- Email: [email protected]
Biochemical engineering studies in microbial culture processes
VOL. 82, 1996
Abstracts of the Articles Printed in Seibutsu-kogaku Kaishi Vol. 74, No. 6 (1996) butanol and water layers exhibited I-N-acetylglucosaminidase inhibition. The inhibitor contained in the water layer was partially purified by successive column chromatography with active carbon and ion exchanger AGSOW-X8. The purified inhibitor was active on various sources of B-N-acetylglucosaminidase such as Streptomyces sp., beans and abalone entrails, and kidney bean chitobiase, but had no effect on chitinases from Sreptomyces antibioticus and Sreptomyces griseus.
Continuous Production of tPA by Recombinant BHK Cells in a Micro-Filtration Hollow Fiber Bloreactor. TAKA~KI T~~E,‘~~* Y~uuoat Irrno~,’ Krvosrir Iwm,’ AKnu (Eisai b.%XIM0T0,‘T~uumnx o KATAOKA,' and T~gasnt Ko~Y~~ Co. Ltd., S-l-3 Tokodai, Tsukuba 3-26 and Department of Biotechnology, Faculty of Engineering, Nagoya University, Chikusa-ku, Nagoya 466Ol*) Seibutsu-kogaku 14: 435441. 1996. Continuous cultivation of recombinant Baby Hamster Kidney (BHK) cells producing tissue plasminogen activator (tPA) was carried out in a micro-filtration hollow fiber (MFHF) bioreactor using Dulbecco’s Modified Eagle’s medium with serum. Stable, continuous cultivation for 66 d was possible, compared with a stoppage of cultivation at 40 d in the case of an ultra-hltration hollow fiber (UFHF) bioreactor. After the cells were fully grown, the serum concentration was decreased from 5% to lx, but the tPA productivity remained at the same level continuously. The mean tPA concentration was 17.1 mg/l and the mean daily tPA production was 59Omg/d. The tPA productivity was 150 mg/d/mz in the case of the MFHF bioreactor. As the medium in the MFHF bioreactor was evenly supplied from the intra-capillary to the extra-capillary space, the tPA productivity of this bioreactor was 16% better than that of the UFHF type. The simplified culture system of the MFHF bioreactor make it suitable for commercial-scale tPA production.
* Corresponding author. Biochemical Engineering Studies in Microbial Culture Processes. -Personal Consequence24-0 NAGAI (Yaegaki Technology Development Laboratories, Yaegaki Bio-Industry Inc., 681 Mukudani, Hayashida-cho, Himeji 679-42) Seibutsu-kokagu 74: 447-455. 1996. Here, personal research consequences which have been carried out for more than 30 years at Osaka Univ., The Univ. of Tokyo and Hiroshima Univ. are partly outlined as follows: 1. Studies on filtration and press-dehydration of fermentation mashes, 2. Dynamical analysis of microbial growth, 3. Fed batch culture of Saccharomyces cerevisiae with computer control, 4. Structure and function of methane fermentation; recent progress of anaerobic wastewater treatments, and 5. Vitamin B,* production by methanogens.
* Corresponding author. Properties of Phospholipase B from the Yeast Torulaspora delbrueckti and Its Physiological Effects on the Durabiity of the Yeast. -ReviewYou~cm TM* and YGUO WATANABE (Department of Bioresources, Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790) Seibutsu-kogaku 74: 457468. 1996. Phospholipid deacylating enzymes of yeast have been studied primarily in Saccharomyces cerevisiae, a baker’s yeast, and Candida albicans, a pathogenic yeast. In recent years, the genes of S. cerevisiae and Toruiaspora delbrueckii have been cloned, providing detailed data on the physiological roles, structure, and activity of these enzymes in yeast. We report here on the results of our study on phospholipid deacylating enzymes, mainly the enzyme derived from T. delbrueckii, and discuss the physiological role of this enzyme in the cells of this yeast.
Production of ,9-N-Acetylglucosaminidase Inhibitor by Screened Microorganisms and Properties of the Inhibitor. -NoteKORNO, YASUYUKI Tonau IWAMOTO,* C-u Iss~mr, T= Fusuow, and Yosntmao SHUTO (Department of Bioresources Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790) Seibutsu-kogaku 74: 4434%. 19%. Seven strains which produced B-N-acetylglucosaminidase inhibitors were obtained by using the Streptomyces enzyme. The inhibitors produced by these strains suppressed not only Streptomyces /3-Nacetylglucosaminidase but also the enzyme from other sources, including plant and animal sources, to some extent. An Actinomycetes strain was selected from among the 7 strains as a potent inhibitor producer and used for subsequent experiments. Of the various carbon sources in Benett medium, N-acetylchitooligosaccharide and colloidal chitin markedly promoted production of the inhibitor. The culture broth was extracted with ethylacetate and n-butanol. Both the
* Corresponding author.
