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T P2X7 receptor gene polymorphism with susceptibility to tuberculosis in Zahedan, southeast Iran
S306
Abstracts
Poster – [A-10-1148-1] Association of − 762C/T P2X7 receptor gene polymorphism with susceptibility to tuberculosis in Zahedan, southeast Iran Gholamreza Bahari, Mohsen Taheri, Abdolkarim Moazeni-Roodi, Mohammad Naderi, Mohammad Hashemi Zahedan, Iran E-mail addresses: [email protected] (G. Bahari), [email protected] (M. Taheri), [email protected] (A. Moazeni-Roodi), [email protected] (M. Naderi), [email protected] (M. Hashemi) Introduction: Tuberculosis, caused by Mycobacterium tuberculosis, is a public health problem and remains a major cause of morbidity and mortality around the world. One third of the earth's population is infected with TB but only 10% individuals infected will develop the disease.This fact indicate that genetic factors play an important role in susceptibility to TB. The purinergic P2X7 receptors shown to induce killing of intracellular M. tuberculosis, followed by apoptosis of the infected macrophage. Methods: This case–control study was done on 151 PTB patients (58 males, 93 females; age; 48.77 ± 21.33) and 151 healthy subjects (50 males, 101 females; age; 45.23 ± 16.04). Genotype analysis of −762C/T P2X7 receptor gene polymorphism was done using amplification refractory mutation system-PCR (ARMS-PCR). The frequency of −762 CC, CT, and TT were 47.7% (72/151), 35.8%(54/151) and 16.5%(25/151) in patients, While the frequency in healthy subjects were 68.2%(103/151), 27.8%(42/151), 4%(6/151) respectively. Results: We found that CT genotype is a risk factor for TB (OR= 1.893, 95%CI = 1.112–3.043, p =0.021) as well as TT genotype (OR= 5.961, 95%CI = 2.327–15.27, p < 0.0001). Frequency of T allele in PTB was 34.4%(104/302), and in healthy subjects was 17.9%(54/302). T allele is a risk factor for TB (OR = 2.412, 95%CI = 1.653–3.521, p <0.0001). Conclusion: We conclude that − 762C/T P2X7 receptor gene polymorphism is associated with susceptibility to tuberculosis and may be a new risk factor for the development of tuberculosis ina sample Iranian population. Keywords: Tuberculosis, Polymorphism, P2X7 receptor doi:10.1016/j.clinbiochem.2011.08.773
Oral – [A-10-1149-1] HIF-1alpha overexpression improves mesenchymal stem cells angiogenesis potential Razban Vahida, S. Lotfi Abbasa, Soleimani Masudb, Hossein Ahmadia, Mohammad Massumia, Sahar Khajehb a Pajuhesh town, Tehran-Karaj highway, Tehran, Iran b AleAhmad-Chamran highway cross, Tehran, Iran E-mail addresses: [email protected] (R. Vahid), [email protected] (S. Khajeh) Introduction: Therapeutic angiogenesis is a promising approach to treat patients with cardiovascular disease, and will likely be critical to engineering large tissues. HIF-1alpha is a key determinant of oxygen-dependent gene regulation in angiogenesis and may be beneficial in cell therapy of ischemic heart disease. In this study, the tube formation and angiogenesis potential of HIF-1alpha overexpression in Bone Marrow Mesenchymal Stem Cells (BMMSCs) was studied. Methods: HIF-1alpha cDNA was cloned into a transfer lenti viral vector (iG2) which is expressed along with eGFP under the control of strong SFFV promoter. To produce viral particles, iG2, mD2 and PAX
plasmids were co-transfected in to HEK-293.T cells by calciumphosphate protocol. Viruses were harvested and used for transduction of BMSCs. Tube formation was evaluated by seeding infected cells and non-infected cells as control on matrigel coated plates. ELISA was used to measure the VEGF secretion in the culture medium. Results: Around 90% of HEK-293.T cells were transfected and GFP emission showed that infection of BMSCs was successfully performed over 85%. Enhanced VEGF secretion was observed in HIF-1alpha over expressed-BMSCs compared to non infected cells. Interestingly, tube network formation was quite superior in HIF-1alpha overexpressedBMSCs. Conclusion: VEGF secretion is mainly regulate by HIF-1alpha and is the predominant factor involved in angiogenesis. Numerous angiogenic factors are regulated by HIF-1alpha and elevated secretion of VEGF by overexpression of HIF-1alpha may consider as an important reason in promoting network formation. Significant improved tube formation of HIF-1alpha over expressed-BMSCs indicates the involvement of HIF-1alpha overexpression in angiogenesis. Keywords: Angiogenesis, HIF-1alpha, VEGF, Mesenchymal stem cell doi:10.1016/j.clinbiochem.2011.08.774
