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Blood lipid spectrum in patients with type 2 diabetes depending on polymorphism of irs-1 gene
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Abstracts / Atherosclerosis 241 (2015) e149ee229
EAS-0898. CORONARY ARTERY DISEASE DETECTED BY CORONARY COMPUTED TOMOGRAPHY ANGIOGRAPHY IS ASSOCIATED WITH RED CELL DISTRIBUTION WIDTH E. Karacaglar a, U. Bal a, S. Hasirci a, M. Yilmaz a, E. Doganozu a, M. Coskun b, I. Atar a, H. Muderrisoglu a. a Cardiology, Baskent University, Ankara, Turkey; b Radiology, Baskent University, Ankara, Turkey Background: Increased red blood cell width (RDW) is associated with presence and severity of coronary artery disease (CAD). Objective: The aim of the present study was to evaluate the relation between CAD detected by coronary computed tomography angiography (CCTA) and RDW retrospectively. Methods: We evaluated medical records of 316 patients who underwent 16-slice CCTA due to the presence of angina-like chest pain, retrospectively. Patients without exclusion criteria were divided into two groups based on the results of CCTA. Clinical characteristics, risk factors for CAD and RDW values at the time of CCTA were noted. Results: The RDW levels in patients with CAD were significantly higher compared with NCA group (15.50±1.57 to 14.80±1.41, p¼0.001). Diabetes mellitus, hypertension and smoking history were significantly more common in CAD group (p¼0.018; p¼0.007; p¼0.013, respectively). In multivariate Logistic regression analysis, RDW (OR¼1.35, 95% CI: 1.08-1.68, P ¼0.009), age (OR¼1.06, 95% CI: 1.03-1.09, P<0.001) and smoking history (OR¼2.66, 95% CI: 1.36-5.23, P¼0.003) were demonstrated to be an independent predictor for CAD detected by CCTA. Conclusion: Our results suggest that higher RDW levels are independently associated with presence of coronary artery disease detected by CCTA in patients without known CAD. Further studies are needed to clarify role of RDW in risk stratification.
Table Predictors of CAD detected by CCTA in multivariate logistic regression analysis. Variable
OR
95% CI
P values
Age Smoking history Diabetes Mellitus Hypertension Hyperlipidemia RDW
1.06 2.67 1.74 1.26 1.45 1.35
1.03-1.09 1.36-5.23 0.68-4.45 0.65-2.43 0.77-2.72 1.08-1.68
<0.001 0.003 0.533 0.944 0.943 0.009
EAS-0901. N-STEAROYLETHANOLAMINE IMPROVES INDICATORS OF INSULIN RESISTANCE DEVELOPMENT UNDER HIGH-FAT DIET OVERLOAD O.V. Onopchenko, G.V. Kosiakova, N.M. Gula. Biochemistry of lipids, Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU), Kiev, Ukraine Obesity is the major problem of modern society, which is associated with such metabolic disorders as insulin resistance (IR) and type II diabetes. Thus, it is of a current interest to develop novel treatment modalities that could improve the lipid imbalance and restore insulin sensitivity. Recent studies indicate that bioactive lipid N-stearoylethanolamine is able to correct lipid imbalance, showing membrane-stabilizing and cytoprotective properties. According to this, we explore the effect of NSE on indicators of IR development at a rat model of HFD overload. The existence of IR was estimated by results of oral glucose tolerance test, after that animals were divided into 4 groups: «Control», «NSE», «IR», «IR+NSE»; first two groups were fed with regular chow (4% fat), and other two groups were given HFD (58% fat) for 6 months. At the end of the experiment «NSE», «IR+NSE» rats were given per os water suspension of NSE (50 mg/kg daily) for 2 weeks. After the HFD overload, rats that were treated by NSE showed improved glucose tolerance (the level of blood glucose within 150 min after oral
glucose administration was 4.4 mmol/L). Moreover, the plasma level of insulin was 20% lower than those of IR-group and was accompanied by a reduction in HOMA-IR value (56% lower than in IR rats). In 'IR+NSE' group we found a slight decrease (15%) in glycated hemoglobin level compared to 'IR' group, where it was 33% higher than in controls. Therefore, the NSE administration to IR rats resulted in improvement of insulin sensitivity.
