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Body energy metabolism and oxidative stress in mice supplemented with conjugated linoleic acid (CLA) associated to oleic acid
Free Radical Biology and Medicine 75 (2014) S21–S53
Contents lists available at ScienceDirect
Free Radical Biology and Medicine journal homepage: www.elsevier.com/locate/freeradbiomed
POSTERS
P1
Body energy metabolism and oxidative stress in mice supplemented with conjugated linoleic acid (CLA) associated to oleic acid
associated to oleic acid may be regarded as a potential strategy for controlling obesity and oxidative stress. Supported by FAPESP.
http://dx.doi.org/10.1016/j.freeradbiomed.2014.10.733
Baraldi Flavia, Dalalio Felipe, Teodoro Bruno, Prado Ieda, Curti Carlos, Alberici Luciane P2 Universidade de São Paulo (Faculdade de Ciências Farmacêuticas de Ribeirão Preto), Departamento de Física e Química, Brazil
In vitro glycation and glycoxidation of phosphatidylethanolamines
Abstract
Annibal Andreaa, Fedorova Mariab, Schiller Jürgenc, Hoffmann Ralfa
Introduction: Some fatty acids may play an important role in regulating metabolism through PPARs activation. Conjugated linoleic acid (CLA) has been shown to reduce body fat accumulation and increase body metabolism; this effect has been associated with up-regulation of mitochondrial uncoupling proteins (UCPs) and PPARalfa activation. Oleic acid has shown beneficial effects on health, decreasing oxidative stress and improving clinical conditions related to obesity. Therefore, in this work, we addressed the effects of a oleic plus CLA-supplemented murine diet on body metabolism, mitochondrial energetics and oxidative stress in the liver, as well as on other associated morphological and functional parameters in C57BL/6 mice.
a
Institute of Bioanalytical Chemistry (Center for Biotechnology and Biomedicine, LIFE-Leipzig Research Center for Civilization Diseases), Faculty of Chemistry and Mineralogy, University Leipzig, Germany b Institute of Bioanalytical Chemistry (Center for Biotechnology and Biomedicine), Faculty of Chemistry and Mineralogy, University Leipzig,, Germany c Institute for Medical Physics and Biophysics (Center for Biotechnology and Biomedicine), Medicine Faculty, University of Leipzig, Germany
Method:
The diet was supplemented with 2% CLA mixture (cis-9, trans-10 and trans-10, cis-12 isomers; 45% of each isomer) and/or 0.7% olive oil on alternating days (60 days) by gavage.
Results: The results showed that diet supplementation with CLA increases body metabolism and reduces lipid accumulation in adipose tissues. Groups that received oleic acid (oleic and CLA oleic) showed decreased levels of total cholesterol and cholesterol non-HDL, and increased levels of HDL-cholesterol. Livers of mice fed a diet supplemented with CLA showed high levels UCP2 mRNA, and the isolated hepatic mitochondria showed indications of UCP activity and increased ROS generation. Oleic acid partially reversed the lower lipid accumulation increasing PPARgamma content, reversed the higher ROS generation by liver mitochondria and improved liver oxidative status. Conclusions:
These results indicate a beneficial and secure dose of CLA and oleic acid for diet supplementation in mice, which increases body metabolism inducing UCP2 overexpression/activity in liver while preserving the redox state of the liver. Therefore, diet supplementation with CLA
0891-5849/& 2014 Published by Elsevier Inc.
Abstract Formation and accumulation of advanced glycation end products (AGEs) appear to correlate with many human diseases, such as atherosclerosis and inflammation. Whereas AGE-modified proteins relatively well studied, aminophospholipid AGE-adducts have been less intensively investigated. At elevated concentrations, glucose can react with amino groups of phosphatidylethanolamines (PE) to form Schiff bases or Amadori products, which can be further converted, under oxidative conditions, to various AGEs. Many of these products such as carboxymethylamine (CMA) and carboxyethylamine (CEA) can be formed by oxidative degradation of Amadori-products. The aim of this work was to investigate the different glycation and glycoxidation PE-adducts and to elucidate the most prominent mechanisms of AGE-PE formation. Four different aminophospholipids,[dipalmitoyl-(DPPE), palmitoyl-oleoyl(POPE), palmitoyl-linoleoyl-(PLPE) or palmitoyl-arachidonoyl-phosphatidylethanolamine(PAPE); all 1 mmol/L] were glycated in vitro (5 mmol/L glucose) and oxidized by the Fenton reaction (80 mmol/L FeSO4, 50 mmol/L H2O2) or
Contents lists available at ScienceDirect
Free Radical Biology and Medicine journal homepage: www.elsevier.com/locate/freeradbiomed
POSTERS
P1
Body energy metabolism and oxidative stress in mice supplemented with conjugated linoleic acid (CLA) associated to oleic acid
associated to oleic acid may be regarded as a potential strategy for controlling obesity and oxidative stress. Supported by FAPESP.
