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Antimicrobial Activity of Equisetum Giganteum Extract Against Clinical Strains of Candida Albicans, Escherichia Coli, And Standard Strain of Staphylococcus Aureus
ORAL AND MAXILLOFACIAL PATHOLOGY e198 Abstracts determining if biopsy is indicated and in monitoring actinic cheilitis. Material and Methods: Thirty patients with actinic cheilitis were submitted to a scrape and biopsy of the lower lip. The slides were analyzed by an experienced pathologist in a blinded study. Results: Actinic cheilitis was associated with 4 (12%) carcinomas, 11 (33%) moderate epithelial dysplasias, and 16 (49%) mild dysplasias. Cytopathology identified 3 (75%) of the four carcinomas. Considering the 11 cases with moderate dysplasia, 1 (9%) was identified as severe, 4 (36%) moderate, and 6 (55%) mild; 11 (69%) were confirmed to be mild among the 16 with mild dysplasia. Cytopathology can help in detecting atypical epithelial cells of actinic cheilitis, by indicating the need of biopsy, and as a method for monitoring patients.
PE-342 - AMYLOIDOSIS: EXPRESSION IN ORAL CAVITY. LARISSA ARAÚJO QUEIROZ, LÍVIA SOUZA PUGLIESE, LUCIANA CARVALHO, THIAGO MARCELINO SODRÉ, SAMILLY SILVA MIRANDA, MARCO ANTONIO ALVES, JENER GONÇALVES DE FARIAS. UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA. Amyloidosis refers to the extracellular deposition and progressive and irreversible accumulation of a heterogeneous group of pathogenic fibrillar proteins called amyloid. Deposits can occur locally or generally. Man, 87, reported having bilateral lesions in the mouth. His medical history included hypertension, cardiac arrhythmia, duodenal cancer, and proteinuria. Physical examination revealed multiple intraoral nodular popular lesions. The lesions were sessile, exophytic, rough, and whitish. After biopsy, histopathological and histochemical results indicated the presence of protein with conclusive evidence based on Congo red staining. To estimate systemic involvement, the patient was advised to see a nephrologist, but he died 2 months later, pending further investigation to determine if the disorder was local or systemic. Amyloidosis in the head and neck is uncommon, but when it occurs the mouth is the most commonly affected site. The nonspecific early symptoms often delay diagnosis until an advanced stage of the disease.
PE-343 - ANALYSIS OF THE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) AND FACTOR VIII IN ACTINIC CHEILITIS AND IN SQUAMOUS CELL CARCINOMA OF THE LIP. LUIS CLÁUDIO CARDOSO DOS SANTOS, LUIS CLÁUDIO CARDOSO DOS SANTOS, REBECA MOTA VASCONCELOS, TAÍSE SANTOS, GARDÊNIA MATOS PARAGUASSÚ, LUCIANA MARIA PEDREIRA RAMALHO, SILVIA REGINA REIS. FACULDADE DE ODONTOLOGIA DA UNIVERSIDADE FEDERAL DA BAHIA. Actinic cheilitis (AC) is a premalignant lesion related to chronic solar exposure of the lower lip that can undergo malignant transformation into squamous cell carcinoma (SCC). This study evaluated VEGF and factor VIII in SCC displaying AC. Material and Methods: Thirty-four samples of SCC displaying AC were analyzed, graded, and immunostained for VEGF and factor VIII using LSABTM. Hot spot areas for both markers in AC and SCC were studied. Results: SCC specimens expressed factor VIII and VEGF, including AC areas. Expression was higher compared to AC (p < 0.05). No difference was found between VEGF and factor VIII in different histological grades of dysplasia. However, well-differentiated SCC has higher expression of VEGF compared to moderate SCC (p < 0.05). Conclusion: VEGF and factor VIII did not contribute to the progression
OOOO February 2014 of AC to SCC. Further, higher expression of VEGF in welldifferentiated SCC suggests this cytokine is related to the beginning of tumorigenesis.
