- Email: [email protected]
Capsicum peppers from a collection of parents and hybrids
38
Abstracts / Journal of Biotechnology 161S (2012) 19–48
multiplication inside the macrophages, causing systemic dissemination and contamination of the forming egg, by reaching the reproductive tract, for which it expresses a high tropism. The aim of this paper was to review the different virulence-related mechanisms that make possible the contamination of table eggs by S. enteritidis.
females. Weighting of thighs revealed mean vales of 700.94 ± 2.85 g for males and 448.38 ± 1.32 g for females, recording very significant differences between batches average. The obtained data show that the used rearing and slaughtering technologies are healthy. http://dx.doi.org/10.1016/j.jbiotec.2012.07.116
http://dx.doi.org/10.1016/j.jbiotec.2012.07.114 Food and Agricultural Products Capsicum peppers from a collection of parents and hybrids Estela Moreno 1 , Maria Carmen González-Más 2 , Ana Fita 1,∗ , Adrián Rodríguez-Burruezo 1 1
COMAV, Universitat Politècnica de València, Camino Vera s/n, Valencia 46022, Spain 2 IVIA-Fundación Agroalimed, Carretera Moncada-Náquera km 5, Moncada 46113 Spain E-mail address: anfi[email protected] (A. Fita). Capsicum fruits are utilized in many recipes, contributing with their aroma and/or pungency. While pungency has been profusely studied, knowledge on the aroma/volatile fraction is almost nil in terms of breeding and genetics. Here we present the preliminary results of a study about the volatile fraction of fully ripe fruits from a collection of parent lines and hybrids between them. SPME-GC/MS analyses were performed and total terpenoids, esters and alkanes were identified by mass spectra and estimated as GC-peak area units (p.a.u.). In most accessions terpenoids were the most abundant volatiles, ranging between 1.27 and 225.39 × 106 p.a.u., followed by alkanes (0.1–84.0 × 106) and esters (0–35.8 × 106). The same was true for hybrids (terpenoids: 4.5–522.4 × 106, alkanes: 1.2–158.7 × 106, esters: 0–110.2 × 106). Furthermore, a remarkable parent effect was found on the hybrid population, revealed as intermediate or alternatively, transgressive inheritance, although there was no compound-specificity for these models of inheritance. Our findings suggest that hybridization may help breeders to improve the volatile fraction of chile peppers. http://dx.doi.org/10.1016/j.jbiotec.2012.07.115
Phytotoxic effects of herbicide Attribut and surfactant BioPower on the root, stem and leaf anatomy of Triticum aestivum L. Gulden Yilmaz ∗ , Feruzan Dane Department of Biology, Faculty of Science, Trakya University, Edirne 22030, Turkey E-mail address: [email protected] (G. Yilmaz). Herbicide and surfactant compounds that, despite their benefits, may produce a wide range of toxic effects to the environment. This study was performed in order to determine some of toxic effects of different doses of Attribut used in wheat fields and of BioPower surfactant added Attribut on Triticum aestivum L. 0.42, 0.82, 1.68 mM Attribut and the same doses including 0.25% of BioPower surfactant were studied. EC (effective concentration) value corresponded to farmer dose (0.42 mM Attribut). Applications were done by seed treatment. The effects of each doses applied were monitored in terms of anatomical parameters. In conclusion, application of (0.82 mM Attribut) dose and more of that concentration effected anatomy of wheat negatively and these toxic effects increased in use with BioPower considering Attribute deposition in soil. As a response to the stress, narrowing in diameter in the general structure of the root, cuticle thickening, cleavages in parenchyma cells and cell wall thickening in the sclerenchyma cells and endodermis were determined. Similar results were seen in the literature and were discussed. http://dx.doi.org/10.1016/j.jbiotec.2012.07.117
The life span of Listeria monocytogenes in kefir microflora Güven Uraz ∗ , Sezer Akkuzu, Lale Türkmen, Elif Orhan Department of Biology, Faculty of Science, Gazi University, Teknikokullar Ankara 06500, Turkey
