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Change in sialic acid content of adult mouse submandibular gland mucin following chronic isoproterenol (IPR) treatment
Growth Factors and Hormones (121 -146)
121 TRANSFERRIN SYNTHESIS BY MOUSE EMBRYONAL CELLS. E.D. Adamson and J. Meek.
122 CHANGE IN SIALIC A C I D C O N T E N T OF A D U L T M O U S E S U B M A N D I B U L A R G L A N D M U C I N FOLLOWING C H R O N I C I S O P R O T E R E N O L (IPR) TREATMENT. P.A. Denny, M.S. Y i m and P.C. Denny. School of Dentistry, U n i v e r s i t y of S o u t h e r n California, Los Angeles, CA 90089-0641 C h r o n i c treatment of y o u n g rats or mice with IPR causes p r e c o c i o u s d i f f e r e n t i a t i o n of acinar cells in submandib u l a r glands w h i l e it p r o d u c e s hyperplasia and h y p e r t r o p h y of aeinar cells in adults. Changes in the h i s t o c h e m i c a l s t a i n i n g p r o p e r t i e s of the s e c r e t o r y granules suggest a shift from acidic to neutral carbohydrates. We w o n d e r e d if mucin, a m a j o r s e c r e t o r y p r o t e i n of the s u b m a n d i b u l a r a c i n a r cells, is m o d i f i e d by chronic IPR treatment. Carbohydrates from m u c i n of treated a n i m a l s was analyzed by g.c. The ratio of sialic acid (SA) to g a l a c t o s a m i n e (GALN) in the m u c i n was r e d u c e d 60% in IPR animals, w h i l e the total c o n c e n t r a t i o n of SA per p r o t e i n was more than twice that in controls. These o b s e r v a t i o n s indicate that the p a t t e r n of g l y c g s y l a t i o n is a l t e r e d d u r i n g chronic IPR treatment and that it is not a r e f l e c t i o n of the changes in the total SA c o n t e n t of the gland.
La J o l l a Cancer Research Foundation, 10901 N. Torrey Pines Rd., La J o l l a , C a l i f o r n i a 92037. T r a n s f e r r i n is a necessary component f o r growth of a l l c e l l s in v i t r o and f o r p r o l i f e r a t i n g c e l l s in v i v o , both normal and abnormal. The demands of the embryo f o r i r o n - b e a r i n g t r a n s f e r r i n to synthesize i r o n - c o n t a i n i n g proteins of the c e l l s necessitates the e a r l y synthesis of t r a n s f e r r i n by the embryo. The endoderm l a y e r of the v i s c e r a l yolk sac is the most important source of t r a n s f e r r i n f o r embryogenesis up to the l a s t t h i r d of gestation when the f e t a l l i v e r becomes e q u a l l y important. We have studied the synthesis of t r a n s f e r r i n in mouse teratocarcinoma c e l l s and in f e t a l organs to understand the c e l l types t h a t produce t r a n s f e r r i n and therefore i n f l u e n c e local growth and development. Several t i s s u e s synthesize very small amounts of t r a n s f e r r i n i n c l u d i n g f e t a l lung and spinal cord. Using ELISA, we found t h a t stores of t r a n s f e r r i n are highest in 15th day fetal t i s s u e s ( e s p e c i a l l y the limbbuds) and stores decrease as gestation proceeds. 48S
121 TRANSFERRIN SYNTHESIS BY MOUSE EMBRYONAL CELLS. E.D. Adamson and J. Meek.
122 CHANGE IN SIALIC A C I D C O N T E N T OF A D U L T M O U S E S U B M A N D I B U L A R G L A N D M U C I N FOLLOWING C H R O N I C I S O P R O T E R E N O L (IPR) TREATMENT. P.A. Denny, M.S. Y i m and P.C. Denny. School of Dentistry, U n i v e r s i t y of S o u t h e r n California, Los Angeles, CA 90089-0641 C h r o n i c treatment of y o u n g rats or mice with IPR causes p r e c o c i o u s d i f f e r e n t i a t i o n of acinar cells in submandib u l a r glands w h i l e it p r o d u c e s hyperplasia and h y p e r t r o p h y of aeinar cells in adults. Changes in the h i s t o c h e m i c a l s t a i n i n g p r o p e r t i e s of the s e c r e t o r y granules suggest a shift from acidic to neutral carbohydrates. We w o n d e r e d if mucin, a m a j o r s e c r e t o r y p r o t e i n of the s u b m a n d i b u l a r a c i n a r cells, is m o d i f i e d by chronic IPR treatment. Carbohydrates from m u c i n of treated a n i m a l s was analyzed by g.c. The ratio of sialic acid (SA) to g a l a c t o s a m i n e (GALN) in the m u c i n was r e d u c e d 60% in IPR animals, w h i l e the total c o n c e n t r a t i o n of SA per p r o t e i n was more than twice that in controls. These o b s e r v a t i o n s indicate that the p a t t e r n of g l y c g s y l a t i o n is a l t e r e d d u r i n g chronic IPR treatment and that it is not a r e f l e c t i o n of the changes in the total SA c o n t e n t of the gland.
La J o l l a Cancer Research Foundation, 10901 N. Torrey Pines Rd., La J o l l a , C a l i f o r n i a 92037. T r a n s f e r r i n is a necessary component f o r growth of a l l c e l l s in v i t r o and f o r p r o l i f e r a t i n g c e l l s in v i v o , both normal and abnormal. The demands of the embryo f o r i r o n - b e a r i n g t r a n s f e r r i n to synthesize i r o n - c o n t a i n i n g proteins of the c e l l s necessitates the e a r l y synthesis of t r a n s f e r r i n by the embryo. The endoderm l a y e r of the v i s c e r a l yolk sac is the most important source of t r a n s f e r r i n f o r embryogenesis up to the l a s t t h i r d of gestation when the f e t a l l i v e r becomes e q u a l l y important. We have studied the synthesis of t r a n s f e r r i n in mouse teratocarcinoma c e l l s and in f e t a l organs to understand the c e l l types t h a t produce t r a n s f e r r i n and therefore i n f l u e n c e local growth and development. Several t i s s u e s synthesize very small amounts of t r a n s f e r r i n i n c l u d i n g f e t a l lung and spinal cord. Using ELISA, we found t h a t stores of t r a n s f e r r i n are highest in 15th day fetal t i s s u e s ( e s p e c i a l l y the limbbuds) and stores decrease as gestation proceeds. 48S