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Changes in bone microstructure and serum Tracp-5b level in male rats exposed to cadmium
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Abstracts / Bone 47 (2010) S385–S458
capsules after 4 weeks of treatment. No significant differences were found in any of the outcome measures between the Jingli capsules group and either of the comparison groups. However, during 8 weeks of follow-up after drugs discontinuation, there was a trend of increase in WOMAC scores in each group. Moreover, the effect of the Jingli capsule on OA was not better than Viartril-S, but equal to counter osteophytes capsule after drugs withdrawal. No severe adverse effects were reported. Conclusion: We conclude that Jingli capsule appears to be as effective as commonly prescribed medicines for the relief of pain and dysfunction in knee osteoarthritis patients, but costs a lot less than other Western and herbal drugs in the study. Acknowledgments This work was supported by The National Natural Science Foundation of P.R. China (NO:30300459) and Shanghai Leading Academic Discipline Project (NO:T0303) and the Shanghai Youth Phospherus Project (NO:08QA14063). doi:10.1016/j.bone.2010.09.158
96 Changes in bone microstructure and serum Tracp-5b level in male rats exposed to cadmium Xiao Chen, Guoying Zhu, Chunlin Saho, Taiyi Jin, Mingguang Tan, Shuzhu Gu Institute of Radiation Medicine, Fudan University, Shanghai, China School of Public Health, Fudan University, Shanghai, China Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China Shanghai Public Health Clinical Center, Shanghai, China Objective: Effects of cadmium on bone microstructure and serum Tracp-5b in male rats was investigated in the present study. Material and methods: Twenty-one 8-week-old Sprague-Dawley male rats weighing 180-200 g were randomly divided into 3 groups which were given CdCl2 by subcutaneous injection at the doses of 0 (sodium chloride), 0.1 and 0.5 mg /kg body weight up to 12 weeks, respectively. At the 12th week, before sacrifice, microCT analyses were performed on the proximal tibia for bone microstructure evaluation and urine for 24-h were collected from all of the rats. Then all of the rats were sacrificed by anesthesia and blood was collected from carotid artery for metabolic markers measurement and cadmium assay; lumbar spine (L1-L5) was collected for bone mineral density determination. Right tibia and L3 were dissected for bone histology. Results: The cadmium concentrations in blood, urine and bone of rats treated with cadmium were all significantly higher than those in controls. The bone mineral density and bone mineral ability of rats treated with cadmium at the dose of 0.5 mg/ kg body weight were obviously lower than those treated with sodium chloride. Bone microstructure index (bone fraction volume, trabecular number) and trabecular separation of rats treated with cadmium at 0.5 mg/kg body weight were obviously lower and significantly higher than that in controls, respectively. Histology investigation also revealed the damage of bone microstructure (trabecular conjunction points decreased and dissociated ends increased) caused by cadmium exposure. Tartrate-resistant acid phosphatase 5b (Tracp-5b) levels of rats treated with cadmium were dose-dependent higher than those in controls, but no significant difference was observed in alkaline phosphatase, Ca, P and osteocalcin. Cadmium concentration in blood, urine and bone were significant correlated with Tracp 5b and bone microstructure parameters. Tracp-5b was negatively correlated with number of trabeculae and positively correlated with separation of trabeculae. Conclusion: These observations provide an interpretation for cadmium effect on bone from structural viewpoint. Cadmium could
induce bone microstructure damage, especially to trabecular bone. The increasing of serum Tracp 5b after cadmium exposure prompted that bone reabsorption mediated via osteoclast was an important mechanism of cadmium toxic effects on bone. doi:10.1016/j.bone.2010.09.159
97 Effects of hepcidin on the proliferation and expressions of genes in MC3T3-E1 osteoblasts of mice Hu Liu, Youjia Xu Department of Orthopedics, The Second Hospital Affiliated to Soochow University, Jiangsu Suzhou, China Objective: To investigate the effects of hepcidin on the proliferation and osteoprotegerin (OPG) and osteocalcin (BGP gene expressions in MC3T3-E1 osteoblasts of mice. Methods: After MC3T3-E1 mice cells were cultured with different concentrations of hepcidin (50 nmol/L, 100 nmol/L, 200 nmol/L, 300 nmol/L) for 24, 48 and 72 hours, we detected the proliferation of osteoblasts using MTT. After different concentrations of hepcidin (100 nmol/L, 200 nmol/L, 300 nmol/L) cell for 72 hours, we evaluated the expression of OPG, BGP mRNA using RTPCR. Next, after desferrioxamine (DFO) of different concentrations were added in MC3T3-E1 mice cell cultures in vitro (50 μmol/L, 100 μmol/L, 200 μmol/L, 300 μmol/L) for 24, 48 and 72 hours, we detected the proliferation of osteoblasts using MTT. Then after different concentrations of DFO (50 μmol/L, 100 μmol/L) to the cells for 48 hours, we evaluated the expression of OPG, BGP mRNA using RT-PCR. Results: After the intervention of different concentrations of hepcidin, the result of MTT showed that the growth was upgraded by the adding of hepcidin concertrations, which showed a statistical difference of significance (p < 0.05). Conclusion: Excess iron inhibits cell growth, and Hepcidin, DFO upgraded cell growth, with a dose-dependent effect. Hepcidin upgraded the expression of BGP and OPG mRNA in mice MC3T3-E1 cells. There was a dose-dependent correlation between the concentration of hepcidin and the level of transcription. Acknowledgment Project funded by the Jiangsu Natural Science Foundation (BK2008165).
