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Antidepressant like effect of hydro-alcoholic extract of Nigella sativa in tail suspension test in mice
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P.2.a. Mood disorders and treatment − Affective disorders (basic)
[4] Abdallah, C.G., Jackowski, A., Sato, J.R., Mao, X., Kang, G., Cheema, R., Coplan, J.D., Mathew, S.J., Shungu, D.C., 2015. Prefrontal cortical GABA abnormalities are associated with reduced hippocampal volume in major depressive disorder. Eur Neuropsychopharmacol 25, 1082–1090. [5] Cz´eh, B., Varga, Z.K., Henningsen, K., Kov´acs, G.L., Miseta, A., Wiborg, O., 2015. Chronic stress reduces the number of GABAergic interneurons in the adult rat hippocampus, dorsal-ventral and regionspecific differences. Hippocampus 25, 393–405. Disclosure statement: This work has been supported by the Hungarian Brain Research Program KTIA_NAP_13−2–2014–0019 and by the Lundbeck Foundation R83-A7631.
decrease in immobility time but it was not statistically significant. There was no change in locomotor activity in N. sativa doses which are applied. Conclusion: The results of this preliminary study showed that hydro-alcoholic extract of N. sativa has an antidepressant like effect. This results has confirmed the previous studies. Since whole extract was used, it is necessary to investigate all biologically active groups in the extract and compare with synthetic equivalants. Further studies are also needed to understand the mechanism of the action. References
P.2.a.012 Antidepressant like effect of hydro-alcoholic extract of Nigella sativa in tail suspension test in mice A.N. Hazar-Yavuz1 ° , R. Keles1 , G. Unal1 , T. Taskin2 , F. Aricioglu1 1 Marmara University, Faculty of PharmacyDepartment of Pharmacology and Psychopharmacology Research Unit, Istanbul, Turkey; 2 Marmara University, Faculty of Pharmacy- Department of Pharmacognosy, Istanbul, Turkey Objective: Nigella sativa, often called black cumin, has a rich historical background since many researches showed its wide spectrum of pharmacological potential. The seeds, oil and various extracts of N. sativa have been widely used for centuries in the treatment of various ailments. N. sativa has a number of pharmacological activities such as antibacterial, antifungal, antioxidant, antiarthritic, antidiabetic, anticancer, antiinflammatory, analgesic, immunomodulatory, cardiovascular, gastroprotective, hepatoprotective, nephroprotective, antiasthmatic and anticonvulsant [1]. Moreover it has been shown to have central effects, especially anxiolytic, antidepressant actions and improving learning and memory [1,2]. Repeated administration of N. sativa oil in rats decreases serotonergic turnover and produces anxiolytic effects in open field test and elevated plus maze test [3] and suggested that a long term administration may enhance learning and memory by increasing serotonine levels in rats [1]. The results of previous experimental studies have supported the pharmacological effects of the seeds of N. sativa or major ingradient thymoquinone. Moreover N. sativa has showen to prevent lipopolysaccharideinduced depression like behavior in rats [4]. The aim of the present study was to investigate potential acute antidepressant effect of hydro-alcoholic N. sativa extract in the tail suspension test. Methods: 27 Balb/C mice (25−35 g) were divided into Control (n = 10), Imipramine (10 mg/kg, i.p., n = 5), N. sativa (250 mg/kg, i.p., n = 6), N. sativa (500 mg/kg, i.p., n = 6) groups. Imipramine and hydro-alcoholic extracts (70 ethanol: 30 water %) of N. sativa seeds were administered 30 min prior to tail suspension test. Mice were tested on open field in order to make sure that chosen doses have no effect on locomotory activity. For tail suspension test, mice were suspended in a frame 40 cm above the floor using a paper adhesive tape that placed approximately 1 cm from the tip of the tail. Time of immobility was defined as a complete lack of movement and scored during 5 minutes. The differences between groups were compared by one-way ANOVA and post hoc Dunnett’s test for statistical analyses and p values <0.05 considered statistically significant. Results: Immobility time was significantly reduced in imipramine group compared to control group (p < 0.05). N. sativa (500 mg/kg) significantly decreased the immobility time compared to control. In N. sativa (250 mg/kg) group, there was a tendency of
