- Email: [email protected]
Antioxidant enzymes in women with dyslipidemia
Monday June 26, 2000: Poster Abstracts P:W37 Other Topics
62
Methods: The SOD and GPx activities were measured by Ransod and Ransel test respectively, both produced by Randox, LDL-C was calculated by the Friedwald equation. According to the obtained values of LDL-C, the examined hypertensive women were divided into three groups: first group (I), was composed of 22 women, LDL-C < 3.32 nunol/L; second group (II), included 19 women, LDL-C 3.32--4.1 mmol/L and the third group (III), consisted of 22 women, LDL-C > 4.1 mmol/L. 98 healthy women were taken as a control. Results: The activity of SOD was significantly decreased in women of the II (965 4- 202 U/glib), p < 0.005 and the III group (933 4- 127 U/glib), p < 0.001 in comparison with the control (1136 4- 270 U/glib). The GPx activity was statistically significant decreased in all three groups: I (28.1 45.2 U/glib), p < 0.005; II (27.0 4- 4.4. U/glib), p < 0.001 and III (27.1 4- 4.0 U/glib), p < 0.001 v.s. control (33.6 4- 9.3 U/glib). Conclusion: The results have showen that there is an interaction between LDL-C and antioxidant enzymes activity and suggested that marked decrease of these enzymes in hypertensive women may increase the risk of atherosclerosis as well as it may imply on the incidence of coronary heart disease.
relationship in TG staining of bands corresponding to serum chylomicrons. An increased VLDL-Chol was observed in type Vd subjects which were easy to distinguish from type IIb or type V. In a separate study of LDL subfractions, samples rich in smaller LDL particles significantly shifted towards the anode than those of normal samples (P < 0.001). Conclusious: This method enabled analysis of lipoprotein patterns otherwise unexpected from simple determinations of serum lipids and provides an effective technique to classify types of hypedipidemia defined by WHO. Furthermore, quantitative measurement of chylomicrons, usually difficult, proved achievable, providing an additional analysis of postrandial hyperlipidemia. Consequently, we recommend this simple and easy method for clinical evaluation of abnormalities in lipoprotein profiles. I
MoP5:W37 [ The relation between fractional removal rate of fat emulsion (K2) and postheparin plasma Iipoprotein Iipase N. Sakuma, R. Ikeuchi, G. Kimura. The Third Department of Internal
Medicine, Nagoya City University Medical School, Nagoya 467-8601, Japan Objective: We investigated the relation between the fractional removal rate
I
MoP3:W37 I Antioxidant enzymes in women with dyslipidemia G. Bosilkova 1, S. Dzhekova-Stojkova I , M. Krstevska I , L. Trpehevska2.
/Department of Medical and Experimental Biochemistry, Medical Faculty, Skopje; 2Teehno-Farm, Skopje, Macedonia Objective: The aim of our study was to investigate the relationship between serum cholesterol level and red blood cell activity of antioxidant enzymes, glutathione peroxidase (GPx) and superoxide dismutase (SOD), in women with dyslipidemia. Subjects and Methods: SOD and GPx, as well as, standard sera parameters of dyslipidemia (total cholesterol, TC, high-density lipoprotein cholesterol, HDL-C, low-density lipoprotein cholesterol, LDL-C, and trilglycerides, TG), were determined in a total number of 184 female subjects. SOD and GPx activities were determined using Randox kits, TC, HDL-C and TG were assayed by classical techniques, and LDL-C was calculated. The control group included 98 healthy women with TC < 5.17 mmol/L and HDL-C > 0.9 mmol/L. According to the values of TC and HDL-C, the rest of the subjects (n = 86) were divided in three dyslipidem)'c groups: a. TC < 5.17 mmol/L and HDL-C < 0.9 mmol/L (n = 20), b. TC 5.17-6.2 mmol/L and HDL < 0.9 mmol/L (n = 22) and c. TC > 6.2 mmol/L (n = 44). TG were less than 2.3 mmol/L in all groups. Results: The obtained values showed a statistically significant decrease of SOD and GPx activities (p < 0.05 and p < 0.025) in group b (1050 4- 155 and 27.9 -4- 11, U/glib) and in group c (1043 4- 192 and 28.4 -4- 7.4, U/glib), when compared to the controls (1132 4- 270 and 33.6 4- 9.3, U/glib). The investigated correlation between cholesterol (TC, HDL-C and LDL-C) and antioxidant enzymes was very pure in all groups. Conclusions: Our results suggest that decreased antioxidant protection may increase the risk of atherosclerosis in patients with dyslipidemia and that increased intake of antioxidants may have a role in preventing coronary heart disease.
