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Antimycobacterial activity of medicinal plants against the causative agent of buruli ulcer: Mycobacterium ulcerans
IJMYCO 549
No. of Pages 1, Model 7
23 November 2016 International Journal of Mycobacteriology
x x x ( 2 0 1 6 ) x x x –x x x
Available at www.sciencedirect.com
ScienceDirect journal homepage: www.elsevier.com/locate/IJMYCO 6 7 3 4 5
8 9 10 11 12 13 14 15
Antimycobacterial activity of medicinal plants against the causative agent of buruli ulcer: Mycobacterium ulcerans R. Keumoe a,*, M.S. Nguembou a, F.P.V. Tsouh a,b, D.V.F. Donkeng a, D. Dize a, Y.L.R. Tchokouaha a,b,c, M.C.D. Jiatsia a, F.R.D. Youmsi a, B. Ngameni d, B.F. Fekam a a
Antimicrobial and Biocontrol Agents Unit, Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon Department of Clinical Pathology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Ghana c Institute of Medical Research and Medicinal Plants Studies, Yaounde, Cameroon d Laboratory of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon b
16 1 8
A R T I C L E I N F O
A B S T R A C T
19 20 21 22 23 24
Article history:
Objective/background: Buruli ulcer, known as necrotizing skin disease caused by Mycobac-
Received 20 September 2016
terium ulcerans, has emerged as the most prevalent mycobacteriosis after leprosy and tuber-
Received in revised form
culosis. Accordingly, it has been classified by the World Health Organization as a neglected
7 November 2016
disease with high significance in tropical areas, including Cameroon. So far, the control of
Accepted 10 November 2016
the disease relies mainly on the rifampin-streptomycin combination. Despite its efficiency,
Available online xxxx
it has shown considerable issues, including availability and side effects. Therefore, more
25 26 27 28 29 30
Keywords:
have always been of great importance in drug discovery. Therefore, the present study was
Antimycobacterial activity
initiated to assess the antimycobacterial properties of four medicinal plants against
Chemical composition
M. ulcerans.
Mycobacterium ulcerans
Methods: The methanolic and aqueous crude extracts prepared from Ficus binjamina, Ficus
Plant extract
elastica, Ficus saussureana, and Terminalia superba were screened against M. ulcerans using
effective and safer drugs are urgently warranted. For this fact, natural plant-based products
the resazurin microtiter assay method. The phytochemical screening of promising extracts was performed to reveal bioactive components that might explain the exhibited activity. Results: Out of the 24 tested extracts, 11 extracts showed promising activity with minimal inhibitory concentration ranging from 62.5 lg/mL to 250 lg/mL. The phytochemical screening revealed the presence of phenols, flavonoids, tanins, triterpenes, glucosides, and saponins. Conclusion: The obtained results further strengthened the exploitation of these extracts as potent hits in the treatment of Buruli ulcer. Meanwhile, further studies are required to fully characterize the bioactive compounds.
54 56 55 57 58
Conflicts of interest The authors have no conflicts of interest to declare.
* Corresponding author. E-mail address: [email protected] (R. Keumoe). Peer review under responsibility of Asian African Society for Mycobacteriology. http://dx.doi.org/10.1016/j.ijmyco.2016.11.004
Please cite this article in press as: R Keumoe et al. Antimycobacterial activity of medicinal plants against the causative agent of buruli ulcer: Mycobacterium ulcerans. Int. J. Mycobacteriol. (2016), http://dx.doi.org/10.1016/j.ijmyco.2016.11.004
No. of Pages 1, Model 7
23 November 2016 International Journal of Mycobacteriology
x x x ( 2 0 1 6 ) x x x –x x x
Available at www.sciencedirect.com
ScienceDirect journal homepage: www.elsevier.com/locate/IJMYCO 6 7 3 4 5
8 9 10 11 12 13 14 15
Antimycobacterial activity of medicinal plants against the causative agent of buruli ulcer: Mycobacterium ulcerans R. Keumoe a,*, M.S. Nguembou a, F.P.V. Tsouh a,b, D.V.F. Donkeng a, D. Dize a, Y.L.R. Tchokouaha a,b,c, M.C.D. Jiatsia a, F.R.D. Youmsi a, B. Ngameni d, B.F. Fekam a a
Antimicrobial and Biocontrol Agents Unit, Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon Department of Clinical Pathology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Ghana c Institute of Medical Research and Medicinal Plants Studies, Yaounde, Cameroon d Laboratory of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon b
16 1 8
A R T I C L E I N F O
A B S T R A C T
19 20 21 22 23 24
Article history:
Objective/background: Buruli ulcer, known as necrotizing skin disease caused by Mycobac-
Received 20 September 2016
terium ulcerans, has emerged as the most prevalent mycobacteriosis after leprosy and tuber-
Received in revised form
culosis. Accordingly, it has been classified by the World Health Organization as a neglected
7 November 2016
disease with high significance in tropical areas, including Cameroon. So far, the control of
Accepted 10 November 2016
the disease relies mainly on the rifampin-streptomycin combination. Despite its efficiency,
Available online xxxx
it has shown considerable issues, including availability and side effects. Therefore, more
25 26 27 28 29 30
Keywords:
have always been of great importance in drug discovery. Therefore, the present study was
Antimycobacterial activity
initiated to assess the antimycobacterial properties of four medicinal plants against
Chemical composition
M. ulcerans.
Mycobacterium ulcerans
Methods: The methanolic and aqueous crude extracts prepared from Ficus binjamina, Ficus
Plant extract
elastica, Ficus saussureana, and Terminalia superba were screened against M. ulcerans using
effective and safer drugs are urgently warranted. For this fact, natural plant-based products
the resazurin microtiter assay method. The phytochemical screening of promising extracts was performed to reveal bioactive components that might explain the exhibited activity. Results: Out of the 24 tested extracts, 11 extracts showed promising activity with minimal inhibitory concentration ranging from 62.5 lg/mL to 250 lg/mL. The phytochemical screening revealed the presence of phenols, flavonoids, tanins, triterpenes, glucosides, and saponins. Conclusion: The obtained results further strengthened the exploitation of these extracts as potent hits in the treatment of Buruli ulcer. Meanwhile, further studies are required to fully characterize the bioactive compounds.
54 56 55 57 58
Conflicts of interest The authors have no conflicts of interest to declare.
* Corresponding author. E-mail address: [email protected] (R. Keumoe). Peer review under responsibility of Asian African Society for Mycobacteriology. http://dx.doi.org/10.1016/j.ijmyco.2016.11.004
Please cite this article in press as: R Keumoe et al. Antimycobacterial activity of medicinal plants against the causative agent of buruli ulcer: Mycobacterium ulcerans. Int. J. Mycobacteriol. (2016), http://dx.doi.org/10.1016/j.ijmyco.2016.11.004