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Cerebellar projections from the lateral suprasylvian visual area in the cat
$71 C E R E B E L L A R C O R T I C O - N U C L E O - C O R T I C A L AND C O M M I S S U R A L WITH THE M U L T I P L E F L U O R E S C E N T T R A C I N G M E T H O D
NUCLEAR
CONNECTIONS
S H I G E Y U K I DEURA, K A Z U K O HASHIMOTO* and IORI YAMADA*, D e p a r t m e n t U n i v e r s i t y School of Medicine, T s u k a s a - m a c h i , Gifu 500, Japan. Our p r e v i o u s short-
anatomical
and l o n g - d i s t a n c e
istence
of the c o r t i c o - n u c l e o - c o r t i c a l
physiologically. intrinsic method. were
and p h y s i o l o g i c a l
cortico-cortical
This
connections In the
injected
first
were
found
as in the i p s i l a t e r a l
was used
lateral
lobule(PM).
posterior
NOBU0 Inst.
interpositus
were
cerebellar
indicated
different
labeled
which
to forelimb
the same
to the
into either
cerebellar
some of the PIN n e u r o n s
tracers lobe(ANT)
cells
in
areas
technique
forelimb
send c o l l a t e r a l s
of tracers
PIN or superior
fluorescent
in the ANT as well
branches
project also
tracing
Some P u r k i n j e
set of experiments,
peduncle
electro-
of the c e r e b e l l a r
by one or both of
to the injection nucleus(PIN).
are
The ex-
of the a n t e r i o r
cells
Gifu
that there
fluorescent
areas
to send axonal
application
show that
interpositus
PROJECTIONS
found
the P I N neurons
and c o n t r a l a t e r a l
The data
adjacent
In the second
PIN by the c o m b i n e d
the c o n t r a l a t e r a l CEREBELLAR
Retrogradely
in the folia
to check w h e t h e r
PIN.
also
spectrally forelimb
revealed
details
set of experiments,
and of the PM.
area of the ANT
were
at i n v e s t i g a t i n g
defined
of Anatomy,
in the cerebellum.
of the m u l t i p l e
the A N T or to the superior tralateral
have
in the cat by m e a n s
the ANT and the PIN n e u r o n s of the A N T
connections
aimed
into p h y s i o l o g i c a l l y
and of the p a r a m e d i a n the tracers
s t u d ~ was
studies
connections
STUDIED
area of
to the con-
the forelimb
peduncle
and contra-
send c o m m i s s u r a l
axons
to
nucleus.
FROM THE LATERAL
SUPRASYLVIAN
KAT0*, SABUR0 K A W A G U C H I , A N D HIROFUMI MIYATA~ Brain Res., Fac. Med., K y o t o Univ., 606 KYOT0,
VISUAL
AREA
IN T H E CAT.
Japan
The medial wall of the lateral suprasylvian visual a r e a (mLS) in the cat, one of the cortical targets of the cerebellar projections, was f o u n d to project b a c k onto the cerebellar cortex, by e l e c t r o p h y s i o l o g i c a l r e c o r d i n g and the HRP method. R e s p o n s e s on s t i m u l a t i o n of the mLS were recorded from the cerebellar cortex, and c o m p a r e d w i t h those e v o k e d by s t i m u l a t i o n of the motor c o r t e x and the crown part of the parietal a s s o c i a t i o n cortex. The m L S - i n d u c e d r e s p o n s e s c o n s i s t e d of e a r l y m o s s y and late c l i m b i n g fibre responses. The m o s s y fibre response was evoked, at a latency of 2-3 ms, p r e d o m i n a n t l y in the lateral part of the c o n t r a l a t e r a l c e r e b e l l a r cortex (crus I, crus II, dorsal p a r a f l o c c u l u s and p a r a m e d i a n lobule) and the p o s t e r i o r part of the v e r m i s (lobuli VII and VIII). H R P study suggested that this response is t r a n s m i t t e d by the p o n t i n e gray. A f t e r H R P injections into mLS and into one of the c e r e b e l l a r r e s p o n s i v e areas, terminals of the c o r t i c o p o n t i n e projection and n e u r o n e s of o r i g i n of the p o n t o c e r e b e l l a r p r o j e c t i o n were labelled in the gray, where d i s t r i b u t i o n s of the two kinds of l a b e l l i n g overlapped. The climbing fibre response was elicited, at a latency of 17-19 ms, over wide area i n c l u d i n g b o t h the lateral and m e d i a l part of the cortex. This r e s p o n s e was unstable as c o m p a r e d w i t h the p r e c e d i n g m o s s y fibre response and was e l i c i t e d at one and a h a l f times as long a l a t e n c y as the motor c o r t e x - i n d u c e d c l i m b i n g fibre response. A d d i t i o n a l e x p e r i m e n t s w e r e made in cats whose crown part of the parietal a s s o c i a t i o n cortex was a b l a t e d more than 2 weeks before, in an attempt to exclude the slight p o s s i b i l i t y that the electric volley to the mLS m i g h t stimulate fibres o r i g i n a t i n g from the crown part. The m L S - i n d u c e d r e s p o n s e s were recorded and c o m p a r e d w i t h the responses in intact cats. The results from the o p e r a t e d cats were l a r g e l y similar to those o b t a i n e d f r o m intact cats: in b o t h groups of cats, the m L S - i n d u c e d mossy fibre response p r e v a i l e d in the lateral and p o s t e r i o r parts of the c e r e b e l l a r cortex w i t h a p r e d o m i n a n c e of the motor c o r t e x - i n d u c e d response in the medial and anterior parts.