EAS-0908. BLOOD LIPID SPECTRUM IN PATIENTS WITH TYPE 2 DIABETES DEPENDING ON POLYMORPHISM OF IRS-1 GENE A. Shalimova a, A. Belovol b, M. Kochueva a. a Therapy and Nephrology, Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine; b Clinical Pharmacology, Kharkiv National Medical University, Kharkiv, Ukraine Recent years, it has been believed that insulin resistance (IR) is the independent risk factor of dyslipidemia, systemic inflammation, oxidative stress. Nowadays in different sources we can find quite contradictory facts about the role of polymorphism insulin receptor substrate-1 (IRS-1) in the development of IR and its consequences. The aim of the study was to investigate the gene polymorphism IRS-1 and its associations with indicators of blood lipid spectrum in patients with type 2 diabetes (DM2) and essential hypertension (EH). The main group of our study consisted of 94 patients with DM2 in combination with EH. The group of comparison consisted of 20 healthy subjects. Determined lipid and carbohydrate profiles, IR assessed by HOMA index, conducted genotyping of polymorphic marker rs1801278 gene IRS-1. According to the results of genotyping Gly972Arg in 10.64% patients with DM2 was identified homozygous polymorphism and in 41.49% patients were considered heterozygous polymorphism. Healthy patients had only heterozygous polymorphisms (in 10 % of cases). It was established that patients with DM2 and homozygous polymorphism (Arg/Arg) have higher levels of total cholesterol, triglycerides, low-density lipoproteins, HOMA index that patients with other genotypes. Conclusion: It was established the association of the homozygous polymorphism of polymorphic marker rs1801278 gene IRS-1 with the development of DM2 in Ukrainian population. In patients with DM2 genotype Arg/Arg combined with elevated levels of atherogenic lipoproteins and more pronounced IR.
EAS-0565. ACUTE HEART FAILURE IN PATIENTS WITH PERIPHERAL VASCULAR DISEASE: INSIGHTS FROM THE FIRST GULF ACUTE HEART FAILURE REGISTRY (GULF CARE) H. Al-Thani a, A. El-Menyar b, K. Sulaiman c, N. Asaad d, J. Al-Suwaidi d, A. Alsheikh-Ali e, K. Al-Habib f, W. Al Mahmeed e, A. Al-Motarreb g, H. Amin h, R. Singh d, M. Ridha i. a Vascular Surgery, Hamad General Hospital, Doha, Qatar; b Clinicall Medicine, Hamad General Hospital, Doha, Qatar; c Cardiology, Royal Hospital, Muscat, Oman; d Cardiology, Hamad General Hospital, Doha, Qatar; e Cardiology, Sheikh Khalifa Medical City, Abu Dhabi, United Arab Emirates; f Cardiology, King Fahad Cardiac Center, Riyadh, Saudi Arabia; g Cardiology, Sanaa University, Sanaa, Yemen; h Cardiology, Mohammed bib Khalifa, Manama, Bahrain; i Cardiology, Adnan Hospital, Kuwait, Kuwait Aim: to study the frequency and impact of peripheral vascular disease in patients presenting with acute heart failure (AHF). Methods: Gulf CARE is a prospective, multicenter, multinational registry of adult patients admitted with AHF with a 3 months and 1-year follow-up. We reanalyzed data and categorized patients into group1(without PVD) and group2 (with PVD). Results: A total of 5005 consecutive AHF patients were enrolled in 2012. Forty-seven hospitals in 7 Gulf States. PVD constituted 4.5% (n¼223) of all cases admitted with AHF with a mean age of 60±15 years (p¼0.61). In
Abstracts / Atherosclerosis 241 (2015) e149ee229
