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Brain derived neurotrophic factor expression in the serum and cerebrospinal fluid of patients with hydrocephalus
Abstracts
Introduction: The oxidative stress and inflammation are cooperative events involved in atherosclerosis development. In the present study we assessed the association of MDA, antioxidant markers, high sensitive C -reactive protein (hs-CRP) and lipid status parameters in the patients with coronary artery disease (CAD). Significant risk factors such as cigarette and diabet were excluded from the study. Materials and Methods: Oxidative stress parameters for example Malondialdehyde (MDA), antioxidant markers including: erythrocyte superoxide dismutase (SOD), Glutathion peroxidase (GPX), total antioxidant capacity (TAC), the inflammation marker and serum lipid status parameters were measured in 120 subjects including 60 CAD patients with angiographically diagnosed CAD and 60 CAD-free subjects as a control group, also diabetics, smoking patients, patients with malignancy, renal and liver disease, and other disease were excluded from the study. Results: The serum MDA and hs-CRP levels were increased significantly as compared to controls. However, erythrocyte SOD, GPX activities and TAC level were reduced significantly in patients (P<0.05 in all cases). The levels of total cholesterol, Triglyceride, LDL-C were significantly higher and that of HDL-C was meaningfully lower than those of control (P<0.05 in all cases). Conclusions: The association between oxidative stress parameters, antioxidant markers, the inflammation index and lipid status parameters suggest their involvement in atherosclerosis development that may lead to CAD progression. Keywords: Antioxidant, Oxidative stress, Coronary artery disease, Inflammation, Nonsmoker, Nondiabetic doi:10.1016/j.clinbiochem.2011.08.384
Poster – [A-10-675-3] Brain derived neurotrophic factor expression in the serum and cerebrospinal fluid of patients with hydrocephalus Farhad Mashayekhia, Ebrahim Mirzajanib a Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran b Department of Biochemistry, Guilan Medical Sciences University, Rasht, Iran E-mail addresses: [email protected] (F. Mashayekhi), [email protected] (E. Mirzajani) Introduction: Brain derived neurotrophic factor (BDNF) has been shown to promote survival of all major neuronal types. BDNF is found in many biological fluids including cerebrospinal fluid (CSF). Hydrocephalus is a condition in which there is an abnormal build-up of CSF within the ventricles and/or subarachnoid spaces. Since CSF is in contact with the extracellular space of the brain, biochemical brain modifications could be reflected in the CSF. The aim of this study was to determine the concentration of CSF BDNF in the patients with hydrocephalus. Methods: A total of 52 samples of CSF from normal and hydrocephalic patients were collected by lumbar puncture. Samples were aged matched between the two groups analyzed. The expression of BDNF in the CSF and serum samples was confirmed by Western blot and enzyme linked immunosorbent assay (ELISA). Results: A western blot analysis using anti-BDNF antibody confirmed the presence of BDNF in all CSF samples. An image analyzer was used to determine the intensities of the band in the respective lanes. Quantification of Western blot gels showed that the amount of BDNF was increased in the hydrocephalus CSF as compared to normal samples. Using ELISA, it was shown that the concentration of CSF BDNF in the hydrocephalus was significantly higher than in normal samples. Conclusion: The data from this study suggest that BDNF is a constant component of human CSF. It is also concluded that increased concentra-
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tion of BDNF in the CSF of patients may be partly related to pathophysiology of the disease. Keywords: Brain derived neurotrophic factor, Serum, Cerebrospinal fluid, Hydrocephalus doi:10.1016/j.clinbiochem.2011.08.385
