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6 mice fed with a high fat diet
Abstracts / Toxicology Letters 205S (2011) S60–S179
P1272 Transcriptional induction of P450 enzymes cyp1a1 and cyp1a2 after caffeine and chios mastic gum administration in male Wistar rats E. Katsanou 1,∗ , K. Kyriakopoulou 1 , M. Meintanis 1 , N. Fokialakis 2 , K. Machera 1 1
Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, Kifissia, Athens, Greece, 2 University of Athens, Athens, Greece Purpose: CYP1A1 and CYP1A2 enzymes belong to the superfamily of P450 enzymes and are among the most involved in the biotransformation of chemicals and the metabolic activation of precarcinogens. Thus, the induction of these enzymes is considered to be of particular toxicological significance for the human organism. In the present study the transcriptional activation of CYP1A1 and CYP1A2 enzymes in the liver of male Wistar rats, following oral administration of Chios Mastic Gum (CMG) at the maximum recommended pharmaceutical dose, was compared to the respective CYP activation following oral administration of caffeine. Methods: Four groups of rats, were dosed orally by gavage with 0, 30 and 100 mg/kg b.w./day of caffeine or with 2000 mg/kg b.w./day of CMG for 3 consecutive days. The transcription of CYP1A1 and CYP1A2 was measured by using the method of RT-qPCR and the relative quantification of mRNAs was calculated with the CT method. Results: From the obtained results it is evident that only the high dose of caffeine can induce rat hepatic CYP1A1 mRNA at toxicologically significant level (18-fold), while the low dose of caffeine and CMG caused only 1.5- and 3.5-fold induction of CYP1A1 mRNA, respectively. With respect to CYP1A2, a 2.6 fold induction of mRNA was observed after administration of high-dose caffeine, while no significant change was observed after administration of low-dose caffeine and CMG. These results indicate that administration of CMG at the recommended pharmaceutical dose does not induce transcriptional activation of CYP1A enzymes involved in metabolic activation of pre-carcinogens. doi:10.1016/j.toxlet.2011.05.506
P1273 Mercury in fish from the important fish location (Czech Republic) K. Kruzikova 1,∗ , J. Blahova 2 , R. Kensova 2 , R. Dobsikova 2 , Z. Svobodova 2 1
Public Veterinary Health and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic, 2 University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic The occurrence and concentration of mercury in water environment is a hot topic and attention is paid to hygienic aspects. From the food safety point of view, it is necessary to observe both low and high contaminated locations and to evaluate the hygienic quality and food safety of fish which can be included in food chain. From six important fishing locations were caught total of 142 predatory and non-predatory species fish in the spring 2010. Locations chosen (rivers: LuÅ3/4 nice, Berounka upper the junction with Vltava, river Otava (2 places); water reservoirs Jordán and Trnávka) belong to important fishing grounds and are much-frequented by sport fishermen. Mercury and methylmercury content in the different tissue (muscle, liver, gonads and scales) were analysed. Mercury assessment was performed using atomic
S143
absorption spectrometry method and AMA 254 analyser, speciation of methylmercury was performed using gas chromatography with ECD detection (GC2010A chromatograph). The highest values of THg were found in asp and reach up to 0.7 mg/kg (the Berounka river), however, for nonpredatory fish the mercury content did not exceed 0.5 mg/kg. The lowest THg content was found in carp (0.05 mg/kg). Mercury content decreased in different organs subsequently: muscle > liver > gonads > scales. Total mercury includes 55–100% of methylmercury. This research was supported by the project MSM 6215712402 and IGA 84/2010/FVHE. doi:10.1016/j.toxlet.2011.05.507
P1274 Bisphenol a exposure in C57Bl/6 mice fed with a high fat diet L. Le Corre 1,∗ , P. Besnard 2 , M.C. Chagnon 1 Derttech Packtox, AgroSupDijon, Dijon, France, 2 Nutrition Physiology Laboratory, AgrosupDijon - INSERM U866, Dijon, France
1
