- Email: [email protected]
Biofilm formation by Microsporum canis
Accepted Manuscript Biofilm formation by Microsporum canis L.J. Danielli, W. Lopes, M.H. Vainstein, A.M. Fuentefria, M.A. Apel PII:
S1198-743X(17)30325-7
DOI:
10.1016/j.cmi.2017.06.006
Reference:
CMI 973
To appear in:
Clinical Microbiology and Infection
Received Date: 23 March 2017 Revised Date:
5 June 2017
Accepted Date: 6 June 2017
Please cite this article as: Danielli LJ, Lopes W, Vainstein MH, Fuentefria AM, Apel MA, Biofilm formation by Microsporum canis, Clinical Microbiology and Infection (2017), doi: 10.1016/ j.cmi.2017.06.006. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT Biofilm formation by Microsporum canis
1
2
L.J. Danielli1, W. Lopes2, M.H. Vainstein2, A.M. Fuentefria1, M.A. Apel1*
4
1
5
Grande do Sul, Brasil
6
2
7
*Corresponding author: M. A. Apel, Faculdade de Farmácia, Universidade Federal do
8
Rio Grande do Sul, Av. Ipiranga, 2752, 90610-000, Porto Alegre, Brazil. Tel.: +55 51
9
3308 5258. Fax: +55 51 3308 5417 E- mail address: [email protected]
RI PT
3
Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio
M AN U
SC
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Brasil
Manuscript category: Picture of a Microorganism
11
Keywords: Biofilm, Dermatophytes, Microsporum canis, SEM, Tinea capitis.
12
Text
EP
TE D
10
Biofilm formation is an important cause of failures in antimicrobial therapy usually
14
associated to the increasing of resistance to antimicrobial agents and to host immune
15
response. The ability to form such structures has been described for several fungal species
16
however the biofilm formation by dermatophytes was reported only for Trichophyton spp.
17
[1]. In this study, we described for the first time the biofilm formation by Microsporum
18
canis, considered as one of the main etiological agents of tinea capitis and characterized by
19
a poor therapeutic response. Biofilm was formed in glass coverslips after 120 hours of
20
culturing and observed by scanning electron microscopy (SEM) from a clinical sample of
21
M. canis.
AC C
13
ACCEPTED MANUSCRIPT The images obtained by SEM demonstrate the presence of a highly structured three-
2
dimensional mycelium characteristic of biofilm (Fig. 1 A-B) with expansion in the form of
3
a network of hyphae growing in all directions (Fig. 1 C-D). Moreover, polysaccharide
4
extracellular matrix that links one hyphae to another is observed surrounding some areas of
5
this mycelium structure. The presence of polysaccharide was confirmed by reaction with
6
Periodic Acid-Schiff (PAS) (Fig. 1 E-F). The extracellular matrix presents compact
7
regions, but usually with a porous and thin consistency, concentrated in the superficial
8
region of the mycelium. Formation of biofilm by dermatophytes can be considered as an
9
important factor of fungal virulence, which may be involved with the persistence of the
10
infection. So, this finding contribute perspectives for effective therapy to eradicate
11
infections related to biofilm formation by dermatophytes.
M AN U
SC
RI PT
1
12
14
Financial support This
work
TE D
13
was
supported
by
Brazilian
organizations
Coordenação
de
Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de
16
Desenvolvimento Científico e Tecnológico (CNPq). A. M. Fuentefria, M. A. Apel and M.
17
H. Vainstein are grateful to CNPq for the PQ fellowships.
19
20
AC C
18
EP
15
Transparency declaration The authors report no conflicts of interest.
21
22
Author’s contribution
ACCEPTED MANUSCRIPT L.J. Danielli - drafting the manuscript, literature review and laboratory work.
2
W. Lopes - drafting the manuscript, literature review and laboratory work.
3
M.H. Vainstein - drafting the manuscript and literature review.
4
A.M. Fuentefria - drafting the manuscript and literature review.
5
M.A. Apel - drafting the manuscript and literature review.
SC
References
M AN U
6
7
RI PT
1
8
[1] Costa-Orlandi CB, Sardi JCO, Santos CT, Fusco-Almeida AM, Mendes-Giannini MJS. In
9
vitro characterization of Trichophyton rubrum and T. mentagrophytes biofilms.
EP
TE D
Biofouling 2014;30:719–27.
