- Email: [email protected]
Cell-to-cell contact and extracellular matrix
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Cell-to-cell contact and extracellular matrix Web alert Jonathon Pines*, Luca Toldo† and Frank Lafont‡ Addresses *Wellcome/CRC Institute, Tennis Court Road, Cambridge, CB2 1QR, UK; e-mail: [email protected] † MERCK KGaA, Bio- and ChemoInformatics, 64271 Darmstadt, Germany; e-mail: [email protected] ‡ Cell Biology Programme, European Molecular Biology Laboratory. Meyerhofstrasse 1, Heidelberg 69012, Germany; e-mail: [email protected]
neural crests and an animation of neural crest cell migration in Xenopus. Other pages describe a project concerning the role of the intermediate filament protein desmin in cardiomyocyte cell–cell adhesion. Some methods related to whole-mount immunocytochemistry and in situ hybridisation are detailed (http://spot.colorado.edu/~klym/methods.html).
Current Opinion in Cell Biology 1999, 11:535–536
Cell adhesion and cytoskeleton
Adhesion molecules and neurons L1CAM Mutation Web Page http://dnalab-www.uia.ac.be/dnalab/l1/ Everything you wanted to know about the adhesion molecule L1CAM, which plays an important role in the development of the nervous system, with an impressive table of known mutations. The site has clickable links to other databases such as OMIM (on-line Mendelian inheritance in man). Movies from Vance’s Lab http://neurowww.cwru.edu/Lemmon/ The Vance laboratory focuses on the L1 adhesion molecule and its influence on axon growth. This site presents some MPEG movies of growth cone migration on different extracellular matrix-coated substrata. Neurons and their Growth Cones http://gramercy.ios.com/~pab9/index.html This Website is maintained by Perry A Brittis and presents some movies of growth cone behaviour when incubated with different adhesion molecules. It gives basic information on cell adhesion molecules together with a description of the methods used for imaging — for instance how to prepare samples for video microscopy recording — and some technical procedures on time lapse imaging and images analysis.
Cadherins Cadherins from the FlyBase http://sdb.bio.purdue.edu/fly/aimain/6transm.htm#dafka2 This site provides a list of the Drosophila cadherin homologs with information summarised according to biological functions, protein structure, evolutionary homologs, regulation, and developmental biology topics. It also includes a list of references. Drosophila Cadherins http://mcb1.ims.abdn.ac.uk/Jpet/droscad.htm This page from J Pettitt provides an alternative entry to the Drosophila cadherins from the Flybase DataBase. Data about the cadherins in C. elegans can be obtained (http://mcb1.ims.abdn.ac.uk/Jpet/cadA.htm). Klymkowsky Laboratory Homepage http://spot.colorado.edu/~klym/ This page is focused on adhesion molecules (mainly catenins) on neural crest induction and patterning, and on heart development and disease. The site includes an introduction to
E Bonder Homepage http://biology-newark.rutgers.edu/cellbio/default.htm Movies from recently published articles (but also unpublished material from E Bonder and colleagues) showing actin re-organisation during cell motility and establishment of cell–cell contact in epithelial cells (http://biology-newark. rutgers.edu/cellbio/mirapg.htm ).
Fibronectin extracellular matrix assembly Time-lapse Confocal Reflection Imaging of ECM Assembly in vitro http://www.cyto.purdue.edu/flowcyt/research/imaging/tlrecm.htm This site has several quicktime movies (0.5–2.5 Mbyte) using time-lapse reflection imaging to visualise the assembly of a variety of extracellular matrices in vitro. These include the assembly of collagen and GAGs and pepsin-solubilised intestinal submucosal. Time lapse reflection imaging uses laser light to illuminate the sample but only light reflected by the polymer is collected and imaged.
Integrins The Integrin Page http://www.geocities.com/CapeCanaveral/9629/ or http://integrins.hypermart.net (for JAVA enabled browser) This site provides basic information on integrins and their subunits. There is a useful table of the known αβ combinations and their ligands, a link to the online ‘integrin questions and answers page’ and some information on some other extracellular matrix molecules. Horwitz’s Laboratory Homepage http://www.people.virginia.edu/~afh2n/default.html This home page describes the Horwitz lab research on integrins and several nice movies and three-dimensional images related to these molecules. Two of the movies are over 30 Mbytes and take a while to download.
