- Email: [email protected]
Changes in types of collagen in the process of burn wound healing in guinea pigs
134
Effect of dermatitis
topical application in the guinea pig.
of
cyclosporin
A
CHANGES IN TYPES OF COLLAGEN IN THE PROCESS OF BURN WOUND HEALING IN GUINEA PIGS M. Baba, K. Yamanishi, S. Kishimoto and H. Yasuno. Department Dermatology, Kyoto Prefectural University of Medicine.Kyoto
on contact
Daisuke OKA, Masako YOKOO, Shojiro NAKAGAWA and Hiroaki UEKI, Department of Dermatology, Kawasaki Medical School, 577 Matsushima Kurashiki, 701-01 Japan
Type I and type III collagen are major structural molecules of the dermis, generated by fibroblasts or mesenchymal cells. To understand the role of these two types of collagen in the burn-wound healing, we examined alteration in the ratio of type III to type I collagen and morphological changes of collagen after experimental burn in guinea pigs.
Cyclasporin A (CYA) seems attractive for the treatment of patients with various cutaneous immune-mediated disorders. We have previously reported the effect of the guinea pigs allergic contact dermatitis to DNCB. In the present study, the effect of topically administered CYA was evaluated using an animal model of toxic contact dermatitis to croton oil. Topical CYA applied to the test site substantially inhibited the toxic reaction to croton ail. The suppressive effect was short lived and reversible. CYA has not only an effect on allergic but also an effect on toxic contact dermatitis. These results suggest that topical CYA may become useful in the therapy of human contact dermatitis.
IMPROVEMENT Z. Ikezawa, Dermatology,
EFFECT
ON PSORIASIS
BY CYCLOSPORIN
T. Sasaki, S. Saito and 8. Nakajima. Yokohama City Univ. School of Med.
I
.
of
.
1.
K. Kosaka, T. of Dermatology,
OF THE SKIN
DERMAL
The relative proportion of type III to type I collagen was 9.0% before burn. It increased after burn and the peak (14.8%) was found on 21 days after burn. Thereafter it decreased gradually up In comparison with histological findings, the to 60 days (10.6%). increase in the ratio may be characteristic of both granulation and early stage of scar,
I DISTRIBUTION PATTERNS OF TYPE I III,IV,V COLLAGENS LAMININ IN MELANOCYTIC TUMOR AND N~UROFIBROMA --IlQ6UNOHISTOCHEMICAL STUDY USING POLYCLONAL MONOCLONAL ANTIBQDIES--
AND AND
-
Thi/Ts and Thi/Tc was decreased. Th CD4' , HLA-DR+ activated Th-CD8+, HLA-DR+ Tslc cell ratio was also decreased. These results were consistent with -in vitro studies of CsA reported before and might well explain improvement effect by CsA treatment observed in this study.
IMMUNOHISTOCHEMISTRY IN THE NORMAL HUMAN
The wounds of deep burn were made on the bilateral thighs of The animals were sacrificed on 4, 7, 14, 21, 28 and quinea pigs. The excised skin samples were solubilized by 60 days after burn. pepsin digestion and, after addition of 2 M N&l, the precipitated proteins were analysed by interrupted gel electrophoresis.
A
Department
I1 patients with psoriasis were treated only with oral administration of Smglkglday of cyclosporin A(CsA) twice a day for 3 months, durine which thev were examined includine sinele- and twocolor analysis of lymphocyte surface markers by flow cytometry using monaclonal antibodies. 5 patients showed improvement within a week and the others within a month. Four patients had total remission, 3 showed marked improvement and 4 showed moderate improvement, while no significant side effects were seen. In peripheral blood of patients e was a decrease in both-CD4t,4B4f helpe; inducer suppressor inducer T(Tsi) cells, the former However, chere+was n_o signifibeing more remarkable+than ghe latter. cant decrease in CD11 CD8 suppressor TITS) and CD11 CD8 cytotoxic T(Tc) cells. As these results each ratio of Thi/Tsi, ”
of
ELASTIC
SYSTEM
OM.Chanoki, M.Ishii, H.Kobayashi, K.Fukai and T.Hamada. Dept. of Dermatology, Osaka City Univ. Med. School. Y.Muragaki and A.Ooshima. First Dept. of Pathology, Wakayama Med. School. The distrubution of type I,III,IV,V collagens and laminin in melanocytic nevi(MN), malignant melanoma(MM), blue nevi(BN), nevus of Ota(NO), neurofibromas(NF) and neurofibrosarcoma(NFS) has been examined using specific antibodies. Type IV collagen and laminin were not detected in epidermal news cells of MN and dermal melanocvtes of BN and NO. Most of intradermal nevus cells or cell nests ;ere surrounded by type IV collagen and laminin but some did not have basement membrane(bm) components. Tumor cells of MM had various patterns of bm components. Type I,III,V collagen6 were irregularly embedded around melanoma cells. In NF, I,III,V collagens were distributed among the collagen bundles and bm components were seen in a linear or spindle pattern. In NFS,tumor cells contained less bm components than NF.