NUCLEAR
CONNECTIONS
S H I G E Y U K I DEURA, K A Z U K O HASHIMOTO* and IORI YAMADA*, D e p a r t m e n t U n i v e r s i t y School of Medicine, T s u k a s a - m a c h i , Gifu 500, Japan. Our p r e v i o u s short-
anatomical
and l o n g - d i s t a n c e
istence
of the c o r t i c o - n u c l e o - c o r t i c a l
physiologically. intrinsic method. were
and p h y s i o l o g i c a l
cortico-cortical
This
connections In the
injected
first
were
found
as in the i p s i l a t e r a l
was used
lateral
lobule(PM).
posterior
NOBU0 Inst.
interpositus
were
cerebellar
indicated
different
labeled
which
to forelimb
the same
to the
into either
cerebellar
some of the PIN n e u r o n s
tracers lobe(ANT)
cells
in
areas
technique
forelimb
send c o l l a t e r a l s
of tracers
PIN or superior
fluorescent
in the ANT as well
branches
project also
tracing
Some P u r k i n j e
set of experiments,
peduncle
electro-
of the c e r e b e l l a r
by one or both of
to the injection nucleus(PIN).
are
The ex-
of the a n t e r i o r
cells
Gifu
that there
fluorescent
areas
to send axonal
application
show that
interpositus
PROJECTIONS
found
the P I N neurons
and c o n t r a l a t e r a l
The data
adjacent
In the second
PIN by the c o m b i n e d
the c o n t r a l a t e r a l CEREBELLAR
Retrogradely
in the folia
to check w h e t h e r
PIN.
also
spectrally forelimb
revealed
details
set of experiments,
and of the PM.
area of the ANT
were
at i n v e s t i g a t i n g
defined
of Anatomy,
in the cerebellum.
of the m u l t i p l e
the A N T or to the superior tralateral
have
in the cat by m e a n s
the ANT and the PIN n e u r o n s of the A N T
connections
aimed
into p h y s i o l o g i c a l l y
and of the p a r a m e d i a n the tracers
s t u d ~ was
studies
connections
STUDIED
area of
to the con-
the forelimb
peduncle
and contra-
send c o m m i s s u r a l
axons
to
nucleus.
FROM THE LATERAL
SUPRASYLVIAN
KAT0*, SABUR0 K A W A G U C H I , A N D HIROFUMI MIYATA~ Brain Res., Fac. Med., K y o t o Univ., 606 KYOT0,
VISUAL
AREA
IN T H E CAT.
Japan
The medial wall of the lateral suprasylvian visual a r e a (mLS) in the cat, one of the cortical targets of the cerebellar projections, was f o u n d to project b a c k onto the cerebellar cortex, by e l e c t r o p h y s i o l o g i c a l r e c o r d i n g and the HRP method. R e s p o n s e s on s t i m u l a t i o n of the mLS were recorded from the cerebellar cortex, and c o m p a r e d w i t h those e v o k e d by s t i m u l a t i o n of the motor c o r t e x and the crown part of the parietal a s s o c i a t i o n cortex. The m L S - i n d u c e d r e s p o n s e s c o n s i s t e d of e a r l y m o s s y and late c l i m b i n g fibre responses. The m o s s y fibre response was evoked, at a latency of 2-3 ms, p r e d o m i n a n t l y in the lateral part of the c o n t r a l a t e r a l c e r e b e l l a r cortex (crus I, crus II, dorsal p a r a f l o c c u l u s and p a r a m e d i a n lobule) and the p o s t e r i o r part of the v e r m i s (lobuli VII and VIII). H R P study suggested that this response is t r a n s m i t t e d by the p o n t i n e gray. A f t e r H R P injections into mLS and into one of the c e r e b e l l a r r e s p o n s i v e areas, terminals of the c o r t i c o p o n t i n e projection and n e u r o n e s of o r i g i n of the p o n t o c e r e b e l l a r p r o j e c t i o n were labelled in the gray, where d i s t r i b u t i o n s of the two kinds of l a b e l l i n g overlapped. The climbing fibre response was elicited, at a latency of 17-19 ms, over wide area i n c l u d i n g b o t h the lateral and m e d i a l part of the cortex. This r e s p o n s e was unstable as c o m p a r e d w i t h the p r e c e d i n g m o s s y fibre response and was e l i c i t e d at one and a h a l f times as long a l a t e n c y as the motor c o r t e x - i n d u c e d c l i m b i n g fibre response. A d d i t i o n a l e x p e r i m e n t s w e r e made in cats whose crown part of the parietal a s s o c i a t i o n cortex was a b l a t e d more than 2 weeks before, in an attempt to exclude the slight p o s s i b i l i t y that the electric volley to the mLS m i g h t stimulate fibres o r i g i n a t i n g from the crown part. The m L S - i n d u c e d r e s p o n s e s were recorded and c o m p a r e d w i t h the responses in intact cats. The results from the o p e r a t e d cats were l a r g e l y similar to those o b t a i n e d f r o m intact cats: in b o t h groups of cats, the m L S - i n d u c e d mossy fibre response p r e v a i l e d in the lateral and p o s t e r i o r parts of the c e r e b e l l a r cortex w i t h a p r e d o m i n a n c e of the motor c o r t e x - i n d u c e d response in the medial and anterior parts.