Dystroglycan and proteoglycans Dystroglycan Page http://www.dmd.nl/dg_home.html Dystroglycan-associated protein page from the Leiden Muscular Dystrophy Website giving basic information about dystroglycan and a list (with references) of antibodies against http://www.dmd.nl/antibosg.html.
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Dystroglycan Expression During Kidney Development http://www.ana.ed.ac.uk/anatomy/database/kidbase/ecmk.html Table from the Kidney Development Database showing the expression pattern during kidney development of extracellular matix molecules and their receptors including dystroglycan. This was last updated in June 1997. Glycoforum on Proteoglycans http://www.glycoforum.gr.jp/science/word/proteoglycan/ PG_E.html This page offers a comprehensive introduction (in English or Japanese) to several proteoglycans and glycosaminoglycans. It includes information on the role of proteoglycans in brain development, the basement membrane, signal transduction by syndecans and on heparin-binding proteins. Conferences on proteoglycans are referenced on the home page http://www.glycoforum.gr.jp/index.html .
Protein Complexes in Skeletal Muscle Fibers and Connective Tissue http://www.neuro.wustl.edu/neuromuscular/musdist/dag2.htm #ad This page from the Neuromuscular homepage gives clickable maps of the molecular components engaged in extrajunctional muscle and neuromuscular junctions. Direct links to the SWISS-PROT database, and to the OMIM database are provided (e.g. concerning the α- and β-dystroglycans). Proteoglycans and Glycosaminoglycans Page http://bssv01.lancs.ac.uk/gig/pages/toppage.htm This Website gives a general introduction to and the nomenclature of the different glycosaminoglycans and proteoglycan types. Although not all of the links are still active (the page appears to have been constructed in 1997) there are useful links to other carbohydrate-related sites and newsgroups.
10/27/1999 7:52 AM
Page 535
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Cell-to-cell contact and extracellular matrix Web alert Jonathon Pines*, Luca Toldo† and Frank Lafont‡ Addresses *Wellcome/CRC Institute, Tennis Court Road, Cambridge, CB2 1QR, UK; e-mail: [email protected] † MERCK KGaA, Bio- and ChemoInformatics, 64271 Darmstadt, Germany; e-mail: [email protected] ‡ Cell Biology Programme, European Molecular Biology Laboratory. Meyerhofstrasse 1, Heidelberg 69012, Germany; e-mail: [email protected]
neural crests and an animation of neural crest cell migration in Xenopus. Other pages describe a project concerning the role of the intermediate filament protein desmin in cardiomyocyte cell–cell adhesion. Some methods related to whole-mount immunocytochemistry and in situ hybridisation are detailed (http://spot.colorado.edu/~klym/methods.html).
Current Opinion in Cell Biology 1999, 11:535–536
Cell adhesion and cytoskeleton
Adhesion molecules and neurons L1CAM Mutation Web Page http://dnalab-www.uia.ac.be/dnalab/l1/ Everything you wanted to know about the adhesion molecule L1CAM, which plays an important role in the development of the nervous system, with an impressive table of known mutations. The site has clickable links to other databases such as OMIM (on-line Mendelian inheritance in man). Movies from Vance’s Lab http://neurowww.cwru.edu/Lemmon/ The Vance laboratory focuses on the L1 adhesion molecule and its influence on axon growth. This site presents some MPEG movies of growth cone migration on different extracellular matrix-coated substrata. Neurons and their Growth Cones http://gramercy.ios.com/~pab9/index.html This Website is maintained by Perry A Brittis and presents some movies of growth cone behaviour when incubated with different adhesion molecules. It gives basic information on cell adhesion molecules together with a description of the methods used for imaging — for instance how to prepare samples for video microscopy recording — and some technical procedures on time lapse imaging and images analysis.