PE-344 - ANTIMICROBIAL ACTIVITY OF EQUISETUM GIGANTEUM EXTRACT AGAINST CLINICAL STRAINS OF CANDIDA ALBICANS, ESCHERICHIA COLI, AND STANDARD STRAIN OF STAPHYLOCOCCUS AUREUS. FELIPE ALAVARCE DE OLIVEIRA, FELIPE ALAVARCE DE OLIVEIRA, RAFAELA ALVES DA SILVA ALAVARCE, ANNE LIGIA DOKKEDAL BOSQUEIRO, VANESSA SOARES LARA. FACULDADE DE ODONTOLOGIA DE BAURUeUSP. Candida albicans adheres directly or via an intermediary layer of plaque-forming bacteria to denture base acrylic resin. Mixed candidal-bacterial biofilm is strongly associated with a high prevalence of denture stomatitis. The effects of the main medicinal plant claiming to be an antifungal agent, Equisetum giganteum, an endemic species of Latin America, might offer a natural safeguard against the development of infectious conditions. Material and Methods: This study evaluated in vitro antimicrobial activity of different concentrations of E. giganteum against strains of C. albicans SC 5314, Escherichia coli O: 124 and Staphylococcus aureus ATCC 6538, by the broth microdilution method. Results: All concentrations of the herbal extracts possessed bactericidal activity against the strains analyzed. Conclusion: E. giganteum could be considered a bioactive compound displaying antimicrobial activities that have a promising potential for use as pharmaceutical agents to treat infectious diseases, such as denture stomatitis.
PE-345 - ASSESSEMENT OF THE BINARY GRADING SYSTEM’S USEFULNESS IN POTENTIALLY MALIGNANT DISORDERS. FERNANDA CAIRES BORBA, LORENA AGUIAR SANTOS FARIA, REBECA BARROS NASCIMENTO, LIA PONTES ARRUDA PORTO, CLARISSA ARAÚJO SILVA GURGEL, JEAN NUNES DOS SANTOS, FLÁVIA CALÓ AQUINO XAVIER. UNIVERSIDADE FEDERAL DA BAHIA. Potentially malignant disorders (PMD) may precede or be associated with oral cancer. The predictive risk of PMD malignant transformation is unknown. This study evaluated the usefulness of the binary grading system of oral epithelial dysplasia (OED) in identifying PMD clinical risk profile. Study Design: Seventy-four PMD were selected from archives of the Oral Pathology Service (2002 to 2012) according to their histopathological diagnosis. Clinicopathological data (age, gender, site, smoking habit, size, and pathological grade) were collected. Twenty-nine cases were classified according to the binary grading system. Results: There were 26 (89.7%) low-risk OED and 3 (10.3%) high-risk OED. Lesion size (less than 2.0 cm) showed a trend toward being associated with low-risk OED (p ¼ 0.1, Fisher’s text). Nearly PMD classified as hyperkeratosis were significantly reclassified as low-risk OED (p ¼ 0.001). Conclusion: The biological significance of low-risk and high-risk dysplasia should be validated to support clinical practices.
PE-346 - ASSOCIATION BETWEEN MATERNAL SMOKING, GENDER, AND CLEFT LIP AND PALATE IN A BRAZILIAN POPULATION. DANIELLA REIS BARBOSA MARTELLI, LAÍSE ANGÉLICA RODRIGUES, LÍVIA MARIS PARANAÍBA, SIBELE NASCIMENTO DE AQUINO, MÁRIO SÉRGIO OLIVEIRA SWERTS, LETÍZIA MONTEIRO
PE-342 - AMYLOIDOSIS: EXPRESSION IN ORAL CAVITY. LARISSA ARAÚJO QUEIROZ, LÍVIA SOUZA PUGLIESE, LUCIANA CARVALHO, THIAGO MARCELINO SODRÉ, SAMILLY SILVA MIRANDA, MARCO ANTONIO ALVES, JENER GONÇALVES DE FARIAS. UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA. Amyloidosis refers to the extracellular deposition and progressive and irreversible accumulation of a heterogeneous group of pathogenic fibrillar proteins called amyloid. Deposits can occur locally or generally. Man, 87, reported having bilateral lesions in the mouth. His medical history included hypertension, cardiac arrhythmia, duodenal cancer, and proteinuria. Physical examination revealed multiple intraoral nodular popular lesions. The lesions were sessile, exophytic, rough, and whitish. After biopsy, histopathological and histochemical results indicated the presence of protein with conclusive evidence based on Congo red staining. To estimate systemic involvement, the patient was advised to see a nephrologist, but he died 2 months later, pending further investigation to determine if the disorder was local or systemic. Amyloidosis in the head and neck is uncommon, but when it occurs the mouth is the most commonly affected site. The nonspecific early symptoms often delay diagnosis until an advanced stage of the disease.
