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Capillary and myofiber ultrastructure in endomyocardial biopsies from cyclosporin-treated heart transplant patients
430
4bstracts of The Israel Society of Electron Microscopy
included damaged mitochondria, swollen rough endoplas~ic reticulum and nuclear shape deformation. Pre]tminary results of the present study sncw no significant increase of degeneration i n s t r i a vascularis when com~redwith our p r i o r studies on the effects of both drugs administered alone. C A P I L L A R Y AND M Y O F I B E R U L T R A S T R U C T U R E IN E N D O M Y O C A R D I A L B I O P S I E S FROM CYCLOSPORIMT R E A T E D H~ART T R A N S P L A N T P A T I E N T S
Rena Yarom, Dan Admon, Morris Mosseri, Dan Sapoznikov and Mervyn S. Gotsman D e p a r t m e n t s of P a t h o l o g y a n d C a r d i o l ogy, Hebrew University - Hadassah Medical School, Jerusalem, Israel
This paper focuses on the ultrastructure of microvessels and myofibers in repeated endomyocardial biopsies from ii patients after heart transplantation treated with cyclosporin. Severe capillary pathology was invariably present w i t h histological signs of rejection but ~ould also be seen in biopsies devoid of lymphocytic infiltration. It is possible that in the latter complement fixing humoral antibodies to vascular endothelial cell antigens are responsible for the capillary damage and sometimes graft failure. The vascular changes appeared to have a good correlation to myofiber pathology. In the absence of the classical signs of rejection, electron microscopy could be used to advantage in detect~ q vascular, non-HLA-related changes influencing transplant survival.
of the pathogenic strain, Tohama I, does not exhibit crystalline ordering. Negatively stained cell envelopes show optical diffraction patterns with well ordered reflections that index according to an orthorhombic unit cell: a = 8.3 ma, b = 14.3 nm. Computer filtering of these micrographs reveals triplets of staln-penetrable indentations (channels) reminiscent of the matrix porin lattice seen in SDS-extracted envelopes of E. coli, or crystalline membrane sheets of other porins assembled in vitro. The symmetry of the B. pertussls lattice is p2, with the rectangular unit cell c o n taining two such triplets in different orientations. This symmetry, determined from negatively stained specimens, is reconciled with the hexagonal net shown after shadowing, in that the latter data do not distinguish between these two orientations. When envelopes are extracted with Zwittergent-3,14, the lattice remains and the m a j o r protein retalned is a 4DK component whose mobility on SDS-PAGE is markedly heat modifiable. We conclude that this lattice is composed of trimers of the 40K porin recently shown by others to be anion selective. Examination of specimens negatively stained with sodium phosphotungstate when compared to uranyl acetate gives the appearance of greater stain accumulation in the triplet indentations, which is consistent with its character of a cationic stain. ULTRASTRUCTURAL LOCALIZATION OF APOLIPOPROTEIN A-~ IN REGENERATING FISH OPTIC NERVE M. Hernandez, M. Schwartz
A NATURALLY OCCURRING CRYSTALLINE PORIN IN THE OUTER MEMBRANE OF BO~DETELLA PERTUSSIS M. Kessel*, M.J. Brennan, B. L. Trus, M. E. Bisher and A. C. Steven N I H and FDA, B e t h e s d a , Md. USA; *and H e b r e w U n i v e r s l t y - H a d a s s a h Medical S c h o o l , J e r u s a l e m , Israel
We have observee a periodic structure on the outer surfaces of the non-pathogenic B. pertussis strains, Tohama III and 10109, by electron microscopy of bacterial whole mounts and of cell envelope preparations contrasted by low-angle rotary shadowing. The cells appear covered with a quasi-hexagonal lattice whose periodicity is 8.3 nm. The surface
A. Harel,
M. Fainaru and
The W e i z m a n n I n s t l t u t e Dept. of Neurobiolog~,
of Science, Re~vot, Iszael
Injury to axons of regenerating nerves causes changes in synthesis of diffusible substances originating from the nonneuronal cells. In the rat sciatic nerve injury induces an increase in accumulation of a 37 kDa polypeptide which was identified as a form of apollpoprotein E (apo E% presumably involved in lipid (cholesterol) transport. In fish, in media-conditioned (CM) by regeneratin 5 optic nerves, a 28 KDa polypeptide was recently identified as apolipoprotein A-I (apo A-I). Immunocytochemical studies revealed the presence of apo A-1 immunoreactive sites in the fish optic nerve. Higher
