Axonal regeneration in the central nervous system is enhanced by ependymal cell transplants

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TRANSPLANTATION

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TRANSIENT

YASUO KURIMOTO’.

OF NEURAL STEM CELLS INTO THE EYES OF THE RETINA INJURED

BY

ISCHEMlA.

HIROTO SHIBUKI’,

TORU KUROKAWA’,

NAGAHISA

YOSHIMURA’,

MASAYO

TAKAHASHI’ ‘Dept. of Ophthalmology,

Shinshu University

Visual Science, Kyoto University

School of Medicine, Matsumoto,

Nagano. Japan, %ept of Ophthalmology

and

Graduate School of Medicine, Kyoto, Japan

Stem cells of the central nervous system are capable of differentiating

along multiple central nervous system cell-type lineages.

and their use as graft material is promised to provide new strategies for treatment of neurndegenerative is known that the stem cells are not integrated into the intact retina when they are transplanted

diseases and trauma. It

into the eyes of adult rat. We

examined whether the stem cells can be integrated into the retina injured by transient ischemia. The R-gal labeled stem cells derived from hippocampus ischemia. Transplanted

of adult rat were injected into the vitreous cavity of the eyes whose retina was injured by transient

cells were found to invade into the retinal ganglion cell layer 1 week after the transplantation,

were found to be integrated into the retinal inner nuclear layer and show the appropriate morphology weeks after the transplantation. transplantation

TRANSPLANTATION IMMUNOLOGICAL

TAKESHI KONDOH, Department

HIDEYUKI

of Neurosurgery,

The development transplanted

of immunological

NORIHIKO

system is incomplete

was transfected

1O6cells were transplanted

in the surrounding

the grafted cells

into the host brain. There

cells, as well as astrocytes,

were

tolerance.

NERVOUS

SYSTEM

CELL TRANSPLANTS

CHAKRABORTTY,

and Neurobiology,

for histological

that the xenograts are able to survive in neonatal brain and

IN THE CENTRAL

BY EPENDYMAL

SHUSHOVAN

extending

host brain. Host derived endothelial

REGENERATION

IS ENHANCED

of Anatomy

the brains were processed

is likely playing the main role for immunological

AXONAL

method and subclone

of GFP. In early stage posttransplantation,

in the grafts. The present study demonstrated

Department

by the electroporation

In the late stage, the grafted cell formed long processes

of blood-brain-barrier

KITADA.

rejection of

into newborn S-D rat striatum (n=45).

drug was not given. One to 6 weeks posttransplantation,

MASAAKI

Kobe 650-0017

in neonatal rodent. We studied immunological

The marker gene, pEGFPN-1,

noted marked reactive astrocytes

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Chuou-ku,

in neonatal brain. A human astrocyte cell was obtained from a white matter with gliosis in a

procedure.

tended to form an aggregation.

formation

RAT BRAIN AND

TAMAKI

School of Medicine,

study. All of the grafts were survived with distinct expression

well integrated

INTO NEONATAL

TOLERANCE AKIYAMA,

was selected by G4 18. Approximately Immunosuppressive

retina.

Of HUMAN ASTROCYTES

Kobe University

human astrocytes

routine neurosurgical

of retinal neuronal cells 3

It was suggested that neural stem cells may possibly be used as the donor of neural

to reconstruct the neural network of ischemic-injured

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and they

Postgraduate

AKIRA MIZOGUCHI.

CHIZUKA

IDE

School of Medicine. Kyoto University,

Sakyo-ku, Kyoto. 6068501 Previous studies have shown that avonal regeneration nervous

system.

In this study,

we examined

hardly occured at the area of Wallerian degeneration

the availability

regeneration. The spinal cords of adult rats were partially with fluorescence dye was inserted. In confocal laser microscopy, electronmicroscopy,

between the membranes.

ependymal

in the central

cells of axonal

severed and then choroid plexus of the fourth ventricle

axons were seen to contact cells specifically

These results indicate that ependymal

site in the central nervous system.

cells as the supportive

At 3 days. I week and 3 weeks after operation,

the regenerating

the transplanted

of ependymal

enclosed

rats were perfused

with the transplanted the regenerating

cell transplants

support

labelled

and cryosectioned. ependymal

cells. In

axons with close apposion

axonal regeneration

at the lesion