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s middle-aged patients with nonalcoholic fatty liver disease
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Abstracts / Atherosclerosis 252 (2016) e1ee196
Conclusions: Serum MCP-1levels was statistically significant higher in patients with NASH than in the control group and to this increase contributes both as visceral adipose tissue as hepatic inflammation. In the same time, in patients with NASH the liver may be as well a targhet and a source of some pro-inflammatory mediators. Keywords: non-alcoholic steatohepatitis, monocyte chemoattractant protein-1, visceral fat thickness, carotid intima- media thickness, hepatic inflammation
EAS16-0580, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: NASH AND LIVER ABNORMALITIES. CARDIOMETABOLIC RISK FACTORS IN YOUNG V/S MIDDLE-AGED PATIENTS WITH NONALCOHOLIC FATTY LIVER DISEASE
fibrillar collagen and fat. We also developed a method for quantitative assessment of early fibrosis using automated analysis of SHG signals. Results: We found that the SHG mean signal intensity correlated well with fibrosis stage and the mean CARS signal intensity with liver fat. Little overlap in SHG signal intensities between fibrosis stages 0 and 1 was observed. A specific fibrillar SHG signal was detected in the liver parenchyma outside portal areas in all samples histologically classified as having no fibrosis. This signal correlated with immunohistochemical location of fibrillar collagens I and III. Conclusions: This study provides evidence that label-free SHG imaging detects fibrillar collagen deposition in NAFLD more sensitively than routine histological staging. It also enables observer-independent quantification of early fibrosis in NAFLD with continuous grading.
R. Ivanova 1, C. Marinova 2, A. Alexiev 2, L. Mateva 2. 1 University Hospital Alexandrovska- Medical University of Sofia, Clinic of Cardiology, Sofia, Bulgaria; 2 University Hospital St. Ivan Rilski- Medical University of Sofia, Clinic of Gastroenterology, Sofia, Bulgaria
EAS16-0703, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: NASH AND LIVER ABNORMALITIES. THE ROLE OF VITAMIN D3 IN HIGH FAT DIET INDUCED FATTY LIVER DISEASE AND OBESITY
Objectives: Metabolic changes associated with increased cardiovascular risk (CVR) in nonalcoholic fatty liver disease (NAFLD) are present increasingly in young men. We aimed to compare the frequency and the severity of metabolic changes in young and middle-aged patients with NAFLD.
R. Kheder 1, J. Hobkir 2, M. Browning 3, C. Stover 3. 1 University of Leicester, Department of Infection- Immunity and Inflammation, Leicester, United Kingdom; 2 university of Hull, Department of Sport- Health and Exercise Scienc, Hull, United Kingdom; 3 University of Leicester, Infection- Immunity and Inflammation, Leicester, United Kingdom
Methods: In this study were included 215 NAFLD cases in young age (< 45 y) and 188 cases in middle age (> 45 y). The following metabolic parameters were compared: BMI, features of metabolic syndrome (MS), lipids, glucose, insulin and HOMA-IR. Results: The portions of cases with obesity and overweight, MS, abdominal obesity, arterial hypertension, decreased HDL-cholesterol, increased triglycerides and total cholesterol, diabetes type 2, HOMA-IR >2 and insulin >100 mU/l during OGTT were equal between the both aged groups. In middle-aged patients compared to young cases, the frequency of increased LDL-cholesterol and the mean level were higher (р¼0.004 and р¼0.029), glucose > 5.6 mmol/l (p¼0.01) and the mean glucose levels during OGTT were also higher (р¼0.039), as well as the mean levels of systolic and diastolic blood pressure (р¼0.01 and р¼0.033). Correlations of these parameters with the age were also found (р¼0.020-0.001). In evaluation of sex differences, we found that the portion of males in the young-aged group was significantly larger (p¼0.0001). Conclusions: There were no differences in the portions of deviations and the mean levels of the most metabolic parameters between the young and middle-aged NAFLD patients. This confirms that cardiometabolic risk factors in NAFLD are present even in young age.
