- Email: [email protected]
Bioactive polyphenols and flavonoids in cell cultures of hops (Humulus lupulus L.)
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
families and the natural habitat, more or less close to the sea, of different populations. Initial results will be presented and discussed. doi:10.1016/j.copbio.2011.05.464
P41 Effects of jasmonic acid and salt applications on antioxydative enzyme activities of the eggplant seedlings grown in vitro culture Banu Gunalp 1 , Ozge Horasan 1 , Fikret Yasar 2 , Sebnem Kusvuran 3 , Rukiye Tipirdamaz 4 , Sebnem Ellialtioglu 1 1
Department of Horticulture, Faculty of Agriculture, University of Ankara, Ankara, Turkey 2 Department of Horticulture, Faculty of Agriculture, University of Yuzuncu Yil, Van, Turkey 3 Kizilirmak Vocational High School, University of Cankiri Karatekin, Cankiri, Turkey 4 Department of Biology, Faculty of Science, University of Hacettepe, Ankara, Turkey
S141
fering in growth regulator contents. Cell suspension cultures were established from the respective callus cultures in liquid media containing half the concentrations of growth regulators. Samples were taken from both calli and cell suspension cultures for chemical analyses. Explant type, culture condition, medium composition as well as genotype all affected total polyphenol and flavonoid contents in hop in vitro cultures. Using HPLC xanthohumol could be detected in hop cell suspension cultures. In vitro cultures of hops contained bioactive polyphenols and flavonoids, including xanthohumol. doi:10.1016/j.copbio.2011.05.466
P43 The comparison of the roles of total phenolic compounds against UV-A stress between two macro algae Ulva lactuca (Chlorophyta) and Patelonia sp. (Phaeophyta) Inci Tuney 1 , Dilek Unal 2 , Zeliha Demirel 1 , Atakan Sukatar 1 1 2
Ege Universitesi, Izmir, Turkey Bilecik Universitesi, Bilecik, Turkey
E-mail address: [email protected] (S. Kusvuran)
E-mail address: [email protected] (I. Tuney)
The aim of this study was to investigate the effects of exogenous application of JA on salt tolerance of eggplant embryos cultured in vitro. As growth medium MS media was used (Control) and 10 (M JA, 20 (M JA, 100 mM NaCl, 10 (M JA + 100 mM NaCl, 20 (M JA + 100 mM NaCl, 10 (M JA (pre-treatment) + 100 mM NaCl and 20 (M JA (pre-treatment) + 100 mM NaCl were added to the media. Thirty-two days old embryos of Kemer and Mardin eggplant genotypes were planted in eight different growth media. Germination rate was 100% and plant growth was normal in control application however germination rate and plant growth were inhibited by salt or JA application. The addition of JA caused more inhibition than salt stress. Among all applications of JA, plants that were pretreated with 10 (M JA and then transferred to the growth media with salt showed relatively better development than the others. Antioxydative enzymes, SOD, CAT and APX, increased with salt or JA treatments compared to control plants. The SOD, CAT and APX activities of the salt-tolerant Mardin genotype were higher than salt-sensitive Kemer variety
Ulva lactuca and Patelonia sp. were exposed to UV-A (420–320 nm) for 30 min, 1 h, and 2 h, to investigate the alterations in the PSII photosynthetic quantum yield, and phenolic compounds content in response to radiation. Significant alterations of the photosynthetic quantum yield (Fv/Fm) ratio occurred in response to increase in UV-A exposure time. The Fv/Fm ratio decreased in U. lactuca following exposure to UV-A for 1 h. We also showed that lipid peroxidation levels between UV-A-treated samples and nontreated samples that were previously exposed to UV-A for 1 h and 2 h were significantly increased in U. lactuca thalli. However, the Fv/Fm ratio in Patelonia following exposure to UV-A for 30 min, 1 h and 2 h were not significantly changed. Although total phenolic compound content was gradually increased in Patelonia thalli exposed to UV-A for 30 min, 1 h and 2 h, in thalli of U. lactuca was significantly decreased. Our experiment showed that it is correlation between phenolic content and Fv/Fm ratio under UV-A stress.