doi:10.1016/j.bone.2010.09.160
100 The possibility of diagnosing osteoporosis by quantitative ultrasound under loading conditions: Preliminary study Chengrui Liu, Deyu Li, Yubo Fan School of Biological Science and Medical Engineering, Beihang University, Beijing, China Objective: The aim of this study was to explore the possibility of diagnosis osteoporosis under loading conditions by quantitative ultrasound (QUS) for better sensitivity and specificity. Methods: Sixteen, healthy girls under 30 years old (23.4 ± 1.05) were in the control group, 45 postmenopausal women 50-77 years old (64.8 ± 7.07) were divided into 3 groups by the number of years since menopause (YSM) (YSM = 1-10, 11-20, 21-30 for group 1, 2, 3, respectively). Two different loading conditions were implemented: sitting (without loading) and standing (with self-weight loading). Calcaneus QUS parameters under the different loading conditions were measured by Panametrics 5800PR ultrasonic pulser-receiver and a pair of V303-SU
Abstracts / Bone 47 (2010) S385–S458
capsules after 4 weeks of treatment. No significant differences were found in any of the outcome measures between the Jingli capsules group and either of the comparison groups. However, during 8 weeks of follow-up after drugs discontinuation, there was a trend of increase in WOMAC scores in each group. Moreover, the effect of the Jingli capsule on OA was not better than Viartril-S, but equal to counter osteophytes capsule after drugs withdrawal. No severe adverse effects were reported. Conclusion: We conclude that Jingli capsule appears to be as effective as commonly prescribed medicines for the relief of pain and dysfunction in knee osteoarthritis patients, but costs a lot less than other Western and herbal drugs in the study. Acknowledgments This work was supported by The National Natural Science Foundation of P.R. China (NO:30300459) and Shanghai Leading Academic Discipline Project (NO:T0303) and the Shanghai Youth Phospherus Project (NO:08QA14063). doi:10.1016/j.bone.2010.09.158
96 Changes in bone microstructure and serum Tracp-5b level in male rats exposed to cadmium Xiao Chen, Guoying Zhu, Chunlin Saho, Taiyi Jin, Mingguang Tan, Shuzhu Gu Institute of Radiation Medicine, Fudan University, Shanghai, China School of Public Health, Fudan University, Shanghai, China Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China Shanghai Public Health Clinical Center, Shanghai, China Objective: Effects of cadmium on bone microstructure and serum Tracp-5b in male rats was investigated in the present study. Material and methods: Twenty-one 8-week-old Sprague-Dawley male rats weighing 180-200 g were randomly divided into 3 groups which were given CdCl2 by subcutaneous injection at the doses of 0 (sodium chloride), 0.1 and 0.5 mg /kg body weight up to 12 weeks, respectively. At the 12th week, before sacrifice, microCT analyses were performed on the proximal tibia for bone microstructure evaluation and urine for 24-h were collected from all of the rats. Then all of the rats were sacrificed by anesthesia and blood was collected from carotid artery for metabolic markers measurement and cadmium assay; lumbar spine (L1-L5) was collected for bone mineral density determination. Right tibia and L3 were dissected for bone histology. Results: The cadmium concentrations in blood, urine and bone of rats treated with cadmium were all significantly higher than those in controls. The bone mineral density and bone mineral ability of rats treated with cadmium at the dose of 0.5 mg/ kg body weight were obviously lower than those treated with sodium chloride. Bone microstructure index (bone fraction volume, trabecular number) and trabecular separation of rats treated with cadmium at 0.5 mg/kg body weight were obviously lower and significantly higher than that in