Poster – [A-10-1182-1] Maternal Serum Interlukine-2 and Interlukine-10 in Preeclampsia and Normal Pregnancy Ayati Sedigheh, Modarres ardabili Hossein, Modarres ardabili Zahrah, Sheikh andalibi Mohammad Sobhan, Akhavan Rezayat Amir Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (A. Sedigheh) Introduction: Preeclampsia is a pregnancy-specific complex disease with increased maternal and fetal morbidity and mortality. Changes of serum interlukine-2 and interlukine-10 levels have been suggested as one of the pathophysiologic mechanisms in preeclampsia. The aim of this study is to compare the concentration of interlukine-2 and interlukine-10 in preeclampsia and normal pregnancy. Methods: This study was performed on 17 mild preeclampsia cases, 20 severe preeclampsia and 41 normal pregnant women. We took 5 cm3 blood from each woman and maternal serum interlukine-2 and interlukine-10 concentration was measured for each group. Results: There was no significant difference between mild preeclampsia cases, severe preeclampsia and normal pregnancy group as they were compared in the view of maternal serum interlukine-2 and interlukine-10 concentration. Conclusion: In the present study, sera levels of inflammatory and anti-inflammatory cytokines were not different between patients with preeclampsia and normal controls. Therefore, changes in interlukine-2 and interlukine-10 levels can not be the important etiology of preeclampsia. Keywords: Preeclampsia, Normal pregnancy, Interlukine-2, Interlukine-10 doi:10.1016/j.clinbiochem.2011.08.775
Oral – [A-10-1192-1] Evaluation of in vitro gene expression of GDNF in transfected bone marrow stromal cells Ali Nourizadeha, Tiraihi Takia, Seyed A. Mesbah-Namina, Masoumeh Rajabib
Abstracts
Poster – [A-10-1148-1] Association of − 762C/T P2X7 receptor gene polymorphism with susceptibility to tuberculosis in Zahedan, southeast Iran Gholamreza Bahari, Mohsen Taheri, Abdolkarim Moazeni-Roodi, Mohammad Naderi, Mohammad Hashemi Zahedan, Iran E-mail addresses: [email protected] (G. Bahari), [email protected] (M. Taheri), [email protected] (A. Moazeni-Roodi), [email protected] (M. Naderi), [email protected] (M. Hashemi) Introduction: Tuberculosis, caused by Mycobacterium tuberculosis, is a public health problem and remains a major cause of morbidity and mortality around the world. One third of the earth's population is infected with TB but only 10% individuals infected will develop the disease.This fact indicate that genetic factors play an important role in susceptibility to TB. The purinergic P2X7 receptors shown to induce killing of intracellular M. tuberculosis, followed by apoptosis of the infected macrophage. Methods: This case–control study was done on 151 PTB patients (58 males, 93 females; age; 48.77 ± 21.33) and 151 healthy subjects (50 males, 101 females; age; 45.23 ± 16.04). Genotype analysis of −762C/T P2X7 receptor gene polymorphism was done using amplification refractory mutation system-PCR (ARMS-PCR). The frequency of −762 CC, CT, and TT were 47.7% (72/151), 35.8%(54/151) and 16.5%(25/151) in patients, While the frequency in healthy subjects were 68.2%(103/151), 27.8%(42/151), 4%(6/151) respectively. Results: We found that CT genotype is a risk factor for TB (OR= 1.893, 95%CI = 1.112–3.043, p =0.021) as well as TT genotype (OR= 5.961, 95%CI = 2.327–15.27, p < 0.0001). Frequency of T allele in PTB was 34.4%(104/302), and in healthy subjects was 17.9%(54/302). T allele is a risk factor for TB (OR = 2.412, 95%CI = 1.653–3.521, p <0.0001). Conclusion: We conclude that − 762C/T P2X7 receptor gene polymorphism is associated with susceptibility to tuberculosis and may be a new risk factor for the development of tuberculosis ina sample Iranian population. Keywords: Tuberculosis, Polymorphism, P2X7 receptor doi:10.1016/j.clinbiochem.2011.08.773
