- Email: [email protected]
Assessing the foliar diseases (Septoria tritici) tolerance of modern genetics wheat varieties, compared to the classic ones – Fungicide treatments effect
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Abstracts / Journal of Biotechnology 231S (2016) S4–S109
neutrophils, macrophages, and altered regulation of the gene could influence susceptibility to breast cancer. PCR-RFLP was carried out for searching polymorphism LSP1 gene rs 909116 in two main ethnical groups of Kazakhstan. The patient group consisted from 375 Kazakh and 254 Russian with histologically confirmed diagnosis breast cancer and the control group was formed from 286 Russian and 388 Kazakh healthy women. In this study were obtained the following results, statistically significant differences between alleles frequencies (2 = 9.78; p = 0.001) and genotypes (2 = 9.71; p = 0.007) distribution was observed in Kazakh ethnic group. No statistically significant difference was observed for allele frequencies (2 = 0.0002; p = 0.987) and genotypes distribution (2 = 0.005; p = 0.997) in Russian ethnic group. The study results suggest that rs 909116 of LSP1 gene was significantly associated with breast cancer risk in Kazakh ethnic group and can be used as marker to detect BC.
Physiological stress caused by heavy metals in Euglena gracilis and its indicators Suncica Beluhan, Mirela Ivancic Santek ∗ , Bozidar Santek Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia E-mail address: [email protected] (M.I. Santek).
Institute of Agricultural Economics, Romanian Academy, Bucharest, Romania 2 Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timis¸oara, Romania
The effect of different concentrations of Cu2+ , Pb2+ , Co2+ and Hg2+ (0.5–2.0 mM) ions on motility, cell shape, chlorophyll a and b and total carotenoids content, proline accumulation and palmella stage in Euglena gracilis were examined. The toxicity of the heavy metals was dependent on whether cells were cultivated 14 days in the dark (heterotrophy) or under illumination (autotrophy). E. gracilis cells showed improved heavy metal tolerance to Cu2+ and Pb2+ ions (greater cell density and motility), together with better photosynthetic capacity. Photosynthetic pigments and cell shape were found to be more sensitive to Hg2+ and Co2+ ions. Motility and cell shape were strongly impaired immediately upon exposure to the heavy metals, but with the increasing treatment time these parameters showed acclimatization to the stress except to Hg2+ ions. Exposure of E. gracilis to elevated concentration of heavy metals led to accumulation of free proline, as well as palmella stage of E. gracilis life circle. The accumulation of proline was triggered within a few hours of metal exposure, while the cysts formation occurs after few days of treatment. Results suggest that the capacity to tolerate heavy metals by E. gracilis may have differences when cells are grown in the dark or under illumination.
E-mail address: [email protected] (A. Florea).
http://dx.doi.org/10.1016/j.jbiotec.2016.05.334
http://dx.doi.org/10.1016/j.jbiotec.2016.05.332 Agri-food organic products – A fast increasing market Camelia Gavrilescu 1 , Adina Florea 2,∗ , Saida David 2 , Alin Popescu 2 , Nicoleta Mateoc Sîrb 2 1
Organic agriculture increased significantly worldwide in the last decades – organic farmland increased by 300% since 1999. Advantages on the production side are linked with the environmental gains resulting from non-polluting agricultural practices; while on the consumption side are linked to healthier agri-food products and the increasing consumers’ awareness of the benefits of healthy nutrition. Consequently, both supply and demand for organic agrifood products increased significantly. In the world organic retail sales (2014), USA has the largest share (43%), followed by EU-28 (38%) and China (6%), which is the fastest growing market. International trade in organic products showed also a steep upward trend, due to the fact that the largest producing regions (Oceania and Latin America have together more than half of the world organic farmland) are different from the largest consuming regions (USA and EU-28 are accounting together about 4/5 of the world organic retail sales). After EU accession, Romania entered as well in the organic export market. The present study are conducted within research center called “Centre of Excellence for Sustainable Rural Development of Romania” within BUASVM “King Michael I of Romania from Timisoara”, analyzing the latest trends in the organic agri-food markets, in EU-28 and at world level, in terms of demand, consumption and trade, taking into account also the opportunities opened by the US-EU Organic Equivalency Arrangement. http://dx.doi.org/10.1016/j.jbiotec.2016.05.333
