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C4d: a pathogenetically relevant marker in kidney transplantation
Symposium:TransplantationPathology / Pathology- Research and Practice 200 (2004) 261-262
Results: An increased P-selectin-ligandexpression on CD4+ effector-memory-lymphocytes was found in inflamed mucosal tissues. The most important finding is that Thl cells generated from FucTVII-/-mice displayed largely reduced immigration into the inflamed gut. Conclusions: Beside the mncosa-specific-integrin/MAdCAM1/VCAM-1 pathway also the endothelial selectins are required for the recruitment of T-effector cells into the inflamed gut mucosa in murine colitis. The role of E-selectin and its ligands is rather inflammation-specificthan skin-specifc, at least in the mouse.
25 Developments and Limits of Liver Transplantation in Germany C. H. BROELSCH, Essen
26 Pancreas Transplant Pathology: Clinicopathologic and Experimental Findings H. NIZZE, P. JONAS, O. DIETZE1, W. SCHARECK2 Institut ftir Pathologie, Universit~it Restock 1Institut fiir Pathologie, LandesklinikenSalzburg 2Klinik und Poliklinik f'ttr Chimrgie, Universit~it Restock
Aims: An overview is given about human pancreas transplant findings due to rejection and nonrejection related causes. A comparison is made with experimental pancreas transplant changes in rats. Methods: 124 transplantations ( 106 simultaneous pancreas kidney, 13 pancreas alone, 5 pancreas after kidney) done in Restock from VI/95 to VI/03 were evaluated in respect to pancreas transplant lesi-ons. Additionally, pancreas specimen of Brown Norway rats experi-mentally transplanted into diabetic Lewis rats were examined. Results: From 55 out of the 124 pancreas transplant patients, 107 partly repeated pancreas graft specimen were studied morphologically. The principal lesions in the transplanted human pancreas were acute (enzymatic) necrotizing transplant pancreatitis (43 samples) and acute graft thrombosis (12 probes) as well as acute (10) and chronic (14) transplant rejection specimen. 26 probes were zero-hour biopsies and 2 showed normal tissue. Acute and chronic rejection findings described in detail can be classified into normal and borderline changes as well as both in three rejection grades (I: mild, II: moderate, III: severe). Experimental acute pancreas transplant rejection in rats showed quite similar findings to human grafts and could also be graded into different rejection stages. Conclusions: As in other grafted organs, the changes occurring in the transplanted pancreas can be ranged into varying categories related and/or not related to pancreas transplant rejection. A concise classification and a reproduceable grading schedule are suggested for diagnostic, therapeutic, and prognostic purposes.
27 Stem cell-mediated tolerance induction in transplantation F. FJ~2,1DRICH,Kiel
261
State, of the a :::lecture:,: Vet TransplantatiO 28
Liver Transplantation
B. PORTMANN, London
29
Posttransplantation lymphoproliferative disorders
H. K. M()LLER-HERMELINK, G. OTT, TH. RODIGER, Wfirzburg
30 Polyoma virus nephropathy: An important and often avoidable complication of modern immunosuppression V. NICKELEIT, Chapel Hill
31
Infections in Transplantation
R. WORZNER, A. GSCHWENDTNER, Innsbruck
32 C4d: A pathogenetically relevant marker in kidney transplantation H. REGELE, Wien
33 First evidence for B-lymphocyte/plasma cell participation in acute liver allograft rejection: B-cell expansion, expression of B-cell specific chemokines and IgVh gene diversification J. MOELLER, M.G. KRUKEMEYER, L. MORAWIETZ, C. RADKE, U. NEUMANN1,T. THERUWAT1, C. BEREW, V. KRENN Institut ftir Pathologie, Universit~itsklinikum Charite, Berlin 1Chirurgische Klinik, Universit~itsklinikum Charite, Berlin 2Deutsches Rheumaforschungszentrum,Berlin
Aims: To find out whether B=lymphocytes and plasma cells expand intrahepatically through migration or local proliferation, and to analyze if B-cell/plasma cell specific chemokines/receptors are expressed in acute liver allograft rejection. Methods: Liver biopsies from 20 patients with acute rejection (AR) were analyzed by immunohistochemical single and double staining (CD20, CD38, CD138 and CD20/Ki67) and compared with biopsies from the same livers prior to implantation (PI). B-cell specific chemokines MIP-3ct CXCL9, CXCL10, CXCLll, CXCL12 and receptors CCR-6 CXCR3 and CXCR4 were detected
Results: An increased P-selectin-ligandexpression on CD4+ effector-memory-lymphocytes was found in inflamed mucosal tissues. The most important finding is that Thl cells generated from FucTVII-/-mice displayed largely reduced immigration into the inflamed gut. Conclusions: Beside the mncosa-specific-integrin/MAdCAM1/VCAM-1 pathway also the endothelial selectins are required for the recruitment of T-effector cells into the inflamed gut mucosa in murine colitis. The role of E-selectin and its ligands is rather inflammation-specificthan skin-specifc, at least in the mouse.