doi:10.1016/j.copbio.2011.05.465
P42 Bioactive polyphenols and flavonoids in cell cultures of hops (Humulus lupulus L.) Juraj Farago, Filip Kluciarovsky, Ivana Psenakova Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic E-mail address: [email protected] (J. Farago) Hops (Humulus lupulus L.) are traditionally known as the raw material in the brewing industry. Recently, some bioactive compounds from hops, especially prenylflavonoids, have received much attention because of their broad spectrum biological activities. In vitro culture systems could provide a feasible model system to study the biosynthesis and accumulation of these compounds in cells as well as an alternative production system. Our aim is to establish a convenient in vitro system to study the chemical composition of hop in vitro cultures. Callus cultures were established from in vitro grown plants of two hop mericlones (K72/6/13, PRM/3) using internodal and leaf explants cultured on two MS media dif-
doi:10.1016/j.copbio.2011.05.467
P44 Biotechnology for abiotic tolerance and nutritional improvement in selected genotypes of Solanum melongena and Cucurbita moschata Carmina Gisbert 1 , Jose Miguel Mulet 2 , Jaime Prohens 1 , Belen Pico 1 , Ramon Serrano 2 , Fernando Nuez 1 1
Institute for the Conservation and Breeding of Agricultural Biodiversity (COMAV), Universitat Politecnica de Valencia, Valencia, Spain 2 Instituto de Biologia Molecular y Celular de Plantas (IBMCP), Valencia, Spain E-mail address: [email protected] (C. Gisbert) Tolerances to biotic and abiotic stresses as well as improving crop nutritional value are currently considered main breeding objectives. The goal of this work is to introduce the BvSAT gene that codifies a serine acetyl transferase, one of the enzymes responsible for the biosynthesis of the essential aminoacids cysteine and methionine, in selected gentoypes. Concretely, into a genotype of Solanum melongena previously selected for its Meloidogyne incognita tolerance, and into a Cucurbita moschata genotype selected for its tolerance to viruses and fungi, and for its fruit quality. This
families and the natural habitat, more or less close to the sea, of different populations. Initial results will be presented and discussed. doi:10.1016/j.copbio.2011.05.464
P41 Effects of jasmonic acid and salt applications on antioxydative enzyme activities of the eggplant seedlings grown in vitro culture Banu Gunalp 1 , Ozge Horasan 1 , Fikret Yasar 2 , Sebnem Kusvuran 3 , Rukiye Tipirdamaz 4 , Sebnem Ellialtioglu 1 1
Department of Horticulture, Faculty of Agriculture, University of Ankara, Ankara, Turkey 2 Department of Horticulture, Faculty of Agriculture, University of Yuzuncu Yil, Van, Turkey 3 Kizilirmak Vocational High School, University of Cankiri Karatekin, Cankiri, Turkey 4 Department of Biology, Faculty of Science, University of Hacettepe, Ankara, Turkey
S141
fering in growth regulator contents. Cell suspension cultures were established from the respective callus cultures in liquid media containing half the concentrations of growth regulators. Samples were taken from both calli and cell suspension cultures for chemical analyses. Explant type, culture condition, medium composition as well as genotype all affected total polyphenol and flavonoid contents in hop in vitro cultures. Using HPLC xanthohumol could be detected in hop cell suspension cultures. In vitro cultures of hops contained bioactive polyphenols and flavonoids, including xanthohumol. doi:10.1016/j.copbio.2011.05.466
P43 The comparison of the roles of total phenolic compounds against UV-A stress between two macro algae Ulva lactuca (Chlorophyta) and Patelonia sp. (Phaeophyta) Inci Tuney 1 , Dilek Unal 2 , Zeliha Demirel 1 , Atakan Sukatar 1 1 2
Ege Universitesi, Izmir, Turkey Bilecik Universitesi, Bilecik, Turkey
E-mail address: [email protected] (S. Kusvuran)
E-mail address: [email protected] (I. Tuney)
