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Changes in sperm antibody test results when spermatozoa are subjected to capacitating conditions
Citurions from the Lireruruw Evidence that human folliculsr fluid contains a chemoattractant for spermatoz.oa
Villanueva-Diaz C; Vadillo-Ortega F; Kably-Ambe A; D&-Perez MDLA; Krivitzky SK Institute National de Perinatologia, Monres Urales 800, Cot Lomus de Virreyes. CP Iloo Mexico, DF, MEX
FERTIL STERIL 1990, 54/6 (1180-1182) Considering that information from the literature clearly establishes that human follicular fluid enhances both motility and viability of spermatozoa and that this fluid contains substances with chemotactic activity for leukocytes, we looked to see if follicular fluid might exert any chemical attraction to male germinal cells. In an vitro model using 0.8% agarose plates, it was demonstrated that the number of cells migrating to wells containing follicular fluids from patients in a program for GIFT. classified as mature in accordance to oocyte morphology, was significantly higher than those migrating to control wells. Further studies are needed to confirm these results and to study the possible specificity of the signal. Effect of follicular fluid on sperm movement characteristics
Mendoza C; Tesarik J INSERM U 187. Department of Obstetrics and Gynecology, Hopital Antoine-Beclere. 92141 Clamart, FRA
FERTIL STERIL 1990, 5416 (I 135-I 139) Human spermatozoa previously washed from seminal plasma and incubated in capacitating medium were exposed to human follicular fluid (FF) for different time periods and their movement characteristics were examined by computer-assisted digital image analysis. No differences from control samples were observed after short exposures (I 5 mins and 2 h). When the exposure was prolonged to 6 h, spermatozoa incubated in the presence of FF displayed significant differences in some movement characteristics as compared with samples incubated without FF supplement. When only rapidly moving sperm subpopulations were considered, these effects of FF were similar to those produced by a short incubation with the solubilized cumulus oophorus matrix as described previously. After 24 h of incubation, there was a remarkable beneficial effect of FF on motility preservation. Possible physiological and clinical implications are discussed. w in sperm antibody test results when spermatoz.oa are sub jetted to capacitating conditions
Monroe JR; Altenbem DC; Mathur S Department of Obstetrics and Gynecology. Medical University qf South Carolina, I71 Ashley Avenue, Charleston, X29425. DSA
FERTIL STERIL 1990, 54/6 (I 114-l 120) Cytotoxic and immunobead-binding antibody assays were used to document possible changes in antigenicity after the spermatozoa were subjected in vitro to capacitating conditions. Serum and seminal plasma immunobead-binding immunoglobulin (Ig)A. sperm surface IgG, and IgA, and seminal plasma cytotoxic antibody titers in 26 autoimmune infertile men were decreased against autologous spermatozoa under capacitating conditions. Cytotoxic antibodies and immunobead-binding IgG, IgA. and IgM against husbands’ sperm undergoing capacitation, in the
173
cervical mucus of these women, were less than those against uncapacitated sperm. Conversely, sperm antibody levels were increased against the capacitated husbands’ sperm in the husbands’ serum and wives’ cervical mucus, originally testing negative for cytotoxic antibodies against uncapacitated sperm. Antibody levels were unaltered against donors’ sperm undergoing capacitation. except for a reduction in titers in the serum of isoimmune wives. The differences in sperm antibody assay results, depending on whether or not spermatozoa are incubated under conditions favoring capacitation, are specific to the husbands’ and not to the donors’ spermatozoa1 antigens. Fertility prognosis for infertile men: Results of follow-up stmly of semen analysis in infertile men from two different populations evaluated by the Cox regression model
Bostofte E; Bagger P; Michael A; Stakemann G Bredevej 478, 2830 Virum. DNK
FERTIL STERIL 1990, 54/6 (I 100-l 106) Using the Cox proportional hazard regression model on one material (group I = 765, 1950 to 1951) we have identified four variables of semen analysis with significant prognostic information about fertility. The four variables were combined into a model for establishing the probability of the individual male to achieve pregnancy as a function of time. This model is tested first on another material (group II = 321, 1977 to 1985). A Goodness-of-lit test indicates excellent agreement between the expectations from the model and the observed number of pregnancies in group II. Second, the two groups are pooled (= 1,086). Then only three variables give signilicant prognostic information about the time until pregnancy: (I) the man’s age at semen analysis (years); (2) the percentage of morphologically normal spermatozoa (In ‘%I);and (3) the degree of motility (good/poor). These three important variables enter into a new and better prognostic model. A comparison of sperm antibody assays
Daru J; Mathur S Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Medical University qf Sourlt Carolina, I71 Ashley Ave. Charlessron, SC 29425, USA
AM J OBSTET GYNECOL 1990. l63/5 I (1622-1629) Cytotoxicity and Immunobead-binding immunoglobulin G assays agreed in 8 I’%of sera from infertile men (n = 173). 74% of sera from their wives, 75% of seminal plasma. and 82% of cervical mucous samples (p < 0.001; chi’ analysis of positive and negative results) when tested against sperm from the same donors. Cytotoxic and immunofluorescent IgG antibody results agreed in 7% of sera from men and women, 76% of seminal plasma. and 76% of cervical mucus (p < 0.001: chi’ analysis of positive and negative results). Titers or percent binding of sperm by cytotoxicity, hemagglutination. and IgG Immunobead-binding or immunofluorescence assays in serum and secretions of husbands and wives correlated (p < 0.001). A significantly increased number of positive results was observed in cytotoxic and Immunobead-binding IgG assays when the husbands’ and donors’ sperm were used rather than donors’ sperm alone. On the basis of this study we conclude: (I) that cytotoxicity. ImmunobeadInt J Gwecoi
