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Association study of SLC30A8 gene polymorphisms with the gestational diabetes mellitus in the Iranian women
S132
Abstracts
Poster – [A-10-152-1] Association study of SLC30A8 gene polymorphisms with the gestational diabetes mellitus in the Iranian women Samaneh Enayatia, Arash Hosseinnezhadb, Firouzeh Birami Jamalc, Zhila Maghbulib, Mahtab Khosrofarb a Department of Biology, Faculty of Science, Science and Research Unit, Azad University, Tehran, Iran b Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran c National Institute of Genetic Engineering & Biotechnology, Tehran, Iran E-mail address: [email protected] (S. Enayati) Introduction: Gestational diabetes mellitus (GDM) and type 2 diabetes have a common pathophysiological background, including beta cell disfunction and insulin resistance. In addition, women with GDM have considerably elevated risk of developing type 2 diabetes later in life. Therefore, GDM and type 2 diabetes may have same genetic background. Variation in zinc transporter, member 8 (SLC30A8) gene has been shown to be associated with type 2 diabetes and diabetes-related quantitative traits. Methods: We evaluated the effect of SLC30A8 genetic variation on gestational diabetes mellitus in Iranian women through a case– control study. Study participants were included 100 GDM case and 100 pregnant non-diabetic controls who were evaluated through medical histories, physical examinations, routine clinical laboratory tests, the rate of insulin resistance and insulin response. SLC30A8 R32W genotypes were determined by polymerase chain reaction– restriction fragment length polymorphism (RFLP–PCR). Conclusions: SLC30A8 R32W polymorphism were associated with Gestational Diabetes Mellitus in Iranian patients. Keywords: Gestational diabetes mellitus (GDM), Insulin resistance, SLC30A8, Single Nucleotide Polymorphism (SNP) doi:10.1016/j.clinbiochem.2011.08.313
Poster – [A-10-153-1] “Leucocytaria inversion” in the use of benzodiazepin derivatives Claudio Malema, Roberto Vitalib a Ingeniero Lange 448-General Alvear-province of Mendoza, Argentina b Chapeaurouge 671-General Alvear-province of Mendoza, Argentina E-mail addresses: [email protected] (C. Malem), [email protected] (R. Vitali) Introduction: Since January 2010 and during the following 12 months, 42 female patients, all of them belonging to middle/upper strata of society were studied. They were all undergoing treatment with benzodiazepine derivatives. At the start of this case study they presented marked macrocitosis, very low white blood cells count, evident thrombocitopenia, altered hepatic profile, anemia in the 96% of the cases and coagulation tests severely altered towards hypercoagubility. All of them presented inversion in the leucocytaria formula. Materials and methods: At the start of this study, it was decided to suspend the benzodiazepin derivatives, they were helped with ferrous fumarate so as to raise the hemoglobin level, whose value was between 9 and 9.5 g/dl, and adding prednison as a way to avoid hepatic disorders and also a minimum dose of acenocumarol (adjusted to INR) to prevent prothrombotic effects. At the next blood control, all the hematological values gradually varied positively, consequently, it was decided to suspend the ferrous fumarate and acenocoumarol, but to keep the prednison until the complete hepatic recovery. Results: After 60 days, when the corresponding blood control was done, it was shown that the patients had got normal analytical values, with the hemoglobin dosage between 12 and 14.5 g/dl,
accompanied with normocitic elements and values of count of white blood cells in normal parameters, thrombocytes between 185 and 395 × 10/ml and hepatic dosages with reference values according to normal individuals. Conclusion: The search of psycho-social stability has brought about massive consumption on a large scale of benzodiazepin derivatives. The abuse in the use of these derivatives causes significant quali-quantitative alterations in the hematological profile. When these derivatives are suspended, the patients recover the analytical hematological values of normal individuals. Keywords: leucocytaria inversion, Benzodiazepin, Macrocitocis doi:10.1016/j.clinbiochem.2011.08.314
