- Email: [email protected]
Biosensors for detection of pathogenic organisms
Journal of Biotechnology 136S (2008) S760–S762
Contents lists available at ScienceDirect
Journal of Biotechnology journal homepage: www.elsevier.com/locate/jbiotec
Abstracts
Section IX Biosafety and bioeconomy
IL-023 Stimulating the bioeconomy: Avoiding the pitfalls and obstacles in deploying sustainable biotechnology Alan McHughen UC Riverside, USA The impact of products of agricultural biotechnology since their commercial deployment in the 1990s has been measured by several governmental, academic and other sources. The primary impacts are seen in agriculture, particularly GM crops, which have made dramatic inroads in those developed and developing countries where they have been approved for cultivation. There are also impacts in non-food sectors, such as in the biofuels community. Lacking, however, are the considerations of the economic, social and environmental impacts of failing to deploy appropriate tools of agricultural biotechnology to fight hunger, malnutrition, poverty, environmental deterioration and other targets of opportunity.
of pathogenic organisms’ is a selective overview of molecular recognition elements currently impacting biosensing of pathogenic organisms. With the advent of nanostructures and new interface materials, these recognition elements will be major players in future pathogen detection biosensor development. Although transduction of the biorecognition event constitutes a separate and obvious important area of biosensor development, the focus of the presentation will be solely that of biosensor recognition element development important to the detection of pathogens. Acknowledgement US Army Contract W911SR-08-C-0024. Reference Chambers, J.P., Arulanandam, B.P., Matta, L.L., Weis, A., Valdes, J.J., 2008. Biosensor recognition elements. Curr. Issues Mol. Biol. 10, 1–12.
doi:10.1016/j.jbiotec.2008.07.1644
doi:10.1016/j.jbiotec.2008.07.1645
IL-044
IL-060
Biosensors for detection of pathogenic organisms James P.
Chambers 1 ,
James J.
Valdes 2,∗
1
Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA 2 Office of the Scientific Advisor for Biotechnology, Aberdeen Proving Ground, MD 21010, USA E-mail address: [email protected] (J.J. Valdes).
Molecular recognition is central to biosensing (Chambers et al., 2008). A biosensor can be defined as a ‘compact, analytical device incorporating a biological or biologically-derived sensitive ‘recognition’ element integrated or associated with a physiochemical transducer’. Initially, biosensor recognition elements were assumed to be isolated from a living system. However, many biosensor recognition elements now available are not naturally occurring but ones that have been synthesized in the laboratory. With the emergence of recombinatorially derived protein and nucleic acid constructs, generation of potentially useful sensor molecular recognition elements are arising from paths not even taken by nature. The presentation ‘Biosensors for detection
0168-1656/$ – see front matter
Nanoparticle-based strategies for high-performance biodetection Dun Pan, Juan Yan, Lihua Wang, Shiping Song, Chunhai Fan ∗ Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China E-mail address: [email protected] (C. Fan). The use of gold nanoparticles (AuNPs) has a long history in biology, dating back to the application of “immunogold” in biological imaging in the 1970’s. Based one the unique optical and electronic properties of AuNPs, a series of methods for ultrasensitive detection of DNA and proteins by using AuNPs have been developed. This has motivated intense interest to develop AuNPs-based biodetection. Here, I will present several examples from our group, demonstrating enhanced biodetection performance by exploiting nanoparticle–biomolecules interactions. In one of the studies, we interrogated the interactions between AuNPs and aptamer detection. Gold nanoparticles can effectively differentiate unstructured and folded DNA, thus providing a novel approach to probe nucleic acid structures. In the presence of
Contents lists available at ScienceDirect
Journal of Biotechnology journal homepage: www.elsevier.com/locate/jbiotec
Abstracts
Section IX Biosafety and bioeconomy
IL-023 Stimulating the bioeconomy: Avoiding the pitfalls and obstacles in deploying sustainable biotechnology Alan McHughen UC Riverside, USA The impact of products of agricultural biotechnology since their commercial deployment in the 1990s has been measured by several governmental, academic and other sources. The primary impacts are seen in agriculture, particularly GM crops, which have made dramatic inroads in those developed and developing countries where they have been approved for cultivation. There are also impacts in non-food sectors, such as in the biofuels community. Lacking, however, are the considerations of the economic, social and environmental impacts of failing to deploy appropriate tools of agricultural biotechnology to fight hunger, malnutrition, poverty, environmental deterioration and other targets of opportunity.
of pathogenic organisms’ is a selective overview of molecular recognition elements currently impacting biosensing of pathogenic organisms. With the advent of nanostructures and new interface materials, these recognition elements will be major players in future pathogen detection biosensor development. Although transduction of the biorecognition event constitutes a separate and obvious important area of biosensor development, the focus of the presentation will be solely that of biosensor recognition element development important to the detection of pathogens. Acknowledgement US Army Contract W911SR-08-C-0024. Reference Chambers, J.P., Arulanandam, B.P., Matta, L.L., Weis, A., Valdes, J.J., 2008. Biosensor recognition elements. Curr. Issues Mol. Biol. 10, 1–12.
doi:10.1016/j.jbiotec.2008.07.1644
doi:10.1016/j.jbiotec.2008.07.1645
IL-044
IL-060
Biosensors for detection of pathogenic organisms James P.
Chambers 1 ,
James J.
Valdes 2,∗
1
Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA 2 Office of the Scientific Advisor for Biotechnology, Aberdeen Proving Ground, MD 21010, USA E-mail address: [email protected] (J.J. Valdes).
Molecular recognition is central to biosensing (Chambers et al., 2008). A biosensor can be defined as a ‘compact, analytical device incorporating a biological or biologically-derived sensitive ‘recognition’ element integrated or associated with a physiochemical transducer’. Initially, biosensor recognition elements were assumed to be isolated from a living system. However, many biosensor recognition elements now available are not naturally occurring but ones that have been synthesized in the laboratory. With the emergence of recombinatorially derived protein and nucleic acid constructs, generation of potentially useful sensor molecular recognition elements are arising from paths not even taken by nature. The presentation ‘Biosensors for detection
0168-1656/$ – see front matter
Nanoparticle-based strategies for high-performance biodetection Dun Pan, Juan Yan, Lihua Wang, Shiping Song, Chunhai Fan ∗ Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China E-mail address: [email protected] (C. Fan). The use of gold nanoparticles (AuNPs) has a long history in biology, dating back to the application of “immunogold” in biological imaging in the 1970’s. Based one the unique optical and electronic properties of AuNPs, a series of methods for ultrasensitive detection of DNA and proteins by using AuNPs have been developed. This has motivated intense interest to develop AuNPs-based biodetection. Here, I will present several examples from our group, demonstrating enhanced biodetection performance by exploiting nanoparticle–biomolecules interactions. In one of the studies, we interrogated the interactions between AuNPs and aptamer detection. Gold nanoparticles can effectively differentiate unstructured and folded DNA, thus providing a novel approach to probe nucleic acid structures. In the presence of