Appendix I Published automated enzyme assays

APPENDIX I

Published automated enzyme assays

In some of the references in this appendix full details are given including preparation and dilution of reagents. In others the systems are presented in a more generalized way. In view of the absence of a comprehensive index of automated assays, both types of paper have been included. In some cases, the assay has been designed to estimate a substrate, rather than the enzyme. In such cases the substrate is given in brackets after the enzyme (e.g. Glucose oxidase (glucose) Hill & Kessler 1961). Enzyme assayed

References

Abou-Donia and Menzel 1967; Groff et al. 1966; Serrone et al. 1965 Acetyl thiocholinesterase Humison and Wright 1967 Acid phosphatase Bodansky and Schwartz 1961; Chersi et al. 1967; Green et al. 1966, 1967; Klein and Auerbach 1966; Klein et al. 1965, 1966; Leighton et al. 1968; Schuel and Anderson 1964; Stein and Lewis 1966; Tappel 1964 Alcohol dehydrogenase Roodyn 1965b; Schwartz et al. 1961 Alcohol dehydrogenase (alcohol) Syed 1967, 1968 Aldolase Kaldor and Schiavone (1968) Alkaline phosphatase Axelsson et al. 1965; Birkett et al. 1967; Coleman and Stroje 1965; Comfort and Campbell 1966; Cooke and Patston 1961; Everard and Seymour 1963; Fishman and Gosh 1967; Green et al. 1966; Hill et al. 1968; Horne et Acetyl cholinesterase

168

PUBLICATIONS

Enzyme assayed

Amidases Amido transferase Amino acid decarboxylase L Amino acid oxidase (L amino acids) D Amino acid oxidase Amylase

Aromatic acid decarboxylase Aromatic ketolenol tautomerase ATPase ATPase (Na+, K+ activated) Catalase Cathepsin Ceruloplasmin Cholinesterase Cholinesterase (organic pesticides) Citrate cleavage enzyme Creatine phosphokinase Cytochrome oxidase DOPA decarboxylase Ferricyanide reductases Ficin Formylase Fructose aldolase Galactosidase p-Galactosidase o-Galactosidase o-Galactosidase p-Galac tosidase

169

References al. 1968; Hviid 1967; Keay and Trew 1964; Klein and Kaufman 1967; Marrack and Hall 1965; Marsh et al. 1959; Morgenstern et al. 1965c,d; Roos 1965,1966; Sterlinget al. 1964; Tietz and Green I964 Lenard et al. 1965 Pitot et al. 1966 Scheuerbrandt 1965 Van Dyke and Szustkiewicz 1969 Leighton et al. 1968 Cadmus 1966; Chariot et al. 1966; Scheidt 1964; Strumeyer and Romano 1966; Wilding 1963 Scheuerbrandt 1965 Pitot et al. 1966 Stein et al. 1965 Kline et al. 1968 Lamy et al. 1967; Leighton et al. 1968; Pitot et al. 1966 Tappel 1968 Wilson et al. 1967 Gage and Litchfield 1966; Levine et al. 1965; Miwa et al. 1967; Winter 1960a Winter 1960b Pitot et al. 1966 Fleischer 1967; Siege1 and Cohen 1966; Willis et al. 1967 Schuel et al. 1964 Caniier and Gonnard 1966,1968 Roodyn 1965c Tappel 1968 Pitot et al. 1966 Pitot et al. 1966 Tappel 1964 Tappel and Beck 1967 Beck and Tappel 1967 Tappel and Beck 1967 Tappel and Beck 1967 Subiecl index P. 218

