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Cadaverine effect on microtubule polymerization
S266
Abstracts
Poster – [A-10-1131-1] Comparing serum Follicle-Stimulating Hormone (FSH) level with vaginal pH in women with menopausal symptoms Zohreh Yousefi, Shohreh Saeed, Fatemeh Vahidroodsari, Sedigheh Ayati Ghaem Hospital, School of Medical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (Z. Yousefi), [email protected] (S. Saeed), [email protected] (F. Vahidroodsari), [email protected] (S. Ayati)
Materials and methods: Tubulin was extracted from brain sheep and was conducted by adding 1 mM GTP to tubulin in PEM buffer at 37 ° C for 10–12 min in the presence and absence of cadaverine. Results: The results showed that cadaverine relatively enhanced microtubule polymerization rate at low concentrations (micromolar). But it compeletely inhibited the polymerization with increasing concentration up to millimolars. Moreover, nucleation time of tubuline polymerization increased with increasing concentrations of cadaverine. Conclusion: Therefore, according to our observations, effect of cadaverine on microtubule polymerization showed concentration dependency.
Introduction: Despite the important implication for women's health and reproduction, very few studies have focused on vaginal pH for menopausal diagnosis. Recent studies have suggested vaginal pH as a simple, noninvasive and inexpensive method for this purpose. Objective: The aim of this study is to compare serum FSH level with vaginal PH in menopause. Methods: This is a cross-sectional, descriptive study, conducted on 103 women (aged 31–95 yrs) with menopausal symptoms who were referred to the Menopausal Clinic at Ghaem Hospital during 2006. Vaginal pH was measured using pH meter strips and serum FSH levels were measured using immunoassay methods. The data was analyzed using SPSS software (version 11.5) and results were evaluated statistically by the Chi-square and Kappa tests. p≤0.05 was considered statistically significant. Results: According to this study, in the absence of vaginal infection, the average vaginal pH in these 103 menopausal women was 5.33±0.53. If the menopausal hallmark was considered as vaginal pH>4.5, and serum FSH as ≥20 mIU/ml, then the sensitivity of vaginal pH for menopausal diagnosis was 97%. The mean of FSH levels in this population was 80.79 mIU/ml. Conclusion: Vaginal pH is a simple, accurate, and cost effective tool that can be suggested as a suitable alternative to serum FSH measurement for the diagnosis of menopause.
Keywords: Microtubule, Polymerization, Cadaverine, Nucleation
Keywords: FSH, Vaginal pH, Menopausal symptoms doi:10.1016/j.clinbiochem.2011.08.920
Poster – [A-10-1134-1] Cadaverine effect on microtubule polymerization Tayebe Cheraghi-Shavia, Roya Mahinpurb, Gholamhosein Riazic, Zahra Moosavi-Nejadd a Tehran, Alzahra University, Science Faculty Biology Department, Iran b Faculty of Science, Kashan University, Kashan, Iran c Tehran, Tehran University, Institute of Biochemistry and Biophysics IBB, Iran d Biology Department, Iran E-mail addresses: [email protected] (T. Cheraghi-Shavi), [email protected] (R. Mahinpur), [email protected] (G. Riazi) Introduction: Microtubules are key components of the cytoskeleton of eukaryotic cells and play important roles in many aspects of biological functions including intracellular trafficking of vesicles and organelles, cellular motility and mitotic chromosome segregation. Microtubules are highly dynamic structures whose regulation is crucial for variety of cellular processes. Several molecules have been shown to affect the kinetics of the process. Some kinetically active substances may affect the structural pattern of the polymer. Development of microtubule inhibitors, which interfere with microtubule assembly and disassembly, is useful for the investigation of the biological function of microtubules. In this study, effect of cadaverine as a biological ligand has been investigated on microtubule polymerization.