controls, respectively. Histology investigation also revealed the damage of bone microstructure (trabecular conjunction points decreased and dissociated ends increased) caused by cadmium exposure. Tartrate-resistant acid phosphatase 5b (Tracp-5b) levels of rats treated with cadmium were dose-dependent higher than those in controls, but no significant difference was observed in alkaline phosphatase, Ca, P and osteocalcin. Cadmium concentration in blood, urine and bone were significant correlated with Tracp 5b and bone microstructure parameters. Tracp-5b was negatively correlated with number of trabeculae and positively correlated with separation of trabeculae. Conclusion: These observations provide an interpretation for cadmium effect on bone from structural viewpoint. Cadmium could
induce bone microstructure damage, especially to trabecular bone. The increasing of serum Tracp 5b after cadmium exposure prompted that bone reabsorption mediated via osteoclast was an important mechanism of cadmium toxic effects on bone. doi:10.1016/j.bone.2010.09.159
97 Effects of hepcidin on the proliferation and expressions of genes in MC3T3-E1 osteoblasts of mice Hu Liu, Youjia Xu Department of Orthopedics, The Second Hospital Affiliated to Soochow University, Jiangsu Suzhou, China Objective: To investigate the effects of hepcidin on the proliferation and osteoprotegerin (OPG) and osteocalcin (BGP gene expressions in MC3T3-E1 osteoblasts of mice. Methods: After MC3T3-E1 mice cells were cultured with different concentrations of hepcidin (50 nmol/L, 100 nmol/L, 200 nmol/L, 300 nmol/L) for 24, 48 and 72 hours, we detected the proliferation of osteoblasts using MTT. After different concentrations of hepcidin (100 nmol/L, 200 nmol/L, 300 nmol/L) cell for 72 hours, we evaluated the expression of OPG, BGP mRNA using RTPCR. Next, after desferrioxamine (DFO) of different concentrations were added in MC3T3-E1 mice cell cultures in vitro (50 μmol/L, 100 μmol/L, 200 μmol/L, 300 μmol/L) for 24, 48 and 72 hours, we detected the proliferation of osteoblasts using MTT. Then after different concentrations of DFO (50 μmol/L, 100 μmol/L) to the cells for 48 hours, we evaluated the expression of OPG, BGP mRNA using RT-PCR. Results: After the intervention of different concentrations of hepcidin, the result of MTT showed that the growth was upgraded by the adding of hepcidin concertrations, which showed a statistical difference of significance (p < 0.05). Conclusion: Excess iron inhibits cell growth, and Hepcidin, DFO upgraded cell growth, with a dose-dependent effect. Hepcidin upgraded the expression of BGP and OPG mRNA in mice MC3T3-E1 cells. There was a dose-dependent correlation between the concentration of hepcidin and the level of transcription. Acknowledgment Project funded by the Jiangsu Natural Science Foundation (BK2008165).
doi:10.1016/j.bone.2010.09.160
100 The possibility of diagnosing osteoporosis by quantitative ultrasound under loading conditions: Preliminary study Chengrui Liu, Deyu Li, Yubo Fan School of Biological Science and Medical Engineering, Beihang University, Beijing, China Objective: The aim of this study was to explore the possibility of diagnosis osteoporosis under loading conditions by quantitative ultrasound (QUS) for better sensitivity and specificity. Methods: Sixteen, healthy girls under 30 years old (23.4 ± 1.05) were in the control group, 45 postmenopausal women 50-77 years old (64.8 ± 7.07) were divided into 3 groups by the number of years since menopause (YSM) (YSM = 1-10, 11-20, 21-30 for group 1, 2, 3, respectively). Two different loading conditions were implemented: sitting (without loading) and standing (with self-weight loading). Calcaneus QUS parameters under the different loading conditions were measured by Panametrics 5800PR ultrasonic pulser-receiver and a pair of V303-SU