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Antioxidant, nitric oxide scavenging and malondialdehyde scavenging activity of essential oils from different chemotypes of Zataria multiflora
Abstracts
cerides (p= 0.006 in Chlorella and p = 0.004 in atorvastatin group) in both groups. No significant change in serum high-density lipoprotein cholesterol levels was observed in any of the groups. Serum aspartate aminotransferase levels were raised in both Chlorella (p= 0.034) and atorvastatin (p= 0.002) groups, whereas alkaline phosphatase was only elevated in the Chlorella group (p= 0.028). In comparison with baseline values, no significant change was observed in serum levels of alanine aminotransferase, creatine phosphokinase, creatinine, blood urea nitrogen and fasting blood sugar. Conclusion: Addition of C. vulgaris to atorvastatin therapy for 8 weeks does not appear to be associated with an improved control of serum lipid profile. Keywords: Chlorella vulgaris, Dyslipidemia, Lipid profile, Atorvastatin, Clinical trial doi:10.1016/j.clinbiochem.2011.08.840
Poster – [A-10-606-1] Antioxidant, nitric oxide scavenging and malondialdehyde scavenging activity of essential oils from different chemotypes of Zataria multiflora Parastoo Karinian, Gholamreza Kavoosi Shiraz, Iran E-mail addresses: [email protected] (P. Karinian), [email protected] (G. Kavoosi) In this study the antioxidant, nitric oxide (NO) scavenging, and malodialdehyde(MDA) scavenging activity of different Zataria moltiflora (ZM) essential oils (EOs) was investigated. The main components are; ZM1(carvacrol, p-cymene), ZM2(carvacrol, p-cymene), ZM3(carvacrol, p-cymene), ZM4(linalool), ZM5(carvacrol, p-cymene, thymol), ZM6(thymol, carvacrol, p-cymene, γ-terpienene), ZM7 (thymol, p-cymene, γ-terpienene), ZM8(carvacrol, linalool, p-cymene, thymol). The antioxidant capacity was estimated 863 ± 55, 619 ±27, 876 ± 32, 38 ± 9, 649 ± 50, 595 ± 40, 696 ± 41, 618 ± 9 μg ascorbic acid equivalents per mL for ZM1 to ZM8 respectively. The NO scavenging was estimated 54 ± 1.2, 50 ± 1.4, 63 ± 1, 0.60 ± 0.1, 53 ±0.7, 53 ± 1.5, 38 ± 1.1, 46.5 ± 3 μg ascorbic acid equivalents per mL for ZM1 to ZM8 respectively. The MDA scavenging was estimated 19 ± 1, 9 ± 1, 24 ± 1, 1.6 ± 0.6, 12 ± 1, 11.7 ± 1, 10 ± 1, 12.5 ± 1.3 μg ascorbic acid equivalents per mL for ZM1 to ZM8 respectively. Among these EO ZM3 with carvacrol and p-cymene had higher antioxidant, NO scavenging and MDA scavenging. Keywords: Zataria moltiflora, Essential oils, antioxidant activity, Nitric oxide scavenging, Malodialdehyde scavenging doi:10.1016/j.clinbiochem.2011.08.841
Poster – [A-10-634-4] Comparison of antifungal effects of extracts of marigold, cinnamon, garlic and thyme on candida albicans Hassan Moghima, Ghorban Ali Shahabib a Mycolgy and Parasitilogy Dept., Medical School, Shahrekord Univ. of Medical Sciences, Shahrekord, Iran b Imunology Dept., Medical School, Shahrekord Univ. of Medical Sciences, Shahrekord, Iran E-mail addresses: [email protected] (H. Moghim), [email protected] (G.A. Shahabi) Introduction: The increasing prevalence candidiasis followed increased antifungal drugs for the treatment and prevention leading to
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some species of candida resistance to the drug. Increasing usage of medical plants, for medical treatment made for this branch of complementary medicine a unique place. This study compared antifungal effect of marigold, cinnamon, garlic and thyme on candida albicans. After preparing fresh herbs, by percolation method was had made hydroalcohlic extract and then was diluted to zero mg/ml as control dilution were prepared of the desired plants. The series of tubes culture method was used and the number of colonies grown on candida albicans counts and the MIC and MFC Were determined. MIC50 culture contains garlic. Cinnamon, marigold and thyme, was respectively, 44/32, 4/41, 9/42 and 0. And there was a significant difference between the four groups (p