The aim of this study was to investigate the effects of exogenous application of JA on salt tolerance of eggplant embryos cultured in vitro. As growth medium MS media was used (Control) and 10 (M JA, 20 (M JA, 100 mM NaCl, 10 (M JA + 100 mM NaCl, 20 (M JA + 100 mM NaCl, 10 (M JA (pre-treatment) + 100 mM NaCl and 20 (M JA (pre-treatment) + 100 mM NaCl were added to the media. Thirty-two days old embryos of Kemer and Mardin eggplant genotypes were planted in eight different growth media. Germination rate was 100% and plant growth was normal in control application however germination rate and plant growth were inhibited by salt or JA application. The addition of JA caused more inhibition than salt stress. Among all applications of JA, plants that were pretreated with 10 (M JA and then transferred to the growth media with salt showed relatively better development than the others. Antioxydative enzymes, SOD, CAT and APX, increased with salt or JA treatments compared to control plants. The SOD, CAT and APX activities of the salt-tolerant Mardin genotype were higher than salt-sensitive Kemer variety
Ulva lactuca and Patelonia sp. were exposed to UV-A (420–320 nm) for 30 min, 1 h, and 2 h, to investigate the alterations in the PSII photosynthetic quantum yield, and phenolic compounds content in response to radiation. Significant alterations of the photosynthetic quantum yield (Fv/Fm) ratio occurred in response to increase in UV-A exposure time. The Fv/Fm ratio decreased in U. lactuca following exposure to UV-A for 1 h. We also showed that lipid peroxidation levels between UV-A-treated samples and nontreated samples that were previously exposed to UV-A for 1 h and 2 h were significantly increased in U. lactuca thalli. However, the Fv/Fm ratio in Patelonia following exposure to UV-A for 30 min, 1 h and 2 h were not significantly changed. Although total phenolic compound content was gradually increased in Patelonia thalli exposed to UV-A for 30 min, 1 h and 2 h, in thalli of U. lactuca was significantly decreased. Our experiment showed that it is correlation between phenolic content and Fv/Fm ratio under UV-A stress.
doi:10.1016/j.copbio.2011.05.465
P42 Bioactive polyphenols and flavonoids in cell cultures of hops (Humulus lupulus L.) Juraj Farago, Filip Kluciarovsky, Ivana Psenakova Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic E-mail address: [email protected] (J. Farago) Hops (Humulus lupulus L.) are traditionally known as the raw material in the brewing industry. Recently, some bioactive compounds from hops, especially prenylflavonoids, have received much attention because of their broad spectrum biological activities. In vitro culture systems could provide a feasible model system to study the biosynthesis and accumulation of these compounds in cells as well as an alternative production system. Our aim is to establish a convenient in vitro system to study the chemical composition of hop in vitro cultures. Callus cultures were established from in vitro grown plants of two hop mericlones (K72/6/13, PRM/3) using internodal and leaf explants cultured on two MS media dif-
doi:10.1016/j.copbio.2011.05.467
P44 Biotechnology for abiotic tolerance and nutritional improvement in selected genotypes of Solanum melongena and Cucurbita moschata Carmina Gisbert 1 , Jose Miguel Mulet 2 , Jaime Prohens 1 , Belen Pico 1 , Ramon Serrano 2 , Fernando Nuez 1 1
Institute for the Conservation and Breeding of Agricultural Biodiversity (COMAV), Universitat Politecnica de Valencia, Valencia, Spain 2 Instituto de Biologia Molecular y Celular de Plantas (IBMCP), Valencia, Spain E-mail address: [email protected] (C. Gisbert) Tolerances to biotic and abiotic stresses as well as improving crop nutritional value are currently considered main breeding objectives. The goal of this work is to introduce the BvSAT gene that codifies a serine acetyl transferase, one of the enzymes responsible for the biosynthesis of the essential aminoacids cysteine and methionine, in selected gentoypes. Concretely, into a genotype of Solanum melongena previously selected for its Meloidogyne incognita tolerance, and into a Cucurbita moschata genotype selected for its tolerance to viruses and fungi, and for its fruit quality. This