AC C
10
AC C
EP
TE D
M AN U
SC
RI PT
ACCEPTED MANUSCRIPT
S1198-743X(17)30325-7
DOI:
10.1016/j.cmi.2017.06.006
Reference:
CMI 973
To appear in:
Clinical Microbiology and Infection
Received Date: 23 March 2017 Revised Date:
5 June 2017
Accepted Date: 6 June 2017
Please cite this article as: Danielli LJ, Lopes W, Vainstein MH, Fuentefria AM, Apel MA, Biofilm formation by Microsporum canis, Clinical Microbiology and Infection (2017), doi: 10.1016/ j.cmi.2017.06.006. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT Biofilm formation by Microsporum canis
1
2
L.J. Danielli1, W. Lopes2, M.H. Vainstein2, A.M. Fuentefria1, M.A. Apel1*
4
1
5
Grande do Sul, Brasil
6
2
7
*Corresponding author: M. A. Apel, Faculdade de Farmácia, Universidade Federal do
8
Rio Grande do Sul, Av. Ipiranga, 2752, 90610-000, Porto Alegre, Brazil. Tel.: +55 51
9
3308 5258. Fax: +55 51 3308 5417 E- mail address: [email protected]
RI PT
3
Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio
M AN U
SC
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Brasil
Manuscript category: Picture of a Microorganism
11
Keywords: Biofilm, Dermatophytes, Microsporum canis, SEM, Tinea capitis.
12
Text
EP
TE D
10
Biofilm formation is an important cause of failures in antimicrobial therapy usually
14
associated to the increasing of resistance to antimicrobial agents and to host immune
15
response. The ability to form such structures has been described for several fungal species
16
however the biofilm formation by dermatophytes was reported only for Trichophyton spp.
17
[1]. In this study, we described for the first time the biofilm formation by Microsporum
18
canis, considered as one of the main etiological agents of tinea capitis and characterized by
19
a poor therapeutic response. Biofilm was formed in glass coverslips after 120 hours of
20
culturing and observed by scanning electron microscopy (SEM) from a clinical sample of
21
M. canis.
AC C
13
ACCEPTED MANUSCRIPT The images obtained by SEM demonstrate the presence of a highly structured three-
2
dimensional mycelium characteristic of biofilm (Fig. 1 A-B) with expansion in the form of
3
a network of hyphae growing in all directions (Fig. 1 C-D). Moreover, polysaccharide
4
extracellular matrix that links one hyphae to another is observed surrounding some areas of
5
this mycelium structure. The presence of polysaccharide was confirmed by reaction with
6
Periodic Acid-Schiff (PAS) (Fig. 1 E-F). The extracellular matrix presents compact
7
regions, but usually with a porous and thin consistency, concentrated in the superficial
8
region of the mycelium. Formation of biofilm by dermatophytes can be considered as an
9
important factor of fungal virulence, which may be involved with the persistence of the
10
infection. So, this finding contribute perspectives for effective therapy to eradicate
11
infections related to biofilm formation by dermatophytes.
M AN U
SC
RI PT
1
12
14
Financial support This
work
TE D
13
was
supported
by
Brazilian
organizations
Coordenação
de
Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de
16
Desenvolvimento Científico e Tecnológico (CNPq). A. M. Fuentefria, M. A. Apel and M.
17
H. Vainstein are grateful to CNPq for the PQ fellowships.
19
20
AC C
18
EP
15
Transparency declaration The authors report no conflicts of interest.
21
22
Author’s contribution
ACCEPTED MANUSCRIPT L.J. Danielli - drafting the manuscript, literature review and laboratory work.
2
W. Lopes - drafting the manuscript, literature review and laboratory work.
3
M.H. Vainstein - drafting the manuscript and literature review.
4
A.M. Fuentefria - drafting the manuscript and literature review.
5
M.A. Apel - drafting the manuscript and literature review.
SC
References
M AN U
6
7
RI PT
1
8
[1] Costa-Orlandi CB, Sardi JCO, Santos CT, Fusco-Almeida AM, Mendes-Giannini MJS. In
9
vitro characterization of Trichophyton rubrum and T. mentagrophytes biofilms.
EP
TE D
Biofouling 2014;30:719–27.
AC C
10
AC C
EP
TE D
M AN U
SC
RI PT
ACCEPTED MANUSCRIPT