Body weight is related to the degree of endothelial activation in young healthy adults

Posters 19. Thrombosis and Haemostasis

132

2nd h* 2.34-1.1%, 4 th h 4.34-3.1%*, 6th h 9.04-2.3%, p < .05). After Arg treatment FMV decreased at the 2nd h, normalizing at the 4 th and 6th h (baseline 9.24-1.8%, 2nd h* 4.54-3.5%, 4 th h 8.34-3.4%, 6th h 9.9 4- 2.8%,* p < .05). Triglycerides increased at the 2nd and 4th h and decreased at the 6 th both durin;g the first test (baseline 634-10 mg/dl, 2nd h* 1154-27, 4 th h* 1364-64, C n h 924-47;* p < .05) and after Arg (baseline 514-12 mg/dl, 2nd h* 1024-40, 4th h* 1244- 74, 6th h 1064-77;* p < .05). No significant changes were observed in the other parameters. Conclusions: Postprandial endothelial impairment is only partly reduced by Arg administration. Moreover, this evidence suggests the importance of dietary choice for atherosclerosis prevention even in young healthy subjects.

~ - 3 - ~ BODY WEIGHT IS RELATED TO THE DEGREE OF ENDOTHELIAL ACTIVATION IN YOUNG HEALTHY ADULTS G. Makris 1, I.K. Karabinos 1, S.N. Koulouris 1, T. Theodoridis2, M. E1-Ali2, J. Lekatsas 1, A. Kranidis 1, T. Giannoulis 1, T. Karahalios 1, V. Kokkinou2, N. Exadaktylos 1. 11st Cardiology Dept.; 2Haematology Lab., Euangelismos

Hospital, Athens, Greece

(PTT) decreased in all groups: In N from 38.29+1.07 to 31.93-4-2.40 sec (p < 0.05), in HYP from 38.254-0.50 to 33.254-3.59 sec (p < 0.05) and in IHD from 38.634-0.50 to 34.444-3.48 sec, indicating increased activation of the coagulation cascade. TXB2 levels increased in both groups, in N from 99.14-68.2 to 204.24-122.3 pg/ml (p < 0.01), in HYP from 102.44-57.2 to 224.5+152.1 pg/ml (p < 0.01) and in IHD from 102.14-74.4 to 293.7 + 358.6 pg/ml (p < 0.01). 6-keto-PGFla levels increased in N from 962.44-533.9 to 11724-571.2 pg/ml (10 < 0.05), in HYP from 987.54-454.6 to 12034-413.2 pg/ml and in IHD from 815.34- 455.4 to 1078.34-373.9 pg/ml (p < 0.05). Platelet aggregation rate decreased with ADP by 37% (p < 0.01) in N, by 11% (p < 0.05) in HYP and by 8% (p < 0.05) in IHD while with collagen by 36% (p < 0.01) in N, by 9% (p < 0.05) in HYP and by 6% in IHD. Despite SNS activation expressed by increased levels of plasma catecholamines, pro-thrombotic changes (increased hematocrit and platelets and reduced PPT) and platelet activation (increased levels of TXB2) reduced platelet aggregation was observed in all groups. Endothelium release of prostacyclin contributes to the inhibition of platelet aggregation. Reduced inhibition of platelet aggregation during stress in patients with HYP and IHD may contribute to the increased incidence of atherosclerotic and thrombotic complications.

Purpose: Obesity is considered a risk factor for atherosclerosis while activation of vascular endothelium represents a crucial step in the development of the disease. The relationship between obesity and endothelial activation has not so far been elucidated. The aim of our study was to correlate serum levels of endothelial activation markers with weight (W), height (H) and body mass index (BMI) in young healthy adults. Methods: Study population consisted of 82 healthy subjects (hs) aged 234-4 years. We measured the W (kgr), and the H (m) ofhs and we calculated their BMI (kgr/m2). All hs had blood sampling to assess serum levels of the following endothelial activation markers: von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and thrombomoduline (THR). vWF was assessed with the method of Ristosetin Cofactor Activity (% activity in serum), while THR and t-PA were assessed with ELISA (ng/ml). Results: Statistical univariate analysis revealed the following: Thr

t-PA

VWF

W

R = 0.04 p = 0.69

R = 0.39 p = 0.0001

R = 0.06 p = 0.57

H

R = 0.06 p = 0.5

R = ~).05

p = 0.6

R = 0.17 p = 0.89

BMI

R = 0.65 p = 0.59

R =-0.01

p = 0.11

R = 0.03 p = 0.75

Conclusions: t-PA is the only endothelial activation marker strongly related to body weight in young hs. This finding may suggest that body weight plays an important role in endothelial activation. Furthermore, an additional benefit of weigh loss may include the improvement of endothelial performance.

