- Email: [email protected]
Bacterial exopolysaccharides production using food iindustry wastes
Abstracts / Journal of Biotechnology 185S (2014) S18–S36
S35
raw materials provides mash with final alcohol content about 8% (by volume).
found interesting clinical uses, especially in the cancer treatment through a combined action of chemotherapy and gene delivery.
http://dx.doi.org/10.1016/j.jbiotec.2014.07.116
http://dx.doi.org/10.1016/j.jbiotec.2014.07.118
Bacterial exopolysaccharides production using food industry wastes
Physical, mechanical and biological properties of the reinforced salt-leaching porous silk scaffolds
Elena V. Liiaskina ∗ , Victor V. Revin, Mariya I. Nazarkina N.P. Ogarev Mordovia State University, Saransk, Russia E-mail address: [email protected] (E.V. Liiaskina). A current state of fundamental and applied aspects of bacterial exopolysaccharides (EPS) biotechnology was analyzed. EPS are the biotechnological products of high interest due to their unique properties. Bacterial cellulose and xanthan have many applications, particularly in food, pharmaceutical and biomedical fields. At present bacterial cellulose is considered as a perspective material for obtaining of a wide range of nanostructured biomaterials and biocomposites. The goal of our work was to study bacterial cellulose and xanthan production by novel efficient strains using food industry wastes. Gluconacetobacter sucrofermentans and Xanthomonas campestris bacteria cultivation conditions were optimized. The balanced medium provides high yield of bacterial cellulose (7 g/l) and xanthan (25 g/l) and contributes to industrial wastes utilization. The physicochemical properties of bacterial cellulose and xanthan were investigated. http://dx.doi.org/10.1016/j.jbiotec.2014.07.117 Stem Cells, Biomaterials, Tissue Engineering Plasmid DNA microgels for cancer treatment through combination of chemotherapy and gene delivery Diana Barata Costa 1,∗ , Artur Monteiro Valente 2 , João Sampaio Queiroz 1
Piyanun Wangkulangkul 1,∗ , Jirayut Jaipaew 1 , Jirut Meesane 1 , Supaporn Krivimol 2 , Puttisak Puttawibul 1 1
Institute of Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hatyai, Songkla, Thailand 2 Queen Sirikit Sericulture Center, Narathiwat, Thailand E-mail address: neoz [email protected] (P. Wangkulangkul). Porous silk scaffolds prepared by the salt-leaching technique are often used in soft tissue engineering. However, this scaffold type is stiffer with less surface area than porous silk scaffolds prepared by other methods. In this study, we produced reinforced salt-leaching porous scaffolds by soaking the salt-leached scaffolds in 0.125%, 0.25%, 0.5% and 1% (w/v) of fibroin solutions and then they were lyophilized. The scanning electron micrograph of 0.5% and 1.0% reinforced scaffolds showed a net-like structure in each scaffold pore. The 0.25% reinforced scaffolds demonstrated interconnecting bridges in the scaffold pores and the 0.125% reinforced scaffold illustrated a thicker scaffold wall than the native salt-leaching scaffold. Moreover, the reinforced group was significantly more stable than the native scaffolds. Furthermore, the compressive moduli of 0.125%, 0.25% and 0.5% reinforcements were less than the native. However, the compressive modulus of the 1.0% reinforcement was not significantly different from the native. For biomedical applications, we investigated the feasibility of adipose tissue engineering on a reinforced scaffold by culturing the pre-adipocyte 3T3-L1 cell line and evaluated their proliferation in the scaffolds. The results showed that all scaffolds were not toxic and the proliferation of the 3T3-L1 cell in all scaffolds was not significantly different.
1
CICS-UBI – Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal 2 Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal E-mail address: [email protected] (D.B. Costa).
Plasmid DNA (pDNA) was efficiently encapsulated into biocompatible microgels by an inverse microemulsion polymerization method using ethylene glycol diglycidyl ether (EGDE) as cross-linker. Plasmid DNA and an anticancer drug, doxorubicin (DOX), could both be loaded with high encapsulation efficiency and released from pDNA microgels; quite relevant is also the less toxic effect of the incorporated drug, as compared with naked DOX. Photodisruption of microgels can be used as a strategy to enhance release. Mathematical models were applied to fully characterize the pDNA and drug release profiles. The delivery of the encapsulated pDNA shows the capability of cell internalization and transfection in vitro resulting in the expression of the p53 protein. The effect of this system on cell viability inhibition was evaluated in cancer Hela cells. The treatment with a pDNA/doxorubicin loaded microgel improves cell apoptosis. Compared with pDNA microgel or free drug, the co-delivery system has a stronger potential to suppress the development of cancer cells. This pDNA photodegradable system can
http://dx.doi.org/10.1016/j.jbiotec.2014.07.119 Natural-based polymer scaffolds with microwave induced porosity Antonella Giuri 1 , Vincenzo Maria De Benedictis 2 , Maria Grazia Raucci 3 , Christian Demitri 1,∗ , Alessndro Sannino 1 1 University of Salento, Department of Innovation for Engineering, Via Monteroni, Campus Ecotekne, 73100 Lecce, Italy 2 High Tech District – DHITECH Scarl, via S. Trinchese, 61, 73100 Lecce, Italy 3 Institute of Polymers, Composites and Biomaterials, National Research Council of Italy (IPCB-CNR), Italy
E-mail address: [email protected] (C. Demitri). In this work, interconnected porous material was obtained by a new foaming method based on the combination of two steps. Initially, a stable physical foaming was induced using a surfactant (i.e. pluronic) as blowing agent of a homogeneous blend of polysaccharide and polyethylene glycol diacrylate solution. Furthermore, the porous structure of the foam was chemically stabilized by radical polymerization induced by homogeneous heating of the sample
S35
raw materials provides mash with final alcohol content about 8% (by volume).