Assessing the foliar diseases (Septoria tritici) tolerance of modern genetics wheat varieties, compared to the classic ones – Fungicide treatments effect Cristian Florinel Cioineag 1,∗ , Stelica Cristea 1 , Mihai Berca 2 1 Department of Agriculture; University of Agronomic Science and Veterinary Medicine Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania 2 Department of Management, Economic Engineering in Agriculture and Rural Development, University of Agronomic Science and Veterinary Medicine Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania
E-mail address: [email protected] (C.F. Cioineag). The new biotechnologies for creating wheat varieties by using genetics allow induction into the plants genome of some genes sequences, which increase their resistance toward the dangerous foliar diseases (Fusarium, Septoria, Puccinia and so on). Observations made in the experimental fields from Modelu (Calarasi) and Teleorman (both locations being situated in the south part of Romania), during the 2013/2014 agricultural year, revealed a very significant difference between the control (classic) genetic material and the new variety of premium wheat, Adesso. The difference was analyzed on the varieties tolerance toward the foliar diseases (Septoria tritici) in spring-summer 2014. It has been found that, in untreated variant, Adesso variety registered an attack degree (AD) of 14.26%, in comparison with 64.88% in case of the control variety. To bring
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
the control variety to the level of untreated Adesso variety, 3 treatments were necessary. On the other hand, a single treatment with fungicides applied to the Adesso variety has reduced the attack degree with only 4.7%, a response considered to be insignificant and unjustified from economically and ecologically point of view. Yields level was in inverse proportion to the attack degree evolution, being favourable with +29% to the same Adesso variety. In this way can be assessed that the Environmental Toxicological Impact of (ETI) generated by the fungicide treatments application it was with approximately 25% in behalf of the premium wheat variety in the study (ETIAdesso = 4 × ETIControl ). http://dx.doi.org/10.1016/j.jbiotec.2016.05.335 P (Tannic Acid) hydrogel template for in situ metal nanoparticle preparation and use as catalyst for elimination of toxic organic compounds Coskun Silan 1,∗ , Selin Sagbas 2 , Mehtap Sahiner 3 , Nurettin Sahiner 2 1
School of Medicine, Department Pharmacology, Canakkale Onsekiz Mart University, Turkey 2 Faculty of Science & Arts, Chemistry Department & Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University, Turkey 3 Leather Engineering Departments, Ege University, Turkey E-mail address: [email protected] (C. Silan). Hydrogels based on natural tannic acid (TA) as poly (Tannnic Acid) (p(TA)) were synthesized using trimethylolpropane triglycidyl ether (TMPGDE) as crosslinker. The prepared p(TA) hydrogel was treated with sodium hydroxide (NaOH) and ammonia (NH3 ) to increase the absorption capacity of Co (II), Ni (II), and Cu (II) ions. Then, the metal ion loaded p(TA) hydrogel was treated with NaBH4 to obtain the corresponding metal nanoparticles within p(TA) network. The prepared p(TA)-M (M:Co, Ni, Cu) composites were then used as catalyst in the reduction of toxic organic compounds such as 4-nitrophenol (4-NP), 2-nitrophenol (2-NP), and organic dyes such as methylene blue (MB) and eosin Y (EY). Bare and the modified p(TA) hydrogel were characterized via FT-IR, thermogravimetric analyzer (TGA), zeta potential measurements, and the metal nanoparticles inside p(TA)-M composite were visulized by TEM. The metal ions content of p(TA)-M composite hydrogels were determined by the solution of metal nanoparticles within p(TA) network by HCl treatment, and determining their amouth by Atomic Absorption Spectroscopy (AAS). The effects of metal nanoparticle types on 4-NP and 2-NP reduction and MB and EY decolorization reactions were measured. Also, various parameters such as reaction temperatures, reusability of the catalysts, and so on were investigated. http://dx.doi.org/10.1016/j.jbiotec.2016.05.336
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Search of ESR1 gene polymorphisms association in Kazakhs and Russians with BC risk D.D. Mukushkina ∗ , A.O. Abaildayev, A.Y. Khodayeva, E.E. Ashirbekov, Sh.Zh. Talaeva, Z.B. Esimsiitova, A.K. Khanseitova, N.A. Aitkhozhina M. A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan E-mail address: dina [email protected] (D.D. Mukushkina). Breast cancer (BC) occupies a leading position among oncological diseases in most countries of the world. The development of BC is related to effect of estrogen receptors, encoded by genes, including ESR1, which can be considered as a molecular marker for personalized treatment. In the research, conducted by case-control method, ESR1 gene testing (rs2046210, rs3757318) was carried out by PCR-RFLP. Association of ESR1 gene polymorphisms with BC has been identified neither in Kazakh (rs2046210: p = 0.54 for genotypes, p = 0.32 for alleles; rs3757318: p = 0.4 