EAS-0898. CORONARY ARTERY DISEASE DETECTED BY CORONARY COMPUTED TOMOGRAPHY ANGIOGRAPHY IS ASSOCIATED WITH RED CELL DISTRIBUTION WIDTH E. Karacaglar a, U. Bal a, S. Hasirci a, M. Yilmaz a, E. Doganozu a, M. Coskun b, I. Atar a, H. Muderrisoglu a. a Cardiology, Baskent University, Ankara, Turkey; b Radiology, Baskent University, Ankara, Turkey Background: Increased red blood cell width (RDW) is associated with presence and severity of coronary artery disease (CAD). Objective: The aim of the present study was to evaluate the relation between CAD detected by coronary computed tomography angiography (CCTA) and RDW retrospectively. Methods: We evaluated medical records of 316 patients who underwent 16-slice CCTA due to the presence of angina-like chest pain, retrospectively. Patients without exclusion criteria were divided into two groups based on the results of CCTA. Clinical characteristics, risk factors for CAD and RDW values at the time of CCTA were noted. Results: The RDW levels in patients with CAD were significantly higher compared with NCA group (15.50±1.57 to 14.80±1.41, p¼0.001). Diabetes mellitus, hypertension and smoking history were significantly more common in CAD group (p¼0.018; p¼0.007; p¼0.013, respectively). In multivariate Logistic regression analysis, RDW (OR¼1.35, 95% CI: 1.08-1.68, P ¼0.009), age (OR¼1.06, 95% CI: 1.03-1.09, P<0.001) and smoking history (OR¼2.66, 95% CI: 1.36-5.23, P¼0.003) were demonstrated to be an independent predictor for CAD detected by CCTA. Conclusion: Our results suggest that higher RDW levels are independently associated with presence of coronary artery disease detected by CCTA in patients without known CAD. Further studies are needed to clarify role of RDW in risk stratification.
Table Predictors of CAD detected by CCTA in multivariate logistic regression analysis. Variable
OR
95% CI
P values
Age Smoking history Diabetes Mellitus Hypertension Hyperlipidemia RDW
1.06 2.67 1.74 1.26 1.45 1.35
1.03-1.09 1.36-5.23 0.68-4.45 0.65-2.43 0.77-2.72 1.08-1.68
<0.001 0.003 0.533 0.944 0.943 0.009
EAS-0901. N-STEAROYLETHANOLAMINE IMPROVES INDICATORS OF INSULIN RESISTANCE DEVELOPMENT UNDER HIGH-FAT DIET OVERLOAD O.V. Onopchenko, G.V. Kosiakova, N.M. Gula. Biochemistry of lipids, Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU), Kiev, Ukraine Obesity is the major problem of modern society, which is associated with such metabolic disorders as insulin resistance (IR) and type II diabetes. Thus, it is of a current interest to develop novel treatment modalities that could improve the lipid imbalance and restore insulin sensitivity. Recent studies indicate that bioactive lipid N-stearoylethanolamine is able to correct lipid imbalance, showing membrane-stabilizing and cytoprotective properties. According to this, we explore the effect of NSE on indicators of IR development at a rat model of HFD overload. The existence of IR was estimated by results of oral glucose tolerance test, after that animals were divided into 4 groups: «Control», «NSE», «IR», «IR+NSE»; first two groups were fed with regular chow (4% fat), and other two groups were given HFD (58% fat) for 6 months. At the end of the experiment «NSE», «IR+NSE» rats were given per os water suspension of NSE (50 mg/kg daily) for 2 weeks. After the HFD overload, rats that were treated by NSE showed improved glucose tolerance (the level of blood glucose within 150 min after oral
glucose administration was 4.4 mmol/L). Moreover, the plasma level of insulin was 20% lower than those of IR-group and was accompanied by a reduction in HOMA-IR value (56% lower than in IR rats). In 'IR+NSE' group we found a slight decrease (15%) in glycated hemoglobin level compared to 'IR' group, where it was 33% higher than in controls. Therefore, the NSE administration to IR rats resulted in improvement of insulin sensitivity.