FIBERS
Tsuji, K. Shindo and T. Iiamada. Department Osaka City University Medical School., Osaka.
Human dermal elastic system is consist of three types of fibers: oxytalan, elaunin and elastic. Ultrastructurally, the oxytalan fibers appear 8.9 a bundle of microfibrils, but without amorphous material. The elaunin fibers present as a dispersed amorphous material intermingled with the microfibrils. The elastic fibers have amorphous material in the center and microfibrils at the periphery. Ismunohistochemical studies by light and electron microscopy revealed that anti-elastin, antiSAP and HE8 antibodies reacted with each of the elastic system fibers. After elastase-digestion, the anti-elastin antibody did not reacted with any of the elastic system fibers, while the anti-SAP and HB8 antibodies reacted with them. It is suggested that the oxytalan fibers contain amorphous materials ( elastin ) as well as microfibrils as the elaunin and elastic fibers do.
ENZYMATIC CHARACTERIZATION -PRESENCE OF ITS ISOZYMES-
OF FIBROBLASTS-DERIVED
T. Oono, J. Arata, T. Ohhashi* and H. Kodama* Department of Dermatology and Chemistry*, Kochi Nankoku-shi, Kochi.
Medical
PROLIDASE
School,
Isozyres of Prolidase(Pd-1,II) from cultured human skin fibroblasts were separated by DEAE-5PW column "PLC. In control, activities of Pd -I were decreased in the descending order of activities against Gly -Pro, Ala-Pro, Met-Pro. However, activities of Pd-II were decreased in the descending order of activities against Met-Pro, Gly-Pro, AlaPro. In prolidase deficient sisters(syqtom(+), symptom(-)), the activities of Pd-I could not be detected, while the activities of Pd -II were present. The activities of Pd-II of prolidase-deficient sisters markedlv increased aeainst Met-Pro. No remarkable differences were seen between the substrate specificities of the Pd-II from symptom(+)pati and symptom(-)sist
Effect of dermatitis
topical application in the guinea pig.
of
cyclosporin
A
CHANGES IN TYPES OF COLLAGEN IN THE PROCESS OF BURN WOUND HEALING IN GUINEA PIGS M. Baba, K. Yamanishi, S. Kishimoto and H. Yasuno. Department Dermatology, Kyoto Prefectural University of Medicine.Kyoto
on contact
Daisuke OKA, Masako YOKOO, Shojiro NAKAGAWA and Hiroaki UEKI, Department of Dermatology, Kawasaki Medical School, 577 Matsushima Kurashiki, 701-01 Japan
Type I and type III collagen are major structural molecules of the dermis, generated by fibroblasts or mesenchymal cells. To understand the role of these two types of collagen in the burn-wound healing, we examined alteration in the ratio of type III to type I collagen and morphological changes of collagen after experimental burn in guinea pigs.
Cyclasporin A (CYA) seems attractive for the treatment of patients with various cutaneous immune-mediated disorders. We have previously reported the effect of the guinea pigs allergic contact dermatitis to DNCB. In the present study, the effect of topically administered CYA was evaluated using an animal model of toxic contact dermatitis to croton oil. Topical CYA applied to the test site substantially inhibited the toxic reaction to croton ail. The suppressive effect was short lived and reversible. CYA has not only an effect on allergic but also an effect on toxic contact dermatitis. These results suggest that topical CYA may become useful in the therapy of human contact dermatitis.
IMPROVEMENT Z. Ikezawa, Dermatology,
EFFECT
ON PSORIASIS
BY CYCLOSPORIN
T. Sasaki, S. Saito and 8. Nakajima. Yokohama City Univ. School of Med.
I
.
of
.
1.
K. Kosaka, T. of Dermatology,
OF THE SKIN
DERMAL
The relative proportion of type III to type I collagen was 9.0% before burn. It increased after burn and the peak (14.8%) was found on 21 days after burn. Thereafter it decreased gradually up In comparison with histological findings, the to 60 days (10.6%). increase in the ratio may be characteristic of both granulation and early stage of scar,
I DISTRIBUTION PATTERNS OF TYPE I III,IV,V COLLAGENS LAMININ IN MELANOCYTIC TUMOR AND N~UROFIBROMA --IlQ6UNOHISTOCHEMICAL STUDY USING POLYCLONAL MONOCLONAL ANTIBQDIES--
AND AND
-
Thi/Ts and Thi/Tc was decreased. Th CD4' , HLA-DR+ activated Th-CD8+, HLA-DR+ Tslc cell ratio was also decreased. These results were consistent with -in vitro studies of CsA reported before and might well explain improvement effect by CsA treatment observed in this study.
