS/MS mass spectrometry to identify proteomic differences caused by random mutations of drought tolerant mutants. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1162 Magnetic field induced production of anti-cancer agent vincristine from Madagascar Periwinkle (Catharanthus roseus) plant
production were evaluated by Fourier Transform Infrared Spectroscopy (FT-IR) analysis. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1163 Antibacterial effect of methanolic extract of Aronia melanocarpa against Salmonella typhimurium strains isolated from wild birds Diana Olah 1,∗ , Mihaela Niculae 1 , Carmen Dana Sandru 1 , Zorita Diaconeasa 2 , Marina Spinu 1 , Constantin Cerbu 1 , Emoke Pall 1 1
Department of Infectious Diseases, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania 2 Faculty of Food Science and Technology, University of Agricultural Science and Veterinary Medicine, Cluj-Napoca, Romania E-mail address: [email protected] (D. Olah). The present study investigates the effects of methanolic extract of Aronia melanocarpa on Salmonella typhimurium strains isolated from wild birds. Crude methanolic extracts of A. melanocarpa were obtained from fresh fruits, anthocyanin determination was performed using HPLC analysis and the total phenolic content was evaluated by Folin–Ciocalteau reagent based assay. Antimicrobial activity was determined by the agar well diffusion method. Strains were isolated from migratory birds and sedentary birds captured in Danube Delta Biosphere Reserve, such as the Eurasian sparrow hawk (Accipiter nisus), common chaffinch (Fringilla coelebs), great tit (Parus major), reed bunting (Emberiza schoeniclus), Eurasian sparrowhawk (Accipiter nisus), common snipe (Gallinago gallinago), and Eurasian tree sparrow (Passer montanus). All experiments were performed in triplicates. All values are expressed as mean ± standard deviation (SD). Our results indicated that methanolic extract of Aronia melanocarpa has antibacterial properties against Salmonella typhimurium strains isolated from wild birds, and could be considered as alternatives in the treatment of bacterial infections. Acknowledgement: This study was supported by the PNII/PCCA 61/2012 grant of the National Council of Scientific University Research of Romania.
Ozge C¸elik, Sinan Meric¸, Alp Ayan, Meric¸ Tas ∗ , Ceren Avcılar, C¸imen Atak
http://dx.doi.org/10.1016/j.jbiotec.2017.06.1164
Department of Molecular Biology and Genetics, Istanbul Kultur University, Istanbul, Turkey
Reduction of in vitro plant growth as an effect of pathogenic bacteria interaction
E-mail address: [email protected] (M. Tas).
Włodzimierz Przewodowski, Agnieszka Przewodowska ∗ , Dorota Michałowska, Katarzyna ´ Salamonska, Dorota Szarek, Wioleta Stochła, Danuta Sekrecka
Catharanthus roseus known as Madagaskar periwinkle, is an endemic flowering plant which has important medical products as vincristine and vinblastine. Vincristine and vinblastine, which are widely used as anti-cancer drugs, inhibit cell proliferation during mitotic processes. Magnetic field is known to enhance plant growth and metabolite production. Aim of this study is inducing vincristine production by using magnetic field. In this context, we applied 4.6 mT magnetic field in 2.2, 6.6, 19.8 and 33 s to induce metabolite production in Catharanthus roseus seeds. Seeds were planted to Murashige & Skoog (MS) medium supplemented with 5 mg/l NAA and 3 mg/l BAP plant growth regulators after magnetic field treatment. Vincristine was extracted from 4 weeks-old plant leaves by using methanol as solvent and ethyl acetate to form organic phase separation. Effects of magnetic field treatments on vincristine
Plant Breeding and Acclimatization Institute – National Research Institute, Bonin Research Center, Poland E-mail address: [email protected] (A. Przewodowska). Micropropagation is one of the in vitro reproduction technique allowing to obtain a large amount of plant material in a relatively short time. Potato in vitro plantlets are particularly susceptible to bacterial infection because of their specific morphology, size and growth in artificial conditions. Therefore, it is important to provide sterile conditions during the process and to prevent infection during the mass production process. The most undesirable microorganisms in this case are quarantine bacterium, like [Clavibacter michiganensis] ssp. [sepedonicus] (Cms) which excludes the