- Email: [email protected]
Antifungal activity of the extracts and fractions of six chenopodiaceaeous plant species
Abstracts / Journal of Biotechnology 136S (2008) S75–S98
S89
strongest antibacterial activity on the five test bacteria. This is the first report on the antibacterial compounds from plant A. aphylla.
compounds of these plant species by means of bioassay-guided fractionation and purification is necessary.
Acknowledgements
Acknowledgements
This work was co-financed by the National Key Technology R&D Program of China (2006BAD08A03 and 2007BAD57B02) and the Bingtuan Doctoral Foundation of China (2006JC09).
This work was co-financed by the National Key Technology R&D Program of China (2006BAD08A03 and 2007BAD57B02) and the Bingtuan Doctoral Foundation of China (2006JC09).
References
References
Du, H., Zhou, L., Li, C., Sui, P., 2007a. Recent studies on the chemistry and bioactivity of chenopodiaceous plants. Nat. Prod. Res. Dev. 19, 884–889. Du, H., Zhou, L., Tang, J., Li, C., Li, H., 2007b. Antimicrobial activity of the extracts from Anabasis aphylla L. and Kalidium foliatum (Pall.) Moq. Nat. Prod. Res. Dev. 19, 92–96. Langfied, R.D., Scarano, F.J., Heitzman, M.E., Kondo, M., Hammond, G.B., Neto, C.C., 2004. Use of a modified microplate bioassay method to investigate antibacterial activity in the Peruvian medicinal plant Peperomia galioides. J. Ethnopharmacol. 94, 279–281.
Quiroga, E.N., Sampietro, A.R., Vattuone, M.A., 2001. Screening antifungal activities of selected medicinal plants. J. Ethnopharmacol. 74, 89–96. Zhu, G.L., 1995. Origin, differentiation and geographic distribution of the Chenopodiaceae. Acta Phytotaxonomica Sin. 34, 486–504.
doi:10.1016/j.jbiotec.2008.07.200
Development and comparison of three different methods for the analysis of traditional Chinese medicine
I3-P-042
Yanming Liu ∗ , Xingya Xue, Qing Xu, Feifang Zhang, Xinmiao Liang
Antifungal activity of the extracts and fractions of six chenopodiaceaeous plant species
Multi-component Chinese Medicine Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Hongbing Yang 1,2,∗ , Hua Du 2 , Yaming Zhou 2 , Hao Liu 1 , Zhanhong Ma 2 , Chun Li 1 , Ligang Zhou 2
E-mail address: [email protected] (Y. Liu).
1
School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China 2 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China E-mail address: [email protected] (H. Yang).
Chenopodiaceous plants widely distribute in arid and salina area. There are 184 chenopodiaceous species belonging to 38 genera in China. Most of them distribute in Xinjiang, northwest of China (Zhu, 1995). Over the past decade there has been an elevated interest in searching for antimicrobial agents of plant origin, as well as in isolating and identifying antimicrobial compounds with possible use in integrated plant protection and disease management programs. In this study, six chenopodiaceous plant species (i.e. Ceratocarpus arenarius L., Halostachys caspica Bieb., Kalidium capsicum Ung.-Sternb., Petrosimonia sibirica Bunge, Salicornia europaea L. and Suaeda microphylla Pall.), which are rich in their great resources mainly distributing in Xinjiang of China (Zhu, 1995), were selected to screen their antimicrobial activity against three plant pathogenic fungi (i.e. Botrysphaeria dothidea, Fusarium oxysporum f.sp. vasinfectum and Rhizoctonia solani). The mycelial radial growth inhibition test (Quiroga et al., 2001) was used to evaluate the antifungal activity of plant extracts and different poplar fractions. Most of the plant extracts and fractions showed their strongly antifungal activity on B. dothidea which was the poplar stem blister canker pathogen in three test fungi. Results indicated that antifungal compounds mainly existed in the petroleum ether fraction, chloroform fraction and n-butanol fraction which means inhibitory compounds exhibited moderate polarity. Of all extracts and fractions, the ethanol crude extracts and different polar fractions of C. arenarius and S. europaea showed their strongest antifungal activity on test fungi and could be used as potential antifungal agents for the control of plant fungal diseases. Further investigation of antimicrobial
∗ Corresponding author at: School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China.
doi:10.1016/j.jbiotec.2008.07.201 I3-P-043
As an important resource for drug development, the traditional Chinese medicine (TCM) is a typical complex matrix. The bioactive ingredients existing in TCM are often in trace amount. Therefore, highly efficient methods are required for the separation and identification of the ingredients in it. In this work, three methods, including liquid chromatography, two-dimensional liquid chromatography (2D-LC) based on the same C18 column but different organic modifiers tetrahydrofuran and acetonitrile in the two dimensions and computer-simulation methods accomplished by Complex Sample Analysis Software System (CSASS) (Jin et al., 2008) were developed for the analysis of the chloroform extract of swertia franchetiana Smith, which is a traditional Tibetan medicine for treatment of hepatitis and cholecystitis. Through comparison of the three methods, it can be concluded that the uni-dimensional chromatography was simplest among the three ones. It has least requirement on instrument, operation and data processing. But the information obtained was the least, too. Computer-aided optimization methods may be the compromise among the three methods. Much information may be obtained by computer without interminable experiments. But the defects of it lay in its low ability for the discovery of low-abundant components, and much information about the sample may be lost. More peaks can be detected by the 2DLC method, although its operation was most complicated among the three ones. The results of the three methods can be combined and approved by each other. The pattern of the simulated chromatogram was in accord with that of one-dimensional separation under the same chromatographic condition, which validated the correctness of the prediction of the CSASS software to some degrees. It was predicted by the CSASS software that some peaks cannot be separated under any conditions in the given one-dimensional column system. The result of 2DLC separation could justify the existence of these compounds as they were well resolved.