[1] Ahmad, A., Husain, A., Mujeeb, M., Khan, S.A., Najmi, A.K., Siddique, N.A., Damanhouri, Z.A., Anwar, F., 2013. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed 3(5), 337−52. [2] Al-Naggar, T.B., G´omez-Serranillos, M.P., Carretero, M.E., Villar, A.M., 2003. Neuropharmacological activity of Nigella sativa L. extracts. J Ethnopharmacol 88(1) 63−8. [3] Perveen, T., Haider, S., Kanwal, S., Haleem, D.J., 2009. Repeated administration of Nigella sativa decreases 5-HT turnover and produces anxiolytic effects in rats. Pak J Pharm Sci 22(2), 139–144. [4] Hosseini, M., Zakeri, S., Khoshdast, S., Yousefian, F.T., Rastegar, M., Vafaee, F., Kahdouee, S., Ghorbani, F., Rakhshandeh, H., Kazemi, S.A., 2012. The effects of Nigella sativa hydro-alcoholic extract and thymoquinone on lipopolysaccharide − induced depression like behavior in rats. J Pharm Bioallied Sci 4(3) 219−25. Disclosure statement: This abstract is financially supported by Scientific Research Project Unit of Marmara University SAG-E-120613–0233
P.2.a.014 Ketamine modulates catecholamine transmission in the bed nucleus of stria terminalis: a possible role of this region in the antidepressant effects of ketamine D. Jadzic1 ° , E.C. Carboni2 , R.C. Cadeddu1 , J.S. Samardzic3 1 University of Cagliari, Department of Biomedical Sciences, Cagliari, Italy; 2 University of Cagliari, Department of Biomedical Sciences- Neuroscience Institute- Section of Cagliari- National Research Council of Italy - CNR- Cagliari- Italy, Cagliari, Italy; 3 University of Belgrade, Institute of Pharmacology- Clinical Pharmacology and Toxicology- Medical Faculty- University of Belgrade- Belgrade- Serbia., Belgrade, Serbia Introduction: Considering that is widely accepted that depression can result from the combination of genetic and epigenetic factors, and that among the latter stress has a fundamental role [1], we identify a brain area involved in stress control, such as the bed nucleus of stria terminalis (BNST) as the target of this study. We recently showed that various antidepressants share the property of increasing dose dependently catecholamine transmission in the rats BNST [2] thus we intended to exanimate the effect of an unusual antidepressant such as ketamine. The non selective N-methyl-D-aspartate (NMDA) receptor antagonist has been suggested as a promising medication in a treatment of therapyresistant patients, evoking long-lasting effect already after infusion of a single dose [3]. Aim: Through the microdialysis technique in freely moving rats, we monitored the acute effects of ketamine on catecholamine release in the BNST, and compared those with the effects of typical and atypical antidepressants, in order to better understand the role of the BNST in antidepressant effect of ketamine. Methods: Norepinephrine (NE) and dopamine (DA) were assessed in dialysate samples by HPLC and coulometric detection
P.2.a. Mood disorders and treatment − Affective disorders (basic)
[4] Abdallah, C.G., Jackowski, A., Sato, J.R., Mao, X., Kang, G., Cheema, R., Coplan, J.D., Mathew, S.J., Shungu, D.C., 2015. Prefrontal cortical GABA abnormalities are associated with reduced hippocampal volume in major depressive disorder. Eur Neuropsychopharmacol 25, 1082–1090. [5] Cz´eh, B., Varga, Z.K., Henningsen, K., Kov´acs, G.L., Miseta, A., Wiborg, O., 2015. Chronic stress reduces the number of GABAergic interneurons in the adult rat hippocampus, dorsal-ventral and regionspecific differences. Hippocampus 25, 393–405. Disclosure statement: This work has been supported by the Hungarian Brain Research Program KTIA_NAP_13−2–2014–0019 and by the Lundbeck Foundation R83-A7631.
