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Biological characterization of eel c-reative protein
Vlll
no cross reaction with mouse Ia antigen. The former lymphocyte occurred mainly in the spleen and liver, whereas the latter was found in the thymus and kidney; antibody producing cells were observed in the kidney. In blood lymphoeytes the former comprised 84-95%, the latter 516%. We suggest that the development of both lymphocyte types is important to each organ in generating immune aespones. 23. IMMUNE RESPONSE IN RAINBOW TROUT, ONCORHYNCHUS MYKISS, VACCINATED WITH p-HAEMOLYTIC STREPTOCOCCAL BACTERIN. M. Sakai, S. Atsuta and M. Kobayashi. SchoolofFisheriesSciences, Kitasato University,Iwate 022-01, Japan. Immunity against Streptoc~,cal disease has been investigated in the rainbow trout, Oncorhynchus mykiss by immersion in or intraperitoneal injection of formalin-killed bacteria. Groups of 25 fish immunized by immersion as well as injection showed 70% or greater relative per cent survival (RPS) when challenged with live Streptococcus sp. No agglutinating antibodies were detected in the serum of fish vaccinated by the immersion method, although antibodies were detected in the serum of fish vaccinated by injection. Bactericidal activities of the serum in fish vaccinated by either the immersion or injection method did not increase. Phagocytic activities of kidney cells from vaccinated fish increased in the presence of normal fish sera. These responses were enhanced by adding sera from fish which had been vaccinated by injection or immersion.
24. COMPARISON OF PHAGOCYTOSIS AND CHEMILUMINESCENT RESPONSE OF LEUCOCYTES FROM FISH AND HUMAN TO STREPTOCOCCUS SP. KG" AND KG + STRAIN. T. Kitao, T. Eshima and T. Yoshida. DeparOnent of Fisheries, Faculty of
Agriculture, Miyazaki University, Miyazaki 88921, Japan. It has been reported that Streptococcus sp., the causative agent of cultured yellowtail (Seriola quinqueradiata) possessed the variation from KG" type (virulent strain) to KG + type (avirulent strain). Phagocytic activity and chemiluminescent (CL) responses of leucocytes in two fish species, yellowtail and rainbow trout (Oncorhyncus mykiss) as well as human were examined using Streptococcus sp. KG" and KG + strains which were opsonized either with fresh serum of yellowtail, rainbow trout or human, respectively. Leucocytes, isolated from fish
Abstracts
pronephros and human peripheral blood were used. Phagocytic activity of fish leucocytes to Streptococcus sp. KG" strain was twice lower than that to Streptococcus sp. KG + strain, whereas human leucocytes to KG- showed about 40 times lower activity than that to Streptococcus sp. KG + strain. Similarly CL response of fish leucocytes stimulated with Streptococcus sp. KG'strain was about half of that with Streptococcus sp. KG + strain, and human leucocytes with KG'showed about 4 times lower activity than with KG ÷ strain. BIOLOGICAL CHARACTERIZATION OF EEL C-REACTIVE PROTEIN. W. Nunomura. Tumour Laboratory and Nippon 25.
Bio-Test Laboratory Inc., Kokubunj~ Tokyo 185, Japan. C-reactive protein (CRP) which was detectedwithin the albumin region on immunoelectrophoresls, was purified from Japanese eel (Anguillajaponica) serum. On SDS-PAGE, eel CRP (eCRP) migrated as a single band with a molecular weight (MW) of 24 kd under reducing conditions. The MW of a native eCRP was estimated to be 120 kd by Sephacryl S-300 gel filtration. The eCRP showed an agglutination activity against Streptococcus pneumoniae in the presence of Ca 2+. The eCRP agglutinated rabbit red blood cells, but not those of human, rat, guinea pig, horse, sheep nor goose. Hemagglutinating activity was inhibited by glucosamine or mannose. The eCRP formed a precipitin line with histone, protamine, poly-L-lysine and poly-Larginine in agarose gel. Both eCRP and human CRP (hCRP) reacted to protamine, and the precipitin iineswere completely fused. However, there was no antigenic identity between eCRP and hCRP by the Ouehterlony test using specific rabbit antiserum to eCRP and hCRP. Serum levels of eCRP were determined by electroimmunoassay and enzyme-immunoassay (ETA). These levels were distributed in a wide range (6.8 ng/ml - 5.3 mg/ml, n= 187), with a mean value of 834 ng/ml. 26. PRODUCTION OF MONOCLONAL ANTIBODIES SPECIFIC TO ALLOANTIGENS OF THE FISH, ORYZIAS LATIPES. T. Matsuzaki, M. Sakaizumi* and A. Shima.