Oral – [A-10-1149-1] HIF-1alpha overexpression improves mesenchymal stem cells angiogenesis potential Razban Vahida, S. Lotfi Abbasa, Soleimani Masudb, Hossein Ahmadia, Mohammad Massumia, Sahar Khajehb a Pajuhesh town, Tehran-Karaj highway, Tehran, Iran b AleAhmad-Chamran highway cross, Tehran, Iran E-mail addresses: [email protected] (R. Vahid), [email protected] (S. Khajeh) Introduction: Therapeutic angiogenesis is a promising approach to treat patients with cardiovascular disease, and will likely be critical to engineering large tissues. HIF-1alpha is a key determinant of oxygen-dependent gene regulation in angiogenesis and may be beneficial in cell therapy of ischemic heart disease. In this study, the tube formation and angiogenesis potential of HIF-1alpha overexpression in Bone Marrow Mesenchymal Stem Cells (BMMSCs) was studied. Methods: HIF-1alpha cDNA was cloned into a transfer lenti viral vector (iG2) which is expressed along with eGFP under the control of strong SFFV promoter. To produce viral particles, iG2, mD2 and PAX
plasmids were co-transfected in to HEK-293.T cells by calciumphosphate protocol. Viruses were harvested and used for transduction of BMSCs. Tube formation was evaluated by seeding infected cells and non-infected cells as control on matrigel coated plates. ELISA was used to measure the VEGF secretion in the culture medium. Results: Around 90% of HEK-293.T cells were transfected and GFP emission showed that infection of BMSCs was successfully performed over 85%. Enhanced VEGF secretion was observed in HIF-1alpha over expressed-BMSCs compared to non infected cells. Interestingly, tube network formation was quite superior in HIF-1alpha overexpressedBMSCs. Conclusion: VEGF secretion is mainly regulate by HIF-1alpha and is the predominant factor involved in angiogenesis. Numerous angiogenic factors are regulated by HIF-1alpha and elevated secretion of VEGF by overexpression of HIF-1alpha may consider as an important reason in promoting network formation. Significant improved tube formation of HIF-1alpha over expressed-BMSCs indicates the involvement of HIF-1alpha overexpression in angiogenesis. Keywords: Angiogenesis, HIF-1alpha, VEGF, Mesenchymal stem cell doi:10.1016/j.clinbiochem.2011.08.774
Poster – [A-10-1182-1] Maternal Serum Interlukine-2 and Interlukine-10 in Preeclampsia and Normal Pregnancy Ayati Sedigheh, Modarres ardabili Hossein, Modarres ardabili Zahrah, Sheikh andalibi Mohammad Sobhan, Akhavan Rezayat Amir Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (A. Sedigheh) Introduction: Preeclampsia is a pregnancy-specific complex disease with increased maternal and fetal morbidity and mortality. Changes of serum interlukine-2 and interlukine-10 levels have been suggested as one of the pathophysiologic mechanisms in preeclampsia. The aim of this study is to compare the concentration of interlukine-2 and interlukine-10 in preeclampsia and normal pregnancy. Methods: This study was performed on 17 mild preeclampsia cases, 20 severe preeclampsia and 41 normal pregnant women. We took 5 cm3 blood from each woman and maternal serum interlukine-2 and interlukine-10 concentration was measured for each group. Results: There was no significant difference between mild preeclampsia cases, severe preeclampsia and normal pregnancy group as they were compared in the view of maternal serum interlukine-2 and interlukine-10 concentration. Conclusion: In the present study, sera levels of inflammatory and anti-inflammatory cytokines were not different between patients with preeclampsia and normal controls. Therefore, changes in interlukine-2 and interlukine-10 levels can not be the important etiology of preeclampsia. Keywords: Preeclampsia, Normal pregnancy, Interlukine-2, Interlukine-10 doi:10.1016/j.clinbiochem.2011.08.775
Oral – [A-10-1192-1] Evaluation of in vitro gene expression of GDNF in transfected bone marrow stromal cells Ali Nourizadeha, Tiraihi Takia, Seyed A. Mesbah-Namina, Masoumeh Rajabib