IMMUNOHISTOCHEMISTRY IN THE NORMAL HUMAN
The wounds of deep burn were made on the bilateral thighs of The animals were sacrificed on 4, 7, 14, 21, 28 and quinea pigs. The excised skin samples were solubilized by 60 days after burn. pepsin digestion and, after addition of 2 M N&l, the precipitated proteins were analysed by interrupted gel electrophoresis.
A
Department
I1 patients with psoriasis were treated only with oral administration of Smglkglday of cyclosporin A(CsA) twice a day for 3 months, durine which thev were examined includine sinele- and twocolor analysis of lymphocyte surface markers by flow cytometry using monaclonal antibodies. 5 patients showed improvement within a week and the others within a month. Four patients had total remission, 3 showed marked improvement and 4 showed moderate improvement, while no significant side effects were seen. In peripheral blood of patients e was a decrease in both-CD4t,4B4f helpe; inducer suppressor inducer T(Tsi) cells, the former However, chere+was n_o signifibeing more remarkable+than ghe latter. cant decrease in CD11 CD8 suppressor TITS) and CD11 CD8 cytotoxic T(Tc) cells. As these results each ratio of Thi/Tsi, ”
of
ELASTIC
SYSTEM
OM.Chanoki, M.Ishii, H.Kobayashi, K.Fukai and T.Hamada. Dept. of Dermatology, Osaka City Univ. Med. School. Y.Muragaki and A.Ooshima. First Dept. of Pathology, Wakayama Med. School. The distrubution of type I,III,IV,V collagens and laminin in melanocytic nevi(MN), malignant melanoma(MM), blue nevi(BN), nevus of Ota(NO), neurofibromas(NF) and neurofibrosarcoma(NFS) has been examined using specific antibodies. Type IV collagen and laminin were not detected in epidermal news cells of MN and dermal melanocvtes of BN and NO. Most of intradermal nevus cells or cell nests ;ere surrounded by type IV collagen and laminin but some did not have basement membrane(bm) components. Tumor cells of MM had various patterns of bm components. Type I,III,V collagen6 were irregularly embedded around melanoma cells. In NF, I,III,V collagens were distributed among the collagen bundles and bm components were seen in a linear or spindle pattern. In NFS,tumor cells contained less bm components than NF.
FIBERS
Tsuji, K. Shindo and T. Iiamada. Department Osaka City University Medical School., Osaka.
Human dermal elastic system is consist of three types of fibers: oxytalan, elaunin and elastic. Ultrastructurally, the oxytalan fibers appear 8.9 a bundle of microfibrils, but without amorphous material. The elaunin fibers present as a dispersed amorphous material intermingled with the microfibrils. The elastic fibers have amorphous material in the center and microfibrils at the periphery. Ismunohistochemical studies by light and electron microscopy revealed that anti-elastin, antiSAP and HE8 antibodies reacted with each of the elastic system fibers. After elastase-digestion, the anti-elastin antibody did not reacted with any of the elastic system fibers, while the anti-SAP and HB8 antibodies reacted with them. It is suggested that the oxytalan fibers contain amorphous materials ( elastin ) as well as microfibrils as the elaunin and elastic fibers do.
ENZYMATIC CHARACTERIZATION -PRESENCE OF ITS ISOZYMES-
OF FIBROBLASTS-DERIVED
T. Oono, J. Arata, T. Ohhashi* and H. Kodama* Department of Dermatology and Chemistry*, Kochi Nankoku-shi, Kochi.
Medical
PROLIDASE
School,
Isozyres of Prolidase(Pd-1,II) from cultured human skin fibroblasts were separated by DEAE-5PW column "PLC. In control, activities of Pd -I were decreased in the descending order of activities against Gly -Pro, Ala-Pro, Met-Pro. However, activities of Pd-II were decreased in the descending order of activities against Met-Pro, Gly-Pro, AlaPro. In prolidase deficient sisters(syqtom(+), symptom(-)), the activities of Pd-I could not be detected, while the activities of Pd -II were present. The activities of Pd-II of prolidase-deficient sisters markedlv increased aeainst Met-Pro. No remarkable differences were seen between the substrate specificities of the Pd-II from symptom(+)pati and symptom(-)sist