Poster – [A-10-153-2] Following the case of pregnant women with deficiency of von Willebrand factor during pregnancy and purpureum Claudio Malem Ingeniero Lange 448, General Alvear, province of Mendoza, Argentina E-mail address: [email protected] Fourteen pregnant women with a diagnosis of von Willebrand disease were studied. They all belonged to an indigent group in extreme need. The monitoring was done at three month's time, at six month's time and from then on every month until delivery and during six weeks after childbirth. The tests included red blood cells count, blood platelets count and Quick time. After the three month period of pregnancy, when the patient attended the routine gyneco-obstetrical consultation, she was required the corresponding analytical tests, and here is when the future problems of these patients at high social risk start to be clear-sighted. The laboratory analytical values were corrected by using desmopresin acetate helped with ferrous anhidro succinate, so there was no need of blood transfusion to keep the blood volume. Reaching the third period of pregnancy with laboratory tests corrected, delivery was achieved successfully without physiologic nor psychological risks neither for fetus nor mothers, having them both good health recovery without any problems. Despite the social and psychological surrounding conditions in which this event took place and although the patients were poverty-striken, childbirth was reached without any physiologic risks neither for mothers nor babies. They all had free treatment, the corresponding medicines, gynecological care and analytical laboratory tests. Everything was free for the patients and was supplied by the author's assistance. Keywords: von Willebrand, Indigent, Pregnancy doi:10.1016/j.clinbiochem.2011.08.315
Poster – [A-10-158-1] Toxicity effects of hydroxy coumarin drivations on induction of apoptosis in N2A cell line Javad Sargolzaeia, Hamid Sadeghianb, Mohammad Soukhtanlooc, Sanaz Golahmadia a Department of Biology, School of Basic Sciences, Payam Nour University, Mashhad, Iran b Department of Laboratory Science, School of Paramedical science, Mashhad University of Medical Sciences, Mashhad, Iran c Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran E-mail addresses: [email protected] (J. Sargolzaei), [email protected] (H. Sadeghian), [email protected] (M. Soukhtanloo), [email protected] (S. Golahmadi)
Abstracts
Poster – [A-10-152-1] Association study of SLC30A8 gene polymorphisms with the gestational diabetes mellitus in the Iranian women Samaneh Enayatia, Arash Hosseinnezhadb, Firouzeh Birami Jamalc, Zhila Maghbulib, Mahtab Khosrofarb a Department of Biology, Faculty of Science, Science and Research Unit, Azad University, Tehran, Iran b Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran c National Institute of Genetic Engineering & Biotechnology, Tehran, Iran E-mail address: [email protected] (S. Enayati) Introduction: Gestational diabetes mellitus (GDM) and type 2 diabetes have a common pathophysiological background, including beta cell disfunction and insulin resistance. In addition, women with GDM have considerably elevated risk of developing type 2 diabetes later in life. Therefore, GDM and type 2 diabetes may have same genetic background. Variation in zinc transporter, member 8 (SLC30A8) gene has been shown to be associated with type 2 diabetes and diabetes-related quantitative traits. Methods: We evaluated the effect of SLC30A8 genetic variation on gestational diabetes mellitus in Iranian women through a case– control study. Study participants were included 100 GDM case and 100 pregnant non-diabetic controls who were evaluated through medical histories, physical examinations, routine clinical laboratory tests, the rate of insulin resistance and insulin response. SLC30A8 R32W genotypes were determined by polymerase chain reaction– restriction fragment length polymorphism (RFLP–PCR). Conclusions: SLC30A8 R32W polymorphism were associated with Gestational Diabetes Mellitus in Iranian patients. Keywords: Gestational diabetes mellitus (GDM), Insulin resistance, SLC30A8, Single Nucleotide Polymorphism (SNP) doi:10.1016/j.clinbiochem.2011.08.313