Zoological Institute, Faculty of Science, University of Tokyo, Tokyo 113,Japan, and *The Tokyo Me~opolitan Institute of Medical Science, Bunkyo-la~ Tokyo 113, Japan. The number of major histoeompatibility loci in inbred strains of the medaka, OoMas /at/pes was determined by
no cross reaction with mouse Ia antigen. The former lymphocyte occurred mainly in the spleen and liver, whereas the latter was found in the thymus and kidney; antibody producing cells were observed in the kidney. In blood lymphoeytes the former comprised 84-95%, the latter 516%. We suggest that the development of both lymphocyte types is important to each organ in generating immune aespones. 23. IMMUNE RESPONSE IN RAINBOW TROUT, ONCORHYNCHUS MYKISS, VACCINATED WITH p-HAEMOLYTIC STREPTOCOCCAL BACTERIN. M. Sakai, S. Atsuta and M. Kobayashi. SchoolofFisheriesSciences, Kitasato University,Iwate 022-01, Japan. Immunity against Streptoc~,cal disease has been investigated in the rainbow trout, Oncorhynchus mykiss by immersion in or intraperitoneal injection of formalin-killed bacteria. Groups of 25 fish immunized by immersion as well as injection showed 70% or greater relative per cent survival (RPS) when challenged with live Streptococcus sp. No agglutinating antibodies were detected in the serum of fish vaccinated by the immersion method, although antibodies were detected in the serum of fish vaccinated by injection. Bactericidal activities of the serum in fish vaccinated by either the immersion or injection method did not increase. Phagocytic activities of kidney cells from vaccinated fish increased in the presence of normal fish sera. These responses were enhanced by adding sera from fish which had been vaccinated by injection or immersion.
24. COMPARISON OF PHAGOCYTOSIS AND CHEMILUMINESCENT RESPONSE OF LEUCOCYTES FROM FISH AND HUMAN TO STREPTOCOCCUS SP. KG" AND KG + STRAIN. T. Kitao, T. Eshima and T. Yoshida. DeparOnent of Fisheries, Faculty of
Agriculture, Miyazaki University, Miyazaki 88921, Japan. It has been reported that Streptococcus sp., the causative agent of cultured yellowtail (Seriola quinqueradiata) possessed the variation from KG" type (virulent strain) to KG + type (avirulent strain). Phagocytic activity and chemiluminescent (CL) responses of leucocytes in two fish species, yellowtail and rainbow trout (Oncorhyncus mykiss) as well as human were examined using Streptococcus sp. KG" and KG + strains which were opsonized either with fresh serum of yellowtail, rainbow trout or human, respectively. Leucocytes, isolated from fish
Abstracts
pronephros and human peripheral blood were used. Phagocytic activity of fish leucocytes to Streptococcus sp. KG" strain was twice lower than that to Streptococcus sp. KG + strain, whereas human leucocytes to KG- showed about 40 times lower activity than that to Streptococcus sp. KG + strain. Similarly CL response of fish leucocytes stimulated with Streptococcus sp. KG'strain was about half of that with Streptococcus sp. KG + strain, and human leucocytes with KG'showed about 4 times lower activity than with KG ÷ strain. BIOLOGICAL CHARACTERIZATION OF EEL C-REACTIVE PROTEIN. W. Nunomura. Tumour Laboratory and Nippon 25.
Bio-Test Laboratory Inc., Kokubunj~ Tokyo 185, Japan. C-reactive protein (CRP) which was detectedwithin the albumin region on immunoelectrophoresls, was purified from Japanese eel (Anguillajaponica) serum. On SDS-PAGE, eel CRP (eCRP) migrated as a single band with a molecular weight (MW) of 24 kd under reducing conditions. The MW of a native eCRP was estimated to be 120 kd by Sephacryl S-300 gel filtration. The eCRP showed an agglutination activity against Streptococcus pneumoniae in the presence of Ca 2+. The eCRP agglutinated rabbit red blood cells, but not those of human, rat, guinea pig, horse, sheep nor goose. Hemagglutinating activity was inhibited by glucosamine or mannose. The eCRP formed a precipitin line with histone, protamine, poly-L-lysine and poly-Larginine in agarose gel. Both eCRP and human CRP (hCRP) reacted to protamine, and the precipitin iineswere completely fused. However, there was no antigenic identity between eCRP and hCRP by the Ouehterlony test using specific rabbit antiserum to eCRP and hCRP. Serum levels of eCRP were determined by electroimmunoassay and enzyme-immunoassay (ETA). These levels were distributed in a wide range (6.8 ng/ml - 5.3 mg/ml, n= 187), with a mean value of 834 ng/ml. 26. PRODUCTION OF MONOCLONAL ANTIBODIES SPECIFIC TO ALLOANTIGENS OF THE FISH, ORYZIAS LATIPES. T. Matsuzaki, M. Sakaizumi* and A. Shima.
Zoological Institute, Faculty of Science, University of Tokyo, Tokyo 113,Japan, and *The Tokyo Me~opolitan Institute of Medical Science, Bunkyo-la~ Tokyo 113, Japan. The number of major histoeompatibility loci in inbred strains of the medaka, OoMas /at/pes was determined by