Cerebrospinal fluid lymphocytes new function is a neurotransmitters synthesis

208 CEREBROSPINAL FLUID LYMPHOCYTES N E W FUNCTION IS A NEUROTRANSMITTERS SYNTHESIS R. Sepiashvili, Y. M a l a s h k h i a Institute of Clinical Immunology mud Allergology Tskhaltubo, Georgia, USSR Cerebrospinal fluid (CSF) iymphocytes have been cuimred in RPMI-1640 for 7 days. Acetylcholines and serotonius concentration in CSF and supematant fluid of lymphocytes culture have been studied by chromatography. Acetylcholines concentration in CSF has been counted to be 0,11-+0,03 mkg/ml, in supematant fluid - 0,035-+0,002 mkg/ml. Serotonins concentration has been in CSF - 42*_2pg/ml, and in supematant fluid 28+-2 pg/ml. So, on the basis of the data obtained one can conclude neurotransmitters are induced both by central nervous and immune systems. From the above mentioned acetylcholine and serotonin can be termed both as neuro- and immunotrasmitters. The exchanges in the acetylcholines and serotonins synthesis by the lymphocytes can induce brain disfunctinn evolving memory disturbances. Results showed that the patients with memory disturbances have evident decrease of B-endorphine and vasopressin concentrations in CSF, blood and supernatant fluid, proving the possibility of synthesis of the above substances by the CSF and blood lymphocytes and allowing to assume the participation of immunomodulators and mediators generated by lymphocytes in human memory fuctions. Thus, neuro- and immunopeptides serve as a link between the nervous and immune system. Investigation results showed that CSF and blood lymphocytes synth~ize memory immunomodulator Bendorphine and memory mediator vasopressin.

MEASUREMENT OF BODY FLUID-MICROGLOBULIN IN CEREBROVASCULAR DISEASE PATIENTS C h e n Ping, Neurological Department of Hengyang No. 2 Hospital, Hengyang 421001, Hunan, P.R. China Through measuring serum, CSF, urine 1~2-Microglobulin (I~2-MG)in 56 cases of cerebrovascular disease (CVD), we have approached the clinical significance of [~2-MG in CVD. All these cases were in-patients proved by CT. A. CVD group: 56 cases, male 39, female 17. Aged from 40 - 82, of which cerebral embolism 35 cases, cerebral hemorrhage 13, subarachnoid 8. No hepatic, renal disease etc, were involved. B. Normal control ~ronp: 15 case, male 9, female 5. Aged from 30-54. 12~¢ I-[~2-MG RIA kit used, t test was applied. Result: A. CVD group serom, CSF, urine B2-MG were 4254.57.-2021.32, 2889.46.-1104.51,550.18+-315.70 nglml. B. Normal control-group: serum, CSF, urine 2-MG were 2165.10*-712.51,215.72"-112.30, 194.3 l*-90.04ng/ml. Body fluid B2-MG in CVD patients was higher then that of control group. All had significant difference in body fluid B2-MG between bemohagic group and ischemic group. Discussion: Increase of serum B2-MG in CVD patients suggests the possible involvement of immunological factors in CVD pathogenesis. The elevation of ~2-MG in CSF may arise from two routes: on the one hand through the increase of the synthesis of [~2-MGin central nervous system; on the other hand, release of [32-MG into CSF from necrotic necrotic cell membranes. The change of urine [~2-M(3may have positive cerrelation with the total [32MG, because of the saturation of renal tubule reabsorption the excretion of serum [~2-MG through urine increases and the urine 132-MGincreases subsequently.