Cadherins Cadherins from the FlyBase http://sdb.bio.purdue.edu/fly/aimain/6transm.htm#dafka2 This site provides a list of the Drosophila cadherin homologs with information summarised according to biological functions, protein structure, evolutionary homologs, regulation, and developmental biology topics. It also includes a list of references. Drosophila Cadherins http://mcb1.ims.abdn.ac.uk/Jpet/droscad.htm This page from J Pettitt provides an alternative entry to the Drosophila cadherins from the Flybase DataBase. Data about the cadherins in C. elegans can be obtained (http://mcb1.ims.abdn.ac.uk/Jpet/cadA.htm). Klymkowsky Laboratory Homepage http://spot.colorado.edu/~klym/ This page is focused on adhesion molecules (mainly catenins) on neural crest induction and patterning, and on heart development and disease. The site includes an introduction to
E Bonder Homepage http://biology-newark.rutgers.edu/cellbio/default.htm Movies from recently published articles (but also unpublished material from E Bonder and colleagues) showing actin re-organisation during cell motility and establishment of cell–cell contact in epithelial cells (http://biology-newark. rutgers.edu/cellbio/mirapg.htm ).
Fibronectin extracellular matrix assembly Time-lapse Confocal Reflection Imaging of ECM Assembly in vitro http://www.cyto.purdue.edu/flowcyt/research/imaging/tlrecm.htm This site has several quicktime movies (0.5–2.5 Mbyte) using time-lapse reflection imaging to visualise the assembly of a variety of extracellular matrices in vitro. These include the assembly of collagen and GAGs and pepsin-solubilised intestinal submucosal. Time lapse reflection imaging uses laser light to illuminate the sample but only light reflected by the polymer is collected and imaged.
Integrins The Integrin Page http://www.geocities.com/CapeCanaveral/9629/ or http://integrins.hypermart.net (for JAVA enabled browser) This site provides basic information on integrins and their subunits. There is a useful table of the known αβ combinations and their ligands, a link to the online ‘integrin questions and answers page’ and some information on some other extracellular matrix molecules. Horwitz’s Laboratory Homepage http://www.people.virginia.edu/~afh2n/default.html This home page describes the Horwitz lab research on integrins and several nice movies and three-dimensional images related to these molecules. Two of the movies are over 30 Mbytes and take a while to download.
Dystroglycan and proteoglycans Dystroglycan Page http://www.dmd.nl/dg_home.html Dystroglycan-associated protein page from the Leiden Muscular Dystrophy Website giving basic information about dystroglycan and a list (with references) of antibodies against http://www.dmd.nl/antibosg.html.
cbb5web.qxd
10/27/1999 7:52 AM
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Web alert
Dystroglycan Expression During Kidney Development http://www.ana.ed.ac.uk/anatomy/database/kidbase/ecmk.html Table from the Kidney Development Database showing the expression pattern during kidney development of extracellular matix molecules and their receptors including dystroglycan. This was last updated in June 1997. Glycoforum on Proteoglycans http://www.glycoforum.gr.jp/science/word/proteoglycan/ PG_E.html This page offers a comprehensive introduction (in English or Japanese) to several proteoglycans and glycosaminoglycans. It includes information on the role of proteoglycans in brain development, the basement membrane, signal transduction by syndecans and on heparin-binding proteins. Conferences on proteoglycans are referenced on the home page http://www.glycoforum.gr.jp/index.html .
Protein Complexes in Skeletal Muscle Fibers and Connective Tissue http://www.neuro.wustl.edu/neuromuscular/musdist/dag2.htm #ad This page from the Neuromuscular homepage gives clickable maps of the molecular components engaged in extrajunctional muscle and neuromuscular junctions. Direct links to the SWISS-PROT database, and to the OMIM database are provided (e.g. concerning the α- and β-dystroglycans). Proteoglycans and Glycosaminoglycans Page http://bssv01.lancs.ac.uk/gig/pages/toppage.htm This Website gives a general introduction to and the nomenclature of the different glycosaminoglycans and proteoglycan types. Although not all of the links are still active (the page appears to have been constructed in 1997) there are useful links to other carbohydrate-related sites and newsgroups.