- Email: [email protected]
Blood cell changes during encapsulation reactions in Drosophila
148
IMMUNOENDOCRINOLOGY
Vol. i0, No. 1
BLOOD CELL CHANGES DURING ENCAPSULATION REACTIONS IN D_R_Q~Q~ILA. A. J. N a p p i and M. Silvers. Loyola Univ. of Chicago, Chicago, Illinois USA. The larval blood cells of Drosgp~il~ ~ I ~ I D ~ X function effectively in various inflammatory and immune responses. When the cellular immune system is stimulated, large numbers of spherical plasmatocytes differentiate into flat, disc-shaped lamellocytes, and both types of hemocyte bind together forming multilayered capsules around foreign surfaces. Crystal cells, a major source of hemolymph phenoloxidases, lyse and release substances that cause melanization of the capsules. Hemocyte surface changes during encapsulation reactions are identified by a FITCconjugated lectin. These studies distinguish subpopulations of hemocytes, some of which possess altered surface properties that may potentiate their adhesion. Hemocytes cultured with dopamine, a monoamine neurotransmitter substance with hormone-like activity, exhibit enhanced lectin binding similar to that observed in cellular encapsulation.
PRESENCE OF FACTEUR THYMIQUE SERIQUE (FTS) IN THYMIC EPITHELIAL CELLS OF DICENTRARCHUS LABRAX AND CYPRINUS CARPIO. P.A. Deschaux and M.F. P e r r e t - F r o e h l y . Univ. of Limoges, LIMOGES, France. In Mammals, d i f f e r e n t i a t i o n and m a t u r a t i o n of T lymphocytes are under the control of thymic hormones s e c r e t e d by thymic epithelial cells (TEC). Using indirect immunofluorescence we have d e m o n s t r a t e d the p r e s e n c e of one thymic h o r m o ne : f a c t e u r thymique serique (FTS), in two fishes : Dicentrarchus labrax (DL) and Cyprinus carpio (CC). In a first t i m e tonofilaments of TEC are c h a r a c t e r i z e d with an antikeratine antibody. In a second time we used an anti FTS monoclonal antibody (kindly p r e p a r e d by J.F. Bach and Coll.) ; this last reaction is more intense in TEC of DL. Into the carp the size of TEC is smaller, with woolly outlines. These last result can be related with a more important level of an other thymic hormone (thymosin f r a c t i o n 5) found in DL [P. Deschaux and al., IRCS Med. Sci., 12, 977978, 1984].
IMMUNOENDOCRINOLOGY
Vol. i0, No. 1
BLOOD CELL CHANGES DURING ENCAPSULATION REACTIONS IN D_R_Q~Q~ILA. A. J. N a p p i and M. Silvers. Loyola Univ. of Chicago, Chicago, Illinois USA. The larval blood cells of Drosgp~il~ ~ I ~ I D ~ X function effectively in various inflammatory and immune responses. When the cellular immune system is stimulated, large numbers of spherical plasmatocytes differentiate into flat, disc-shaped lamellocytes, and both types of hemocyte bind together forming multilayered capsules around foreign surfaces. Crystal cells, a major source of hemolymph phenoloxidases, lyse and release substances that cause melanization of the capsules. Hemocyte surface changes during encapsulation reactions are identified by a FITCconjugated lectin. These studies distinguish subpopulations of hemocytes, some of which possess altered surface properties that may potentiate their adhesion. Hemocytes cultured with dopamine, a monoamine neurotransmitter substance with hormone-like activity, exhibit enhanced lectin binding similar to that observed in cellular encapsulation.
PRESENCE OF FACTEUR THYMIQUE SERIQUE (FTS) IN THYMIC EPITHELIAL CELLS OF DICENTRARCHUS LABRAX AND CYPRINUS CARPIO. P.A. Deschaux and M.F. P e r r e t - F r o e h l y . Univ. of Limoges, LIMOGES, France. In Mammals, d i f f e r e n t i a t i o n and m a t u r a t i o n of T lymphocytes are under the control of thymic hormones s e c r e t e d by thymic epithelial cells (TEC). Using indirect immunofluorescence we have d e m o n s t r a t e d the p r e s e n c e of one thymic h o r m o ne : f a c t e u r thymique serique (FTS), in two fishes : Dicentrarchus labrax (DL) and Cyprinus carpio (CC). In a first t i m e tonofilaments of TEC are c h a r a c t e r i z e d with an antikeratine antibody. In a second time we used an anti FTS monoclonal antibody (kindly p r e p a r e d by J.F. Bach and Coll.) ; this last reaction is more intense in TEC of DL. Into the carp the size of TEC is smaller, with woolly outlines. These last result can be related with a more important level of an other thymic hormone (thymosin f r a c t i o n 5) found in DL [P. Deschaux and al., IRCS Med. Sci., 12, 977978, 1984].