I MoP4:W37 I A new method of lipoprotein analysis using agarose gel electrophoresis and differential staining of iipids T. Kido I , H. Kurata2, A. Matsumoto I , R. Tobiyama3, M. Kamiyama4, K. Utsunomiya2, S. Tajima2, H. Itakura I , K. Kondo4./Natl. Inst. of Health
and Nutr.; 2Jikei Univ.; :THelena Laboratories; 40chanomizu Univ., Tokyo, Japan Objective: We developed a simple and rapid method for direct detection of cholesterol (Chol) and triglyceride (TG) levels in plasma lipoprotein (LP) fractions using a combination of agarose gel electrophoresis and differential staining. The method was also required to screen a broad range of serum LP's profiles. Methods: Serum samples were collected after a 12 h fast from subjects chosen randomly and their Chol and TG levels measured. Further analysis followed ultracentrifugation and electophoresis. After ultracentrifugation, Chol and TG values of each fraction were measured enzymafically. Following agarose gel electrophoresis and differential staining of fipids using the Rapid Electrophoresis System (Rep, Helena Laboratories) TG and Chol were estimated by densitometory scanning. Results: The Chol and TG levels determined by electrophoresis correlated well with those of ultracentrifugation and further, we observed a strong linear
(K2) of an intravenously injected fat emulsion (Intralipid ®) and the lipoprotein lipase (LPL) mass and activity in postheparin plasma. Methods: The LPL mass was measured by a sandwichenzyme immunoassay system. The fractional removal rate (K2) was determined by intravenous fat emulsion tolerance test. Results: K2 was significantly correlated with the LPL mass in all subjects (n = 41, r = 0.51, p < 0.001), and in normolipidemic subjects (n = 20, r = 0.72, p < 0.001) and in the subgroup of hypertriglyceridemic subjects (n = 21, r = 0.43, p < 0.05). K2 was also significantly correlated with the LPL activity in all subjects (r = 0.40, p < 0.05), and in normolipidemic subjects (r = 0.55, p < 0.05). Conclusions: The post heparin LPL mass and activity could be indirectly estimated from the fractional removal rate of fat emulsion obtained by the fat emulsion tolerance test. This is the first study to show that kinetics of an intravenously injected fat emulsion reflect the LPL mass and activity in the postheparin plasma.
MoP6:W37 I Pseudohypertriglycerydemia in 3-year-old boy J. Kubalska, J. Szaplyko, M. Adamowicz, T. Kuryt, A. Moszczyfiska, E. Casselius, W. Gradowska, J. Taybert, J. Chmielik, E. Pronicka. The
Children's Memorial Health Institute (CMHI), Warsaw, Poland A high serum glycerol level, characteristic of glycerol kinase deficiency, is the cause of a false high concentration of serum triglycerides as determined by a standard method in which glycerol is evaluated after triglyceride hydrolysis. This pseudohypertriglyceridemia is not confirmed in fipoprotein fractions analyzed by electrophoresis and precipitation/ultracentrifuge methods. Here we present a 3-yr. old boy with pseudohypertriglyceridemia due to glycerol kinase deficiency. On the first day of life he showed respiratory and circulatory failure, muscular hypotonia, substantial metabolic acidosis and hypertriglycerydemia. In the preliminary lipids/lipoproteins profile examination a discrepancy between serum TG concentration and VLDL lipoprotein fraction was seen. This indicated a high serum glycerol level as the cause of a false serum TG rise. It was confirmed by serum glycerol determination with a specific enzymatic method, with a high signal of glycerol in the urine GC-MS profile and low glycerol kinase activity in leukocytes. All suspected hypertriglyeeridemias should be verified by serum lipoprotein fraction analysis and determination of glycerol in the serum and urine.
l MoP7:W37 I Enhance ofchylomicron-like emulsion lipolysis in patients with heart failure C.G. Vinagre, A.C. Sposito, N. Stolf, E. Bocchi, R.E Am~mcio, J.A.E Ramires, R.C. Maranh~o. Heart Institute (lnCor), University of S~o
Paulo Medical School, Brazil Objective: Evaluate the chylomicron metabolism in patients with heart failure. Methods: We studied 14 patients with heart failure (HF) and coronary artery disease (CAD), 10 patients with HF without CAD, 56 patients without HF but with CAD and 34 subjects without HF or CAD. A ehylomicron-like emulsion labeled with 3H-Triolein (TO) and 14C-Cholesteryl Oleate (CO) was injected intravenously in bolus after a 12-hour fast and blood samples were collected in predetermined intervals during 60 minutes to determine the fractional clearance rate (FCR) of the labels.