Obstet 36
Villanueva-Diaz C; Vadillo-Ortega F; Kably-Ambe A; D&-Perez MDLA; Krivitzky SK Institute National de Perinatologia, Monres Urales 800, Cot Lomus de Virreyes. CP Iloo Mexico, DF, MEX
FERTIL STERIL 1990, 54/6 (1180-1182) Considering that information from the literature clearly establishes that human follicular fluid enhances both motility and viability of spermatozoa and that this fluid contains substances with chemotactic activity for leukocytes, we looked to see if follicular fluid might exert any chemical attraction to male germinal cells. In an vitro model using 0.8% agarose plates, it was demonstrated that the number of cells migrating to wells containing follicular fluids from patients in a program for GIFT. classified as mature in accordance to oocyte morphology, was significantly higher than those migrating to control wells. Further studies are needed to confirm these results and to study the possible specificity of the signal. Effect of follicular fluid on sperm movement characteristics
Mendoza C; Tesarik J INSERM U 187. Department of Obstetrics and Gynecology, Hopital Antoine-Beclere. 92141 Clamart, FRA
FERTIL STERIL 1990, 5416 (I 135-I 139) Human spermatozoa previously washed from seminal plasma and incubated in capacitating medium were exposed to human follicular fluid (FF) for different time periods and their movement characteristics were examined by computer-assisted digital image analysis. No differences from control samples were observed after short exposures (I 5 mins and 2 h). When the exposure was prolonged to 6 h, spermatozoa incubated in the presence of FF displayed significant differences in some movement characteristics as compared with samples incubated without FF supplement. When only rapidly moving sperm subpopulations were considered, these effects of FF were similar to those produced by a short incubation with the solubilized cumulus oophorus matrix as described previously. After 24 h of incubation, there was a remarkable beneficial effect of FF on motility preservation. Possible physiological and clinical implications are discussed. w in sperm antibody test results when spermatoz.oa are sub jetted to capacitating conditions
Monroe JR; Altenbem DC; Mathur S Department of Obstetrics and Gynecology. Medical University qf South Carolina, I71 Ashley Avenue, Charleston, X29425. DSA
FERTIL STERIL 1990, 54/6 (I 114-l 120) Cytotoxic and immunobead-binding antibody assays were used to document possible changes in antigenicity after the spermatozoa were subjected in vitro to capacitating conditions. Serum and seminal plasma immunobead-binding immunoglobulin (Ig)A. sperm surface IgG, and IgA, and seminal plasma cytotoxic antibody titers in 26 autoimmune infertile men were decreased against autologous spermatozoa under capacitating conditions. Cytotoxic antibodies and immunobead-binding IgG, IgA. and IgM against husbands’ sperm undergoing capacitation, in the
173
cervical mucus of these women, were less than those against uncapacitated sperm. Conversely, sperm antibody levels were increased against the capacitated husbands’ sperm in the husbands’ serum and wives’ cervical mucus, originally testing negative for cytotoxic antibodies against uncapacitated sperm. Antibody levels were unaltered against donors’ sperm undergoing capacitation. except for a reduction in titers in the serum of isoimmune wives. The differences in sperm antibody assay results, depending on whether or not spermatozoa are incubated under conditions favoring capacitation, are specific to the husbands’ and not to the donors’ spermatozoa1 antigens. Fertility prognosis for infertile men: Results of follow-up stmly of semen analysis in infertile men from two different populations evaluated by the Cox regression model
Bostofte E; Bagger P; Michael A; Stakemann G Bredevej 478, 2830 Virum. DNK
FERTIL STERIL 1990, 54/6 (I 100-l 106) Using the Cox proportional hazard regression model on one material (group I = 765, 1950 to 1951) we have identified four variables of semen analysis with significant prognostic information about fertility. The four variables were combined into a model for establishing the probability of the individual male to achieve pregnancy as a function of time. This model is tested first on another material (group II = 321, 1977 to 1985). A Goodness-of-lit test indicates excellent agreement between the expectations from the model and the observed number of pregnancies in group II. Second, the two groups are pooled (= 1,086). Then only three variables give signilicant prognostic information about the time until pregnancy: (I) the man’s age at semen analysis (years); (2) the percentage of morphologically normal spermatozoa (In ‘%I);and (3) the degree of motility (good/poor). These three important variables enter into a new and better prognostic model. A comparison of sperm antibody assays
Daru J; Mathur S Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Medical University qf Sourlt Carolina, I71 Ashley Ave. Charlessron, SC 29425, USA
AM J OBSTET GYNECOL 1990. l63/5 I (1622-1629) Cytotoxicity and Immunobead-binding immunoglobulin G assays agreed in 8 I’%of sera from infertile men (n = 173). 74% of sera from their wives, 75% of seminal plasma. and 82% of cervical mucous samples (p < 0.001; chi’ analysis of positive and negative results) when tested against sperm from the same donors. Cytotoxic and immunofluorescent IgG antibody results agreed in 7% of sera from men and women, 76% of seminal plasma. and 76% of cervical mucus (p < 0.001: chi’ analysis of positive and negative results). Titers or percent binding of sperm by cytotoxicity, hemagglutination. and IgG Immunobead-binding or immunofluorescence assays in serum and secretions of husbands and wives correlated (p < 0.001). A significantly increased number of positive results was observed in cytotoxic and Immunobead-binding IgG assays when the husbands’ and donors’ sperm were used rather than donors’ sperm alone. On the basis of this study we conclude: (I) that cytotoxicity. ImmunobeadInt J Gwecoi
Obstet 36