25 Developments and Limits of Liver Transplantation in Germany C. H. BROELSCH, Essen
26 Pancreas Transplant Pathology: Clinicopathologic and Experimental Findings H. NIZZE, P. JONAS, O. DIETZE1, W. SCHARECK2 Institut ftir Pathologie, Universit~it Restock 1Institut fiir Pathologie, LandesklinikenSalzburg 2Klinik und Poliklinik f'ttr Chimrgie, Universit~it Restock
Aims: An overview is given about human pancreas transplant findings due to rejection and nonrejection related causes. A comparison is made with experimental pancreas transplant changes in rats. Methods: 124 transplantations ( 106 simultaneous pancreas kidney, 13 pancreas alone, 5 pancreas after kidney) done in Restock from VI/95 to VI/03 were evaluated in respect to pancreas transplant lesi-ons. Additionally, pancreas specimen of Brown Norway rats experi-mentally transplanted into diabetic Lewis rats were examined. Results: From 55 out of the 124 pancreas transplant patients, 107 partly repeated pancreas graft specimen were studied morphologically. The principal lesions in the transplanted human pancreas were acute (enzymatic) necrotizing transplant pancreatitis (43 samples) and acute graft thrombosis (12 probes) as well as acute (10) and chronic (14) transplant rejection specimen. 26 probes were zero-hour biopsies and 2 showed normal tissue. Acute and chronic rejection findings described in detail can be classified into normal and borderline changes as well as both in three rejection grades (I: mild, II: moderate, III: severe). Experimental acute pancreas transplant rejection in rats showed quite similar findings to human grafts and could also be graded into different rejection stages. Conclusions: As in other grafted organs, the changes occurring in the transplanted pancreas can be ranged into varying categories related and/or not related to pancreas transplant rejection. A concise classification and a reproduceable grading schedule are suggested for diagnostic, therapeutic, and prognostic purposes.
27 Stem cell-mediated tolerance induction in transplantation F. FJ~2,1DRICH,Kiel
261
State, of the a :::lecture:,: Vet TransplantatiO 28
Liver Transplantation
B. PORTMANN, London
29
Posttransplantation lymphoproliferative disorders
H. K. M()LLER-HERMELINK, G. OTT, TH. RODIGER, Wfirzburg
30 Polyoma virus nephropathy: An important and often avoidable complication of modern immunosuppression V. NICKELEIT, Chapel Hill
31
Infections in Transplantation
R. WORZNER, A. GSCHWENDTNER, Innsbruck
32 C4d: A pathogenetically relevant marker in kidney transplantation H. REGELE, Wien
33 First evidence for B-lymphocyte/plasma cell participation in acute liver allograft rejection: B-cell expansion, expression of B-cell specific chemokines and IgVh gene diversification J. MOELLER, M.G. KRUKEMEYER, L. MORAWIETZ, C. RADKE, U. NEUMANN1,T. THERUWAT1, C. BEREW, V. KRENN Institut ftir Pathologie, Universit~itsklinikum Charite, Berlin 1Chirurgische Klinik, Universit~itsklinikum Charite, Berlin 2Deutsches Rheumaforschungszentrum,Berlin
Aims: To find out whether B=lymphocytes and plasma cells expand intrahepatically through migration or local proliferation, and to analyze if B-cell/plasma cell specific chemokines/receptors are expressed in acute liver allograft rejection. Methods: Liver biopsies from 20 patients with acute rejection (AR) were analyzed by immunohistochemical single and double staining (CD20, CD38, CD138 and CD20/Ki67) and compared with biopsies from the same livers prior to implantation (PI). B-cell specific chemokines MIP-3ct CXCL9, CXCL10, CXCLll, CXCL12 and receptors CCR-6 CXCR3 and CXCR4 were detected