Xllth International Symposium on Atherosclerosis, Stockholm, Sweden, June 25-29, 2000
62
Methods: The SOD and GPx activities were measured by Ransod and Ransel test respectively, both produced by Randox, LDL-C was calculated by the Friedwald equation. According to the obtained values of LDL-C, the examined hypertensive women were divided into three groups: first group (I), was composed of 22 women, LDL-C < 3.32 nunol/L; second group (II), included 19 women, LDL-C 3.32--4.1 mmol/L and the third group (III), consisted of 22 women, LDL-C > 4.1 mmol/L. 98 healthy women were taken as a control. Results: The activity of SOD was significantly decreased in women of the II (965 4- 202 U/glib), p < 0.005 and the III group (933 4- 127 U/glib), p < 0.001 in comparison with the control (1136 4- 270 U/glib). The GPx activity was statistically significant decreased in all three groups: I (28.1 45.2 U/glib), p < 0.005; II (27.0 4- 4.4. U/glib), p < 0.001 and III (27.1 4- 4.0 U/glib), p < 0.001 v.s. control (33.6 4- 9.3 U/glib). Conclusion: The results have showen that there is an interaction between LDL-C and antioxidant enzymes activity and suggested that marked decrease of these enzymes in hypertensive women may increase the risk of atherosclerosis as well as it may imply on the incidence of coronary heart disease.
relationship in TG staining of bands corresponding to serum chylomicrons. An increased VLDL-Chol was observed in type Vd subjects which were easy to distinguish from type IIb or type V. In a separate study of LDL subfractions, samples rich in smaller LDL particles significantly shifted towards the anode than those of normal samples (P < 0.001). Conclusious: This method enabled analysis of lipoprotein patterns otherwise unexpected from simple determinations of serum lipids and provides an effective technique to classify types of hypedipidemia defined by WHO. Furthermore, quantitative measurement of chylomicrons, usually difficult, proved achievable, providing an additional analysis of postrandial hyperlipidemia. Consequently, we recommend this simple and easy method for clinical evaluation of abnormalities in lipoprotein profiles. I
MoP5:W37 [ The relation between fractional removal rate of fat emulsion (K2) and postheparin plasma Iipoprotein Iipase N. Sakuma, R. Ikeuchi, G. Kimura. The Third Department of Internal
Medicine, Nagoya City University Medical School, Nagoya 467-8601, Japan Objective: We investigated the relation between the fractional removal rate
I
MoP3:W37 I Antioxidant enzymes in women with dyslipidemia G. Bosilkova 1, S. Dzhekova-Stojkova I , M. Krstevska I , L. Trpehevska2.
/Department of Medical and Experimental Biochemistry, Medical Faculty, Skopje; 2Teehno-Farm, Skopje, Macedonia Objective: The aim of our study was to investigate the relationship between serum cholesterol level and red blood cell activity of antioxidant enzymes, glutathione peroxidase (GPx) and superoxide dismutase (SOD), in women with dyslipidemia. Subjects and Methods: SOD and GPx, as well as, standard sera parameters of dyslipidemia (total cholesterol, TC, high-density lipoprotein cholesterol, HDL-C, low-density lipoprotein cholesterol, LDL-C, and trilglycerides, TG), were determined in a total number of 184 female subjects. SOD and GPx activities were determined using Randox kits, TC, HDL-C and TG were assayed by classical techniques, and LDL-C was calculated. The control group included 98 healthy women with TC < 5.17 mmol/L and HDL-C > 0.9 mmol/L. According to the values of TC and HDL-C, the rest of the subjects (n = 86) were divided in three dyslipidem)'c groups: a. TC < 5.17 mmol/L and HDL-C < 0.9 mmol/L (n = 20), b. TC 5.17-6.2 mmol/L and HDL < 0.9 mmol/L (n = 22) and c. TC > 6.2 mmol/L (n = 44). TG were less than 2.3 mmol/L in all groups. Results: The obtained values showed a statistically significant decrease of SOD and GPx activities (p < 0.05 and p < 0.025) in group b (1050 4- 155 and 27.9 -4- 11, U/glib) and in group c (1043 4- 192 and 28.4 -4- 7.4, U/glib), when compared to the controls (1132 4- 270 and 33.6 4- 9.3, U/glib). The investigated correlation between cholesterol (TC, HDL-C and LDL-C) and antioxidant enzymes was very pure in all groups. Conclusions: Our results suggest that decreased antioxidant protection may increase the risk of atherosclerosis in patients with dyslipidemia and that increased intake of antioxidants may have a role in preventing coronary heart disease.