Poster – [A-10-642-1] Study of Chemopreventive activity of ethanolic extract of Ferulago angulata leaves Shafagh Heidari, Hassan Akrami, Hamid Mahdiuni Department of Biology, Faculty of Science, Razi University, Kermanshah, I.R. of Iran E-mail addresses: [email protected] (S. Heidari), [email protected] (H. Akrami), [email protected] (H. Mahdiuni) Introduction: Ferulago angulata known as Chavir in Iran is a perennial shrub at altitudes of 1900–3200 m above sea level and it grows to 60–150 cm height. We postulated that Chavir extract may be a good candidate for Inhibition of growth and induction of apoptosis in cancer cells. The aim of our study was to evaluate the potential of Ferulago angulata (Apiaceae) to induce apoptosis in order to determine its probable anticancer properties. Methods: The plant was collected from mountains of Kermanshah in the west of Iran. The cytotoxic effects of the ethanolic extract of Chavir leaves were performed with Trypan blue exclusion test and Neutral Red Uptake assay at different concentrations. For the detection of apoptotic cells in situ, we used AnnexinV-PI double staining assay. Results: Trypan blue exclusion test and Neutral Red assays showed that count of dead cells is increased in parallel with increasing extract concentration with IC50 value of 80 μg/ml. At the concentrations of 40 to 120 μg/ml, the ethanolic extract of Ferulago angulata significantly inhibited proliferation and induced apoptosis in AGS cells. Conclusion: Our Results showed that Ferulago angulata extract leaves have remarkable antiproliferative and proapoptotic effects on AGS cell line. This is the first study that suggests significant Chemopreventive effects of these ethanolic extract leaves against cancer cells. Further studies will be needed to demonstrate more details about biochemical and biological evaluation of this extract. Keywords: Ferulago angulata, Anti-proliferative, Apoptosis, AnnexinV doi:10.1016/j.clinbiochem.2011.08.843
cerides (p= 0.006 in Chlorella and p = 0.004 in atorvastatin group) in both groups. No significant change in serum high-density lipoprotein cholesterol levels was observed in any of the groups. Serum aspartate aminotransferase levels were raised in both Chlorella (p= 0.034) and atorvastatin (p= 0.002) groups, whereas alkaline phosphatase was only elevated in the Chlorella group (p= 0.028). In comparison with baseline values, no significant change was observed in serum levels of alanine aminotransferase, creatine phosphokinase, creatinine, blood urea nitrogen and fasting blood sugar. Conclusion: Addition of C. vulgaris to atorvastatin therapy for 8 weeks does not appear to be associated with an improved control of serum lipid profile. Keywords: Chlorella vulgaris, Dyslipidemia, Lipid profile, Atorvastatin, Clinical trial doi:10.1016/j.clinbiochem.2011.08.840
Poster – [A-10-606-1] Antioxidant, nitric oxide scavenging and malondialdehyde scavenging activity of essential oils from different chemotypes of Zataria multiflora Parastoo Karinian, Gholamreza Kavoosi Shiraz, Iran E-mail addresses: [email protected] (P. Karinian), [email protected] (G. Kavoosi) In this study the antioxidant, nitric oxide (NO) scavenging, and malodialdehyde(MDA) scavenging activity of different Zataria moltiflora (ZM) essential oils (EOs) was investigated. The main components are; ZM1(carvacrol, p-cymene), ZM2(carvacrol, p-cymene), ZM3(carvacrol, p-cymene), ZM4(linalool), ZM5(carvacrol, p-cymene, thymol), ZM6(thymol, carvacrol, p-cymene, γ-terpienene), ZM7 (thymol, p-cymene, γ-terpienene), ZM8(carvacrol, linalool, p-cymene, thymol). The antioxidant capacity was estimated 863 ± 55, 619 ±27, 876 ± 32, 38 ± 9, 649 ± 50, 595 ± 40, 696 ± 41, 618 ± 9 μg ascorbic acid equivalents per mL for ZM1 to ZM8 respectively. The NO scavenging