4bstracts of The Israel Society of Electron Microscopy
included damaged mitochondria, swollen rough endoplas~ic reticulum and nuclear shape deformation. Pre]tminary results of the present study sncw no significant increase of degeneration i n s t r i a vascularis when com~redwith our p r i o r studies on the effects of both drugs administered alone. C A P I L L A R Y AND M Y O F I B E R U L T R A S T R U C T U R E IN E N D O M Y O C A R D I A L B I O P S I E S FROM CYCLOSPORIMT R E A T E D H~ART T R A N S P L A N T P A T I E N T S
Rena Yarom, Dan Admon, Morris Mosseri, Dan Sapoznikov and Mervyn S. Gotsman D e p a r t m e n t s of P a t h o l o g y a n d C a r d i o l ogy, Hebrew University - Hadassah Medical School, Jerusalem, Israel
This paper focuses on the ultrastructure of microvessels and myofibers in repeated endomyocardial biopsies from ii patients after heart transplantation treated with cyclosporin. Severe capillary pathology was invariably present w i t h histological signs of rejection but ~ould also be seen in biopsies devoid of lymphocytic infiltration. It is possible that in the latter complement fixing humoral antibodies to vascular endothelial cell antigens are responsible for the capillary damage and sometimes graft failure. The vascular changes appeared to have a good correlation to myofiber pathology. In the absence of the classical signs of rejection, electron microscopy could be used to advantage in detect~ q vascular, non-HLA-related changes influencing transplant survival.
of the pathogenic strain, Tohama I, does not exhibit crystalline ordering. Negatively stained cell envelopes show optical diffraction patterns with well ordered reflections that index according to an orthorhombic unit cell: a = 8.3 ma, b = 14.3 nm. Computer filtering of these micrographs reveals triplets of staln-penetrable indentations (channels) reminiscent of the matrix porin lattice seen in SDS-extracted envelopes of E. coli, or crystalline membrane sheets of other porins assembled in vitro. The symmetry of the B. pertussls lattice is p2, with the rectangular unit cell c o n taining two such triplets in different orientations. This symmetry, determined from negatively stained specimens, is reconciled with the hexagonal net shown after shadowing, in that the latter data do not distinguish between these two orientations. When envelopes are extracted with Zwittergent-3,14, the lattice remains and the m a j o r protein retalned is a 4DK component whose mobility on SDS-PAGE is markedly heat modifiable. We conclude that this lattice is composed of trimers of the 40K porin recently shown by others to be anion selective. Examination of specimens negatively stained with sodium phosphotungstate when compared to uranyl acetate gives the appearance of greater stain accumulation in the triplet indentations, which is consistent with its character of a cationic stain. ULTRASTRUCTURAL LOCALIZATION OF APOLIPOPROTEIN A-~ IN REGENERATING FISH OPTIC NERVE M. Hernandez, M. Schwartz
A NATURALLY OCCURRING CRYSTALLINE PORIN IN THE OUTER MEMBRANE OF BO~DETELLA PERTUSSIS M. Kessel*, M.J. Brennan, B. L. Trus, M. E. Bisher and A. C. Steven N I H and FDA, B e t h e s d a , Md. USA; *and H e b r e w U n i v e r s l t y - H a d a s s a h Medical S c h o o l , J e r u s a l e m , Israel
We have observee a periodic structure on the outer surfaces of the non-pathogenic B. pertussis strains, Tohama III and 10109, by electron microscopy of bacterial whole mounts and of cell envelope preparations contrasted by low-angle rotary shadowing. The cells appear covered with a quasi-hexagonal lattice whose periodicity is 8.3 nm. The surface
A. Harel,
M. Fainaru and
The W e i z m a n n I n s t l t u t e Dept. of Neurobiolog~,
of Science, Re~vot, Iszael
Injury to axons of regenerating nerves causes changes in synthesis of diffusible substances originating from the nonneuronal cells. In the rat sciatic nerve injury induces an increase in accumulation of a 37 kDa polypeptide which was identified as a form of apollpoprotein E (apo E% presumably involved in lipid (cholesterol) transport. In fish, in media-conditioned (CM) by regeneratin 5 optic nerves, a 28 KDa polypeptide was recently identified as apolipoprotein A-I (apo A-I). Immunocytochemical studies revealed the presence of apo A-1 immunoreactive sites in the fish optic nerve. Higher