Purpose: Determine the impact of bisphenol A peri-natal exposure in the promotion of the obesity in a mice model. Methods: C57Bl/6 mice was exposed to 0.2, 20, 2000 g/kg bw/d BPA in drinking water. BPA treatment began at gestational day 6 and continued in offspring up to 36-weeks old. Four weeks after weaning, mice from treated-dams were fed with a high fat diet (60% kcal as fat). During the BPA exposure, body weight, fat body mass and energy expenditure were checked. These parameters were assessed using small animal physiology platform (Nutrition Physiology Laboratory, INSERM U866). Fat body mass was measured using ECHO-MRI systems (Echo Medical Systems, Houston, USA) and energy expenditure with CLAMS systems. Results: First results showed a sex-dependent effect of BPA exposure. Body weight of male pups born to BPA-exposed dams was significantly higher (15–30%) compared with controls. Only females born to dams exposed to the highest BPA dose exhibited a body weight gain. Interestingly, these observations appear in positive correlation with adipose tissue mass (Echo Medical Systems, Houston, USA) and consequently in negative correlation with O2 consumption and energy expenditure. Our data suggest that BPA low dose exposure in gestational and in a chronic manner potentiate weight gain and consequently obesity due to high fat diet. doi:10.1016/j.toxlet.2011.05.508
P1275 Saccharomyces cereviciae potentially inhibit ochratoxicosis in male albino mice S.A. Nada 1,∗ , H.A. Amra 2 , K.B. Abdel-Aziz 3 , E.A. Omara 4 , I.M. Farag 3 , N.S. Tawfek 5 , H.R. Darwish 3 Pharmacology, National Research Centre, Cairo, Egypt, 2 Food Toxicology and Contaminants, National Research Centre, Cairo, Egypt, 3 Cell Biology, National Research Centre, Cairo, Egypt, 4 Pathology, National Research Centre, Cairo, Egypt, 5 Zoology, Al-Mania University, Almania, Egypt
1
The present study was conducted to investigate the possible protective effect Saccharomyces cereviciae (S. cereviciae) against ochratoxin (OA)-induced toxicity in mice. Four groups of mice were treated for 7 successive days: control group given saline (10 ml/kg), group 2: administered S. cereviciae (1 × 108 CFU/kg), group3: orally
P1272 Transcriptional induction of P450 enzymes cyp1a1 and cyp1a2 after caffeine and chios mastic gum administration in male Wistar rats E. Katsanou 1,∗ , K. Kyriakopoulou 1 , M. Meintanis 1 , N. Fokialakis 2 , K. Machera 1 1
Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, Kifissia, Athens, Greece, 2 University of Athens, Athens, Greece Purpose: CYP1A1 and CYP1A2 enzymes belong to the superfamily of P450 enzymes and are among the most involved in the biotransformation of chemicals and the metabolic activation of precarcinogens. Thus, the induction of these enzymes is considered to be of particular toxicological significance for the human organism. In the present study the transcriptional activation of CYP1A1 and CYP1A2 enzymes in the liver of male Wistar rats, following oral administration of Chios Mastic Gum (CMG) at the maximum recommended pharmaceutical dose, was compared to the respective CYP activation following oral administration of caffeine. Methods: Four groups of rats, were dosed orally by gavage with 0, 30 and 100 mg/kg b.w./day of caffeine or with 2000 mg/kg b.w./day of CMG for 3 consecutive days. The transcription of CYP1A1 and CYP1A2 was measured by using the method of RT-qPCR and the relative quantification of mRNAs was calculated with the CT method. Results: From the obtained results it is evident that only the high dose of caffeine can induce rat hepatic CYP1A1 mRNA at toxicologically significant level (18-fold), while the low dose of caffeine and CMG caused only 1.5- and 3.5-fold induction of CYP1A1 mRNA, respectively. With respect to CYP1A2, a 2.6 fold induction of mRNA was observed after administration of high-dose caffeine, while no significant change was observed after administration of low-dose caffeine and CMG. These results indicate that administration of CMG at the recommended pharmaceutical dose does not induce transcriptional activation of CYP1A enzymes involved in metabolic activation of pre-carcinogens. doi:10.1016/j.toxlet.2011.05.506