http://dx.doi.org/10.1016/j.freeradbiomed.2014.10.733
Baraldi Flavia, Dalalio Felipe, Teodoro Bruno, Prado Ieda, Curti Carlos, Alberici Luciane P2 Universidade de São Paulo (Faculdade de Ciências Farmacêuticas de Ribeirão Preto), Departamento de Física e Química, Brazil
In vitro glycation and glycoxidation of phosphatidylethanolamines
Abstract
Annibal Andreaa, Fedorova Mariab, Schiller Jürgenc, Hoffmann Ralfa
Introduction: Some fatty acids may play an important role in regulating metabolism through PPARs activation. Conjugated linoleic acid (CLA) has been shown to reduce body fat accumulation and increase body metabolism; this effect has been associated with up-regulation of mitochondrial uncoupling proteins (UCPs) and PPARalfa activation. Oleic acid has shown beneficial effects on health, decreasing oxidative stress and improving clinical conditions related to obesity. Therefore, in this work, we addressed the effects of a oleic plus CLA-supplemented murine diet on body metabolism, mitochondrial energetics and oxidative stress in the liver, as well as on other associated morphological and functional parameters in C57BL/6 mice.
a
Institute of Bioanalytical Chemistry (Center for Biotechnology and Biomedicine, LIFE-Leipzig Research Center for Civilization Diseases), Faculty of Chemistry and Mineralogy, University Leipzig, Germany b Institute of Bioanalytical Chemistry (Center for Biotechnology and Biomedicine), Faculty of Chemistry and Mineralogy, University Leipzig,, Germany c Institute for Medical Physics and Biophysics (Center for Biotechnology and Biomedicine), Medicine Faculty, University of Leipzig, Germany
Method:
The diet was supplemented with 2% CLA mixture (cis-9, trans-10 and trans-10, cis-12 isomers; 45% of each isomer) and/or 0.7% olive oil on alternating days (60 days) by gavage.
Results: The results showed that diet supplementation with CLA increases body metabolism and reduces lipid accumulation in adipose tissues. Groups that received oleic acid (oleic and CLA oleic) showed decreased levels of total cholesterol and cholesterol non-HDL, and increased levels of HDL-cholesterol. Livers of mice fed a diet supplemented with CLA showed high levels UCP2 mRNA, and the isolated hepatic mitochondria showed indications of UCP activity and increased ROS generation. Oleic acid partially reversed the lower lipid accumulation increasing PPARgamma content, reversed the higher ROS generation by liver mitochondria and improved liver oxidative status. Conclusions:
These results indicate a beneficial and secure dose of CLA and oleic acid for diet supplementation in mice, which increases body metabolism inducing UCP2 overexpression/activity in liver while preserving the redox state of the liver. Therefore, diet supplementation with CLA
0891-5849/& 2014 Published by Elsevier Inc.
Abstract Formation and accumulation of advanced glycation end products (AGEs) appear to correlate with many human diseases, such as atherosclerosis and inflammation. Whereas AGE-modified proteins relatively well studied, aminophospholipid AGE-adducts have been less intensively investigated. At elevated concentrations, glucose can react with amino groups of phosphatidylethanolamines (PE) to form Schiff bases or Amadori products, which can be further converted, under oxidative conditions, to various AGEs. Many of these products such as carboxymethylamine (CMA) and carboxyethylamine (CEA) can be formed by oxidative degradation of Amadori-products. The aim of this work was to investigate the different glycation and glycoxidation PE-adducts and to elucidate the most prominent mechanisms of AGE-PE formation. Four different aminophospholipids,[dipalmitoyl-(DPPE), palmitoyl-oleoyl(POPE), palmitoyl-linoleoyl-(PLPE) or palmitoyl-arachidonoyl-phosphatidylethanolamine(PAPE); all 1 mmol/L] were glycated in vitro (5 mmol/L glucose) and oxidized by the Fenton reaction (80 mmol/L FeSO4, 50 mmol/L H2O2) or