for genotypes, p = 0.2 for the alleles), nor in Russian ethnic group (rs2046210: p = 0.48 for genotypes, p = 0.42 for alleles; rs3757318: p = 0.82 for genotypes, p = 0.55 for alleles). Data on the distribution of genotypes and allele frequencies corresponded to HWE. Despite of number studies had shown positive association of rs2046210, rs3757318, our obtained results differ from these. For instance, in meta-analysis, containing 11 studies with 62.891 cases and 65.635 controls, the rs3757318 – A allele was significantly associated with BC risk (p < 0.001). Consequently, ESR1 gene polymorphisms (rs2046210, rs3757318) cannot be considered as a breast cancer marker in Kazakh and Russian ethnic groups of Kazakhstan, despite the association with increased BC risk in the other world populations. http://dx.doi.org/10.1016/j.jbiotec.2016.05.337 Influence of accommodation area on welfare fattening cattle Ioana Andronie ∗ , Cristian Mortasivu, Monica Parvu, Viorel Andronie, Violeta Simion Spiru Haret University, Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (I. Andronie). Nowadays, in the intensive livestock farming major importance is assigned to ensure the animals’ comfort. Although the production of beef in Romania has not yet spread wide, the objective of this study was set to investigate how the type of floor can influence the welfare of fattening beef cattle in two commercial farms, assessing their health status, behavior and performances. The study focused on a number of 168 fattening cattle housed on slatted floor (group A, n: 88) and animals accommodated in shelters providing continuous floor with straw litter (group B, n: 80). Lameness incidence ranged from 1.2% in group B and 2.2% in group A and body hygiene registered a higher percentage of animals in group B (5%) than in group A (1.1%). Among the behavioral manifestations, rest was recorded in a higher percentage in group B (39%) than in group A (20%) and the average daily gain was higher in group A (1310 g/day) than group B (1050 g/day). The results of this study showed that in this fattening cattle phase the type of floor influenced not only the beef cattle productivity but also their welfare. http://dx.doi.org/10.1016/j.jbiotec.2016.05.338
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
neutrophils, macrophages, and altered regulation of the gene could influence susceptibility to breast cancer. PCR-RFLP was carried out for searching polymorphism LSP1 gene rs 909116 in two main ethnical groups of Kazakhstan. The patient group consisted from 375 Kazakh and 254 Russian with histologically confirmed diagnosis breast cancer and the control group was formed from 286 Russian and 388 Kazakh healthy women. In this study were obtained the following results, statistically significant differences between alleles frequencies (2 = 9.78; p = 0.001) and genotypes (2 = 9.71; p = 0.007) distribution was observed in Kazakh ethnic group. No statistically significant difference was observed for allele frequencies (2 = 0.0002; p = 0.987) and genotypes distribution (2 = 0.005; p = 0.997) in Russian ethnic group. The study results suggest that rs 909116 of LSP1 gene was significantly associated with breast cancer risk in Kazakh ethnic group and can be used as marker to detect BC.
Physiological stress caused by heavy metals in Euglena gracilis and its indicators Suncica Beluhan, Mirela Ivancic Santek ∗ , Bozidar Santek Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia E-mail address: [email protected] (M.I. Santek).
Institute of Agricultural Economics, Romanian Academy, Bucharest, Romania 2 Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timis¸oara, Romania
The effect of different concentrations of Cu2+ , Pb2+ , Co2+ and Hg2+ (0.5–2.0 mM) ions on motility, cell shape, chlorophyll a and b and total carotenoids content, proline accumulation and palmella stage in Euglena gracilis were examined. The toxicity of the heavy metals was dependent on whether cells were cultivated 14 days in the dark (heterotrophy) or under illumination (autotrophy). E. gracilis cells showed improved heavy metal tolerance to Cu2+ and Pb2+ ions (greater cell density and motility), together with better photosynthetic capacity. Photosynthetic pigments and cell shape were found to be more sensitive to Hg2+ and Co2+ ions. Motility and cell shape were strongly impaired immediately upon exposure to the heavy metals, but with the increasing treatment time these parameters showed acclimatization to the stress except to Hg2+ ions. Exposure of E. gracilis to elevated concentration of heavy metals led to accumulation of free proline, as well as palmella stage of E. gracilis life circle. The accumulation of proline was triggered within a few hours of metal exposure, while the cysts formation occurs after few days of treatment. Results suggest that the capacity to tolerate heavy metals by E. gracilis may have differences when cells are grown in the dark or under illumination.