Poster – [A-10-153-1] “Leucocytaria inversion” in the use of benzodiazepin derivatives Claudio Malema, Roberto Vitalib a Ingeniero Lange 448-General Alvear-province of Mendoza, Argentina b Chapeaurouge 671-General Alvear-province of Mendoza, Argentina E-mail addresses: [email protected] (C. Malem), [email protected] (R. Vitali) Introduction: Since January 2010 and during the following 12 months, 42 female patients, all of them belonging to middle/upper strata of society were studied. They were all undergoing treatment with benzodiazepine derivatives. At the start of this case study they presented marked macrocitosis, very low white blood cells count, evident thrombocitopenia, altered hepatic profile, anemia in the 96% of the cases and coagulation tests severely altered towards hypercoagubility. All of them presented inversion in the leucocytaria formula. Materials and methods: At the start of this study, it was decided to suspend the benzodiazepin derivatives, they were helped with ferrous fumarate so as to raise the hemoglobin level, whose value was between 9 and 9.5 g/dl, and adding prednison as a way to avoid hepatic disorders and also a minimum dose of acenocumarol (adjusted to INR) to prevent prothrombotic effects. At the next blood control, all the hematological values gradually varied positively, consequently, it was decided to suspend the ferrous fumarate and acenocoumarol, but to keep the prednison until the complete hepatic recovery. Results: After 60 days, when the corresponding blood control was done, it was shown that the patients had got normal analytical values, with the hemoglobin dosage between 12 and 14.5 g/dl,
accompanied with normocitic elements and values of count of white blood cells in normal parameters, thrombocytes between 185 and 395 × 10/ml and hepatic dosages with reference values according to normal individuals. Conclusion: The search of psycho-social stability has brought about massive consumption on a large scale of benzodiazepin derivatives. The abuse in the use of these derivatives causes significant quali-quantitative alterations in the hematological profile. When these derivatives are suspended, the patients recover the analytical hematological values of normal individuals. Keywords: leucocytaria inversion, Benzodiazepin, Macrocitocis doi:10.1016/j.clinbiochem.2011.08.314
Poster – [A-10-153-2] Following the case of pregnant women with deficiency of von Willebrand factor during pregnancy and purpureum Claudio Malem Ingeniero Lange 448, General Alvear, province of Mendoza, Argentina E-mail address: [email protected] Fourteen pregnant women with a diagnosis of von Willebrand disease were studied. They all belonged to an indigent group in extreme need. The monitoring was done at three month's time, at six month's time and from then on every month until delivery and during six weeks after childbirth. The tests included red blood cells count, blood platelets count and Quick time. After the three month period of pregnancy, when the patient attended the routine gyneco-obstetrical consultation, she was required the corresponding analytical tests, and here is when the future problems of these patients at high social risk start to be clear-sighted. The laboratory analytical values were corrected by using desmopresin acetate helped with ferrous anhidro succinate, so there was no need of blood transfusion to keep the blood volume. Reaching the third period of pregnancy with laboratory tests corrected, delivery was achieved successfully without physiologic nor psychological risks neither for fetus nor mothers, having them both good health recovery without any problems. Despite the social and psychological surrounding conditions in which this event took place and although the patients were poverty-striken, childbirth was reached without any physiologic risks neither for mothers nor babies. They all had free treatment, the corresponding medicines, gynecological care and analytical laboratory tests. Everything was free for the patients and was supplied by the author's assistance. Keywords: von Willebrand, Indigent, Pregnancy doi:10.1016/j.clinbiochem.2011.08.315
Poster – [A-10-158-1] Toxicity effects of hydroxy coumarin drivations on induction of apoptosis in N2A cell line Javad Sargolzaeia, Hamid Sadeghianb, Mohammad Soukhtanlooc, Sanaz Golahmadia a Department of Biology, School of Basic Sciences, Payam Nour University, Mashhad, Iran b Department of Laboratory Science, School of Paramedical science, Mashhad University of Medical Sciences, Mashhad, Iran c Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran E-mail addresses: [email protected] (J. Sargolzaei), [email protected] (H. Sadeghian), [email protected] (M. Soukhtanloo), [email protected] (S. Golahmadi)