S89
strongest antibacterial activity on the five test bacteria. This is the first report on the antibacterial compounds from plant A. aphylla.
compounds of these plant species by means of bioassay-guided fractionation and purification is necessary.
Acknowledgements
Acknowledgements
This work was co-financed by the National Key Technology R&D Program of China (2006BAD08A03 and 2007BAD57B02) and the Bingtuan Doctoral Foundation of China (2006JC09).
This work was co-financed by the National Key Technology R&D Program of China (2006BAD08A03 and 2007BAD57B02) and the Bingtuan Doctoral Foundation of China (2006JC09).
References
References
Du, H., Zhou, L., Li, C., Sui, P., 2007a. Recent studies on the chemistry and bioactivity of chenopodiaceous plants. Nat. Prod. Res. Dev. 19, 884–889. Du, H., Zhou, L., Tang, J., Li, C., Li, H., 2007b. Antimicrobial activity of the extracts from Anabasis aphylla L. and Kalidium foliatum (Pall.) Moq. Nat. Prod. Res. Dev. 19, 92–96. Langfied, R.D., Scarano, F.J., Heitzman, M.E., Kondo, M., Hammond, G.B., Neto, C.C., 2004. Use of a modified microplate bioassay method to investigate antibacterial activity in the Peruvian medicinal plant Peperomia galioides. J. Ethnopharmacol. 94, 279–281.
Quiroga, E.N., Sampietro, A.R., Vattuone, M.A., 2001. Screening antifungal activities of selected medicinal plants. J. Ethnopharmacol. 74, 89–96. Zhu, G.L., 1995. Origin, differentiation and geographic distribution of the Chenopodiaceae. Acta Phytotaxonomica Sin. 34, 486–504.
doi:10.1016/j.jbiotec.2008.07.200
Development and comparison of three different methods for the analysis of traditional Chinese medicine
I3-P-042
Yanming Liu ∗ , Xingya Xue, Qing Xu, Feifang Zhang, Xinmiao Liang
Antifungal activity of the extracts and fractions of six chenopodiaceaeous plant species
Multi-component Chinese Medicine Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Hongbing Yang 1,2,∗ , Hua Du 2 , Yaming Zhou 2 , Hao Liu 1 , Zhanhong Ma 2 , Chun Li 1 , Ligang Zhou 2
E-mail address: [email protected] (Y. Liu).
1
School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China 2 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China E-mail address: [email protected] (H. Yang).
Chenopodiaceous plants widely distribute in arid and salina area. There are 184 chenopodiaceous species belonging to 38 genera in China. Most of them distribute in Xinjiang, northwest of China (Zhu, 1995). Over the past decade there has been an elevated interest in searching for antimicrobial agents of plant origin, as well as in isolating and identifying antimicrobial compounds with possible use in integrated plant protection and disease management programs. In this study, six chenopodiaceous plant species (i.e. Ceratocarpus arenarius L., Halostachys caspica Bieb., Kalidium capsicum Ung.-Sternb., Petrosimonia sibirica Bunge, Salicornia europaea L. and Suaeda microphylla Pall.), which are rich in their great resources mainly distributing in Xinjiang of China (Zhu, 1995), were selected to screen their antimicrobial activity against three plant pathogenic fungi (i.e. Botrysphaeria dothidea, Fusarium oxysporum f.sp. vasinfectum and Rhizoctonia solani). The mycelial radial growth inhibition test (Quiroga et al., 2001) was used to evaluate the antifungal activity of plant extracts and different poplar fractions. Most of the plant extracts and fractions showed their strongly antifungal activity on B. dothidea which was the poplar stem blister canker pathogen in three test fungi. Results indicated that antifungal compounds mainly existed in the petroleum ether fraction, chloroform fraction and n-butanol fraction which means inhibitory compounds exhibited moderate polarity. Of all extracts and fractions, the ethanol crude extracts and different polar fractions of C. arenarius and S. europaea showed their strongest antifungal activity on test fungi and could be used as potential antifungal agents for the control of plant fungal diseases. Further investigation of antimicrobial
∗ Corresponding author at: School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China.
doi:10.1016/j.jbiotec.2008.07.201 I3-P-043
As an important resource for drug development, the traditional Chinese medicine (TCM) is a typical complex matrix. The bioactive ingredients existing in TCM are often in trace amount. Therefore, highly efficient methods are required for the separation and identification of the ingredients in it. In this work, three methods, including liquid chromatography, two-dimensional liquid chromatography (2D-LC) based on the same C18 column but different organic modifiers tetrahydrofuran and acetonitrile in the two dimensions and computer-simulation methods accomplished by Complex Sample Analysis Software System (CSASS) (Jin et al., 2008) were developed for the analysis of the chloroform extract of swertia franchetiana Smith, which is a traditional Tibetan medicine for treatment of hepatitis and cholecystitis. Through comparison of the three methods, it can be concluded that the uni-dimensional chromatography was simplest among the three ones. It has least requirement on instrument, operation and data processing. But the information obtained was the least, too. Computer-aided optimization methods may be the compromise among the three methods. Much information may be obtained by computer without interminable experiments. But the defects of it lay in its low ability for the discovery of low-abundant components, and much information about the sample may be lost. More peaks can be detected by the 2DLC method, although its operation was most complicated among the three ones. The results of the three methods can be combined and approved by each other. The pattern of the simulated chromatogram was in accord with that of one-dimensional separation under the same chromatographic condition, which validated the correctness of the prediction of the CSASS software to some degrees. It was predicted by the CSASS software that some peaks cannot be separated under any conditions in the given one-dimensional column system. The result of 2DLC separation could justify the existence of these compounds as they were well resolved.