EAS-0908. BLOOD LIPID SPECTRUM IN PATIENTS WITH TYPE 2 DIABETES DEPENDING ON POLYMORPHISM OF IRS-1 GENE A. Shalimova a, A. Belovol b, M. Kochueva a. a Therapy and Nephrology, Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine; b Clinical Pharmacology, Kharkiv National Medical University, Kharkiv, Ukraine Recent years, it has been believed that insulin resistance (IR) is the independent risk factor of dyslipidemia, systemic inflammation, oxidative stress. Nowadays in different sources we can find quite contradictory facts about the role of polymorphism insulin receptor substrate-1 (IRS-1) in the development of IR and its consequences. The aim of the study was to investigate the gene polymorphism IRS-1 and its associations with indicators of blood lipid spectrum in patients with type 2 diabetes (DM2) and essential hypertension (EH). The main group of our study consisted of 94 patients with DM2 in combination with EH. The group of comparison consisted of 20 healthy subjects. Determined lipid and carbohydrate profiles, IR assessed by HOMA index, conducted genotyping of polymorphic marker rs1801278 gene IRS-1. According to the results of genotyping Gly972Arg in 10.64% patients with DM2 was identified homozygous polymorphism and in 41.49% patients were considered heterozygous polymorphism. Healthy patients had only heterozygous polymorphisms (in 10 % of cases). It was established that patients with DM2 and homozygous polymorphism (Arg/Arg) have higher levels of total cholesterol, triglycerides, low-density lipoproteins, HOMA index that patients with other genotypes. Conclusion: It was established the association of the homozygous polymorphism of polymorphic marker rs1801278 gene IRS-1 with the development of DM2 in Ukrainian population. In patients with DM2 genotype Arg/Arg combined with elevated levels of atherogenic lipoproteins and more pronounced IR.
EAS-0565. ACUTE HEART FAILURE IN PATIENTS WITH PERIPHERAL VASCULAR DISEASE: INSIGHTS FROM THE FIRST GULF ACUTE HEART FAILURE REGISTRY (GULF CARE) H. Al-Thani a, A. El-Menyar b, K. Sulaiman c, N. Asaad d, J. Al-Suwaidi d, A. Alsheikh-Ali e, K. Al-Habib f, W. Al Mahmeed e, A. Al-Motarreb g, H. Amin h, R. Singh d, M. Ridha i. a Vascular Surgery, Hamad General Hospital, Doha, Qatar; b Clinicall Medicine, Hamad General Hospital, Doha, Qatar; c Cardiology, Royal Hospital, Muscat, Oman; d Cardiology, Hamad General Hospital, Doha, Qatar; e Cardiology, Sheikh Khalifa Medical City, Abu Dhabi, United Arab Emirates; f Cardiology, King Fahad Cardiac Center, Riyadh, Saudi Arabia; g Cardiology, Sanaa University, Sanaa, Yemen; h Cardiology, Mohammed bib Khalifa, Manama, Bahrain; i Cardiology, Adnan Hospital, Kuwait, Kuwait Aim: to study the frequency and impact of peripheral vascular disease in patients presenting with acute heart failure (AHF). Methods: Gulf CARE is a prospective, multicenter, multinational registry of adult patients admitted with AHF with a 3 months and 1-year follow-up. We reanalyzed data and categorized patients into group1(without PVD) and group2 (with PVD). Results: A total of 5005 consecutive AHF patients were enrolled in 2012. Forty-seven hospitals in 7 Gulf States. PVD constituted 4.5% (n¼223) of all cases admitted with AHF with a mean age of 60±15 years (p¼0.61). In