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Cardiac derived inflammatory mediators
J Mol Cell Cardiol21
(Supplement
II) (1989)
S.A. Elgebaly, A. Cohen, M. Allam, N. Yekta. 51 1 CARDIAC DERIVED INFLAMMATORY MEDIATORS. Department of Surgery, University of Connecticut School of Medicine, Farmington, Connecticut, U.S.A. Recent studies from our laboratory have indicated the release of neutrophil chemotactic factors (NCF) from isolated rabbit hearts perfused with cardioplegia (Am Co11 Surg 38:276, 1987). These factors are protein in nature, heat unstable, not extractand exhibit a molecular weight able F organic solvents (i.e., not leukotrienes), value of greater than 100 Kd. The purpose of this study is to test the hypothesis that NCF released from isolated hearts will not only stimulate neutrophil recruitment Rabbit to the heart, but will also activate neutrophils to secrete lysosomal enzymes. peritoneal neutrophils were therefore incubated with the high molecular weight cardiac derived NCF for 5 minutes. Supernatant solutions were then removed and assayed for Results are expressed as percent of maximum the enzyme N-acetyl-B-glucosaminidase. We found that cardiac NCF stimulated the rerelease as determined by lysed calls. lease of 4053% of the enzyme. The standard chemotactic factor f-Met-Leu-Phe induced 4624%. The cytoplasmic enzyme lactic dehydrogenase was not liberated, indicating that the lysosomal enzyme was actively secreted and not released subsequent to cell lysis. These studies further support a role of ischemic myocardial tissues in cardiac inflammation by releasing inflammatory mediators which recruit neutrophils from the circulation to the heart and activate them to release damaging enzymes.
5 12 EFFECT OF THE AQUEOUS EXTRACT OF DELPHMIUM DENUDATUM WALL AGAINST RADIATION INDUCED CHANGES IN RATS MYOCARWJh4. Khan, A.B. A.K. Tibbiya College, A.M.U., Aligarh, India. Earlier studies in our laboratory showed evidence of dose related acute injury in the myocardium after exposure of rats to ionizing radiation. In further continuation of this study, the effects of intervention by pre-treatment with the aqueous extract of pelnhinium denudatum wall have been studies. The study was carried out in healthy albino rats ofeithersex weighing between 150-200 grams. Thetestgroup ofrats was treated with theaqueousextractof Delnhiniurn denudatumwtiora.lly inthedosesoflOOmg/kg body weight 30 minutes before exposure to 8CMKlradiation for 40 minutes. Parameters included the estimation of heart glycogen serum GOT, LDH and cholesterol and blood lactate and pyruvate. All the results showedsignificantinhibitory action againstradiation inducedchanges inthemyocardiumofrats.
513
THE PERIPHERALBLOOD MONOCYTE SUBPOPULATIONS IN NORMALSUBJECTSAND AND PATIENTS WITH ANGINA PECTOFUSo LKondakov, N,Menzianova, A,Kubatiev. Central, Institute of Postgraduate Medical Training, Moscow, USSR. Using immunocytochemzl.cel, cy-tochemical and electxon microscopic methods peripheral blood monocyte (Man) subpopulations were studied in normal subjects (N), 23 patients with stable angina pectoris (SA) in NYHA functional classes II, III and IV, and in 13 patients with progressive angina (PA). The proportion of lipid-bearing Mon was found to be in N no more than lo%&, while in SA patients it rose with the functional class end in class IV reached 36,6% In 3% cases th%s proportion exceeded 50$& The changes in Moo subpopulations associated with an increase in the severity of.angina pectoris suggest the existence in humans of a mechanism of Lipid clearance by Mono s.171
(Supplement
II) (1989)
S.A. Elgebaly, A. Cohen, M. Allam, N. Yekta. 51 1 CARDIAC DERIVED INFLAMMATORY MEDIATORS. Department of Surgery, University of Connecticut School of Medicine, Farmington, Connecticut, U.S.A. Recent studies from our laboratory have indicated the release of neutrophil chemotactic factors (NCF) from isolated rabbit hearts perfused with cardioplegia (Am Co11 Surg 38:276, 1987). These factors are protein in nature, heat unstable, not extractand exhibit a molecular weight able F organic solvents (i.e., not leukotrienes), value of greater than 100 Kd. The purpose of this study is to test the hypothesis that NCF released from isolated hearts will not only stimulate neutrophil recruitment Rabbit to the heart, but will also activate neutrophils to secrete lysosomal enzymes. peritoneal neutrophils were therefore incubated with the high molecular weight cardiac derived NCF for 5 minutes. Supernatant solutions were then removed and assayed for Results are expressed as percent of maximum the enzyme N-acetyl-B-glucosaminidase. We found that cardiac NCF stimulated the rerelease as determined by lysed calls. lease of 4053% of the enzyme. The standard chemotactic factor f-Met-Leu-Phe induced 4624%. The cytoplasmic enzyme lactic dehydrogenase was not liberated, indicating that the lysosomal enzyme was actively secreted and not released subsequent to cell lysis. These studies further support a role of ischemic myocardial tissues in cardiac inflammation by releasing inflammatory mediators which recruit neutrophils from the circulation to the heart and activate them to release damaging enzymes.
5 12 EFFECT OF THE AQUEOUS EXTRACT OF DELPHMIUM DENUDATUM WALL AGAINST RADIATION INDUCED CHANGES IN RATS MYOCARWJh4. Khan, A.B. A.K. Tibbiya College, A.M.U., Aligarh, India. Earlier studies in our laboratory showed evidence of dose related acute injury in the myocardium after exposure of rats to ionizing radiation. In further continuation of this study, the effects of intervention by pre-treatment with the aqueous extract of pelnhinium denudatum wall have been studies. The study was carried out in healthy albino rats ofeithersex weighing between 150-200 grams. Thetestgroup ofrats was treated with theaqueousextractof Delnhiniurn denudatumwtiora.lly inthedosesoflOOmg/kg body weight 30 minutes before exposure to 8CMKlradiation for 40 minutes. Parameters included the estimation of heart glycogen serum GOT, LDH and cholesterol and blood lactate and pyruvate. All the results showedsignificantinhibitory action againstradiation inducedchanges inthemyocardiumofrats.
513
THE PERIPHERALBLOOD MONOCYTE SUBPOPULATIONS IN NORMALSUBJECTSAND AND PATIENTS WITH ANGINA PECTOFUSo LKondakov, N,Menzianova, A,Kubatiev. Central, Institute of Postgraduate Medical Training, Moscow, USSR. Using immunocytochemzl.cel, cy-tochemical and electxon microscopic methods peripheral blood monocyte (Man) subpopulations were studied in normal subjects (N), 23 patients with stable angina pectoris (SA) in NYHA functional classes II, III and IV, and in 13 patients with progressive angina (PA). The proportion of lipid-bearing Mon was found to be in N no more than lo%&, while in SA patients it rose with the functional class end in class IV reached 36,6% In 3% cases th%s proportion exceeded 50$& The changes in Moo subpopulations associated with an increase in the severity of.angina pectoris suggest the existence in humans of a mechanism of Lipid clearance by Mono s.171