Research regarding study of meat quantitative and qualitative production obtained from Ross-308 hybrid, reared at S.C. Fermador SRL, Romania M.M. Ciobanu ∗ , P.C. Boisteanu, Roxana Lazar University of Agricultural Sciences and Veterinary Medicine, 3 Sadoveanu Alley, IASI-700490, Romania E-mail address: [email protected] (M.M. Ciobanu). The results presented in the current study are from a vast series of research and aimed to enlarge the knowledge area regarding qualitative and quantitative characteristics of meat obtained from Ross-308 hybrid reared and slaughtered at S.C. Fermador SRL farm. Determinations were effectuated on a number of 40 individuals (separately for males and females). The main studied indicators were: live weight, carcasses weight, slaughtered yield and rate of the slaughtered regions in carcasses’ composition. Slaughtered yield revealed mean values, 71.64 ± 0.34% at males and 76.95 ± 0.13% at females; between batches’ average being recorded statistical differences very significant (p > 0.001). The variation coefficient was 1.99% for males and 1.49% for females, fact which show the existence of a very homogenous character. Breast represented 523.59 ± 2.67 g for males and 331.09 ± 3.37 g at
E-mail address: [email protected] (G. Uraz). In this research, lactic bacteria and yeasts are isolated and identified from 60 kefir samples in 96-h. 2.0 × 102 CFU/ml of Listeria monocytogenes is inoculated in kefir samples. The life span of Listeria monocytogenes is evaluated inside the kefir samples. To isolate lactic bacteria, yeasts and Listeria monocytogenes, De Man Rogosa Sharpe agar, Potato Dextrose agar, Listeria selective agar base were used respectively. After 24 h incubation Listeria monocytogenes was counted as 1.5 × 106 CFU/ml, after 96 h incubation Listeria monocytogenes wasn’t in kefir samples. There was a decrease in number of lactic bacteria and yeast. Candida kefir and Streptococcus lactis was isolated from control kefir samples all time period. After incubation periods of 24 h Streptococcus lactis and Streptococcus thermophilus, 48 and 72 h Streptococcus lactis and Enterococcus faecium and at 96 h of incubations Enterococcus faecium were isolated from inoculated kefir samples. As the result lactic bacteria and yeast which is in kefir microflora has an effect on growth of pathogens. http://dx.doi.org/10.1016/j.jbiotec.2012.07.118
Abstracts / Journal of Biotechnology 161S (2012) 19–48
multiplication inside the macrophages, causing systemic dissemination and contamination of the forming egg, by reaching the reproductive tract, for which it expresses a high tropism. The aim of this paper was to review the different virulence-related mechanisms that make possible the contamination of table eggs by S. enteritidis.
females. Weighting of thighs revealed mean vales of 700.94 ± 2.85 g for males and 448.38 ± 1.32 g for females, recording very significant differences between batches average. The obtained data show that the used rearing and slaughtering technologies are healthy. http://dx.doi.org/10.1016/j.jbiotec.2012.07.116
http://dx.doi.org/10.1016/j.jbiotec.2012.07.114 Food and Agricultural Products Capsicum peppers from a collection of parents and hybrids Estela Moreno 1 , Maria Carmen González-Más 2 , Ana Fita 1,∗ , Adrián Rodríguez-Burruezo 1 1
COMAV, Universitat Politècnica de València, Camino Vera s/n, Valencia 46022, Spain 2 IVIA-Fundación Agroalimed, Carretera Moncada-Náquera km 5, Moncada 46113 Spain E-mail address: anfi[email protected] (A. Fita). Capsicum fruits are utilized in many recipes, contributing with their aroma and/or pungency. While pungency has been profusely studied, knowledge on the aroma/volatile fraction is almost nil in terms of breeding and genetics. Here we present the preliminary results of a study about the volatile fraction of fully ripe fruits from a collection of parent lines and hybrids between them. SPME-GC/MS analyses were performed and total terpenoids, esters and alkanes were identified by mass spectra and estimated as GC-peak area units (p.a.u.). In most accessions terpenoids were the most abundant volatiles, ranging between 1.27 and 225.39 × 106 p.a.u., followed by alkanes (0.1–84.0 × 106) and esters (0–35.8 × 106). The same was true for hybrids (terpenoids: 4.5–522.4 × 106, alkanes: 1.2–158.7 × 106, esters: 0–110.2 × 106). Furthermore, a remarkable parent effect was found on the hybrid population, revealed as intermediate or alternatively, transgressive inheritance, although there was no compound-specificity for these models of inheritance. Our findings suggest that hybridization may help breeders to improve the volatile fraction of chile peppers. http://dx.doi.org/10.1016/j.jbiotec.2012.07.115