Poster – [A-10-689-1] Comparison of Anti-Heat Shock Protein 27 Antibody Levels Among Several Groups, from Childhood to Indolence Amir Ali Rahsepar, Shima Tavallaie, Mohsen Moohebati, Fatemeh Moodi, Hamid Abdi, Pajhooh Mohamoud Reza Azar, Amir Hossein Sahebkar, Hossein Pourghadamyari, Majid Ghayour-Mobarhan, Gordon Ferns Biochemical and Nutritional Research Centre, Medical University of Mashhad, Mashhad, Iran E-mail addresses: [email protected] (A.A. Rahsepar), [email protected] (S. Tavallaie) Introduction: We aimed to assess the antibody levels against heat shock protein (Hsp)-27 in several groups aging from childhood to indolence. Materials and Methods: Totally, 782 subjects enrolled to our study, the groups were included 35 healthy children, 179 obese adults, 400 subjects suspicious to coronary artery disease (CAD) (containing 100 and 300 subjects with negative and positive documents for CAD, respectively), 94 subjects with acute coronary syndrome (ACS) and finally 75 subjects with stroke. The anti-Hsp27 antibody levels were measured by in-house enzyme-linked immunosorbent assay (ELISA). Results: Children had very low levels of anti-Hsp27 antibody levels (P< 0.001). The obese healthy subjects have the lower levels of antiHsp27 antibody when compared with those subjects who were suspicious to stable angina (P< 0.001). The CAD negative subjects had lower antibody levels when compared with CAD positive patients (P< 0.001). In patients with ACS and stroke the levels of anti-Hsp27 antibody was lower when compared with CAD positive patients (P< 0.001), but ACS patients had the higher levels of anti-Hsp27 antibody levels when compared with stroke patients (P= 0.003). Discussion: In conclusion, when a child gets older and faces with more cardiovascular risk factors including infection, the level of antiHsp27 antibody rises and peaks by becoming CAD positive. Moreover, the level of anti-Hsp27 antibody levels is more specific for cardiac rather than cerebrovascular events. Keywords: Heat shock protein, Antibody, Cardiovascular disease doi:10.1016/j.clinbiochem.2011.08.386
Oral — [A-10-689-2] Changes in a measure of anti-heat shock protein 27 antibody levels and pro-oxidant–antioxidant balance after bare metal and drug eluting stent implantation in patients undergoing percutaneous coronary intervention Amir Ali Rahsepar, Shima Tavallaie, Mohsen Moohebati, Fatemeh Moodi, Seyyed Mohammad Reza Parizadeh, Hossein Pourghadamyari, Seyyed Mohammad Reza Kazemi Bachestani, Roghayeh Paydar, Homa Falsoleiman, Mashallah Dehghani, Majid Ghayour-Mobarhan, Gordon Ferns Biochemical and Nutritional Research Centre, Mashhad Medical University, Mashhad, Iran E-mail addresses: [email protected] (A.A. Rahsepar), [email protected] (M. Ghayour-Mobarhan)
Introduction: The oxidative stress and inflammation are cooperative events involved in atherosclerosis development. In the present study we assessed the association of MDA, antioxidant markers, high sensitive C -reactive protein (hs-CRP) and lipid status parameters in the patients with coronary artery disease (CAD). Significant risk factors such as cigarette and diabet were excluded from the study. Materials and Methods: Oxidative stress parameters for example Malondialdehyde (MDA), antioxidant markers including: erythrocyte superoxide dismutase (SOD), Glutathion peroxidase (GPX), total antioxidant capacity (TAC), the inflammation marker and serum lipid status parameters were measured in 120 subjects including 60 CAD patients with angiographically diagnosed CAD and 60 CAD-free subjects as a control group, also diabetics, smoking patients, patients with malignancy, renal and liver disease, and other disease were excluded from the study. Results: The serum MDA and hs-CRP levels were increased significantly as compared to controls. However, erythrocyte SOD, GPX activities and TAC level were reduced significantly in patients (P<0.05 in all cases). The levels of total cholesterol, Triglyceride, LDL-C were significantly higher and that of HDL-C was meaningfully lower than those of control (P<0.05 in all cases). Conclusions: The association between oxidative stress parameters, antioxidant markers, the inflammation index and lipid status parameters suggest their involvement in atherosclerosis development that may lead to CAD progression. Keywords: Antioxidant, Oxidative stress, Coronary artery disease, Inflammation, Nonsmoker, Nondiabetic doi:10.1016/j.clinbiochem.2011.08.384