doi:10.1016/j.clinbiochem.2011.08.921
Poster – [A-10-1135-1] Spectrophotometric investigation of copper (II)–erythromycin interaction Hillsa Bidkhori, Raziyeh Sanavikhoshnood, Zarin Eshaghi Department of Chemistry, Mashhad Branch, Islamic Azad University of Mashhad, Mashhad, Iran E-mail addresses: [email protected] (H. Bidkhori), [email protected] (R. Sanavikhoshnood), [email protected] (Z. Eshaghi) Erythromycin is a macrolid antibiotic consisting of a 14 member ring , a keton group, two glycosidic bonds and dimethylamino group. The drug targets the ribosome and inhibits the protein synthesis of Gram positive bacteria. In this work, we studied the interaction of erythromycin with Cu2+ cation in methanol solvent and determined the formation constant of the possible complex that can be produced by this interaction. Clinical work showed that the concurrent ingestion of antibiotics containing multivalent cations could reduce the bioavailability by up to 90%. Job's method of continuous variation was employed. The interaction of erythromycin with Cu2+ was studied by preparation of a series of methanolic solutions with variation concentraction of ligand and cation. Measurment on complex was done at the wavelength of 340 nm and the influence of pH, time and interferences has been tested. The interaction of erythromycin with Cu2+ has been found to form one complex with metal to ligand composition of 1:2 and the formation constant was obtained to indicate that the complex is stable. Keywords: Spectrophotometry, Erythromycin, Interaction, Cu2+ doi:10.1016/j.clinbiochem.2011.08.922
Poster – [A-10-1157-1] Effects of NaSCN and KSCN on fatty acid absorption by E.G.S from small intestinal of sheep Heshmatallah Oroojia, Mohsen Anib, Seyed Aliasghar Moshtaghib a Najafabad Azad University, Najafabd, Iran b Department of Biochemistry, Isfahan University of Medical Sciences, Isfahan, Iran E-mail addresses: [email protected] (H. Orooji), [email protected] (S.A. Moshtaghi) Introduction: Fatty acids are lipophilic molecules which were believed that they can pass easily across the membrane. But studies revealed that fatty acid transport occurs by special transporters which are present in many tissues. More studies showed that for this transport Na+ is also needed so that in the absence of Na+, fatty acid uptake is
Abstracts
Poster – [A-10-1131-1] Comparing serum Follicle-Stimulating Hormone (FSH) level with vaginal pH in women with menopausal symptoms Zohreh Yousefi, Shohreh Saeed, Fatemeh Vahidroodsari, Sedigheh Ayati Ghaem Hospital, School of Medical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (Z. Yousefi), [email protected] (S. Saeed), [email protected] (F. Vahidroodsari), [email protected] (S. Ayati)
Materials and methods: Tubulin was extracted from brain sheep and was conducted by adding 1 mM GTP to tubulin in PEM buffer at 37 ° C for 10–12 min in the presence and absence of cadaverine. Results: The results showed that cadaverine relatively enhanced microtubule polymerization rate at low concentrations (micromolar). But it compeletely inhibited the polymerization with increasing concentration up to millimolars. Moreover, nucleation time of tubuline polymerization increased with increasing concentrations of cadaverine. Conclusion: Therefore, according to our observations, effect of cadaverine on microtubule polymerization showed concentration dependency.
Introduction: Despite the important implication for women's health and reproduction, very few studies have focused on vaginal pH for menopausal diagnosis. Recent studies have suggested vaginal pH as a simple, noninvasive and inexpensive method for this purpose. Objective: The aim of this study is to compare serum FSH level with vaginal PH in menopause. Methods: This is a cross-sectional, descriptive study, conducted on 103 women (aged 31–95 yrs) with menopausal symptoms who were referred to the Menopausal Clinic at Ghaem Hospital during 2006. Vaginal pH was measured using pH meter strips and serum FSH levels were measured using immunoassay methods. The data was analyzed using SPSS software (version 11.5) and results were evaluated statistically by the Chi-square and Kappa tests. p≤0.05 was considered statistically significant. Results: According to this study, in the absence of vaginal infection, the average vaginal pH in these 103 menopausal women was 5.33±0.53. If the menopausal hallmark was considered as vaginal pH>4.5, and serum FSH as ≥20 mIU/ml, then the sensitivity of vaginal pH for menopausal diagnosis was 97%. The mean of FSH levels in this population was 80.79 mIU/ml. Conclusion: Vaginal pH is a simple, accurate, and cost effective tool that can be suggested as a suitable alternative to serum FSH measurement for the diagnosis of menopause.