Phytotoxic effects of herbicide Attribut and surfactant BioPower on the root, stem and leaf anatomy of Triticum aestivum L. Gulden Yilmaz ∗ , Feruzan Dane Department of Biology, Faculty of Science, Trakya University, Edirne 22030, Turkey E-mail address: [email protected] (G. Yilmaz). Herbicide and surfactant compounds that, despite their benefits, may produce a wide range of toxic effects to the environment. This study was performed in order to determine some of toxic effects of different doses of Attribut used in wheat fields and of BioPower surfactant added Attribut on Triticum aestivum L. 0.42, 0.82, 1.68 mM Attribut and the same doses including 0.25% of BioPower surfactant were studied. EC (effective concentration) value corresponded to farmer dose (0.42 mM Attribut). Applications were done by seed treatment. The effects of each doses applied were monitored in terms of anatomical parameters. In conclusion, application of (0.82 mM Attribut) dose and more of that concentration effected anatomy of wheat negatively and these toxic effects increased in use with BioPower considering Attribute deposition in soil. As a response to the stress, narrowing in diameter in the general structure of the root, cuticle thickening, cleavages in parenchyma cells and cell wall thickening in the sclerenchyma cells and endodermis were determined. Similar results were seen in the literature and were discussed. http://dx.doi.org/10.1016/j.jbiotec.2012.07.117
The life span of Listeria monocytogenes in kefir microflora Güven Uraz ∗ , Sezer Akkuzu, Lale Türkmen, Elif Orhan Department of Biology, Faculty of Science, Gazi University, Teknikokullar Ankara 06500, Turkey
Research regarding study of meat quantitative and qualitative production obtained from Ross-308 hybrid, reared at S.C. Fermador SRL, Romania M.M. Ciobanu ∗ , P.C. Boisteanu, Roxana Lazar University of Agricultural Sciences and Veterinary Medicine, 3 Sadoveanu Alley, IASI-700490, Romania E-mail address: [email protected] (M.M. Ciobanu). The results presented in the current study are from a vast series of research and aimed to enlarge the knowledge area regarding qualitative and quantitative characteristics of meat obtained from Ross-308 hybrid reared and slaughtered at S.C. Fermador SRL farm. Determinations were effectuated on a number of 40 individuals (separately for males and females). The main studied indicators were: live weight, carcasses weight, slaughtered yield and rate of the slaughtered regions in carcasses’ composition. Slaughtered yield revealed mean values, 71.64 ± 0.34% at males and 76.95 ± 0.13% at females; between batches’ average being recorded statistical differences very significant (p > 0.001). The variation coefficient was 1.99% for males and 1.49% for females, fact which show the existence of a very homogenous character. Breast represented 523.59 ± 2.67 g for males and 331.09 ± 3.37 g at
E-mail address: [email protected] (G. Uraz). In this research, lactic bacteria and yeasts are isolated and identified from 60 kefir samples in 96-h. 2.0 × 102 CFU/ml of Listeria monocytogenes is inoculated in kefir samples. The life span of Listeria monocytogenes is evaluated inside the kefir samples. To isolate lactic bacteria, yeasts and Listeria monocytogenes, De Man Rogosa Sharpe agar, Potato Dextrose agar, Listeria selective agar base were used respectively. After 24 h incubation Listeria monocytogenes was counted as 1.5 × 106 CFU/ml, after 96 h incubation Listeria monocytogenes wasn’t in kefir samples. There was a decrease in number of lactic bacteria and yeast. Candida kefir and Streptococcus lactis was isolated from control kefir samples all time period. After incubation periods of 24 h Streptococcus lactis and Streptococcus thermophilus, 48 and 72 h Streptococcus lactis and Enterococcus faecium and at 96 h of incubations Enterococcus faecium were isolated from inoculated kefir samples. As the result lactic bacteria and yeast which is in kefir microflora has an effect on growth of pathogens. http://dx.doi.org/10.1016/j.jbiotec.2012.07.118