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SYMPATHETIC NERVOUS SYSTEM (SNS) ACTIVATION

AND PLATELET FUNCTION IN NORMOTENSIVES, HYPERTENSIVES (HYP) AND PATIENTS WITH ISCHEMIC HEART DISEASE (IHD)

K. Petidis, S. Douma, M. Doumas, K. Vogiatzis, A. Kontopoulos, C. Zamboulis. 2nd Propedeutic. Department of Medicine, Dir Prof. M. PapadimittT"ou, Aristotelion University, Thessaloniki, Greece SNS activation is considered a trigger factor for acute ischemic events. Catecholamines (especially adrenaline) activate platelets and promote their aggregation. Stress induced platelett activation may be a link between SNS activation and acute ischemic events. Intact vascular endothelium is closely related to platelet function through the production of PGI2 and NO. The effect of physical stress on sympathetic activation and platelet aggregation was examined in 18 healthy subjects (N), 26 HYP pts and 21 IHD pts who were submitted in an exercise test. Plasma levels of catecholamines (NA, ADR), 6-keto-PGFla, Thromboxane B2 and platelet aggregation (nephelometry) were determined at rest and during exercise. Hematocrit and platelet number increased during exercise in all groups. Plasma levels of NA increased in N from 191.24-123.8 to 1332.44-890.1 pg/ml (p < 0.01), in HYP from 284.44-68.2 to 2357.34- 767.9 pg/ml (p < 0.01) and in IHD from 2344-65.3 to 1903.94-858.9pg/ml (p < 0.01). ADR levels increased in N from 87.94-50.7 to 201.34-138.2 pg/ml (p < 0.01), in HYP from 103.54-48.8 to 476.54-185.1 pg/ml (p < 0.01) and in IHD from 87.74-41.8 to 3604-179.4 pg/ml (p < 0.01). Partial Thromboplasine Time

~-~-i] DEPRESSION IS ASSOCIATED WITH AN IMPAIRED FIBRINOLYTIC CAPACITY IN CORONARY PATIENTS S.M. Consoli 1, K. Lahlou-Lafor~t, M. Alhenc-Gelas, M. Pomin, S. Bydlowski, E. Seigneur, A. Benetos, J.M. Kierzin, RY. Scarabin, E. Pert'in, R Ducimeti~re, M. Aiach, L. Guize. 1Department of C-L

Psychiatry, European Georges Pompidou Hospital, Paris, France An impaired fibrinolytic capacity is one of the potential mechanisms proposed to explain the role of vital exhaustion (VE) in the course of cardiovascular heart disease (CHD). A preliminary study on a small sample of healthy volunteers (W. Kop et al.) had found higher scores of plasminogen activator inhibitor (PAl-l) in exhausted individuals. The present study was aimed at exploring the association between VE and/or depression with PAIl in healthy volunteers and in CHD patients. Population consisted of two samples: sample 1 (healthy volunteers) composed of 123 males, aged 40 to 65, free from coronary heart disease and any medication, and sample 2 (CHD patients) composed of 108 male inpatients, 40 to 65, with documented CHD, admitted for angiography. Psychometric measures consisted of Maastricht Questionnaire (MQ) for VE and Centre for Epidemiological StudiesDepression scale (CES-D). Fribrinolytic measures included fibrinogen, PAl-1 activity and tissue plasminogen activator (tPA). Control variables were: blood pressure, smoking status, body mass index (BMI), glyceamia, triglycerids and cholesterol. 10% and 7% of healthy volunteers and respectively 47% and 37% of CHD patients could be considered as exhausted or depressed, using classical cut-off points (MQ /> 8; CES-D /> 17). CHD patients presented higher scores of BMI, glyceamia, cholesterol, but also of PAI-1, fibrinogen, tPA, and 50% of them suffered from hypertension. In sample 1, PAI-1 levels were associated neither with VE nor with depression, as well as considering the psychometric measures as continuous or categorical. Results did not change after controlling for physical confounding variables. In sample 2, PAI-1 activity was significantly correlated with continuous VE (r = 0.19; p = 0.04) and a trend was found towards higher PAI-1 levels in depressed patients (p = 0.06). PAI-1 levels were not associated with the extent of the coronary lesions computed after angiography, but with physical variables (positively with BMI, triglycerides and hypertension and negatively with age). After controlling for the latter in a multivariate analysis, PAI-1 levels were significantly higher in depressed CHD patients (p = 0.02). These results enlighten a new possible pathway explaining the role of depression as a cardiovascular risk factor. ~-~

INCREASED LEVELS OF PLASMA GLYCOPROTEIN V IN PERIPHERAL AND CORONARY ARTERY DISEASE

A.D. Blaun I , E Lanza2, P. Galajda 1, S. Moog2, J.P. Cazenave2, G.Y.H. Lip 1. 1Haemostasis, Thrombosis and Vascular Biology Unit,

Department of Medicine, City Hospital, Birmingham B18 7QH, UK; 21NSERM U.311, Etablissement Francais du San-Alsace, Strasbourg, France As platelet hyperactivity is important in atherosclerosis, we hypothesised higher levels ofplatelet membrane glycoprotein V (GPV) in patients with peripheral artery disease (PAD) and coronary artery disease (CAD) compared to controls. We also compared GPV with levels of another platelet membrane

72nd EAS Congress