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C 04 Leukocyte adhesion molecules in active ocular lesions of Behçet's disease
24 s
6e conference intemationale sur la maladie de Behfet
C03
The phenotypic and functional abnormality of y5 T lymphocytes in Behget's disease N. Yamashita, M. Mochizuki, Y. Mizushima, T. Sakane
The most outstanding feature of Behqet's disease (BD) is the accumulation of neutrophils in the inflammatory lesions. It has been suggested that T lymphocytes play a significant role in generating neutrophil hyperfunction. In this study, we examined phenotypic abnormalities of y8 T lymphocytes, which are distributed in muco-cutaneous lesions and are thought to possess defense mechanisms at the lesions. We found the existence of a unique population of 78 T lymphocytes, which were VqO+Vg2+ CD45RA + ceils. In contrast, most of VqO+V82+ cells were CD45RO+ CD45RA- in healthy control. The increase of V,/9+V82+CD45RA+ cells was most prominently observed in neuro-BD and in some cases these cells increased up to 60% of total T cells. Because CD45 isoforms define the functional subsets of naive and memory T lymphocytes, we investigated mRNA splicing of
CD45 isoforms using RT-PCR methods. We found that 865bp products, which produce a 220kDa protein, were absent in V79+V82+CD45RA+CD45RO- cells in BD. These data suggest that there exists a unique phenotypic 76 T lymphocytes in BD, and some of them appear to be so called "memory type" CD45RA+ cells. We also established 78 T cell lines stimulated by S. Sanguis antigen which is assumed to be one of the pathognomonic extrinsic antigens of BD. These cell lines produced large amounts of TNF-cq TNF~3 and IL-8, indicating that 76 T cells may play a essential role in pathogenesis of BD by priming neutrophils and causing tissue damages. Institute of Medical Science, St. Marianna University, 2-16-1 Sugao, Miyamae-ku, Kawasaki 216, Japan
c04
Leukocyte adhesion molecules in active ocular lesions of Behget's disease G. Inaba ~, H. Kaku 2, H. Ujihara 3, Y. Miyanaga 3, R. Hara 3
The sequential cascades in leukocyte adhesion and migration are important events in the development of inflammatory and immune response in Behqet's disease. In an attempt to clarify the relation of active ocular lesions to inflammatory reactions covering entire body, we have detected LECAM- 1, Mac- 1 and CD44 expressed on the peripheral leukocytes by flow cytometry in 25 patients with BehGet' s disease. LECAM-1 and CD44 expression was dramatically decreased from polymorphonuclear leukocytes (PMN) upon ocular attacks, whereas the changes in Mac-1 appeared not so stricking. This tendency had continued into the recovery stage of ocular inflammation. T lymphocytes showed, on the
other hand, no considerable variability in regard to the expression of cell surface LECAM- 1, CD44 and also of Mac- 1 even at the peak of active ocular inflammation. The results suggest that interaction between PMN and primed vascular endothelial cells might be apt to precede functional modifications of T lymphocytes, and it is also supposed that facilitation of activated PMN recruitment mediated by LECAM- 1 plays an essential role on the development of ocular inflammation in Behqet' s disease. 1)2nd Dpt. of lntemal Medicine, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173, Japan 2) The Kanagawa Rehabilitation Institute, Kanagawa, Japan 3) Dept. of Ophthalmol., Tokyo Women's Medical College, Tokyo, Japan
La Revue de M#decine Interne
6e conference intemationale sur la maladie de Behfet
C03
The phenotypic and functional abnormality of y5 T lymphocytes in Behget's disease N. Yamashita, M. Mochizuki, Y. Mizushima, T. Sakane
The most outstanding feature of Behqet's disease (BD) is the accumulation of neutrophils in the inflammatory lesions. It has been suggested that T lymphocytes play a significant role in generating neutrophil hyperfunction. In this study, we examined phenotypic abnormalities of y8 T lymphocytes, which are distributed in muco-cutaneous lesions and are thought to possess defense mechanisms at the lesions. We found the existence of a unique population of 78 T lymphocytes, which were VqO+Vg2+ CD45RA + ceils. In contrast, most of VqO+V82+ cells were CD45RO+ CD45RA- in healthy control. The increase of V,/9+V82+CD45RA+ cells was most prominently observed in neuro-BD and in some cases these cells increased up to 60% of total T cells. Because CD45 isoforms define the functional subsets of naive and memory T lymphocytes, we investigated mRNA splicing of
CD45 isoforms using RT-PCR methods. We found that 865bp products, which produce a 220kDa protein, were absent in V79+V82+CD45RA+CD45RO- cells in BD. These data suggest that there exists a unique phenotypic 76 T lymphocytes in BD, and some of them appear to be so called "memory type" CD45RA+ cells. We also established 78 T cell lines stimulated by S. Sanguis antigen which is assumed to be one of the pathognomonic extrinsic antigens of BD. These cell lines produced large amounts of TNF-cq TNF~3 and IL-8, indicating that 76 T cells may play a essential role in pathogenesis of BD by priming neutrophils and causing tissue damages. Institute of Medical Science, St. Marianna University, 2-16-1 Sugao, Miyamae-ku, Kawasaki 216, Japan
c04
Leukocyte adhesion molecules in active ocular lesions of Behget's disease G. Inaba ~, H. Kaku 2, H. Ujihara 3, Y. Miyanaga 3, R. Hara 3
The sequential cascades in leukocyte adhesion and migration are important events in the development of inflammatory and immune response in Behqet's disease. In an attempt to clarify the relation of active ocular lesions to inflammatory reactions covering entire body, we have detected LECAM- 1, Mac- 1 and CD44 expressed on the peripheral leukocytes by flow cytometry in 25 patients with BehGet' s disease. LECAM-1 and CD44 expression was dramatically decreased from polymorphonuclear leukocytes (PMN) upon ocular attacks, whereas the changes in Mac-1 appeared not so stricking. This tendency had continued into the recovery stage of ocular inflammation. T lymphocytes showed, on the
other hand, no considerable variability in regard to the expression of cell surface LECAM- 1, CD44 and also of Mac- 1 even at the peak of active ocular inflammation. The results suggest that interaction between PMN and primed vascular endothelial cells might be apt to precede functional modifications of T lymphocytes, and it is also supposed that facilitation of activated PMN recruitment mediated by LECAM- 1 plays an essential role on the development of ocular inflammation in Behqet' s disease. 1)2nd Dpt. of lntemal Medicine, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173, Japan 2) The Kanagawa Rehabilitation Institute, Kanagawa, Japan 3) Dept. of Ophthalmol., Tokyo Women's Medical College, Tokyo, Japan
La Revue de M#decine Interne