620
Abstracts of the Articles Printed in Seibutsu-kogaku Kaishi Vol. 74, No. 6 (1996) butanol and water layers exhibited I-N-acetylglucosaminidase inhibition. The inhibitor contained in the water layer was partially purified by successive column chromatography with active carbon and ion exchanger AGSOW-X8. The purified inhibitor was active on various sources of B-N-acetylglucosaminidase such as Streptomyces sp., beans and abalone entrails, and kidney bean chitobiase, but had no effect on chitinases from Sreptomyces antibioticus and Sreptomyces griseus.
Continuous Production of tPA by Recombinant BHK Cells in a Micro-Filtration Hollow Fiber Bloreactor. TAKA~KI T~~E,‘~~* Y~uuoat Irrno~,’ Krvosrir Iwm,’ AKnu (Eisai b.%XIM0T0,‘T~uumnx o KATAOKA,' and T~gasnt Ko~Y~~ Co. Ltd., S-l-3 Tokodai, Tsukuba 3-26 and Department of Biotechnology, Faculty of Engineering, Nagoya University, Chikusa-ku, Nagoya 466Ol*) Seibutsu-kogaku 14: 435441. 1996. Continuous cultivation of recombinant Baby Hamster Kidney (BHK) cells producing tissue plasminogen activator (tPA) was carried out in a micro-filtration hollow fiber (MFHF) bioreactor using Dulbecco’s Modified Eagle’s medium with serum. Stable, continuous cultivation for 66 d was possible, compared with a stoppage of cultivation at 40 d in the case of an ultra-hltration hollow fiber (UFHF) bioreactor. After the cells were fully grown, the serum concentration was decreased from 5% to lx, but the tPA productivity remained at the same level continuously. The mean tPA concentration was 17.1 mg/l and the mean daily tPA production was 59Omg/d. The tPA productivity was 150 mg/d/mz in the case of the MFHF bioreactor. As the medium in the MFHF bioreactor was evenly supplied from the intra-capillary to the extra-capillary space, the tPA productivity of this bioreactor was 16% better than that of the UFHF type. The simplified culture system of the MFHF bioreactor make it suitable for commercial-scale tPA production.
* Corresponding author. Biochemical Engineering Studies in Microbial Culture Processes. -Personal Consequence24-0 NAGAI (Yaegaki Technology Development Laboratories, Yaegaki Bio-Industry Inc., 681 Mukudani, Hayashida-cho, Himeji 679-42) Seibutsu-kokagu 74: 447-455. 1996. Here, personal research consequences which have been carried out for more than 30 years at Osaka Univ., The Univ. of Tokyo and Hiroshima Univ. are partly outlined as follows: 1. Studies on filtration and press-dehydration of fermentation mashes, 2. Dynamical analysis of microbial growth, 3. Fed batch culture of Saccharomyces cerevisiae with computer control, 4. Structure and function of methane fermentation; recent progress of anaerobic wastewater treatments, and 5. Vitamin B,* production by methanogens.
* Corresponding author. Properties of Phospholipase B from the Yeast Torulaspora delbrueckti and Its Physiological Effects on the Durabiity of the Yeast. -ReviewYou~cm TM* and YGUO WATANABE (Department of Bioresources, Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790) Seibutsu-kogaku 74: 457468. 1996. Phospholipid deacylating enzymes of yeast have been studied primarily in Saccharomyces cerevisiae, a baker’s yeast, and Candida albicans, a pathogenic yeast. In recent years, the genes of S. cerevisiae and Toruiaspora delbrueckii have been cloned, providing detailed data on the physiological roles, structure, and activity of these enzymes in yeast. We report here on the results of our study on phospholipid deacylating enzymes, mainly the enzyme derived from T. delbrueckii, and discuss the physiological role of this enzyme in the cells of this yeast.
Production of ,9-N-Acetylglucosaminidase Inhibitor by Screened Microorganisms and Properties of the Inhibitor. -NoteKORNO, YASUYUKI Tonau IWAMOTO,* C-u Iss~mr, T= Fusuow, and Yosntmao SHUTO (Department of Bioresources Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790) Seibutsu-kogaku 74: 4434%. 19%. Seven strains which produced B-N-acetylglucosaminidase inhibitors were obtained by using the Streptomyces enzyme. The inhibitors produced by these strains suppressed not only Streptomyces /3-Nacetylglucosaminidase but also the enzyme from other sources, including plant and animal sources, to some extent. An Actinomycetes strain was selected from among the 7 strains as a potent inhibitor producer and used for subsequent experiments. Of the various carbon sources in Benett medium, N-acetylchitooligosaccharide and colloidal chitin markedly promoted production of the inhibitor. The culture broth was extracted with ethylacetate and n-butanol. Both the
* Corresponding author.
620