Objectives: Hepatocyte lipid accumulation is characteristic of non-alcoholic fatty liver disease (NAFLD). This may lead to hepatic inflammation; which is called non-alcoholic steatohepatitis (NASH) and fibrosis. This study investigates the role of vitamin D3 in diet-induced obesity in mice
EAS16-0918, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: NASH AND LIVER ABNORMALITIES. CONTINUOUS GRADING OF EARLY FIBROSIS IN NAFLD USING LABELFREE IMAGING €devirta 3, P. Luukkonen 3, S.M. Karppinen 4, T. J. Pirhonen 1, J. Arola 2, S. Sa €ki 1, M. Hukkanen 1, H. Yki-Ja €rvinen 3, E. Pihlajaniemi 4, A. Isoma Ikonen 1. 1 University of Helsinki, Department of Anatomy, Helsinki, Finland; 2 University of Helsinki, Department of Pathology, Helsinki, Finland; 3 University of Helsinki, Department of Medicine, Helsinki, Finland; 4 University of Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu, Finland Objectives: Early detection of fibrosis is important in identifying individuals at risk for advanced liver disease in non-alcoholic fatty liver disease (NAFLD). In this study, we assessed whether second-harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy, detecting fibrillar collagen and fat in a label-free manner, might allow automated and sensitive quantification of early fibrosis in NAFLD. Methods: We analyzed 32 surgical biopsies from patients covering histological fibrosis stages 0-4, using multimodal label-free microscopy. Native samples were visualized by SHG and CARS imaging for detecting
Methods: LDLR-/-, LDLR+/+ mice. High fat high sucrose diet formulation led to the development steatosis (10- weeks) in the experimental animals with variation in severity and variable inflammation. Body weight, fat pad weight, liver histology were analysed. Hepatic expression of candidate genes (TNF-a, srebp-1c, TLR4, HMGCR, SR-B1, PPAR-g) was performed by qPCR. ELISA was used to quantify serum insulin, Adiponectin, MDA, and IL6, AST, ALT and endotoxin levels were measured. Results: High fat high sucrose diet led to an increase of body weight, histological features of fatty liver and liver inflammation, insulin resistance, MDA elevation, altered liver function tests and elevated IL-6. High fat high sucrose diet with supplemented Vitamin D led to findings comparable with the control group that was analysed in parallel (low fat maintenance diet). Conclusions: High fat high sucrose diet led to the development NAFLD and insulin resistance, and this was ameliorated when Vitamin D was present in the high fat high sucrose diet.
EAS16-0615, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: NASH AND LIVER ABNORMALITIES. COMPARATIVE ANALYSIS OF SERUM LIPIDS IN PATIENTS WITH CHRONIC HEPATITIS C, NONALCOHOLIC FATTY LIVER DISEASE, AND HEALTHY CONTROLS R. Ivanova 1, I. Valkov 2, C. Marinova 2, A. Alexiev 2, K. Antonov 2, D. Jelev 2, L. Mateva 2. 1 University Hospital Alexandrovska- Medical University of Sofia, Clinic of Cardiology, Sofia, Bulgaria; 2 University Hospital St. Ivan Rilski- Medical University of Sofia, Clinic of Gastroenterology, Sofia, Bulgaria Objectives: Serum lipids abnormalities are usually seen in patients with chronic hepatitis C (CHC), but the impact of concomitant steatosis, as well as differences with nonalcoholic fatty liver disease (NAFLD) are not well established yet. The aim of the study was to assess and compare the serum lipids in patients with CHC genotype 1, those with NAFLD, and healthy controls (HC). Methods: A total of 1010 subjects were included in this study: 366 CHC genotype 1 patients with (n¼227) or without (n¼139) steatosis, 403 NAFLD patients, and 241 HC without liver or other diseases, matched for age and gender. Serum lipids, BMI, components of metabolic syndrome
Abstracts / Atherosclerosis 252 (2016) e1ee196