decrease in immobility time but it was not statistically significant. There was no change in locomotor activity in N. sativa doses which are applied. Conclusion: The results of this preliminary study showed that hydro-alcoholic extract of N. sativa has an antidepressant like effect. This results has confirmed the previous studies. Since whole extract was used, it is necessary to investigate all biologically active groups in the extract and compare with synthetic equivalants. Further studies are also needed to understand the mechanism of the action. References
P.2.a.012 Antidepressant like effect of hydro-alcoholic extract of Nigella sativa in tail suspension test in mice A.N. Hazar-Yavuz1 ° , R. Keles1 , G. Unal1 , T. Taskin2 , F. Aricioglu1 1 Marmara University, Faculty of PharmacyDepartment of Pharmacology and Psychopharmacology Research Unit, Istanbul, Turkey; 2 Marmara University, Faculty of Pharmacy- Department of Pharmacognosy, Istanbul, Turkey Objective: Nigella sativa, often called black cumin, has a rich historical background since many researches showed its wide spectrum of pharmacological potential. The seeds, oil and various extracts of N. sativa have been widely used for centuries in the treatment of various ailments. N. sativa has a number of pharmacological activities such as antibacterial, antifungal, antioxidant, antiarthritic, antidiabetic, anticancer, antiinflammatory, analgesic, immunomodulatory, cardiovascular, gastroprotective, hepatoprotective, nephroprotective, antiasthmatic and anticonvulsant [1]. Moreover it has been shown to have central effects, especially anxiolytic, antidepressant actions and improving learning and memory [1,2]. Repeated administration of N. sativa oil in rats decreases serotonergic turnover and produces anxiolytic effects in open field test and elevated plus maze test [3] and suggested that a long term administration may enhance learning and memory by increasing serotonine levels in rats [1]. The results of previous experimental studies have supported the pharmacological effects of the seeds of N. sativa or major ingradient thymoquinone. Moreover N. sativa has showen to prevent lipopolysaccharideinduced depression like behavior in rats [4]. The aim of the present study was to investigate potential acute antidepressant effect of hydro-alcoholic N. sativa extract in the tail suspension test. Methods: 27 Balb/C mice (25−35 g) were divided into Control (n = 10), Imipramine (10 mg/kg, i.p., n = 5), N. sativa (250 mg/kg, i.p., n = 6), N. sativa (500 mg/kg, i.p., n = 6) groups. Imipramine and hydro-alcoholic extracts (70 ethanol: 30 water %) of N. sativa seeds were administered 30 min prior to tail suspension test. Mice were tested on open field in order to make sure that chosen doses have no effect on locomotory activity. For tail suspension test, mice were suspended in a frame 40 cm above the floor using a paper adhesive tape that placed approximately 1 cm from the tip of the tail. Time of immobility was defined as a complete lack of movement and scored during 5 minutes. The differences between groups were compared by one-way ANOVA and post hoc Dunnett’s test for statistical analyses and p values <0.05 considered statistically significant. Results: Immobility time was significantly reduced in imipramine group compared to control group (p < 0.05). N. sativa (500 mg/kg) significantly decreased the immobility time compared to control. In N. sativa (250 mg/kg) group, there was a tendency of
[1] Ahmad, A., Husain, A., Mujeeb, M., Khan, S.A., Najmi, A.K., Siddique, N.A., Damanhouri, Z.A., Anwar, F., 2013. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed 3(5), 337−52. [2] Al-Naggar, T.B., G´omez-Serranillos, M.P., Carretero, M.E., Villar, A.M., 2003. Neuropharmacological activity of Nigella sativa L. extracts. J Ethnopharmacol 88(1) 63−8. [3] Perveen, T., Haider, S., Kanwal, S., Haleem, D.J., 2009. Repeated administration of Nigella sativa decreases 5-HT turnover and produces anxiolytic effects in rats. Pak J Pharm Sci 22(2), 139–144. [4] Hosseini, M., Zakeri, S., Khoshdast, S., Yousefian, F.T., Rastegar, M., Vafaee, F., Kahdouee, S., Ghorbani, F., Rakhshandeh, H., Kazemi, S.A., 2012. The effects of Nigella sativa hydro-alcoholic extract and thymoquinone on lipopolysaccharide − induced depression like behavior in rats. J Pharm Bioallied Sci 4(3) 219−25. Disclosure statement: This abstract is financially supported by Scientific Research Project Unit of Marmara University SAG-E-120613–0233
P.2.a.014 Ketamine modulates catecholamine transmission in the bed nucleus of stria terminalis: a possible role of this region in the antidepressant effects of ketamine D. Jadzic1 ° , E.C. Carboni2 , R.C. Cadeddu1 , J.S. Samardzic3 1 University of Cagliari, Department of Biomedical Sciences, Cagliari, Italy; 2 University of Cagliari, Department of Biomedical Sciences- Neuroscience Institute- Section of Cagliari- National Research Council of Italy - CNR- Cagliari- Italy, Cagliari, Italy; 3 University of Belgrade, Institute of Pharmacology- Clinical Pharmacology and Toxicology- Medical Faculty- University of Belgrade- Belgrade- Serbia., Belgrade, Serbia Introduction: Considering that is widely accepted that depression can result from the combination of genetic and epigenetic factors, and that among the latter stress has a fundamental role [1], we identify a brain area involved in stress control, such as the bed nucleus of stria terminalis (BNST) as the target of this study. We recently showed that various antidepressants share the property of increasing dose dependently catecholamine transmission in the rats BNST [2] thus we intended to exanimate the effect of an unusual antidepressant such as ketamine. The non selective N-methyl-D-aspartate (NMDA) receptor antagonist has been suggested as a promising medication in a treatment of therapyresistant patients, evoking long-lasting effect already after infusion of a single dose [3]. Aim: Through the microdialysis technique in freely moving rats, we monitored the acute effects of ketamine on catecholamine release in the BNST, and compared those with the effects of typical and atypical antidepressants, in order to better understand the role of the BNST in antidepressant effect of ketamine. Methods: Norepinephrine (NE) and dopamine (DA) were assessed in dialysate samples by HPLC and coulometric detection