PE-343 - ANALYSIS OF THE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) AND FACTOR VIII IN ACTINIC CHEILITIS AND IN SQUAMOUS CELL CARCINOMA OF THE LIP. LUIS CLÁUDIO CARDOSO DOS SANTOS, LUIS CLÁUDIO CARDOSO DOS SANTOS, REBECA MOTA VASCONCELOS, TAÍSE SANTOS, GARDÊNIA MATOS PARAGUASSÚ, LUCIANA MARIA PEDREIRA RAMALHO, SILVIA REGINA REIS. FACULDADE DE ODONTOLOGIA DA UNIVERSIDADE FEDERAL DA BAHIA. Actinic cheilitis (AC) is a premalignant lesion related to chronic solar exposure of the lower lip that can undergo malignant transformation into squamous cell carcinoma (SCC). This study evaluated VEGF and factor VIII in SCC displaying AC. Material and Methods: Thirty-four samples of SCC displaying AC were analyzed, graded, and immunostained for VEGF and factor VIII using LSABTM. Hot spot areas for both markers in AC and SCC were studied. Results: SCC specimens expressed factor VIII and VEGF, including AC areas. Expression was higher compared to AC (p < 0.05). No difference was found between VEGF and factor VIII in different histological grades of dysplasia. However, well-differentiated SCC has higher expression of VEGF compared to moderate SCC (p < 0.05). Conclusion: VEGF and factor VIII did not contribute to the progression
OOOO February 2014 of AC to SCC. Further, higher expression of VEGF in welldifferentiated SCC suggests this cytokine is related to the beginning of tumorigenesis.
PE-344 - ANTIMICROBIAL ACTIVITY OF EQUISETUM GIGANTEUM EXTRACT AGAINST CLINICAL STRAINS OF CANDIDA ALBICANS, ESCHERICHIA COLI, AND STANDARD STRAIN OF STAPHYLOCOCCUS AUREUS. FELIPE ALAVARCE DE OLIVEIRA, FELIPE ALAVARCE DE OLIVEIRA, RAFAELA ALVES DA SILVA ALAVARCE, ANNE LIGIA DOKKEDAL BOSQUEIRO, VANESSA SOARES LARA. FACULDADE DE ODONTOLOGIA DE BAURUeUSP. Candida albicans adheres directly or via an intermediary layer of plaque-forming bacteria to denture base acrylic resin. Mixed candidal-bacterial biofilm is strongly associated with a high prevalence of denture stomatitis. The effects of the main medicinal plant claiming to be an antifungal agent, Equisetum giganteum, an endemic species of Latin America, might offer a natural safeguard against the development of infectious conditions. Material and Methods: This study evaluated in vitro antimicrobial activity of different concentrations of E. giganteum against strains of C. albicans SC 5314, Escherichia coli O: 124 and Staphylococcus aureus ATCC 6538, by the broth microdilution method. Results: All concentrations of the herbal extracts possessed bactericidal activity against the strains analyzed. Conclusion: E. giganteum could be considered a bioactive compound displaying antimicrobial activities that have a promising potential for use as pharmaceutical agents to treat infectious diseases, such as denture stomatitis.
PE-345 - ASSESSEMENT OF THE BINARY GRADING SYSTEM’S USEFULNESS IN POTENTIALLY MALIGNANT DISORDERS. FERNANDA CAIRES BORBA, LORENA AGUIAR SANTOS FARIA, REBECA BARROS NASCIMENTO, LIA PONTES ARRUDA PORTO, CLARISSA ARAÚJO SILVA GURGEL, JEAN NUNES DOS SANTOS, FLÁVIA CALÓ AQUINO XAVIER. UNIVERSIDADE FEDERAL DA BAHIA. Potentially malignant disorders (PMD) may precede or be associated with oral cancer. The predictive risk of PMD malignant transformation is unknown. This study evaluated the usefulness of the binary grading system of oral epithelial dysplasia (OED) in identifying PMD clinical risk profile. Study Design: Seventy-four PMD were selected from archives of the Oral Pathology Service (2002 to 2012) according to their histopathological diagnosis. Clinicopathological data (age, gender, site, smoking habit, size, and pathological grade) were collected. Twenty-nine cases were classified according to the binary grading system. Results: There were 26 (89.7%) low-risk OED and 3 (10.3%) high-risk OED. Lesion size (less than 2.0 cm) showed a trend toward being associated with low-risk OED (p ¼ 0.1, Fisher’s text). Nearly PMD classified as hyperkeratosis were significantly reclassified as low-risk OED (p ¼ 0.001). Conclusion: The biological significance of low-risk and high-risk dysplasia should be validated to support clinical practices.
PE-346 - ASSOCIATION BETWEEN MATERNAL SMOKING, GENDER, AND CLEFT LIP AND PALATE IN A BRAZILIAN POPULATION. DANIELLA REIS BARBOSA MARTELLI, LAÍSE ANGÉLICA RODRIGUES, LÍVIA MARIS PARANAÍBA, SIBELE NASCIMENTO DE AQUINO, MÁRIO SÉRGIO OLIVEIRA SWERTS, LETÍZIA MONTEIRO