found interesting clinical uses, especially in the cancer treatment through a combined action of chemotherapy and gene delivery.
http://dx.doi.org/10.1016/j.jbiotec.2014.07.116
http://dx.doi.org/10.1016/j.jbiotec.2014.07.118
Bacterial exopolysaccharides production using food industry wastes
Physical, mechanical and biological properties of the reinforced salt-leaching porous silk scaffolds
Elena V. Liiaskina ∗ , Victor V. Revin, Mariya I. Nazarkina N.P. Ogarev Mordovia State University, Saransk, Russia E-mail address: [email protected] (E.V. Liiaskina). A current state of fundamental and applied aspects of bacterial exopolysaccharides (EPS) biotechnology was analyzed. EPS are the biotechnological products of high interest due to their unique properties. Bacterial cellulose and xanthan have many applications, particularly in food, pharmaceutical and biomedical fields. At present bacterial cellulose is considered as a perspective material for obtaining of a wide range of nanostructured biomaterials and biocomposites. The goal of our work was to study bacterial cellulose and xanthan production by novel efficient strains using food industry wastes. Gluconacetobacter sucrofermentans and Xanthomonas campestris bacteria cultivation conditions were optimized. The balanced medium provides high yield of bacterial cellulose (7 g/l) and xanthan (25 g/l) and contributes to industrial wastes utilization. The physicochemical properties of bacterial cellulose and xanthan were investigated. http://dx.doi.org/10.1016/j.jbiotec.2014.07.117 Stem Cells, Biomaterials, Tissue Engineering Plasmid DNA microgels for cancer treatment through combination of chemotherapy and gene delivery Diana Barata Costa 1,∗ , Artur Monteiro Valente 2 , João Sampaio Queiroz 1
Piyanun Wangkulangkul 1,∗ , Jirayut Jaipaew 1 , Jirut Meesane 1 , Supaporn Krivimol 2 , Puttisak Puttawibul 1 1
Institute of Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hatyai, Songkla, Thailand 2 Queen Sirikit Sericulture Center, Narathiwat, Thailand E-mail address: neoz [email protected] (P. Wangkulangkul). Porous silk scaffolds prepared by the salt-leaching technique are often used in soft tissue engineering. However, this scaffold type is stiffer with less surface area than porous silk scaffolds prepared by other methods. In this study, we produced reinforced salt-leaching porous scaffolds by soaking the salt-leached scaffolds in 0.125%, 0.25%, 0.5% and 1% (w/v) of fibroin solutions and then they were lyophilized. The scanning electron micrograph of 0.5% and 1.0% reinforced scaffolds showed a net-like structure in each scaffold pore. The 0.25% reinforced scaffolds demonstrated interconnecting bridges in the scaffold pores and the 0.125% reinforced scaffold illustrated a thicker scaffold wall than the native salt-leaching scaffold. Moreover, the reinforced group was significantly more stable than the native scaffolds. Furthermore, the compressive moduli of 0.125%, 0.25% and 0.5% reinforcements were less than the native. However, the compressive modulus of the 1.0% reinforcement was not significantly different from the native. For biomedical applications, we investigated the feasibility of adipose tissue engineering on a reinforced scaffold by culturing the pre-adipocyte 3T3-L1 cell line and evaluated their proliferation in the scaffolds. The results showed that all scaffolds were not toxic and the proliferation of the 3T3-L1 cell in all scaffolds was not significantly different.
1
CICS-UBI – Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal 2 Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal E-mail address: [email protected] (D.B. Costa).
Plasmid DNA (pDNA) was efficiently encapsulated into biocompatible microgels by an inverse microemulsion polymerization method using ethylene glycol diglycidyl ether (EGDE) as cross-linker. Plasmid DNA and an anticancer drug, doxorubicin (DOX), could both be loaded with high encapsulation efficiency and released from pDNA microgels; quite relevant is also the less toxic effect of the incorporated drug, as compared with naked DOX. Photodisruption of microgels can be used as a strategy to enhance release. Mathematical models were applied to fully characterize the pDNA and drug release profiles. The delivery of the encapsulated pDNA shows the capability of cell internalization and transfection in vitro resulting in the expression of the p53 protein. The effect of this system on cell viability inhibition was evaluated in cancer Hela cells. The treatment with a pDNA/doxorubicin loaded microgel improves cell apoptosis. Compared with pDNA microgel or free drug, the co-delivery system has a stronger potential to suppress the development of cancer cells. This pDNA photodegradable system can
http://dx.doi.org/10.1016/j.jbiotec.2014.07.119 Natural-based polymer scaffolds with microwave induced porosity Antonella Giuri 1 , Vincenzo Maria De Benedictis 2 , Maria Grazia Raucci 3 , Christian Demitri 1,∗ , Alessndro Sannino 1 1 University of Salento, Department of Innovation for Engineering, Via Monteroni, Campus Ecotekne, 73100 Lecce, Italy 2 High Tech District – DHITECH Scarl, via S. Trinchese, 61, 73100 Lecce, Italy 3 Institute of Polymers, Composites and Biomaterials, National Research Council of Italy (IPCB-CNR), Italy
E-mail address: [email protected] (C. Demitri). In this work, interconnected porous material was obtained by a new foaming method based on the combination of two steps. Initially, a stable physical foaming was induced using a surfactant (i.e. pluronic) as blowing agent of a homogeneous blend of polysaccharide and polyethylene glycol diacrylate solution. Furthermore, the porous structure of the foam was chemically stabilized by radical polymerization induced by homogeneous heating of the sample