P1273 Mercury in fish from the important fish location (Czech Republic) K. Kruzikova 1,∗ , J. Blahova 2 , R. Kensova 2 , R. Dobsikova 2 , Z. Svobodova 2 1
Public Veterinary Health and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic, 2 University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic The occurrence and concentration of mercury in water environment is a hot topic and attention is paid to hygienic aspects. From the food safety point of view, it is necessary to observe both low and high contaminated locations and to evaluate the hygienic quality and food safety of fish which can be included in food chain. From six important fishing locations were caught total of 142 predatory and non-predatory species fish in the spring 2010. Locations chosen (rivers: LuÅ3/4 nice, Berounka upper the junction with Vltava, river Otava (2 places); water reservoirs Jordán and Trnávka) belong to important fishing grounds and are much-frequented by sport fishermen. Mercury and methylmercury content in the different tissue (muscle, liver, gonads and scales) were analysed. Mercury assessment was performed using atomic
S143
absorption spectrometry method and AMA 254 analyser, speciation of methylmercury was performed using gas chromatography with ECD detection (GC2010A chromatograph). The highest values of THg were found in asp and reach up to 0.7 mg/kg (the Berounka river), however, for nonpredatory fish the mercury content did not exceed 0.5 mg/kg. The lowest THg content was found in carp (0.05 mg/kg). Mercury content decreased in different organs subsequently: muscle > liver > gonads > scales. Total mercury includes 55–100% of methylmercury. This research was supported by the project MSM 6215712402 and IGA 84/2010/FVHE. doi:10.1016/j.toxlet.2011.05.507
P1274 Bisphenol a exposure in C57Bl/6 mice fed with a high fat diet L. Le Corre 1,∗ , P. Besnard 2 , M.C. Chagnon 1 Derttech Packtox, AgroSupDijon, Dijon, France, 2 Nutrition Physiology Laboratory, AgrosupDijon - INSERM U866, Dijon, France
1
Purpose: Determine the impact of bisphenol A peri-natal exposure in the promotion of the obesity in a mice model. Methods: C57Bl/6 mice was exposed to 0.2, 20, 2000 g/kg bw/d BPA in drinking water. BPA treatment began at gestational day 6 and continued in offspring up to 36-weeks old. Four weeks after weaning, mice from treated-dams were fed with a high fat diet (60% kcal as fat). During the BPA exposure, body weight, fat body mass and energy expenditure were checked. These parameters were assessed using small animal physiology platform (Nutrition Physiology Laboratory, INSERM U866). Fat body mass was measured using ECHO-MRI systems (Echo Medical Systems, Houston, USA) and energy expenditure with CLAMS systems. Results: First results showed a sex-dependent effect of BPA exposure. Body weight of male pups born to BPA-exposed dams was significantly higher (15–30%) compared with controls. Only females born to dams exposed to the highest BPA dose exhibited a body weight gain. Interestingly, these observations appear in positive correlation with adipose tissue mass (Echo Medical Systems, Houston, USA) and consequently in negative correlation with O2 consumption and energy expenditure. Our data suggest that BPA low dose exposure in gestational and in a chronic manner potentiate weight gain and consequently obesity due to high fat diet. doi:10.1016/j.toxlet.2011.05.508
P1275 Saccharomyces cereviciae potentially inhibit ochratoxicosis in male albino mice S.A. Nada 1,∗ , H.A. Amra 2 , K.B. Abdel-Aziz 3 , E.A. Omara 4 , I.M. Farag 3 , N.S. Tawfek 5 , H.R. Darwish 3 Pharmacology, National Research Centre, Cairo, Egypt, 2 Food Toxicology and Contaminants, National Research Centre, Cairo, Egypt, 3 Cell Biology, National Research Centre, Cairo, Egypt, 4 Pathology, National Research Centre, Cairo, Egypt, 5 Zoology, Al-Mania University, Almania, Egypt
1
The present study was conducted to investigate the possible protective effect Saccharomyces cereviciae (S. cereviciae) against ochratoxin (OA)-induced toxicity in mice. Four groups of mice were treated for 7 successive days: control group given saline (10 ml/kg), group 2: administered S. cereviciae (1 × 108 CFU/kg), group3: orally