E-mail address: [email protected] (A. Florea).
http://dx.doi.org/10.1016/j.jbiotec.2016.05.334
http://dx.doi.org/10.1016/j.jbiotec.2016.05.332 Agri-food organic products – A fast increasing market Camelia Gavrilescu 1 , Adina Florea 2,∗ , Saida David 2 , Alin Popescu 2 , Nicoleta Mateoc Sîrb 2 1
Organic agriculture increased significantly worldwide in the last decades – organic farmland increased by 300% since 1999. Advantages on the production side are linked with the environmental gains resulting from non-polluting agricultural practices; while on the consumption side are linked to healthier agri-food products and the increasing consumers’ awareness of the benefits of healthy nutrition. Consequently, both supply and demand for organic agrifood products increased significantly. In the world organic retail sales (2014), USA has the largest share (43%), followed by EU-28 (38%) and China (6%), which is the fastest growing market. International trade in organic products showed also a steep upward trend, due to the fact that the largest producing regions (Oceania and Latin America have together more than half of the world organic farmland) are different from the largest consuming regions (USA and EU-28 are accounting together about 4/5 of the world organic retail sales). After EU accession, Romania entered as well in the organic export market. The present study are conducted within research center called “Centre of Excellence for Sustainable Rural Development of Romania” within BUASVM “King Michael I of Romania from Timisoara”, analyzing the latest trends in the organic agri-food markets, in EU-28 and at world level, in terms of demand, consumption and trade, taking into account also the opportunities opened by the US-EU Organic Equivalency Arrangement. http://dx.doi.org/10.1016/j.jbiotec.2016.05.333
Assessing the foliar diseases (Septoria tritici) tolerance of modern genetics wheat varieties, compared to the classic ones – Fungicide treatments effect Cristian Florinel Cioineag 1,∗ , Stelica Cristea 1 , Mihai Berca 2 1 Department of Agriculture; University of Agronomic Science and Veterinary Medicine Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania 2 Department of Management, Economic Engineering in Agriculture and Rural Development, University of Agronomic Science and Veterinary Medicine Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania
E-mail address: [email protected] (C.F. Cioineag). The new biotechnologies for creating wheat varieties by using genetics allow induction into the plants genome of some genes sequences, which increase their resistance toward the dangerous foliar diseases (Fusarium, Septoria, Puccinia and so on). Observations made in the experimental fields from Modelu (Calarasi) and Teleorman (both locations being situated in the south part of Romania), during the 2013/2014 agricultural year, revealed a very significant difference between the control (classic) genetic material and the new variety of premium wheat, Adesso. The difference was analyzed on the varieties tolerance toward the foliar diseases (Septoria tritici) in spring-summer 2014. It has been found that, in untreated variant, Adesso variety registered an attack degree (AD) of 14.26%, in comparison with 64.88% in case of the control variety. To bring
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
the control variety to the level of untreated Adesso variety, 3 treatments were necessary. On the other hand, a single treatment with fungicides applied to the Adesso variety has reduced the attack degree with only 4.7%, a response considered to be insignificant and unjustified from economically and ecologically point of view. Yields level was in inverse proportion to the attack degree evolution, being favourable with +29% to the same Adesso variety. In this way can be assessed that the Environmental Toxicological Impact of (ETI) generated by the fungicide treatments application it was with approximately 25% in behalf of the premium wheat variety in the study (ETIAdesso = 4 × ETIControl ). http://dx.doi.org/10.1016/j.jbiotec.2016.05.335 P (Tannic Acid) hydrogel template for in situ metal nanoparticle preparation and use as catalyst for elimination of toxic organic compounds Coskun Silan 1,∗ , Selin Sagbas 2 , Mehtap Sahiner 3 , Nurettin Sahiner 2 1