Conclusions: Serum MCP-1levels was statistically significant higher in patients with NASH than in the control group and to this increase contributes both as visceral adipose tissue as hepatic inflammation. In the same time, in patients with NASH the liver may be as well a targhet and a source of some pro-inflammatory mediators. Keywords: non-alcoholic steatohepatitis, monocyte chemoattractant protein-1, visceral fat thickness, carotid intima- media thickness, hepatic inflammation
EAS16-0580, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: NASH AND LIVER ABNORMALITIES. CARDIOMETABOLIC RISK FACTORS IN YOUNG V/S MIDDLE-AGED PATIENTS WITH NONALCOHOLIC FATTY LIVER DISEASE
fibrillar collagen and fat. We also developed a method for quantitative assessment of early fibrosis using automated analysis of SHG signals. Results: We found that the SHG mean signal intensity correlated well with fibrosis stage and the mean CARS signal intensity with liver fat. Little overlap in SHG signal intensities between fibrosis stages 0 and 1 was observed. A specific fibrillar SHG signal was detected in the liver parenchyma outside portal areas in all samples histologically classified as having no fibrosis. This signal correlated with immunohistochemical location of fibrillar collagens I and III. Conclusions: This study provides evidence that label-free SHG imaging detects fibrillar collagen deposition in NAFLD more sensitively than routine histological staging. It also enables observer-independent quantification of early fibrosis in NAFLD with continuous grading.
R. Ivanova 1, C. Marinova 2, A. Alexiev 2, L. Mateva 2. 1 University Hospital Alexandrovska- Medical University of Sofia, Clinic of Cardiology, Sofia, Bulgaria; 2 University Hospital St. Ivan Rilski- Medical University of Sofia, Clinic of Gastroenterology, Sofia, Bulgaria
EAS16-0703, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: NASH AND LIVER ABNORMALITIES. THE ROLE OF VITAMIN D3 IN HIGH FAT DIET INDUCED FATTY LIVER DISEASE AND OBESITY
Objectives: Metabolic changes associated with increased cardiovascular risk (CVR) in nonalcoholic fatty liver disease (NAFLD) are present increasingly in young men. We aimed to compare the frequency and the severity of metabolic changes in young and middle-aged patients with NAFLD.
R. Kheder 1, J. Hobkir 2, M. Browning 3, C. Stover 3. 1 University of Leicester, Department of Infection- Immunity and Inflammation, Leicester, United Kingdom; 2 university of Hull, Department of Sport- Health and Exercise Scienc, Hull, United Kingdom; 3 University of Leicester, Infection- Immunity and Inflammation, Leicester, United Kingdom
Methods: In this study were included 215 NAFLD cases in young age (< 45 y) and 188 cases in middle age (> 45 y). The following metabolic parameters were compared: BMI, features of metabolic syndrome (MS), lipids, glucose, insulin and HOMA-IR. Results: The portions of cases with obesity and overweight, MS, abdominal obesity, arterial hypertension, decreased HDL-cholesterol, increased triglycerides and total cholesterol, diabetes type 2, HOMA-IR >2 and insulin >100 mU/l during OGTT were equal between the both aged groups. In middle-aged patients compared to young cases, the frequency of increased LDL-cholesterol and the mean level were higher (р¼0.004 and р¼0.029), glucose > 5.6 mmol/l (p¼0.01) and the mean glucose levels during OGTT were also higher (р¼0.039), as well as the mean levels of systolic and diastolic blood pressure (р¼0.01 and р¼0.033). Correlations of these parameters with the age were also found (р¼0.020-0.001). In evaluation of sex differences, we found that the portion of males in the young-aged group was significantly larger (p¼0.0001). Conclusions: There were no differences in the portions of deviations and the mean levels of the most metabolic parameters between the young and middle-aged NAFLD patients. This confirms that cardiometabolic risk factors in NAFLD are present even in young age.