170 Enzyme assayed Glucokinase Glucose oxidase (glucose)

AUTOMATED ENZYME ASSAYS

References

Pitot and Pries 1964; Pitot et a1 1966, 1968 Cramp 1967; Getchell et al. 1964; Hill and Kessler 1961; Kawerau 1966; Logan and Haight 1965; Robin and Saifer 1965; Rosevaer et al. 1969; SaiferandRobin 1965;Tammesand Nordschow 1968 C lucose-6-phosphatase Leighton et al. 1968; Tappel 1964 Glucose-6-phosphate dehydrogHoober and Bernstein 1964; Miwaetal. 1967; Pitot et al. 1966, 1968 enase Glucuronidase Tappel 1964; Tappel and Beck 1967 Glutamate decarboxylase Camier and Gonnard 1966,1968; Leclerc 1967 Glutamate-oxaloacetate transAxelsson et al. 1965; Fingerhut et al. 1962; aminase (SGOT) Levine and Hill 1965; Moore and Sax 1969; Morgenstern et al. 1966b, 1967; Passen and Gennaro 1966; Schaffert et al. 1964; Schwartz et al. 1961; Trinder and Kirkland 1965; Van den Bossche 1965 Clutamate-pyruvate transaminase Axelsson et al. 1965; Levine and Hill 1965; (SGPT) SchaRert et al. 1964; Trinder and Kirkland 1965 Glutaniinase Pitot et al. 1966 Glutamine dehydrogenase Pitot et al. 1966 Glutamine synthetase Pitot et al. 1965 Glutathione reductase Kauppinen and Gref 1966 Glycerokinase Pitot et al. 1966 Guanase Nyssen and Dorche 1968 Hexokinase-glucose-6-phosphate Schersten and Tibbling 1968 dehydrogenase (glucose) Histidase Pitot and Pries 1964; Pitot et al. 1966 Histidine a-oxoglutarate Pitot et al. 1966 transaminase Hydroxybutyrate dehydrogenase Dube et al. 1968; Strandjord and Clayson 1966a L a-hydroxy acid oxidase Leighton et al. 1968 Lactate dehydrogenase (NADBerry and Walli 1966; Brooks and Olken 1965; linked) Capps et al. 1966; Dube et al. 1968; Hicks and Updike 1965; Hochella and Weinhouse 1965a, b; Hoober and Bernstein 1964; Houghton 1964; Levy et al. 1965; Morgenstern et al. 1965a, b, 1966a; Opher et al. 1966; Passen

PUBLICATIONS

Enzyme assayed

171

References

and Gennaro 1966; Pitot et al. 1966; Posen et al. 1967; Schwartz et al. 1961 ; Strandjord and Clayson I966a Lactate dehydrogenase (lactate) Hochella and Weinhouse 196% Lactate dehydrogenase (pyruvate) Cramp 1968; Minaire et al. 1966 D( -)-Lactate dehydrogenase Roodyn 1967a, b, 1969; Roodyn and Maroudas 1968 ~ ( )-Lactate -t ferricyanide Roodyn 1967a, b, 1969; Roodyn and Maroureductase das 1968 Leucine aminopeptidase Ratliff et al. 1966 Lysine decarboxylase (lysine) Schaiberger and Ferrari 1960 Lysozynie Burrows 1966; Jolles et al. 1967 Malate dehydrogenase Pitot et al. 1966; Roodyn 1965b Malic enzyme Pitot et al. 1966 Hill and Cowart 1966 Mutarotase B-N-acetyl-glucosaminidase Tappel and Beck 1967 Roodyn 1967a, b, 1969; Roodyn and MarouNADH dehydrogenase das 1968 Roodyn 1964, 1965b, c NAD-linked dehydrogenases NADPH dehydrogenase Roodyn 1969 Hill and Sarnmons 1965,1966 5’-Nucleotidase Ornithine carbanioyl transferase Girard et al. 1963; Strandjord and Clayson 1966b Pitot et al. 1965, 1966 Ornithine transaminase Oxidases Roodyn 1964 Papain Tappel 1938 Pepsin Vatier et al. 1966 Peptidases Lenard et al. 1965 Phosphatases Roodyn 1965c Phosphodiesterase Chersi et al. 1967 Phosphogl ucomu tase Hopkinson and Lewis 1967; Hoober and Bernstein 1964 6-Phosphogluconate dehydrogenase Cameron and Weg 1964; Pitot et al. 1968 Phosphohexoisomerase Schwartz et al. 1960b Polynucleotide phosphorylase Burns and Lazer 1965 Proteolytic enzymes Hazen et al. 1965; Heinicke et al. 1967 Pyruvate kinase Bigley et al. 1968 Rennin Nash et al. 1967; Skeggs et al. 1968 Barrera et al. 1969; Mundry 1965; Pitot et al. Ribonuclease 1966 SubiccI index p, 618

172

AUTOMATED ENZYME ASSAYS

Enzyme assayed

References

Serine dehydrase Succinate dehydrogenase

Pitot et al. 1966 Roodyn 1967a, b, 1969; Roodyn and Maroudas 1968 Tappel 1964; Tappel and Beck 1967 Clarke and Nicklas 1966 Stevens et al. 1967 Girard et al. 1966 a, b, c Pitot et al. 1966, 1968

Sulphatase Transglutaminase Transketolase Trypsin Tyrosine a-0x0-glutarate transaminase Tyrosine decarboxylase Tryptophan pyrrolase Tyrosine decarboxylase Urease (urea) Uricase (uric acid) Urocanase

Carnier and Gonnard 1968; Scheuerbrandt 1965 Pitot et al. 1966 Camier and Gonnard 1966 Wilson 1966 Barron and Bouley 1965 Pitot et al. 1966