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Are fluctuation tests more sensitive than plate tests?
237 and 4 NQO. "Committee 17" has given a rough estimate for NaNO: as 15 mg/ kg b o d y weight. Assuming a uniform distribution of the applied chemical within the organism, our values elevated to this scale will be 1.5 mg/kg. Considering the large uncertainties in this assumption as well as differences in the period of chemical action, the correlation obtained is encouraging.
151 McAthey, P., and B.J. Kilbey, Department of Animal Genetics, University of Edinburgh, West Mains Road, Edinburgh EH9 3JN (Scotland) Continuous cultures: a tool for studying mutagenesis in eukaryotes
Forward mutation to resistance to the 12,13-epoxytrichothecene trichodermin fulfils the main criteria necessary for use in mutational studies on continuous cultures of the microbial eukaryote, Schizosaccharomyces pombe (McAthey and Kilbey, Biol. Zbl., 95 (1976) 415). The kinetics of spontaneous mutation accumulation have been found to depend upon the nutrilite used to limit the growth of the population. Under conditions of glucose-limitation mutation accumulation is directly proportional to the rate of cell division, while under amino acid starvation it becomes proportional to chronological time. Possible explanations for these observations will be discussed.
152 MacDonald, D.J., and R.D. Callander, Mutation Group, Institute of Animal Genetics, Edinburgh University, West Mains Road, Edinburgh (Scotland) Are fluctuation tests more sensitive than plate tests? It has been claimed that the Ryan modification of the fluctuation test is a more sensitive means of detecting induced mutation than conventional plate tests. We have used strains of E. coIi and Salmonella typhimurium to compare levels of mutation, induced b y a number of known mutagens, in a fluctuation test and in a plate test in which growth occurs on the plate in the presence of the mutagen. The results suggest that, under the right conditions, the plate test is as sensitive as the fluctuation test, and furthermore has some obvious advantages of speed and economy.
153 MacMillan, S., and Margaret Fox, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX (England) The measurement o f induced mutation frequencies in V79 Chinese hamster cells: a reappraisal o f methodology
151 McAthey, P., and B.J. Kilbey, Department of Animal Genetics, University of Edinburgh, West Mains Road, Edinburgh EH9 3JN (Scotland) Continuous cultures: a tool for studying mutagenesis in eukaryotes
Forward mutation to resistance to the 12,13-epoxytrichothecene trichodermin fulfils the main criteria necessary for use in mutational studies on continuous cultures of the microbial eukaryote, Schizosaccharomyces pombe (McAthey and Kilbey, Biol. Zbl., 95 (1976) 415). The kinetics of spontaneous mutation accumulation have been found to depend upon the nutrilite used to limit the growth of the population. Under conditions of glucose-limitation mutation accumulation is directly proportional to the rate of cell division, while under amino acid starvation it becomes proportional to chronological time. Possible explanations for these observations will be discussed.
152 MacDonald, D.J., and R.D. Callander, Mutation Group, Institute of Animal Genetics, Edinburgh University, West Mains Road, Edinburgh (Scotland) Are fluctuation tests more sensitive than plate tests? It has been claimed that the Ryan modification of the fluctuation test is a more sensitive means of detecting induced mutation than conventional plate tests. We have used strains of E. coIi and Salmonella typhimurium to compare levels of mutation, induced b y a number of known mutagens, in a fluctuation test and in a plate test in which growth occurs on the plate in the presence of the mutagen. The results suggest that, under the right conditions, the plate test is as sensitive as the fluctuation test, and furthermore has some obvious advantages of speed and economy.
153 MacMillan, S., and Margaret Fox, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX (England) The measurement o f induced mutation frequencies in V79 Chinese hamster cells: a reappraisal o f methodology