I MoP4:W37 I A new method of lipoprotein analysis using agarose gel electrophoresis and differential staining of iipids T. Kido I , H. Kurata2, A. Matsumoto I , R. Tobiyama3, M. Kamiyama4, K. Utsunomiya2, S. Tajima2, H. Itakura I , K. Kondo4./Natl. Inst. of Health
and Nutr.; 2Jikei Univ.; :THelena Laboratories; 40chanomizu Univ., Tokyo, Japan Objective: We developed a simple and rapid method for direct detection of cholesterol (Chol) and triglyceride (TG) levels in plasma lipoprotein (LP) fractions using a combination of agarose gel electrophoresis and differential staining. The method was also required to screen a broad range of serum LP's profiles. Methods: Serum samples were collected after a 12 h fast from subjects chosen randomly and their Chol and TG levels measured. Further analysis followed ultracentrifugation and electophoresis. After ultracentrifugation, Chol and TG values of each fraction were measured enzymafically. Following agarose gel electrophoresis and differential staining of fipids using the Rapid Electrophoresis System (Rep, Helena Laboratories) TG and Chol were estimated by densitometory scanning. Results: The Chol and TG levels determined by electrophoresis correlated well with those of ultracentrifugation and further, we observed a strong linear
(K2) of an intravenously injected fat emulsion (Intralipid ®) and the lipoprotein lipase (LPL) mass and activity in postheparin plasma. Methods: The LPL mass was measured by a sandwichenzyme immunoassay system. The fractional removal rate (K2) was determined by intravenous fat emulsion tolerance test. Results: K2 was significantly correlated with the LPL mass in all subjects (n = 41, r = 0.51, p < 0.001), and in normolipidemic subjects (n = 20, r = 0.72, p < 0.001) and in the subgroup of hypertriglyceridemic subjects (n = 21, r = 0.43, p < 0.05). K2 was also significantly correlated with the LPL activity in all subjects (r = 0.40, p < 0.05), and in normolipidemic subjects (r = 0.55, p < 0.05). Conclusions: The post heparin LPL mass and activity could be indirectly estimated from the fractional removal rate of fat emulsion obtained by the fat emulsion tolerance test. This is the first study to show that kinetics of an intravenously injected fat emulsion reflect the LPL mass and activity in the postheparin plasma.
MoP6:W37 I Pseudohypertriglycerydemia in 3-year-old boy J. Kubalska, J. Szaplyko, M. Adamowicz, T. Kuryt, A. Moszczyfiska, E. Casselius, W. Gradowska, J. Taybert, J. Chmielik, E. Pronicka. The
Children's Memorial Health Institute (CMHI), Warsaw, Poland A high serum glycerol level, characteristic of glycerol kinase deficiency, is the cause of a false high concentration of serum triglycerides as determined by a standard method in which glycerol is evaluated after triglyceride hydrolysis. This pseudohypertriglyceridemia is not confirmed in fipoprotein fractions analyzed by electrophoresis and precipitation/ultracentrifuge methods. Here we present a 3-yr. old boy with pseudohypertriglyceridemia due to glycerol kinase deficiency. On the first day of life he showed respiratory and circulatory failure, muscular hypotonia, substantial metabolic acidosis and hypertriglycerydemia. In the preliminary lipids/lipoproteins profile examination a discrepancy between serum TG concentration and VLDL lipoprotein fraction was seen. This indicated a high serum glycerol level as the cause of a false serum TG rise. It was confirmed by serum glycerol determination with a specific enzymatic method, with a high signal of glycerol in the urine GC-MS profile and low glycerol kinase activity in leukocytes. All suspected hypertriglyeeridemias should be verified by serum lipoprotein fraction analysis and determination of glycerol in the serum and urine.
l MoP7:W37 I Enhance ofchylomicron-like emulsion lipolysis in patients with heart failure C.G. Vinagre, A.C. Sposito, N. Stolf, E. Bocchi, R.E Am~mcio, J.A.E Ramires, R.C. Maranh~o. Heart Institute (lnCor), University of S~o
Paulo Medical School, Brazil Objective: Evaluate the chylomicron metabolism in patients with heart failure. Methods: We studied 14 patients with heart failure (HF) and coronary artery disease (CAD), 10 patients with HF without CAD, 56 patients without HF but with CAD and 34 subjects without HF or CAD. A ehylomicron-like emulsion labeled with 3H-Triolein (TO) and 14C-Cholesteryl Oleate (CO) was injected intravenously in bolus after a 12-hour fast and blood samples were collected in predetermined intervals during 60 minutes to determine the fractional clearance rate (FCR) of the labels.
Xllth International Symposium on Atherosclerosis, Stockholm, Sweden, June 25-29, 2000