Keywords: Microtubule, Polymerization, Cadaverine, Nucleation
Keywords: FSH, Vaginal pH, Menopausal symptoms doi:10.1016/j.clinbiochem.2011.08.920
Poster – [A-10-1134-1] Cadaverine effect on microtubule polymerization Tayebe Cheraghi-Shavia, Roya Mahinpurb, Gholamhosein Riazic, Zahra Moosavi-Nejadd a Tehran, Alzahra University, Science Faculty Biology Department, Iran b Faculty of Science, Kashan University, Kashan, Iran c Tehran, Tehran University, Institute of Biochemistry and Biophysics IBB, Iran d Biology Department, Iran E-mail addresses: [email protected] (T. Cheraghi-Shavi), [email protected] (R. Mahinpur), [email protected] (G. Riazi) Introduction: Microtubules are key components of the cytoskeleton of eukaryotic cells and play important roles in many aspects of biological functions including intracellular trafficking of vesicles and organelles, cellular motility and mitotic chromosome segregation. Microtubules are highly dynamic structures whose regulation is crucial for variety of cellular processes. Several molecules have been shown to affect the kinetics of the process. Some kinetically active substances may affect the structural pattern of the polymer. Development of microtubule inhibitors, which interfere with microtubule assembly and disassembly, is useful for the investigation of the biological function of microtubules. In this study, effect of cadaverine as a biological ligand has been investigated on microtubule polymerization.
doi:10.1016/j.clinbiochem.2011.08.921
Poster – [A-10-1135-1] Spectrophotometric investigation of copper (II)–erythromycin interaction Hillsa Bidkhori, Raziyeh Sanavikhoshnood, Zarin Eshaghi Department of Chemistry, Mashhad Branch, Islamic Azad University of Mashhad, Mashhad, Iran E-mail addresses: [email protected] (H. Bidkhori), [email protected] (R. Sanavikhoshnood), [email protected] (Z. Eshaghi) Erythromycin is a macrolid antibiotic consisting of a 14 member ring , a keton group, two glycosidic bonds and dimethylamino group. The drug targets the ribosome and inhibits the protein synthesis of Gram positive bacteria. In this work, we studied the interaction of erythromycin with Cu2+ cation in methanol solvent and determined the formation constant of the possible complex that can be produced by this interaction. Clinical work showed that the concurrent ingestion of antibiotics containing multivalent cations could reduce the bioavailability by up to 90%. Job's method of continuous variation was employed. The interaction of erythromycin with Cu2+ was studied by preparation of a series of methanolic solutions with variation concentraction of ligand and cation. Measurment on complex was done at the wavelength of 340 nm and the influence of pH, time and interferences has been tested. The interaction of erythromycin with Cu2+ has been found to form one complex with metal to ligand composition of 1:2 and the formation constant was obtained to indicate that the complex is stable. Keywords: Spectrophotometry, Erythromycin, Interaction, Cu2+ doi:10.1016/j.clinbiochem.2011.08.922
Poster – [A-10-1157-1] Effects of NaSCN and KSCN on fatty acid absorption by E.G.S from small intestinal of sheep Heshmatallah Oroojia, Mohsen Anib, Seyed Aliasghar Moshtaghib a Najafabad Azad University, Najafabd, Iran b Department of Biochemistry, Isfahan University of Medical Sciences, Isfahan, Iran E-mail addresses: [email protected] (H. Orooji), [email protected] (S.A. Moshtaghi) Introduction: Fatty acids are lipophilic molecules which were believed that they can pass easily across the membrane. But studies revealed that fatty acid transport occurs by special transporters which are present in many tissues. More studies showed that for this transport Na+ is also needed so that in the absence of Na+, fatty acid uptake is