Objectives: Hepatocyte lipid accumulation is characteristic of non-alcoholic fatty liver disease (NAFLD). This may lead to hepatic inflammation; which is called non-alcoholic steatohepatitis (NASH) and fibrosis. This study investigates the role of vitamin D3 in diet-induced obesity in mice
EAS16-0918, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: NASH AND LIVER ABNORMALITIES. CONTINUOUS GRADING OF EARLY FIBROSIS IN NAFLD USING LABELFREE IMAGING €devirta 3, P. Luukkonen 3, S.M. Karppinen 4, T. J. Pirhonen 1, J. Arola 2, S. Sa €ki 1, M. Hukkanen 1, H. Yki-Ja €rvinen 3, E. Pihlajaniemi 4, A. Isoma Ikonen 1. 1 University of Helsinki, Department of Anatomy, Helsinki, Finland; 2 University of Helsinki, Department of Pathology, Helsinki, Finland; 3 University of Helsinki, Department of Medicine, Helsinki, Finland; 4 University of Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu, Finland Objectives: Early detection of fibrosis is important in identifying individuals at risk for advanced liver disease in non-alcoholic fatty liver disease (NAFLD). In this study, we assessed whether second-harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy, detecting fibrillar collagen and fat in a label-free manner, might allow automated and sensitive quantification of early fibrosis in NAFLD. Methods: We analyzed 32 surgical biopsies from patients covering histological fibrosis stages 0-4, using multimodal label-free microscopy. Native samples were visualized by SHG and CARS imaging for detecting
Methods: LDLR-/-, LDLR+/+ mice. High fat high sucrose diet formulation led to the development steatosis (10- weeks) in the experimental animals with variation in severity and variable inflammation. Body weight, fat pad weight, liver histology were analysed. Hepatic expression of candidate genes (TNF-a, srebp-1c, TLR4, HMGCR, SR-B1, PPAR-g) was performed by qPCR. ELISA was used to quantify serum insulin, Adiponectin, MDA, and IL6, AST, ALT and endotoxin levels were measured. Results: High fat high sucrose diet led to an increase of body weight, histological features of fatty liver and liver inflammation, insulin resistance, MDA elevation, altered liver function tests and elevated IL-6. High fat high sucrose diet with supplemented Vitamin D led to findings comparable with the control group that was analysed in parallel (low fat maintenance diet). Conclusions: High fat high sucrose diet led to the development NAFLD and insulin resistance, and this was ameliorated when Vitamin D was present in the high fat high sucrose diet.
EAS16-0615, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: NASH AND LIVER ABNORMALITIES. COMPARATIVE ANALYSIS OF SERUM LIPIDS IN PATIENTS WITH CHRONIC HEPATITIS C, NONALCOHOLIC FATTY LIVER DISEASE, AND HEALTHY CONTROLS R. Ivanova 1, I. Valkov 2, C. Marinova 2, A. Alexiev 2, K. Antonov 2, D. Jelev 2, L. Mateva 2. 1 University Hospital Alexandrovska- Medical University of Sofia, Clinic of Cardiology, Sofia, Bulgaria; 2 University Hospital St. Ivan Rilski- Medical University of Sofia, Clinic of Gastroenterology, Sofia, Bulgaria Objectives: Serum lipids abnormalities are usually seen in patients with chronic hepatitis C (CHC), but the impact of concomitant steatosis, as well as differences with nonalcoholic fatty liver disease (NAFLD) are not well established yet. The aim of the study was to assess and compare the serum lipids in patients with CHC genotype 1, those with NAFLD, and healthy controls (HC). Methods: A total of 1010 subjects were included in this study: 366 CHC genotype 1 patients with (n¼227) or without (n¼139) steatosis, 403 NAFLD patients, and 241 HC without liver or other diseases, matched for age and gender. Serum lipids, BMI, components of metabolic syndrome