Changes in the EEG activity of the anterior hypothalamus and sensorimotor cortex during the immune response in the rat

!72 CHANGES IN THE EEG ACTIVITY OF THE ANTERIOR HYPOTHALAMUS ANz"DSENSORIMOTCJR ZORTEX DURING THE IMMUNE RESPONSE IN THE RAT Katica Jovanova-Nesic*, B. Jankovi~: °. Milka Culi6 °, Vera Nikoli¢*, V. Savi6*, and B.D. Jankovi~:*, Immunology Research Center, Bdgrade, Yugoslavia* Center for Multidisciplinary Study, University of Belgrade ° To evaluate EEG signals from anterior hypothalamus and se.~sorimotor cortex in the rats, before and 30 min, 1, 3, 7, and 14 days after the immunhation with bovine serum albumin (BSA) in complete Freund's adju;'.~m (CFA), several procedures for the preparation and further processing acquired signals and date analysing have been developed. Stationary bipolar EEG electrodes (stainless steel wires) were placed stereotaxically in the above mentioned brain structures, ander Nembutal anaesthesia (40 mg/kg, i.p.), using coordinates described in Paxinos and Watson atlas of the rat brain. All procedures regarding with the spectral analysis of EEG signals are performed on the computer DECLAB 11/40 and laboratory peripheral unit of DECLAB 11/40, as separate statements of special problemoriented language, for application in neurophysiology. The procedures provide a standard frequency analysis based on the Fast Fourier Transform for previously accepted, calibrated and converted continous EEG signals and the power spectrum of these signals. The differences between the date are statistically estimated using two-way and one-way ANOVA. Immunization with BSA in CFA decreased the power contribution in the EEG of the anterior hypothalamus and sensotimotor cortex 30 min and 1st clay after the immunization. However, on day 3, the power contribution was moderately increased, while 7th and 14th day was significantly increased, compared to those before the immunization. The frequency of these signals were moderately increased after the immunization, and these changes persisted during the whole experiment, and in the both anterior hypothalamus and sensorimotor cortex. (Supported by the Republic of Serbia Fund, Belgrade) DEEP STRUCTURES OF THE BRAIN ARE INVOLVED IN THE MODULATION OF THE IMMUNE RESPONSE TO THE TRANSPLANTATION OF TUMOR CELLS V.Vinnitsky, S.Prokopovich. Institute for Oncology Problems of the Academy of Science of the Ukranian SSR, Kiev, USSR Rabbits with transplanted cells of Brown-Pierce carcinoma under/want the implantation of golden electrodes into 8 brain structures: area hypothalamica posterior, area preoptica medialis, nucleus ventromedialis of the hypothalamus, hippocampus (area CA-2), amygdala basalis, amygdala lateralis, nucleus raphus cent~alis, locus coeruleus with simultaneous registration of the DC-potentials of these structures. Immunodependent pattern of tumor growth (progressive or regressive) correlated with the spatial-tempo~ai organization of the activity of subcortical structures. Under spontaneous tumor regression (subcdtaneous transplantation) two waves of synchronous changes in the DC-potential were registered. Rabbits with rapi0, and s~,owtumor regression revealed the maximal activity in d'fferent subeortical structures. Under secondary immune response to repeated t.rausplantation o~'*,~'~,)rcells the alterations in DC-potential were registered only in the hippocampus (~rea CA-2) and in the area preoptica medialis of the hypothalamus. Under progressive tumor growth (i~tratesticular transplantation) brain structures revealed chaotic alterations in the'.DC-Fetential in the absence of synchronous, organized :reactions. Immunization of rabl:!,'.swith test antigens of nontumoral nature was accompanied by a principally diff~.r~.ntspaual-temporal pattern of the DC-potential in the studied brain structures.