was estimated 54 ± 1.2, 50 ± 1.4, 63 ± 1, 0.60 ± 0.1, 53 ±0.7, 53 ± 1.5, 38 ± 1.1, 46.5 ± 3 μg ascorbic acid equivalents per mL for ZM1 to ZM8 respectively. The MDA scavenging was estimated 19 ± 1, 9 ± 1, 24 ± 1, 1.6 ± 0.6, 12 ± 1, 11.7 ± 1, 10 ± 1, 12.5 ± 1.3 μg ascorbic acid equivalents per mL for ZM1 to ZM8 respectively. Among these EO ZM3 with carvacrol and p-cymene had higher antioxidant, NO scavenging and MDA scavenging. Keywords: Zataria moltiflora, Essential oils, antioxidant activity, Nitric oxide scavenging, Malodialdehyde scavenging doi:10.1016/j.clinbiochem.2011.08.841
Poster – [A-10-634-4] Comparison of antifungal effects of extracts of marigold, cinnamon, garlic and thyme on candida albicans Hassan Moghima, Ghorban Ali Shahabib a Mycolgy and Parasitilogy Dept., Medical School, Shahrekord Univ. of Medical Sciences, Shahrekord, Iran b Imunology Dept., Medical School, Shahrekord Univ. of Medical Sciences, Shahrekord, Iran E-mail addresses: [email protected] (H. Moghim), [email protected] (G.A. Shahabi) Introduction: The increasing prevalence candidiasis followed increased antifungal drugs for the treatment and prevention leading to
S339
some species of candida resistance to the drug. Increasing usage of medical plants, for medical treatment made for this branch of complementary medicine a unique place. This study compared antifungal effect of marigold, cinnamon, garlic and thyme on candida albicans. After preparing fresh herbs, by percolation method was had made hydroalcohlic extract and then was diluted to zero mg/ml as control dilution were prepared of the desired plants. The series of tubes culture method was used and the number of colonies grown on candida albicans counts and the MIC and MFC Were determined. MIC50 culture contains garlic. Cinnamon, marigold and thyme, was respectively, 44/32, 4/41, 9/42 and 0. And there was a significant difference between the four groups (p
Poster – [A-10-642-1] Study of Chemopreventive activity of ethanolic extract of Ferulago angulata leaves Shafagh Heidari, Hassan Akrami, Hamid Mahdiuni Department of Biology, Faculty of Science, Razi University, Kermanshah, I.R. of Iran E-mail addresses: [email protected] (S. Heidari), [email protected] (H. Akrami), [email protected] (H. Mahdiuni) Introduction: Ferulago angulata known as Chavir in Iran is a perennial shrub at altitudes of 1900–3200 m above sea level and it grows to 60–150 cm height. We postulated that Chavir extract may be a good candidate for Inhibition of growth and induction of apoptosis in cancer cells. The aim of our study was to evaluate the potential of Ferulago angulata (Apiaceae) to induce apoptosis in order to determine its probable anticancer properties. Methods: The plant was collected from mountains of Kermanshah in the west of Iran. The cytotoxic effects of the ethanolic extract of Chavir leaves were performed with Trypan blue exclusion test and Neutral Red Uptake assay at different concentrations. For the detection of apoptotic cells in situ, we used AnnexinV-PI double staining assay. Results: Trypan blue exclusion test and Neutral Red assays showed that count of dead cells is increased in parallel with increasing extract concentration with IC50 value of 80 μg/ml. At the concentrations of 40 to 120 μg/ml, the ethanolic extract of Ferulago angulata significantly inhibited proliferation and induced apoptosis in AGS cells. Conclusion: Our Results showed that Ferulago angulata extract leaves have remarkable antiproliferative and proapoptotic effects on AGS cell line. This is the first study that suggests significant Chemopreventive effects of these ethanolic extract leaves against cancer cells. Further studies will be needed to demonstrate more details about biochemical and biological evaluation of this extract. Keywords: Ferulago angulata, Anti-proliferative, Apoptosis, AnnexinV doi:10.1016/j.clinbiochem.2011.08.843