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Biosynthesis and metabolism by the renal cortex and medulla
PROSTAGLANDINS
BIOSYNTHESISAND METABOLISM BY THE RENAL CORTEX AND MEDULLA
Sheng-ShungPong and Lawrence Levine Department of Biochemistry Brandeis University Waltham, Massachusetts02154
NAD+- and NADP+-dependent15-hydroxyprostaglandjn dehydrogenaseswere detected in the kidney of several species. Combined dehydrogenaseactivities were equally distributedbetween the renal cortical and medullary regions of monkey, dog, rat, and swine. The cortices of the rabbit, cat, and ox had greater dehydrogenaseactivity than the medullae. NADPH-dependentPGE 9-ketoreductase activity was present in the cytoplasmicfractions of renal cortex and medulla of these species. In addition, cytoplasmicNADH-dependent PGE 9-ketoreductaseactivity was detected in the kidney of rat and ox. A heat-labilenon-heme protein from the cytoplasm of rabbit renal medulla and hemoglobin stimulated the synthesis of PGE2 and PGF2, by rabbit renal medullary microsomes in the presence of arachidonicacid, hydroquinone,and reduced glutathione. Prostaglandinsynthetaseactivity was noted also in the renal cortex, lung, brain, spleen, uterus, and heart of the rabbit. Neither the protein in the medulla cytoplasmic fraction nor the hemoglobin stimulated the synthetic activity of microsomes from renal cortex, lung, brain, spleen, uterus, and heart. Indomethacin,flufenamicacid, and aspirin inhibited the production of prostaglandinsby the above microsomal preparations, and under identical-in vitro conditions,the inhibitionby each of the drugs was quantitativelysimilar for each tissue.
MARCH
1976
VOL. 11 NO. 3
477
BIOSYNTHESISAND METABOLISM BY THE RENAL CORTEX AND MEDULLA
Sheng-ShungPong and Lawrence Levine Department of Biochemistry Brandeis University Waltham, Massachusetts02154
NAD+- and NADP+-dependent15-hydroxyprostaglandjn dehydrogenaseswere detected in the kidney of several species. Combined dehydrogenaseactivities were equally distributedbetween the renal cortical and medullary regions of monkey, dog, rat, and swine. The cortices of the rabbit, cat, and ox had greater dehydrogenaseactivity than the medullae. NADPH-dependentPGE 9-ketoreductase activity was present in the cytoplasmicfractions of renal cortex and medulla of these species. In addition, cytoplasmicNADH-dependent PGE 9-ketoreductaseactivity was detected in the kidney of rat and ox. A heat-labilenon-heme protein from the cytoplasm of rabbit renal medulla and hemoglobin stimulated the synthesis of PGE2 and PGF2, by rabbit renal medullary microsomes in the presence of arachidonicacid, hydroquinone,and reduced glutathione. Prostaglandinsynthetaseactivity was noted also in the renal cortex, lung, brain, spleen, uterus, and heart of the rabbit. Neither the protein in the medulla cytoplasmic fraction nor the hemoglobin stimulated the synthetic activity of microsomes from renal cortex, lung, brain, spleen, uterus, and heart. Indomethacin,flufenamicacid, and aspirin inhibited the production of prostaglandinsby the above microsomal preparations, and under identical-in vitro conditions,the inhibitionby each of the drugs was quantitativelysimilar for each tissue.
MARCH
1976
VOL. 11 NO. 3
477