School of Medicine, Department Pharmacology, Canakkale Onsekiz Mart University, Turkey 2 Faculty of Science & Arts, Chemistry Department & Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University, Turkey 3 Leather Engineering Departments, Ege University, Turkey E-mail address: [email protected] (C. Silan). Hydrogels based on natural tannic acid (TA) as poly (Tannnic Acid) (p(TA)) were synthesized using trimethylolpropane triglycidyl ether (TMPGDE) as crosslinker. The prepared p(TA) hydrogel was treated with sodium hydroxide (NaOH) and ammonia (NH3 ) to increase the absorption capacity of Co (II), Ni (II), and Cu (II) ions. Then, the metal ion loaded p(TA) hydrogel was treated with NaBH4 to obtain the corresponding metal nanoparticles within p(TA) network. The prepared p(TA)-M (M:Co, Ni, Cu) composites were then used as catalyst in the reduction of toxic organic compounds such as 4-nitrophenol (4-NP), 2-nitrophenol (2-NP), and organic dyes such as methylene blue (MB) and eosin Y (EY). Bare and the modified p(TA) hydrogel were characterized via FT-IR, thermogravimetric analyzer (TGA), zeta potential measurements, and the metal nanoparticles inside p(TA)-M composite were visulized by TEM. The metal ions content of p(TA)-M composite hydrogels were determined by the solution of metal nanoparticles within p(TA) network by HCl treatment, and determining their amouth by Atomic Absorption Spectroscopy (AAS). The effects of metal nanoparticle types on 4-NP and 2-NP reduction and MB and EY decolorization reactions were measured. Also, various parameters such as reaction temperatures, reusability of the catalysts, and so on were investigated. http://dx.doi.org/10.1016/j.jbiotec.2016.05.336
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Search of ESR1 gene polymorphisms association in Kazakhs and Russians with BC risk D.D. Mukushkina ∗ , A.O. Abaildayev, A.Y. Khodayeva, E.E. Ashirbekov, Sh.Zh. Talaeva, Z.B. Esimsiitova, A.K. Khanseitova, N.A. Aitkhozhina M. A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan E-mail address: dina [email protected] (D.D. Mukushkina). Breast cancer (BC) occupies a leading position among oncological diseases in most countries of the world. The development of BC is related to effect of estrogen receptors, encoded by genes, including ESR1, which can be considered as a molecular marker for personalized treatment. In the research, conducted by case-control method, ESR1 gene testing (rs2046210, rs3757318) was carried out by PCR-RFLP. Association of ESR1 gene polymorphisms with BC has been identified neither in Kazakh (rs2046210: p = 0.54 for genotypes, p = 0.32 for alleles; rs3757318: p = 0.4 for genotypes, p = 0.2 for the alleles), nor in Russian ethnic group (rs2046210: p = 0.48 for genotypes, p = 0.42 for alleles; rs3757318: p = 0.82 for genotypes, p = 0.55 for alleles). Data on the distribution of genotypes and allele frequencies corresponded to HWE. Despite of number studies had shown positive association of rs2046210, rs3757318, our obtained results differ from these. For instance, in meta-analysis, containing 11 studies with 62.891 cases and 65.635 controls, the rs3757318 – A allele was significantly associated with BC risk (p < 0.001). Consequently, ESR1 gene polymorphisms (rs2046210, rs3757318) cannot be considered as a breast cancer marker in Kazakh and Russian ethnic groups of Kazakhstan, despite the association with increased BC risk in the other world populations. http://dx.doi.org/10.1016/j.jbiotec.2016.05.337 Influence of accommodation area on welfare fattening cattle Ioana Andronie ∗ , Cristian Mortasivu, Monica Parvu, Viorel Andronie, Violeta Simion Spiru Haret University, Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (I. Andronie). Nowadays, in the intensive livestock farming major importance is assigned to ensure the animals’ comfort. Although the production of beef in Romania has not yet spread wide, the objective of this study was set to investigate how the type of floor can influence the welfare of fattening beef cattle in two commercial farms, assessing their health status, behavior and performances. The study focused on a number of 168 fattening cattle housed on slatted floor (group A, n: 88) and animals accommodated in shelters providing continuous floor with straw litter (group B, n: 80). Lameness incidence ranged from 1.2% in group B and 2.2% in group A and body hygiene registered a higher percentage of animals in group B (5%) than in group A (1.1%). Among the behavioral manifestations, rest was recorded in a higher percentage in group B (39%) than in group A (20%) and the average daily gain was higher in group A (1310 g/day) than group B (1050 g/day). The results of this study showed that in this fattening cattle phase the type of floor influenced not only the beef cattle productivity but also their welfare. http://dx.doi.org/10.1016/j.jbiotec.2016.05.338