Poster – [A-10-675-3] Brain derived neurotrophic factor expression in the serum and cerebrospinal fluid of patients with hydrocephalus Farhad Mashayekhia, Ebrahim Mirzajanib a Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran b Department of Biochemistry, Guilan Medical Sciences University, Rasht, Iran E-mail addresses: [email protected] (F. Mashayekhi), [email protected] (E. Mirzajani) Introduction: Brain derived neurotrophic factor (BDNF) has been shown to promote survival of all major neuronal types. BDNF is found in many biological fluids including cerebrospinal fluid (CSF). Hydrocephalus is a condition in which there is an abnormal build-up of CSF within the ventricles and/or subarachnoid spaces. Since CSF is in contact with the extracellular space of the brain, biochemical brain modifications could be reflected in the CSF. The aim of this study was to determine the concentration of CSF BDNF in the patients with hydrocephalus. Methods: A total of 52 samples of CSF from normal and hydrocephalic patients were collected by lumbar puncture. Samples were aged matched between the two groups analyzed. The expression of BDNF in the CSF and serum samples was confirmed by Western blot and enzyme linked immunosorbent assay (ELISA). Results: A western blot analysis using anti-BDNF antibody confirmed the presence of BDNF in all CSF samples. An image analyzer was used to determine the intensities of the band in the respective lanes. Quantification of Western blot gels showed that the amount of BDNF was increased in the hydrocephalus CSF as compared to normal samples. Using ELISA, it was shown that the concentration of CSF BDNF in the hydrocephalus was significantly higher than in normal samples. Conclusion: The data from this study suggest that BDNF is a constant component of human CSF. It is also concluded that increased concentra-
S155
tion of BDNF in the CSF of patients may be partly related to pathophysiology of the disease. Keywords: Brain derived neurotrophic factor, Serum, Cerebrospinal fluid, Hydrocephalus doi:10.1016/j.clinbiochem.2011.08.385
Poster – [A-10-689-1] Comparison of Anti-Heat Shock Protein 27 Antibody Levels Among Several Groups, from Childhood to Indolence Amir Ali Rahsepar, Shima Tavallaie, Mohsen Moohebati, Fatemeh Moodi, Hamid Abdi, Pajhooh Mohamoud Reza Azar, Amir Hossein Sahebkar, Hossein Pourghadamyari, Majid Ghayour-Mobarhan, Gordon Ferns Biochemical and Nutritional Research Centre, Medical University of Mashhad, Mashhad, Iran E-mail addresses: [email protected] (A.A. Rahsepar), [email protected] (S. Tavallaie) Introduction: We aimed to assess the antibody levels against heat shock protein (Hsp)-27 in several groups aging from childhood to indolence. Materials and Methods: Totally, 782 subjects enrolled to our study, the groups were included 35 healthy children, 179 obese adults, 400 subjects suspicious to coronary artery disease (CAD) (containing 100 and 300 subjects with negative and positive documents for CAD, respectively), 94 subjects with acute coronary syndrome (ACS) and finally 75 subjects with stroke. The anti-Hsp27 antibody levels were measured by in-house enzyme-linked immunosorbent assay (ELISA). Results: Children had very low levels of anti-Hsp27 antibody levels (P< 0.001). The obese healthy subjects have the lower levels of antiHsp27 antibody when compared with those subjects who were suspicious to stable angina (P< 0.001). The CAD negative subjects had lower antibody levels when compared with CAD positive patients (P< 0.001). In patients with ACS and stroke the levels of anti-Hsp27 antibody was lower when compared with CAD positive patients (P< 0.001), but ACS patients had the higher levels of anti-Hsp27 antibody levels when compared with stroke patients (P= 0.003). Discussion: In conclusion, when a child gets older and faces with more cardiovascular risk factors including infection, the level of antiHsp27 antibody rises and peaks by becoming CAD positive. Moreover, the level of anti-Hsp27 antibody levels is more specific for cardiac rather than cerebrovascular events. Keywords: Heat shock protein, Antibody, Cardiovascular disease doi:10.1016/j.clinbiochem.2011.08.386
Oral — [A-10-689-2] Changes in a measure of anti-heat shock protein 27 antibody levels and pro-oxidant–antioxidant balance after bare metal and drug eluting stent implantation in patients undergoing percutaneous coronary intervention Amir Ali Rahsepar, Shima Tavallaie, Mohsen Moohebati, Fatemeh Moodi, Seyyed Mohammad Reza Parizadeh, Hossein Pourghadamyari, Seyyed Mohammad Reza Kazemi Bachestani, Roghayeh Paydar, Homa Falsoleiman, Mashallah Dehghani, Majid Ghayour-Mobarhan, Gordon Ferns Biochemical and Nutritional Research Centre, Mashhad Medical University, Mashhad, Iran E-mail addresses: [email protected] (A.A. Rahsepar), [email protected] (M. Ghayour-Mobarhan)